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All Journal ARSHI Veterinary Letters Gunung Djati Conference Series
Ita Krissanti
Universitas Padjadjaran
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Sintesis antigen AFB1-BSA dan konjugasi antibodi anti AFB1-BSA dengan nanopartikel emas sebagai pereaksi imunostrip Ita Krissanti; Agustin Indrawati; Romsyah Maryam
ARSHI Veterinary Letters Vol. 4 No. 4 (2020): ARSHI Veterinary Letters - November 2020
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.4.4.77-78

Abstract

Aflatoksin B1 (AFB1) often contaminates a great variety of foods and animal feeds that will be dangerous if con-sumed by humans or animals. Rapid detection techniques that can be used in the field is really needed to monitor AFB1 contamination. The aims of this study were to perform synthesis of AFB1-BSA antigen and conjugation of antibody against AFB1-BSA to gold nanoparticle as immunostrip-test reagents. The AFB1-CMO was identified on TLC and AFB1-BSA was characterized using SDS PAGE. The AFB1-CMO formation indicated as a blue spot at 0.45 retention factor (Rf) on TLC and the AFB1-BSA antigen revealed as a single band protein at about 72 kDa molecular weight on the SDS PAGE. Conjugation of antibody against AFB1-BSA to gold nanoparticle resulted in the formation of red-dish purple compound which can be used for the detection of AFB1 on immunostrip. The optimum composition achieved in concentration of AFB1-BSA 1-1.5 mg/ml, IgG anti rabbit 0.1 mg/ml, and antibody against AFB1-BSA-gold nanoparticle conjugate in 0.5x0.5 cm² area characterized by the establishment of two reddish purple lines in the test and control zone.
Efektivitas dan Pengaruh Kombinasi Anestesi Ketamine-Xylazine pada Tikus (Rattus norvegicus) Ita Krissanti; Rifaati Hanifa; Resti Gradia Dwiwina
Gunung Djati Conference Series Vol. 18 (2023): Prosiding Seminar Nasional Biologi (SEMABIO) Tahun 2022
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.581 KB)

Abstract

Anesthesia is used in several studies to reduce or eliminate pain and suffering in experimental animals. One of the most widely used experimental animal species in research is rat (Rattus norvegicus). Ketamine-xylazine (KX) is a combination that is often used for injection anesthesia in rats. The properties of the KX combination ideally can induce the desired effect, such as analgesics, muscle relaxation, and sedation. However, the KX combination can cause side effects that affects the physiological function in rat’s body. This literature study discusses the variety of ketamine-xylazine combination doses that can be used as anesthesia in rats, their effect on cardiorespiratory parameters and body temperature in rats. It can be concluded that the combination of ketamine-xylazine provides a variety of doses that produce a fast onset of action and a duration of action that can be adjusted according to needs.
Co-Authors Agustin Indrawati Resti Gradia Dwiwina Rifaati Hanifa Romsyah Maryam