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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Pengajaran MIPA Indonesian Journal of Biotechnology Jurnal Inovasi Pendidikan IPA Jurnal Kelautan Tropis IJIET (International Journal of Indonesian Education and Teaching) Environmental and Toxicology Management (ETM) Indonesian Journal of Oceanography Industrial and Domestic Waste Management Acta Pedagogia Asiana
Tony Hadibarata, Tony
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Public Health Other

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Decolorization of Remazol Briliant Blue R by Laccase from White Rot Fungus Polyporus sp. S133 Hadibarata, Tony; Tachibana, Sanro
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.014 KB)

Abstract

The decolourization of the recalcitrant dye RBBR by the culture filtrate of Polyporus sp. S133 and its isolatedlaccase was investigated. The laccase alone decolorized RBBR. A small molecular weight redox mediator (HBT) wasnecessary to increase the decolorization. The purified laccase totally decolorized the dye of 200 mg l-1 initialconcentration of RBBR when only 1.5 U ml-1 of laccase was used in the reaction mixture. The effects of differentphysicochemical parameters were tested and optimal decolorization rates occurred at pH 5 and at a temperature of 50°C. The effect of surfactants on the decolourization of RBBR was tested with Tween 80, Tween 20, and Brij 35. It wasdemonstrated that Tween 80 was inhibiting substrate for the decolorization while Tween 80 and Brij 35 was noinhibiting effect for the decolorization. Provided that all of the condition is included, it is suggested that laccase maybe suitable for the wastewater treatment of similar anthraquinone dyes.Keywords: Decolorization; Laccase; Remazol Brilliant Blue R (RBBR); Polyporus sp. S133
Identification of Metabolic Intermediates in Microbial Degradation of Chrysene by Armillaria sp. F022 Hadibarata, Tony; Kristanti, Risky Ayu
Indonesian Journal of Biotechnology Vol 15, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.989 KB)

Abstract

To degrade chrysene, a polycyclic aromatic hydrocarbon (PAH), Armillaria sp. F022, a fungus collected from a soil, was used. Maximal degradation (77%) was obtained when Armillaria sp. F022 was incubated in cultures agitated at 120 rpm for 30 days, as compared to just 41% degradation in stationary culture. Furthermore, the degradation of chrysene was affected by the addition of surfactants. The mechanism of degradation was determined through identification of the intermediates. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase) produced by Armillaria sp. F022 were detected in the culture. The highest level of activity was shown by 1,2-dioxygenase after 20 days (143.6 U l-1). Theseligninolytic and dioxygenase enzymes played an important role in the oxidation of chrysene. Chrysene was indeed degraded by Armillaria sp. F022 through several intermediates, chrysenequinone, 2-((1E,3E)-4-carboxy-3-hydroxybuta-1,3-dien-1-yl)-1-naphthoic acid , 1-hydroxy-2-naphthoic acid, and gentisic acid.Keywords : Biodegradation, Chrysene, Metabolites, Armillaria sp. F022
ENGINEERING STUDENTS’ PERCEPTION ABOUT LEARNING AND ITS VARIOUS TYPES OF ASSESSMENT Hadibarata, Tony; Syafiuddin, Achmad
Jurnal Pengajaran MIPA Vol 23, No 1 (2018): JPMIPA: Volume 23, Issue 1, 2018
Publisher : Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18269/jpmipa.v23i1.8930

Abstract

Students’ perception of assessments can serve as a guiding light for improving overall educational practices. A questionnaire was collected from 80 engineering student to discover students' perceptions of different types of assessment and their overall perceptions of various type of assessments advantages in enhancing learning experiences. The majority of students strongly agreed that quiz and case study was beneficial and helpful for enhancing learning experience in engineering courses. On the contrary, project presentation, journal review, essay, interview, and demonstration was seemed unhelpful for students to enhance their learning experience. In terms of enjoyment, a discrepancy was found for what engineering students’ perceived as important assessment tools for learning and what they actually enjoy doing.
TEACHING GREEN ENGINEERING PRINCIPLES AND APPLICATION THROUGH ACTIVE LEARNING Hadibarata, Tony
International Journal of Indonesian Education and Teaching (IJIET) Vol 3, No 2 (2019): July 2019
Publisher : Sanata Dharma University Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477.639 KB) | DOI: 10.24071/ijiet.v3i2.1746

Abstract

Active learning refers any approach to instruction in which all students are required to involve in the learning process. The purpose of the manuscript is to evaluate the application of active learning in teaching Green Engineering Principles and Applications as a compulsory course in environmental engineering department curriculum, Curtin University Malaysia. Green engineering can be defined as an approach of the design, process, product and commercialization that following environmentally conscious attitude, principles and values combined with multi-disciplinary engineering science that to minimize pollutant and promote local and global sustainability. Green engineering encompasses the conceptualization and implementation of reducing environmental impacts, maximize energy efficiency and develop the greener processes and product that bring environmental and economic benefit. A simple approach that combining the classical lecture-presentation and active engagement of the students with the course materials through case studies, problem solving and discussion has been developed. In conclusion, introducing the active learning to the students on solving any problems improve the students’ ability in achieving the course outcome and thus the programme outcome of the Department of Environmental Engineering, Curtin University Malaysia.DOI: doi.org/10.24071/ijiet.2019.030207
Profiling of Seawater Bacterial Diversity in Tanjung Mas Port Using 16S rRNA eDNA Metabarcoding and Next-Generation Sequencing (NGS) Anggoro, Naufal Sebastian; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto; Wijanarka, Wijanarka; Zainuri, Muhammad; Hadibarata, Tony
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 1 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.1.61-70

Abstract

Tanjung Emas Port is the entry and exit point for trade commodities, both regional and international filled with many ships. This condition makes biofouling a very massive process in that place by various types of marine bacteria. The initial formation of a biofilm is relevant to bacterial diversity, colonization and adhesion. The objective of the study was assessing bacterial diversity in relation to with biofouling within Tanjung Mas Port Semarang, by using 16S rRNA eDNA metabarcoding Next-Generation Sequencing (NGS). Seawater samples from aquatic sites of Tanjung Mas harbor was used for DNA extraction and amplification of the 16S rRNA V-3-V4 hypervariable region, followed by sequencing and library construction of eDNA Metabarcoding. Sequence processing and analysis was performed in QIIME 2 and RStudio using DADA2 for advanced sequencing processing and Phyloseq. The results of this research showed that bacteria is the predominant taxon constituting 100% of the community. The taxon consists of Proteobacteria (49.38%), Bacteroidota (8.67%), and Firmicutes (8.88%). Alphaproteobacteria (20.92%) and Gammaproteobacteria (12.39%) dominate at the Class level, emphasizing their versatility and ecological influence. At the Order and Family levels reveals the prevalence of Rhodobacterales (10.04%), Chitinophagales (2.53%), Rhizobiales (3.61%), Rhodobacteraceae (5.67%), Saprospiraceae (3.63%), and Rhizobiaceae (2.15%). It was found that the unculturable taxa dominance in Tanjung Mas Port was 44.66%. These taxonomic entities contribute significantly to the taxonomic and functional diversity of the microbial community, influencing nutrient cycling, organic matter degradation, ecosystem stability and biofilm formation.
The Use of Scoring Rubrics in University Hadibarata, Tony; Hidayat, Topik; Kwabena, James
Acta Pedagogia Asiana Volume 3 - Issue 1 - 2024
Publisher : Tecno Scientifica Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53623/apga.v3i1.312

Abstract

This study examines the application of scoring rubrics as an evaluative instrument in higher education environments. Specifically, it explores the advantages, validity, and reliability associated with their use. Scoring rubrics provide a systematic and unbiased methodology for assessing various academic assignments, assisting educators in delivering constructive feedback and promoting student development. The study delves into the benefits associated with the utilization of rubrics, which encompass improved levels of transparency, uniformity, and fairness in the grading process. Furthermore, it investigates the validity and reliability of rubrics, ensuring the precision and consistency of evaluation outcomes. Moreover, this study explores the utilization of holistic and analytic rubrics within Malaysian universities, providing an analysis of their individual applications and advantages in the evaluation procedure. In the pursuit of improving assessment methods, institutions are increasingly recognizing the significance of including scoring rubrics as a valuable instrument to facilitate high-quality education and foster student achievement.
Bridging Theory and Practice: The Role of Site Visits in Environmental Engineering Learning Jusoh, Muhammad Noor Hazwan; Hadibarata, Tony
Acta Pedagogia Asiana Volume 3 - Issue 1 - 2024
Publisher : Tecno Scientifica Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53623/apga.v3i1.326

Abstract

In engineering education, experiential learning is essential as it allowed students to connect theoretical knowledge with real-world applications. This study explored the impact of industrial visits, a form of experiential learning, on engineering students’ comprehension, critical thinking, and overall educational experience. We employed a mixed-methods approach, integrating questionnaire surveys, reflective writing exercises, and qualitative analyses to assess the learning outcomes and pedagogical implications of these site visits. Our findings demonstrated that industrial visits significantly enhanced students’ understanding of engineering concepts by providing real-world context to theoretical knowledge. Through reflective exercises, students integrated their field trip experiences with classroom learning, fostering critical thinking abilities and a deeper comprehension of the subject matter. This study underscored the importance of curriculum integration. To nurture students as adept problem solvers and critical thinkers in both academic and practical engineering fields, fostering dynamic interactions between theoretical instruction and practical experiences was crucial. Our study encouraged further exploration of how experiential learning could enrich the educational journey, equipping students to tackle complex challenges in our ever-evolving environment as engineering education advanced.
Assessing Sustainability Literacy in Green School through Citizen Science Project on Biodiversity Learning Hamidah, Nur; Hidayat, Topik; Sriyati, Siti; Hadibarata, Tony
Jurnal Inovasi Pendidikan IPA Vol. 10 No. 2: Oktober 2024
Publisher : Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jipi.v10i2.71283

Abstract

The research aims to measure students' sustainability literacy after participating in a citizen science project. The subjects of this research were students at one of the state senior high schools (SMAN) in Bandung. The descriptive method with a quantitative approach was used in this research. The research was conducted over four weeks with a total of 12 hours of meetings in two classes with 72 students. Random sampling was used to establish the research sample. Data collection used assessment of students' sustainability literacy questions after they completed citizen science projects. Descriptive analysis was used to conclude the data. The finding shows that most students (93%) received passing grades above the minimum passing criteria (19.5% in sufficient, 55.5% good, and 18% excellent categories). It is shown that CSP-based learning is effective in strengthening the students' sustainability literacy.
Determination of chlorophyll-a and its distribution in the waters of the mangrove forest rehabilitation area in Mojo Estuaria, Pemalang Maharani, Galung Dhiva; Indarjo, Agus; Hadibarata, Tony; Zainuri, Muhammad; Endrawati, Hadi; Redjeki, Sri; Pribadi, Rudhi; Rochaddi, Baskoro; Ismanto, Aris; Jihadi, Muhammad Shulhan
Jurnal Kelautan Tropis Vol 27, No 2 (2024): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v27i2.23253

Abstract

Pemalang Regency has a mangrove area of around 2,839.44 but has been damaged reaching 453.38 Ha or about 16% of the existing area. This causes ecological functions in resisting abrasion, erosion, sedimentation, and carbon capture to decrease. Government efforts to restore the function of mangroves on the North Coast of Central Java carried out by rehabilitation require input from a water productivity approach. This study aims to determine the content and distribution of chlorophyll-a found in Pemalang Waters, Central Java. This was done by in situ observations in Pemalang Waters to see the fertility level of the waters, by purposive sampling at 20 sampling site, on April 30, 2024. The results obtained will be interpolated with ex situ data, including the Indonesian Earth Map (RBI), tidal data obtained from the SRGI website, flow data obtained from the Batnas website, and wind data obtained from the BMKG website. The next geospatial approach is ODV 4, Ms.Excel, and WR Plot application mapping; current analysis will be performed using ArcGIS 10.8 and Mike 21 applications; while the tidal analysis is carried out by the Admiralty method. The results of research conducted in Mojo Waters, Pemalang showed that the range of chlorophyll-a values was in the range of 1,858 - 41,287 mg / m3. The results were analyzed in sampling areas including rivers, estuaries, lagoons, eastern drift currents, western drift currents, waters still affected by estuaries, and the high seas. The distribution of chlorophyll values in these waters is the impact of nutrient supply from land because it is closely related to human activities, freshwater and seawater resuspension, drift currents, breaking waves, tidal currents. This causes the distribution pattern to occur from northeast to southwest.
Geospatial Modeling of the Nitrate Distribution as an Indicator of Aquatic Fertility in the Lagoon Waters of the Mangrove Information Center (PIM), Pekalongan Zainuri, Muhammad; Suryani, Oda Gracia Ariela; Ismanto, Aris; Handoyo, Gentur; Rifai, Azis; Rochaddi, Baskoro; Endrawati, Hadi; Kusumaningrum, Hermin Pancasakti; Jihadi, Muhammad Shulhan; Hadibarata, Tony
Jurnal Kelautan Tropis Vol 27, No 3 (2024): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v27i3.24081

Abstract

In the lagoon area of the Mangrove Information Center (PIM), Pekalongan, annual flooding leads to inundation in some land areas, particularly pond areas, lagoons, and residential areas. The area of submergence due to tidal floods in 2020 reached 783.99 hectares. The construction of embankments to the south of the lagoon as protection against flooding has transformed the northern area into a lagoon. Our research, crucial for understanding and managing these environmental changes, aims to determine and analyze the content and distribution of nitrate in the Lagoon Area of Mangrove Information Center Waters, Pekalongan. This was achieved through a thorough process of in situ observations based on purposive sampling at six sampling sites on June 22, 2023. The nitrate distribution data was then interpolated with a 2D current hydrodynamic model, ensuring the most accurate representation of the data. The value of nitrate content in the Mangrove Information Center lagoon ranges between 0.0065-0.1072 mg/L. This distribution pattern, influenced by deposits of mangrove litter, the depth of the lagoon, and water circulation, reflects the accumulation of physical and chemical influences in the waters.
Co-Authors Agus Indarjo AlMunqedhi, Bandar M. Ananda Putra, Gilang Anggoro, Naufal Sebastian Anto Budiharjo Aris Ismanto Aulia Permatasari, Berliana Azhary, Abrar Dalisfasha Azis Rifai Baskoro Rochaddi Bunrith, Seng Cao, Linh Thi Thuy Chen, Tse-Wei Chia, Xing Kai Citrasari, Nita Darwish, Noura M. Demir, Muslum Ersa, Ghina Rizqina Gani, Paran Gentur Handoyo Hadi Endrawati Hermin Pancasakti Kusumaningrum Hua, Ang Kean Jihadi, Muhammad Shulhan Jusoh , Mohamad Noor Hazwan Jusoh, Hisyam Jusoh, Muhammad Noor Hazwan Jusoh, Muhammad Noor Hisyam Khamidun, Mohd Hairul Kwabena, James Lai, Wai Yan Lestari, Diah Velentina Maharani, Galung Dhiva Malang, Jameson Matias-Peralta, Hazel Monica Muhammad Zainuri Musa, Muthah Najib, Mohamed Zuhaili Mohamed Nur Hamidah, Nur Ramadhany, Putri Risky Ayu Kristanti Rubiyatno Rudhi Pribadi Salman, Mohamad Sanro Tachibana Siti Sriyati Sri Redjeki Suryani, Oda Gracia Ariela Syafiuddin, Achmad Tang, Kuok Ho Daniel Tangahu, Bieby Voijant Teh, Zee Chuang Topik Hidayat Ujan, Nancy Umarie, Winda Voijant Tangahu, Bieby Wardhani, Widhowati Kesoema Wijanarka Wijanarka Yap, Chi Nam Yong, Leong Kong Yulisa, Arma Zaidi, Nur Syamimi Zhu Hang, Goh