Garuda - Garba Rujukan Digital

Tasliah Tasliah

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111

Author-ID : 401848

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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1

IMPROVEMENT OF EARLY MATURITY IN RICE VARIETY BY MARKER ASSISTED BACKCROSS BREEDING OF Hd2 GENE Fatimah, Fatimah; Prasetiyono, Joko; Dadang, Ahmad; Tasliah, Tasliah
Indonesian Journal of Agricultural Science Vol 15, No 2 (2014): October 2014
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Early-maturing and high-yielding rice variety is very useful for increasing rice production in Indonesia. The aim of this research was to develop new lines of Indonesian rice containing Hd2 gene using Code variety as a recipient parent and Nipponbare variety as a donor parent through targetted MAB approach using RM1362 and RM7601 in chromosom 7 for foreground selection. After two generations of backcrossing, the positive alleles of Hd2 gene from Nipponbare had successfully trans-ferred into Code. The plant number CdNp_29 in BC2F2 popula-tion had the highest genome recovery of 82.7%. The twelve BC2F3 plants were selected for self-pollination to generate BC2F4. These selected lines that carried the Hd2 gene were screened in the greenhouse for the evaluation of heading date and agronomic traits. All improved lines had Hd2 gene similar to the donor parent Nipponbare. The heading date of the breeding lines ranged from 73 to 89 days (Code 85 days) or fill the third criterion of rice maturity that is 103-104 days compared to Code of 116-119 days, whereas their agronomic performances were similar with that of Code. Application of MABc for im-proving rice early maturity has accelerated the development and selection in early generation of superior lines having genetic background of Code. It is expected that the newly developed lines of Code will be utilized to increase rice production in Indonesia.

KERAGAMAN BAKTERI ENDOFITIK DnSOLASI DARI EMPAT VARIETAS PADI DENGAN METODA ARDRA Susilowati, Dwi N; Hidayatun, Nurul; Tasliah, Tasliah; Mulya, K
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1978

Sixty eight endophytic bacteria were isolated from four different rice varieties (IR64, Cirata, Code and Limboto) obtained from agroecosystem in Cikembar, Sukabumi, West Java. Those isolates were subjected for analysis the diversity based on genetic fingerprinting through Amplified Ribosomal DNA Restriction Analysis (ARDRA) method. The objective of this research is to characterize the predominant endophyte bacteria are present within various rice varieties grown on agroecosystem in Cikembar, Sukabumi by using ARDRA method. The results shows that restriction analysis with both Rsal and HaelH was sufficient to allocate the endophyte bacteria from four different rice varieties into the 29 types. Moreover, Rsal alone was capable of resolving the 11 types, followed by HaelH 14 types. In general, the result may explain that there is no collinierity between the cluster and their host plant. Staphylococcus, Bacillus, Agrobacterium, Serratia, Klebsiella, Acidovorax and Pseudomonas were identified as endophytic bacteria from rice varieties in this agroecosystem based on their 16S rRNA sequences. Seven types were placed in close proximity to these genera, but other types were still unknown. Among these isolates, genera Staphylococcus and Bacillus are common to rice endophytes.

PERBAIKAN PADI (Oryza sativa L.) VARIETAS CIHERANG UNTUK SIFAT UMUR GENJAH DAN PRODUKSI TINGGI MENGGUNAKAN MARKA MOLEKULER Prasetiyono, Joko; Tasliah, Tasliah; Dadang, Ahmad; Fatimah, Fatimah
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The improvement of rice (Oryza sativa L.) Ciherang variety for early maturity and high production traits was carried out by Marker Assisted Backcrossing (MAB) method using Nipponbare as donor parent. The foreground selection of Hd2 gene was laid on flanking markers of RM1362 and RM7601 in QTL region. The selection process of F1 to BC2F2 plants were based on molecular markers and agronomic characters. The BC2F3 plants were challenged to bacterial leaf blight to know their resistance in this hybridization. The results indicated that the foreground and background selection were not sufficient as selection tools therefore they would be more accurate if assosiated with agronomic characters. Four selected lines derived from Ciherang x Nipponbare crossed (BC2F3 plant # 283, 307, 373, and 462) could be promising lines with early maturity and high productioncompared to Ciherang. Selected BC2F3 lines flowered earlier than original Ciherang up to 7-10 days, while the yield increasing was 3.55 to 9.2% higher based on weight of filled grains/plant, and from 3.58 to 19.39% higher based on the number of filled grain/plant. However, all of BC2F3 lines were not resistant to bacterial leaf blight attack.

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