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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu Ternak Jurnal Medik Veteriner ARSHI Veterinary Letters Jurnal Kedokteran Hewan Asian Journal of Social and Humanities
Anita Esfandiari
IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Veterinary

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Profil protein total, albumin dan globulin pada ayam broiler yang diberi kungiy, bawang putih dan Zinc (Zn) Sus Derthi Widhyari; Anita Esfandiari; . Herlina
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to study the effectiveness of turmeric, garlic and zinc supplementation on protein, albumin and globulin concentration of broiler. One hundred DOC were divided into five treatments, four replications, consist of five chicks in each replicate. The treatments were RO (basal diet as a control), R1 (RO + 1,5% turmeric powder +2,5 % garlic powder), R2 (RO + 2,5% garlic powder + 120 ppm zinc), R3 (RO +1,5% turmeric powder+ 120ppm zinc) and R4 (RO +1,5 turmeric powder+ 2,5% garlic powder + 120 ppm zinc). The diet contain 23,5% crude protein and 3215 kcal metabolizable energy. Blood samples were taken from axillary veins at the three and six weeks of age. The results showed that total protein and globulin concentration at 6 weeks slightly higher than 3 weeks old chicks but not significantly different (P>0.05). Albumin concentration were highest on R3 treatment. Total protein and globulin concentration washighest on the R2 treatment. In conclusion, the supplementation of garlic (2.5%) and ZnO (120 ppm) showed the best combination to improve immune response in broiler.
Diare pada sapi neonatus yang ditantang Escherichia coli K-99 Anita Esfandiari; Sus Derthi Widhyari; Ali Hujarat
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to study the efficacy- of colostrum given to neonatal calves challenged by Escherichia coli (E. col!) K-99. Ten healthy calves devided into two groups i.e. colostrum group (given colostrum originated from cows vaccinated by E. coli) and non-colostrum group (given whole cow milk). Colostrum or milk were given to the calves at amounts of 10% of body weight directly after birth and Followed by the same amount every 12 h, for three days. Challenged were done orally to all newborn calves when they were 12 hours of ages, using live bacteria of E. coli K-99. Fecal samples were collected every 12 hours for one week. Results of the experiment indicated that all calves experienced diarrhea following challenge tests. However, the non colostrum group showed a frequent defecation, more liquid faeces, a longer length of diarrhea and more severe clinical signs of diarrhea. It was confirmed that E' coli found and every feacal Samples,collected. In conclusion, colostrums collected from cow vaccinated by E. coli showed protective properties against E. coli K-99 infection.
Respons Antibodi Anti ETEC K99 pada Induk Sapi Bunting Setelah Pemberian Vaksin Escherichia Coli Polivalen Anita Esfandiari; Sus Derthi Widhyari; Sri Murtini; Bayu Febram; Retno Wulansari; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 2 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to detect antibody (IgG) against enterotoxigenic Escherichia coli (ETEC)K99 in the blood of cows vaccinated by Escherichia coli polyvalent vaccine. Eight dry cows were injectedsubcutaneously by polyvalent Escherichia coli twice prior to parturition. Before vaccinated, the cows were givenimmunomodulator orally for 3 days. Blood samples were drawn from coccigeal vein prior to the 1st vaccination,two week following the 1st vaccination and at 1, 2, and 4 weeks after the 2nd vaccination. Blood samples wereanalyzed for IgG and ETEC K99 using indirect Enzyme Linked Immunosorbent Assay (ELISA) assay. Results of theexperiment indicated that absorbance values of all vaccinated cows before the first vaccination until third weekfollowing the 2nd vaccination were below cut off values. The absorbance values then increased and were above cutoff values at fourth week following the 2nd vaccination. In conclusion, antibody against ETEC K99, were detected inthe blood of cows, fourth week following the 2nd vaccination.
Efek Penambahan Mineral Zn Terhadap Gambaran Hematologi pada Anak Sapi Frisian Holstein Sus Derthi Widhyari; Anita Esfandiari; Agus Wijaya; Retno Wulansari; Setyo Widodo; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to determine the effects of zinc supplementation on health status in dairy calves. Nine Frisian Holstein (FH) at 6-10 months of age were used in this experiment and divided into three groups. First group (no added Zn) for control, the second group was added 60 ppm Zn, and the third group was added 120 ppm Zn. Zn was administered daily for three months. Blood samples were collected from the jugular vein and anticoagulated with EDTA. Whole blood were used for measuring erythrocytes, hemoglobin concentration, hematocrit value, total leukocyte count, and leukocyte cell types. The results showed that no difference among groups for hematological parameters and the value of hematology were in the range values references. In conclusion, 60 and 120 ppm Zn supplementation in the feed is relatively safe for health.
Tinjauan Penambahan Mineral Zn dalam Pakan Terhadap Kualitas Spermatozoa pada Sapi Frisian holstein Jantan Sus Derthi Widhyari; Anita Esfandiari; Agus Wijaya; Retno Wulansari; Setyo Widodo; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 1 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Bulls are expected to be able to produce a good quality and quantity of sperm. The objective of this experiment was to study the effect of Zn supplementation on the sperm quality, in Frisian holstein bulls. Ten bulls, 1618 months of age were used in this experiment. The experimental bulls were divided into two groups, i.e.,group without Zn supplementation (control) and group with 60 ppm of Zn supplementation. Zn supplementations were given everyday for a period of four months. Semen was collected by using artificial vaginaat the end of the experiment. Semen quality was evaluated macroscopically and microscopically. The variables measured were semen volume, semen pH, sperm motility, sperm concentration, sperm viability, and sperm abnormality. The results showed that Zn supplementation significantly increased sperm motility and sperm concentration (P<0.05), whereas there was no significant difference in other parameters.
Profil Kreatinin dan Nitrogen Urea Darah pada Anak Sapi Friesian Holstein yang Disuplementasi Zn Sus Derthi Widhyari; Anita Esfandiari; Aditia Dwi Cahyono
Acta VETERINARIA Indonesiana Vol. 3 No. 2 (2015): Juli 2015
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.41 KB) | DOI: 10.29244/avi.3.2.45-50

Abstract

Parameter kreatinin dan nitrogen urea darah atau blood urea nitrogen (BUN) dapat digunakan sebagai indikator untuk melihat adanya gangguan fungsi ginjal. Penelitian ini bertujuan untuk mengetahui kadar kreatinin dan BUN dalam serum anak sapi Friesian Holstein (FH) yang diberi suplementasi mineral Zn di dalam pakan. Penelitian ini menggunakan 9 ekor anak sapi FH yang sehat secara klinis dan umur berkisar antara 6-10 bulan. Hewan dibagi menjadi 3 kelompok, yaitu kelompok tanpa suplementasi Zn, kelompok yang diberi suplementasi Zn sebesar 60 ppm, dan kelompok yang diberi suplementasi Zn sebesar 120 ppm. Pengambilan sampel darah dilakukan melalui vena jugularis sebelum pemberian Zn dan setiap bulan setelah pemberian Zn selama 3 bulan. Sampel darah dalam bentuk serum dianalisis untuk kadar kreatinin dan BUN menggunakan alat spektrofotometer. Hasil pemeriksaan memperlihatkan bahwa kadar kreatinin darah berkisar antara 0,64 sampai 0,77 mg/dL, dan kadar BUN berkisar antara 8 sampai 19 mg/dL. Kadar kreatinin dan BUN darah pada anak sapi FH masih berada pada kisaran normal. Oleh karena itu suplementasi Zn 60 ppm maupun 120 ppm yang diberikan selama tiga bulan pada anak sapi FH relatif aman dan tidak menggangu fungsi ginjal.Kata kunci: mineral Zn, BUN, kreatinin, anak sapi Friesian Holstein. (Creatinin and Blood Urea Nitrogen Profiles on Friesian Holstein Calves Supplemented by Zn)Creatinine and blood urea nitrogen (BUN) can be used for indicator renal disfuction. The objective of this experiment was to study the concentration of creatinine and blood urea nitrogen in Friesian Holstein (FH) calves, received feed supplemented by Zn. Nine healthy Holstein calves, 6-10 months old were used in this experiment. The calves were devided into 3 groups, consisted of three calves, i.e. no supplementation (control), 60 ppm and 120 ppm of Zn supplementation. Blood sample were collected from jugular vein, before dan after supllementation Zn every month untill 3 months. Blood urea nitrogen and creatinine concentrations in serum were analysed using spectrophotometer. Results of the experiment had indicated that the creatinine and BUN concentration ranging between 0,64-0,77 mg/dL and 8-19 mg/dL, respectively. In conclusion, concentration of creatinine and Blood urea nitrogen were in a normal range. So the supplementation of Zn 60 and 120 ppm were given for three months in Friesian Holstein calves relatively safe for renal function.Keywords: zinc, blood urea nitrogen, creatinine, Friesian Holstein calves.
Kinerja Kesehatan Sapi Neonatus yang Diberi Kolostrum dari Induk Sapi yang Divaksin Escherichia coli Retno Wulansari; Anita Esfandiari; I Wayan Teguh Wibawan; Sri Murtini
Acta VETERINARIA Indonesiana Vol. 4 No. 1 (2016): Januari 2016
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.496 KB) | DOI: 10.29244/avi.4.1.19-26

Abstract

Penelitian ini dilakukan untuk mempelajari status kesehatan anak sapi baru lahir yang diberi kolostrum berasal dari induk yang telah divaksinasi dangan vaksin E. coli in-aktif. Sebelas ekor anak sapi baru lahir digunakan dalam penelitian ini dibagi menjadi dua kelompok yaitu kelompok kolostrum (diberi kolostrum sapi) dan non-kolostrum (diberi susu sapi). Kolostrum atau susu sapi diberikan sebanyak 10% berat badan secara langsung setelah dilahirkan dan selanjutnya tiap 12 jam selama 3 hari. Uji tantang dilakukan dengan pemberian E. Coli K99 hidup peroral saat anak sapi berumur 14 jam. Pemeriksaan status kesehatan termasuk suhu tubuh, frekuensi pulsus dan napas serta kualitas defikasi pada 0,12, 24, 48, 72 dan 168 jam setelah uji tantang. Hasil penelitian ini memperlihatkan bahwa diarrhea timbul pada 12 sampai 26 jam setelah uji tantang pada semua anak sapi kelompok non-kolostrun dan beberapa anak sapi kelompok kolostrum. Kelompok non-kolostrum memperlihatkan tanda klinis dengan diarrhe parah sebagai diarrhe profus yang berwarna pucat kekuningan. Bahkan 1 ekor mati pada 3 hari setelah uji tantang. Sebaliknya pada kelompok kolostrum hanya memperlihatkan diarrhe sedang pada 5 dari 8 ekor. Kesimpulan dalam penelitian ini adalah konsumsi kolostrum yang berasal dari induk yang telah divaksinasi dengan E. Coli memperlihatkan sifat protektif terhadap infeksi E. coli K99.Kata kunci: Escherichia coli, kolostrum, anak sapi baru lahir. (The Health Performance of Neonatal Calves Received Colostrum from Cows Vaccinated by Escherichia coli)This experiment was conducted to study the health status of neonatal calves received colostrum from cows vaccinated by in-active E. coli. Eleven healthy newborn calves were used in this experiment and divided into two groups, i.e colostrum (received bovine colostrum) and non-colostrum group (received bovine milk). Colostrum or milk were given to the calves at 10% of body weight directly after birth and followed every 12 h, for three days. Challenges were done orally to all newborn calves when they were 14 hours of ages, used live E. coli K-99. Examination of health status included body temperature, pulses and respiration rates, and defecations quality at 0, 12, 24, 48, 72 and 168 hours after challenges. Results of the experiment showed that diarrhea appeared at 12-26 hours after challenges in all calves of non-colostrum group and part of calves in colostrum group. The non-colostrum group showed a severe clinical signs of diarrhea as watery profuse diarrhea with pale yellowish color. One calf even death at three days after challenges. In contrast, the colostrum group showed only a mild diarrhea in 5 out of 8 calves. In conclusion, the consumption of colostrum originated from cows vaccinated by E. coli showed protective properties against E. coli K-99 infection.Keywords: Escherichia coli, colostrum, neonatal calves
PF-4 Immune Response of Dry Holstein Vaccinated by Killed Avian Influenza H5N1 Vaccine Anita Esfandiari; Sri Murtini; Sus Derthi Widhyari; Retno Wulansari; Bayu Febram; Leni Maylina; Arief Purwo Mihardi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

The establishment and spread of highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype in birds and coincident infections in humans since 2003 have raised concerns that we may be facing an influenza pandemic caused by an H5N1 influenza virus [4]. Globally, from January 2003 to 2 March 2017, there were 860 cases of human infection with avian influenza A(H5N1) virus reported from 16 countries worldwide. Of these 860 cases, 454 were fatal (CFR of 53%). The last case was reported from Indonesia on 26 September 2017 [7]. Although the human cases of H5N1 in Indonesia has decreased significantly since 2010, according to WHO until 2017 there have been reported 200 cases H5N1 in  human with 168 cases of deaths. The last case was reported from Indonesia on 26 September 2017. This is indicate Indonesia as the highest fatal case of H5N1 globally. With continued incidence of avian influenza due to existing AI H5N1 viruses in poultry, the avian influenza H5N1 has been believed remain to threaten Indonesia [6].Passive immunization using specific antibody against AI H5N1 from bovine colostrum is one of an alternative to control H5N1 virus infection due to lack of H5N1 vaccine production for human. Bovine colostrums consider an ideal alternative antibody source, as the antibody in the bovine’s blood is transported to mammary gland easily and accumulates in the colostrum in large quantities. As a “biological factory”  and the source of natural antibody, bovine colostrum could be designed to produce the specific antibody against certain disease for animal and human by immunizing the dry cow (with the antigen of interest).Vaccine is an antigenic material used to produce active immunity against diseases. Vaccination is the administration of vaccine to the individual to generate immunity against a disease [2]. According to [1], the exposure of a dry cow against antigen (vaccine) will produce specific antibody in their blood circulation. Therefore, evaluation on the status of antibody against AI H5N1 following the vaccination of dry Holstein cows with killed AI H5N1 vaccine is needed. 
KIVFA-2 Efek Imunomodulator terhadap Profil Leukosit Induk Sapi Friesian Holstein yang Diberi Antigen AI H5N1 Inaktif Sri Murtini; Anita Esfandiari; Sus Derthi Widhyarti; Retno Wulansari; Leni Maylina; Arif Purwo Mihardi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Immunomodulator adalah zat yang memiliki kemampuan untuk meningkatkan  atau menekan respon imun. Cox (1988) melaporkan bahwa pengaruhnya  selain terhadap  respon imun, imunomodulator juga dapat memodulasi haematopoiesis, termasuk peningkatan jumlah RBC dan WBC (leukosit), peningkatan PCV dan aktivasi makrofag.  Sapi friesian holstein (FH) merupakan sapi perah yang dapat digunakan sebagai hewan donor penghasil immunoglobulin G anti AI H5N1 melalui produk kolostrumnya (Esfandiari et al, 2007).  Guna meningkatkan titer immunoglobulin pada hewan donor hiperimun sera umumnya hewan diberi imunomdulator.  Berbagai jenis bahan seperti glucan, lectin, dan berbagai jenis polisakarida dari tanaman maupun hewan (Alamgir dan Uddin 2010) serta polipeptide ribonukleotida dapat digunakan sebagai imunomodulator.(Hess dan Greenberg 2012)  Penggunaan  polipeptida ribonukleotida sebagai imunomodulator ada sapi saat ini belum banyak diteliti.  Pemberian imunomodulator memberikan dampak perubahan gambaran leukosit secara langsung maupun tidak langsung.  Dampak pemberian imunomodulator jenis polipeptide ribonukleotida pada sapi FH bunting trimester terakhir untuk tujuan produksi hiperimunsera melalui produksi kolostrum belum pernah dilaporkan.  Penelitian ini bertujuan untuk mengetahui efek pemberian imunomodulator peptide ribonukleotida pada sapi FH yang disuntik antigen AI H5N1 inaktif.
KIVFA-8 Studi Kasus: Profil Mineral Makro Pada Sapi Perah Yang Mengalami Retensi Plasenta di Kunak Kabupaten Bogor Retno Wulansari; Anita Esfandiari; Sus Derthi Widhyarti; Chusnul Choliq; Arief Purwo Mihardi; Leni Maylina; . Suryono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Susu merupakan salah satu bahan pangan yang sangat penting bagi masyarakat untuk memenuhi kebutuhan gizinya. Peningkatan kesadaran masyarakat akan pentingnya susu sebagai salah satu sumber gizi yang penting berefek terhadap kebutuhan susu nasional terus meningkat pula. Namun kebutuhan yang tinggi ini belum diimbangi dengan produksi susu nasional yang baru mencapai 3.29% per tahun, sehingga kekurangan akan kebutuhan susu ini masih harus diimport dari negara lain. Rata-rata produksi susu di Indonesia dari setiap sapi masih relatif rendah, sekitar 10-12 liter/ekor/hari (Deny 2014). Keterbatasan produksi susu dari dalam negeri ini disebabkan oleh masih belum maksimalnya produksi susu dari setiap sapi perah yang dimiliki oleh peternak di Indonesia.Manajemen pemeliharaan yang baik dalam usaha peternakan sapi perah sangat diperlukan untuk dapat meningkatkan produksi susu, salah satunya antara lain manajemen pakan. Pakan harus memenuhi unsur-unsur penting diantaranya mineral makro dan mikro dalam jumlah yang secukupnya.Masalah kesehatan yang sering ditemui pada sapi perah awal laktasi umumnya berupa gangguan metabolik, seperti milk fever dan ketosis (Divers & Peek 2008). Hipokalsemia adalah kelainan metabolik dimana mekanisme homeostasis gagal untuk mempertahankan konsentrasi Ca darah normal saat awal laktasi (Chamberlain et al. 2013). Kejadian ini sering didahului dengan kondisi hipokalsemia subklinis pada saat bunting dan kering kandang, tetapi tidak teramati oleh peternak (Goff 2008). Retensi Plasenta merupakan salah satu manifestasi dari gangguan metabolik akibat tidak cukupnya konsentrasi mineral pada hewan post partus. Sapi secara normal akan melepaskan plasenta dalam waktu 3 -6 jam post partus. Retensi atau tertahannya plasenta lebih dari 8 – 12 jam pada induk post partus dapat dipertimbangkan sebagai suatu kondisi yang abnormal (Diver & Peek 2008. Salah satu predisposisi adalah tidak adanya program manajemen pakan atau pemberian suplemen mineral yang tidak tepat, sebagaimana disajikan pada studi kasus berikut.
Co-Authors . Herlina . Suryono Aditia Dwi Cahyono Agus Lelana Agus Setiyono Agus Wijaya Agus Wijaya Ali Hujarat Amrozi Arief Purwo Mihardi Arif Purwo Mihardi Arifianto, Dinar Azzahrah, Putri Bambang Pontjo Priosoeryanto Bayu Febram Bayu Firmala Kusuma Chusnul Choliq Chusnul Choliq Damiana Rita Ekastuti Darsono Darsono Deni Noviana Deny Setyo Wibowo Dewi, Fitriya Nur Annisa Dondin Sajuthi Dwi Utari Rahmiati Effendi, Siti Aisyah Rahmalia Erni Sulistiawati Fitri, Arni Diana Friska Mery Montolalu Gagah Hendra Wijaya Gunanti Gunanti . Henny Nuraini Huda Shalahudin Darusman I Ketut Sutama I wayan Teguh Wibawan Ida Zahidah Irfan IETJE WIENTARSIH Iman, Bintang Nurul Joan Elviyanti Joesoef, Jayusman Arsiyanti Kartjito, Nicolas Edward Christanto Kathirina Beatrik Riwu Wolo Khairita, Lia Kusumawati, Nina Tri Leni Maylina Lia Khairita Lina Noviyanti Sutardi Lingga Surya Maret Daulay Madyastuti, Rini Maharani Maharani, Maharani Maret Daulay, Lingga Surya Mohamad Yamin Nina Kusumawati Ningtias, Putri Indah Nuzul Asmilia Nuzul Asmilia Retno Wulansari Rizal Arifin Akbari Rizal Rahadian Ramdhany Robby Wienanto Robby Wienanto Safitria Wulandari Setiadi, Hirawan Setyo Widi Nugroho Setyo Widodo Setyo Widodo Soesatyoratih, Rr Solinda, Gamelita Rizkawandi sri murtini . Sri Wahyuni Subangkit, Mawar Suryo Saputro Sus Derthi Widhyai Sus Derthi Widhyari Sus Derthi Widhyarti Tiara Widyaputri Virgilius Martin Kelake Kedang Virgilius Martin Kelake Kedang Wasmen Manalu Wiwin Winarsih Wolo, Kathirina Beathrik Riwu