Leni Maylina
Departemen Klinik, Reproduksi Dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor

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Journal : Jurnal Ilmu Pertanian Indonesia

Respons Antibodi Anti ETEC K99 pada Induk Sapi Bunting Setelah Pemberian Vaksin Escherichia Coli Polivalen Anita Esfandiari; Sus Derthi Widhyari; Sri Murtini; Bayu Febram; Retno Wulansari; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 2 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to detect antibody (IgG) against enterotoxigenic Escherichia coli (ETEC)K99 in the blood of cows vaccinated by Escherichia coli polyvalent vaccine. Eight dry cows were injectedsubcutaneously by polyvalent Escherichia coli twice prior to parturition. Before vaccinated, the cows were givenimmunomodulator orally for 3 days. Blood samples were drawn from coccigeal vein prior to the 1st vaccination,two week following the 1st vaccination and at 1, 2, and 4 weeks after the 2nd vaccination. Blood samples wereanalyzed for IgG and ETEC K99 using indirect Enzyme Linked Immunosorbent Assay (ELISA) assay. Results of theexperiment indicated that absorbance values of all vaccinated cows before the first vaccination until third weekfollowing the 2nd vaccination were below cut off values. The absorbance values then increased and were above cutoff values at fourth week following the 2nd vaccination. In conclusion, antibody against ETEC K99, were detected inthe blood of cows, fourth week following the 2nd vaccination.
Efek Penambahan Mineral Zn Terhadap Gambaran Hematologi pada Anak Sapi Frisian Holstein Sus Derthi Widhyari; Anita Esfandiari; Agus Wijaya; Retno Wulansari; Setyo Widodo; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to determine the effects of zinc supplementation on health status in dairy calves. Nine Frisian Holstein (FH) at 6-10 months of age were used in this experiment and divided into three groups. First group (no added Zn) for control, the second group was added 60 ppm Zn, and the third group was added 120 ppm Zn. Zn was administered daily for three months. Blood samples were collected from the jugular vein and anticoagulated with EDTA. Whole blood were used for measuring erythrocytes, hemoglobin concentration, hematocrit value, total leukocyte count, and leukocyte cell types. The results showed that no difference among groups for hematological parameters and the value of hematology were in the range values references. In conclusion, 60 and 120 ppm Zn supplementation in the feed is relatively safe for health.
Tinjauan Penambahan Mineral Zn dalam Pakan Terhadap Kualitas Spermatozoa pada Sapi Frisian holstein Jantan Sus Derthi Widhyari; Anita Esfandiari; Agus Wijaya; Retno Wulansari; Setyo Widodo; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 1 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Bulls are expected to be able to produce a good quality and quantity of sperm. The objective of this experiment was to study the effect of Zn supplementation on the sperm quality, in Frisian holstein bulls. Ten bulls, 1618 months of age were used in this experiment. The experimental bulls were divided into two groups, i.e.,group without Zn supplementation (control) and group with 60 ppm of Zn supplementation. Zn supplementations were given everyday for a period of four months. Semen was collected by using artificial vaginaat the end of the experiment. Semen quality was evaluated macroscopically and microscopically. The variables measured were semen volume, semen pH, sperm motility, sperm concentration, sperm viability, and sperm abnormality. The results showed that Zn supplementation significantly increased sperm motility and sperm concentration (P<0.05), whereas there was no significant difference in other parameters.