Sanro Tachibana
Department of Applied Biosciences, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi Matsuyama, Ehime 790-8566

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DECOLORIZATION OF AZO DYES AND MINERALIZATION OF PHENANTHRENE BY TRAMETES SP. AS03 ISOLATED FROM INDONESIAN MANGROVE FOREST Hidayat, Asep; Tachibana, Sanro
Indonesian Journal of Forestry Research Vol 1, No 1 (2014): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

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Abstract

Textile industry contributes the most disposals of synthetic dyes, and about 40% of textile dyes has been generating high amount of colored wastewater. Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene, is a group of organic compounds, that structurally comprised of two or more benzene rings, which persist in air, water, and soil. The organic pollutants of dyes and PAHs have adversely effects the food chain and are potentially toxic, mutagenic, and carcinogenic to the environment. The objective of this research is to screen and investigate the potential fungus from mangrove forest to degrade azo dyes and phenanthrene.  In this study, fungi were collected from mangrove forest in Riau Province – Sumatra – Indonesia. Previously, Trametes sp. AS03 is one of the fungi isolated from mangrove forest in Riau Province, that was able to decolorize Remazol Brilliant Blue R (RBBR). The capability of Trametes sp. AS03 to decolorize four azo dyes, Remazol B. Violet (V5), Levafix Orange E3GA (Or64), Levafix B. Red E-6BA (R159), and Sumifix S. Scarlet 2GF (R222), were further evaluated. The result shows that Trametes sp. AS03 decolorized 91, 60, 48, and 31 of V5, R222, R159, and Or64, respectively. By showing its capability to decolorize some of the dyes, Trametes sp. AS03 was used to break down phenanthrene. AS03 degraded more than 70% of phenanthrene in 15 days.
Decolorization of Remazol Briliant Blue R by Laccase from White Rot Fungus Polyporus sp. S133 Hadibarata, Tony; Tachibana, Sanro
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

The decolourization of the recalcitrant dye RBBR by the culture filtrate of Polyporus sp. S133 and its isolatedlaccase was investigated. The laccase alone decolorized RBBR. A small molecular weight redox mediator (HBT) wasnecessary to increase the decolorization. The purified laccase totally decolorized the dye of 200 mg l-1 initialconcentration of RBBR when only 1.5 U ml-1 of laccase was used in the reaction mixture. The effects of differentphysicochemical parameters were tested and optimal decolorization rates occurred at pH 5 and at a temperature of 50°C. The effect of surfactants on the decolourization of RBBR was tested with Tween 80, Tween 20, and Brij 35. It wasdemonstrated that Tween 80 was inhibiting substrate for the decolorization while Tween 80 and Brij 35 was noinhibiting effect for the decolorization. Provided that all of the condition is included, it is suggested that laccase maybe suitable for the wastewater treatment of similar anthraquinone dyes.Keywords: Decolorization; Laccase; Remazol Brilliant Blue R (RBBR); Polyporus sp. S133
CHEMICAL COMPOSITIONS OF PINE RESIN, ROSIN AND TURPENTINE OIL FROM WEST JAVA Wiyono, Bambang; Tachibana, Sanro; Tinambunan, Djaban
Indonesian Journal of Forestry Research Vol 3, No 1 (2006): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2006.3.1.7-17

Abstract

This study was conducted to identify chemical composition of merkus pine resin, rosin and turpentine oil. Initially, pine resin was separated into neutral and acidic fractions with an aqueous 4% sodium hydroxide solution. After methylation, the fraction containing turpentine oil and rosin were analyzed by gas chromatography (GC), and gas chromatograph mass spectrometry (GC-MS), respectively. The neutral fraction of pine resin and turpentine oil mainly consisted of a-pinene, D-3-carene and b-pinene. Based on mass spectral comparison, the major constituents of the acidic fraction and rosin were identified as sandaracopimaric acid, isopimaric acid, palustric acid, dehydroabietic acid, abietic acid, neoabietic acid, and merkusic acid. The major component of the acidic fractions was palustric acid, while that of rosin was abietic acid. Using TC (tough column) 1 and TC 5 columns, levopimaric acid could not be separated from rosin or acidic fraction of pine resin of Indonesian Pinus merkusii.
Effect of Piperonyl Butoxide and Silver Nitrate on Degradation Pathways of n-octadecane by Fusarium sp. F092 Hidayat, Asep; Tachibana, Sanro
Indonesian Journal of Forestry Research Vol 3, No 1 (2016): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2016.3.1.%p

Abstract

The aliphatic fraction is usually the largest component in crude oil. Its removal from oil contaminated fields has become an environmental priority and been considered useful for enhancing recovery. Our previous studies reported the isolation of Fusarium sp. F092 based on the ability to degrade chrysene. It also could degrade crude oils and their aliphatics fractions. However, aliphatic degradative pathways in crude oil have not been clearly understood. The identification of aliphatic metabolite pathways using a representative compound n-octadecane was carried out in this study, as well as the effect of Piperonyl Butoxide (PB) and Silver Nitrate (AgNO3) on the degradation of n-octadecane and its metabolite. We determined that Fusarium sp. F092 had ability to break down n-Octadecane from about 125 to 13 mg L-1 after 60 days incubation. During degradation, several metabolite products could be detected and identified to form carboxylic acid groups. By the addition of PB and AgNO3, inhibitor of monooxgenase and dioxygenase enzymes, Fusarium sp. F092 had ability to convert n-octadecane to form alkyl hydroperoxides via terminal oxidation pathway with involving a dioxygenase
DECOLORIZATION OF AZO DYES AND MINERALIZATION OF PHENANTHRENE BY TRAMETES SP. AS03 ISOLATED FROM INDONESIAN MANGROVE FOREST Hidayat, Asep; Tachibana, Sanro
Indonesian Journal of Forestry Research Vol 1, No 1 (2014): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2014.1.1.67-75

Abstract

Textile industry contributes the most disposals of synthetic dyes, and about 40% of textile dyes has been generating high amount of colored wastewater. Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene, is a group of organic compounds, that structurally comprised of two or more benzene rings, which persist in air, water, and soil. The organic pollutants of dyes and PAHs have adversely effects the food chain and are potentially toxic, mutagenic, and carcinogenic to the environment. The objective of this research is to screen and investigate the potential fungus from mangrove forest to degrade azo dyes and phenanthrene.  In this study, fungi were collected from mangrove forest in Riau Province – Sumatra – Indonesia. Previously, Trametes sp. AS03 is one of the fungi isolated from mangrove forest in Riau Province, that was able to decolorize Remazol Brilliant Blue R (RBBR). The capability of Trametes sp. AS03 to decolorize four azo dyes, Remazol B. Violet (V5), Levafix Orange E3GA (Or64), Levafix B. Red E-6BA (R159), and Sumifix S. Scarlet 2GF (R222), were further evaluated. The result shows that Trametes sp. AS03 decolorized 91, 60, 48, and 31 of V5, R222, R159, and Or64, respectively. By showing its capability to decolorize some of the dyes, Trametes sp. AS03 was used to break down phenanthrene. AS03 degraded more than 70% of phenanthrene in 15 days.
DECOLORIZATION OF AZO DYES AND MINERALIZATION OF PHENANTHRENE BY TRAMETES SP. AS03 ISOLATED FROM INDONESIAN MANGROVE FOREST Hidayat, Asep; Tachibana, Sanro
Indonesian Journal of Forestry Research Vol. 1 No. 1 (2014): Indonesian Journal of Forestry Research
Publisher : Association of Indonesian Forestry and Environment Researchers and Technicians

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59465/ijfr.2014.1.1.67-75

Abstract

Textile industry contributes the most disposals of synthetic dyes, and about 40% of textile dyes has been generating high amount of colored wastewater. Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene, is a group of organic compounds, that structurally comprised of two or more benzene rings, which persist in air, water, and soil. The organic pollutants of dyes and PAHs have adversely effects the food chain and are potentially toxic, mutagenic, and carcinogenic to the environment. The objective of this research is to screen and investigate the potential fungus from mangrove forest to degrade azo dyes and phenanthrene. In this study, fungi were collected from mangrove forest in Riau Province – Sumatra – Indonesia. Previously, Trametes sp. AS03 is one of the fungi isolated from mangrove forest in Riau Province, that was able to decolorize Remazol Brilliant Blue R (RBBR). The capability of Trametes sp. AS03 to decolorize four azo dyes, Remazol B. Violet (V5), Levafix Orange E3GA (Or64), Levafix B. Red E-6BA (R159), and Sumifix S. Scarlet 2GF (R222), were further evaluated. The result shows that Trametes sp. AS03 decolorized 91, 60, 48, and 31 of V5, R222, R159, and Or64, respectively. By showing its capability to decolorize some of the dyes, Trametes sp. AS03 was used to break down phenanthrene. AS03 degraded more than 70% of phenanthrene in 15 days.