Claim Missing Document
Check
Articles

Found 2 Documents
Search

Toxic compounds of Curcuma aeruginosa causes necrosis of mice hepatocytes Eka Pramyrtha Hestianah; Idha Kusumawati; Lucia Tri Suwanti; Sri Subekti
Universa Medicina Vol. 33 No. 2 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.118-125

Abstract

BACKGROUNDPeople have been using Curcuma aeruginosa rhizome as a traditional herbal medicine as appetite stimulant, without realizing its side effects. Herbal plants contain tens to hundreds of compounds, some of which are toxic. The aim of this research was to determine which toxic compound of Curcuma aeruginosa rhizome has an impact on apoptosis and PARP-1 expression of hepatocytes in male mice.METHODSEighty eight male Balb C mice were divided into 10 groups treated respectively with Curcuma aeruginosa rhizome cloroform extract, methanol extract, essential oil, infusion, and press juice, at dosages of 0.004g/kgBW and 0.06g/kgBW, and 1 control group. The treatment was given orally once a day for 10 days and on the 11th day, the research animals were sacrificed, and their liver taken for histopathologic slide preparation with Apopteq Detection Kit, and immunofluorescence. Compounds in Curcuma aeruginosa rhizome were analyzed with gas chromatography/mass spectrometry. The data obtained were analyzed by one-way ANOVA, and Partial Least Squares to determine which compounds had an impact on murine hepatocytes.RESULTSThe result of one way ANOVA showed that the chloroform groups at dosages of 0.004g/kgBW and 0.06g/kgBW showed the highest apoptosis of mice’s hepatocytes (p<0.05). There were significant differences in PARP-1 expression between control and treatment groups. The highest PARP-1 expression was in the essential oil group at a dosage of 0.06g/kg BW (p<0.05).CONCLUSIONCurcuma aeruginosa rhizome given to mice orally causes necrosis of mice’s hepatocytes.
PELATIHAN ON-LINE PEMBUATAN PERANGKAT HAND SANITIZER BERBAHAN NON-ALKOHOL PADA PEMUDA BINAAN Mochamad Lazuardi; Hani Plumeriastuti; Eka Pramyrtha Hestianah
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 2 (2020): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v4i2.2020.377-384

Abstract

Training on non-alcohol based hand sanitizer (HS) was conducted for assisted youth in five districts, namely Gresik, Bojonegoro, Lamongan, Probolinggo and Nganjuk for three days. In the training, competency strengthening was carried out, namely theory and practicum, ending with evaluation of activities. During the implementation of theory and practicum, training materials were provided using social media facilities in the form of WhatsApp and e-mail. Specifically for coordination with participants, a training group was created using social media. The results of the training evaluation turned out that in terms of strengthening the competence of knowledge in making HS and working mechanisms of HS, it was increasing. In terms of straightening the meaning of HS production to be more focused according to the basis of science or good manufacturing practices (GMP). Thus, youth assisted with criteria include youth who are able to pass on knowledge to all young people in their respective districts. Furthermore, the knowledge and skills of the assisted youth in making HS based on non-alcoholic substances will be more agile and targeted. Thus, in reality, the training on making non-alcohol based HS has made the assisted youths become pioneers at the end of the line to pass on their knowledge. The conclusion in the implementation of this training is that the assisted youth are capable and ready to make non-alcohol-based HS as well as being ready to produce for a small scale while showing real work to be transmitted (98%). abstrakTelah dilakukan pelatihan pembuatan hand sanitizer (HS) berbasis non-alkohol terhadap pemuda binaan di lima kabupaten yaitu Gresik, Bojonegoro, Lamongan, Probolinggo dan Nganjuk selama tiga hari. Dalam Pelatihan tersebut dilakukan pemberian penguatan kompetensi yaitu teori dan praktikum diakhiri dengan evaluasi kegiatan. Selama berlangsung pelaksanaan teori dan praktikum, materi pelatihan diberikan menggunakan fasilitas media sosial berupa whatsapp dan e-mail. Khusus koordinasi dengan peserta dilakukan pembuatan grup pelatihan menggunakan media sosial. Hasil evaluasi pelatihan ternyata dari segi penguatan kompetensi pengetahuan pembuatan HS dan mekanisme kerja HS, makin meningkat. Segi pelurusan pengertian produksi HS menjadi makin terarah sesuai basis ilmu pengetahuan atau cara pembuatan obat yang baik (CPOB). Dengan demikian pemuda binaan dengan kritetria termasuk pemuda yang sanggup menularkan ilmu ke seluruh masyarakat muda di kabupaten masing-masing. Lebihlanjut pengetahuan dan ketrampilan pemuda binaan dalam membuat HS berbasis bahan non-alkohol makin cekatan dan terarah. Dengan demikian seacara nyata pelatihan pembuatan HS berbasis non-alkohol telah menjadikan pemuda binaan sebagai pelopor di ujung garis untuk menularkan ilmunya. Kesimpulan dalam pelaksanaan pelatihan ini adalah para pemuda binaan telah sanggup dan siap membuat HS berbasis non-alkohol sekaligus siap berproduksi untuk skala kecil sambil memperlihatkan karya nyata untuk ditularkan (98 %).