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All Journal Vegetalika Planta Tropika BERITA BIOLOGI JURNAL BIOLOGI INDONESIA JURNAL PENDIDIKAN TAMBUSAI Jurnal Ilmiah Wahana Pendidikan
Nurul Hidayatun
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Ragunan 29, Pasar Minggu, Jakarta Selatan 12540, Indonesia
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Social Sciences Other

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Pengembangan Identitas Spesifik Berbasis Marka SSR pada 29 Varietas Kedelai Lokal Indonesia Lestari, Puji; Risliawati, Andari; Utami, Dwinita Wikan; Hidayatun, Nurul; Santoso, Tri Joko; Chaerani, Chaerani
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (765.015 KB) | DOI: 10.14203/jbi.v12i2.2888

Abstract

ABSTRACTSoybean is an important legume crop in Indonesia and local varieties from different regions are diversed. Accurate identification of local varieties is needed to determine the genetic identity in order to protect them to indicate the geographical origin and registration purpose. This study aimed to analyze DNA fingerprints of local varieties of soybean in Indonesia to develop a specific identity using SSR markers along with the marker set. A total of 29 local varieties/accessions was analyzed using 9 selected SSR markers with semi automated genetic analyzer on the basis of capillary electrophoresis. Total markers successfully detected 50 alleles in all varieties, with the average polymorphism information content (PIC) of each locus (0.579) and the genetic diversity index (0.629) were quite high as a reflection of diversity of the local varieties. Based on the analysis of the genetic diversity index, PIC, rare alleles, dominant allele frequency, analysis of genetic distance, 6 SSRs (Satt009, Satt038, Satt177, Satt242, Satt308 and Satt114) were selected as a set of markers for variety identification. Although three varieties can not be distinguished because of their close genetic relationship, but the digital coding indicates that local varieties of soybeans have possessed specific identities (ID) representing variation of SSR alleles. The selected SSR loci with additional identifier can be used to develop ID of Indonesian local soybean in the germplasm collection. Overall, the marker set is a powerful tool to complement morphological markers for the protection of local soybean varieties that contribute in accelerating local varieties improvement in the future agriculture.Keywords: specific identity, SSR, Indonesian local soybean variety, DNA fingerprint.
KERAGAMAN BAKTERI ENDOFITIK DnSOLASI DARI EMPAT VARIETAS PADI DENGAN METODA ARDRA Susilowati, Dwi N; Hidayatun, Nurul; Tasliah, Tasliah; Mulya, K
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1978

Abstract

Sixty eight endophytic bacteria were isolated from four different rice varieties (IR64, Cirata, Code and Limboto) obtained from agroecosystem in Cikembar, Sukabumi, West Java. Those isolates were subjected for analysis the diversity based on genetic fingerprinting through Amplified Ribosomal DNA Restriction Analysis (ARDRA) method. The objective of this research is to characterize the predominant endophyte bacteria are present within various rice varieties grown on agroecosystem in Cikembar, Sukabumi by using ARDRA method. The results shows that restriction analysis with both Rsal and HaelH was sufficient to allocate the endophyte bacteria from four different rice varieties into the 29 types. Moreover, Rsal alone was capable of resolving the 11 types, followed by HaelH 14 types. In general, the result may explain that there is no collinierity between the cluster and their host plant. Staphylococcus, Bacillus, Agrobacterium, Serratia, Klebsiella, Acidovorax and Pseudomonas were identified as endophytic bacteria from rice varieties in this agroecosystem based on their 16S rRNA sequences. Seven types were placed in close proximity to these genera, but other types were still unknown. Among these isolates, genera Staphylococcus and Bacillus are common to rice endophytes.
IDENTIFIKASI 26 ISOLAT BAKTERI ENDOFITIK DAN FILOSFER PADI DENGAN ANALISIS SEKUEN16S RDNA Hidayatun, Nurul; Susilowati, Dwi N; K Mulya, K Mulya
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.127 KB) | DOI: 10.14203/beritabiologi.v10i4.763

Abstract

The research was subjected to identify 26 endophytic and phyllosphere bacteria isolated from 4 rice varieties using sequencing method of 16S rDNA.The sequences were then aligned with reference sequence from Gen-Bank data library by BLAST program from NCBI to find the most related identity of the isolates analysed.The sequencing analysis revealed that some isolates have a high identity to Staphylococcus and Serratia (5 isolates each), Bacillus (4 isolates), Microbacteria (3 isolates), Pseudomonas (2isolates) and Klebsiella, Acidovorak, Bulkholderiaceae, Agrobacterium and Shewanella (1 isolate each). No specific isolate dominated in both endophytic and phyllosphere group, nor host-specific isolate found in the four rice varieties.
PENGEMBANGAN IDENTITAS SPESIFIK BERBASIS MARKA SSR PADA 29 VARIETAS KEDELAI LOKAL INDONESIA Lestari, Puji; Risliawati, Andari; Utami, Dwinita Wikan; Hidayatun, Nurul; Santoso, Tri Joko; Chaerani, Chaerani
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2888

Abstract

ABSTRACTSoybean is an important legume crop in Indonesia and local varieties from different regions are diversed. Accurate identification of local varieties is needed to determine the genetic identity in order to protect them to indicate the geographical origin and registration purpose. This study aimed to analyze DNA fingerprints of local varieties of soybean in Indonesia to develop a specific identity using SSR markers along with the marker set. A total of 29 local varieties/accessions was analyzed using 9 selected SSR markers with semi automated genetic analyzer on the basis of capillary electrophoresis. Total markers successfully detected 50 alleles in all varieties, with the average polymorphism information content (PIC) of each locus (0.579) and the genetic diversity index (0.629) were quite high as a reflection of diversity of the local varieties. Based on the analysis of the genetic diversity index, PIC, rare alleles, dominant allele frequency, analysis of genetic distance, 6 SSRs (Satt009, Satt038, Satt177, Satt242, Satt308 and Satt114) were selected as a set of markers for variety identification. Although three varieties can not be distinguished because of their close genetic relationship, but the digital coding indicates that local varieties of soybeans have possessed specific identities (ID) representing variation of SSR alleles. The selected SSR loci with additional identifier can be used to develop ID of Indonesian local soybean in the germplasm collection. Overall, the marker set is a powerful tool to complement morphological markers for the protection of local soybean varieties that contribute in accelerating local varieties improvement in the future agriculture.Keywords: specific identity, SSR, Indonesian local soybean variety, DNA fingerprint.
Evaluasi Karakter Morfo-fisiologis Sumber Daya Genetik Padi Berumur Genjah Setyowati, Mamik; Hidayatun, Nurul; Sutoro, Sutoro; Kurniawan, Hakim
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 2, No 2 (2014)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2014.025.66-73

Abstract

Rice varieties with short maturity and high productivity can be achieved when there is available genetic resources in a sufficient level of variability. Morphological characters, such as leaf thickness and crop growth rate, could affect in crop productivity. Evaluation of seed germination rate, crop growth rate,  and their efficiency used for nitrogen have been done on 25 accessions of rice. Seed germination rate of B11283-6C-PN-5-MR-34-1-1-3, Lariang, B11742-RS-2-3-MR-34-1-2-3 reached 1,0-1,3 g/hill/day during seed initiation, while Dodokan and Silugonggo reached 0.7-0.8 g/hill/day. Danau Atas, B.11283-6C-PN-5-MR-2-3-Sl-1-2-1-1, B11742-RS-2-3-MR-34-1-2-3 and B11742-RS-2-3-MR-34-1-4-1 lines show good perfomance, moreover B11742-RS-2-3-MR-34-1-1-3, B11742-RS-2-3-MR-34-1-1-4 and B11742-RS-2-3-MR-34-1-1-4 lines produced grain yield higher than Silugonggo and Dodokan varieties. Those lines could be harvested at about 96 days after planting.
Tinjauan Kuantitatif Tentang Faktor-Faktor Kepemimpinan Yang Mempengaruhi Kinerja Organisasi Rizqi, Muhammad; Heryanti, Andini Dwi; Said, Yasir; Hidayatun, Nurul; Wijaya, Muhammad Adhimawan
Jurnal Ilmiah Wahana Pendidikan Vol 11 No 2.D (2025): Jurnal Ilmiah Wahana Pendidikan 
Publisher : Peneliti.net

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to quantitatively evaluate the leadership factors that influence organizational performance. Through statistical approaches and data analysis, the study identifies key variables related to leadership effectiveness and how these variables impact organizational performance. The factors analyzed include leadership styles, communication, decision-making, motivation, and team development. Data were collected through surveys from various organizations in the industrial and service sectors. The results show that transactional and transformational leadership styles have a significant influence on organizational performance. Additionally, effective communication and participative decision-making were found to be major determinants of high performance. This study provides valuable insights for management practitioners in improving organizational performance through the development of more effective leadership, focusing on enhancing communication and employee involvement in decision-making processes.
Implementasi Disiplin Kerja Sebagai Upaya Mewujudkan Tempat Kerja yang Nyaman dan Efisien di CV. XYZ Hidayatun, Nurul; Isyanto, Puji
Jurnal Pendidikan Tambusai Vol. 9 No. 2 (2025): Agustus
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v9i2.30315

Abstract

Disiplin kerja merupakan salah satu pilar utama dalam pengelolaan sumber daya manusia yang efektif, terutama dalam menciptakan lingkungan kerja yang nyaman dan efisien. Di tengah dinamika dunia kerja yang semakin kompleks, banyak organisasi menghadapi tantangan dalam menegakkan disiplin karyawan secara konsisten. Penelitian ini bertujuan untuk mendeskripsikan implementasi disiplin kerja di CV. XYZ serta menilai kontribusinya terhadap terciptanya tempat kerja yang mendukung produktivitas dan kenyamanan kerja. Pendekatan yang digunakan adalah kualitatif deskriptif dengan teknik pengumpulan data melalui wawancara mendalam, observasi partisipatif, dan dokumentasi internal perusahaan. Informan penelitian dipilih secara purposif dari berbagai level organisasi, termasuk manajerial dan operasional. Hasil penelitian menunjukkan bahwa meskipun sistem disiplin telah diterapkan melalui kebijakan ketepatan waktu, kepatuhan terhadap SOP, dan tanggung jawab individu, pelaksanaannya belum seragam di seluruh bagian. Faktor kepemimpinan, komunikasi organisasi, dan pembinaan internal berperan besar dalam menentukan keberhasilan implementasi tersebut. Penelitian ini diharapkan dapat memberikan kontribusi teoritis terhadap pengembangan manajemen sumber daya manusia berbasis kedisiplinan, serta menjadi rujukan praktis dalam membentuk budaya kerja yang kondusif dan produktif di sektor industri menengah.
Kestabilan Karakter Fenotipik Plasma Nutfah Talas (Colocasia esculenta) Pasca Pemeliharaan pada Media Pertumbuhan Minimal dalam Kultur In vitro Sabda, Muhamad; Hidayatun, Nurul; Dewi, Nurwita
Vegetalika Vol 13, No 1 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.83669

Abstract

Talas (Colocasia esculenta) merupakan tanaman berumbi yang banyak dibudidayakan dan dimanfaatkan sebagai sumber pangan alternatif di Indonesia. Tanaman ini umumnya dikonservasi secara konvensional di lapang dan didukung dengan konservasi secara in vitro. Akan tetapi, konservasi secara in vitro rentan terhadap terjadinya perubahan genetik tanaman. Penelitian ini dimaksudkan untuk mengetahui daya tumbuh dan kestabilan karakter fenotipe aksesi plasma nutfah talas pasca pemeliharaan dalam pertumbuhan minimal dalam kultur in vitro. Sebanyak 12 aksesi talas yang telah dipelihara dalam media dengan penghambat pertumbuhan paclobutrazol diaklimatisasi dan ditumbuhkan di lapang. Delapan karakter fenotipe diamati dan dibandingkan antara aksesi dari kultur in vitro dengan aksesi dari lapang. Pengamatan mengacu pada Descriptor standard. Hasil penelitian menunjukkan bahwa tanaman yang dipelihara dalam kondisi pertumbuhan minimal dapat pulih daya tumbuhnya. Pemulihan daya tumbuh ini ditunjukkan dengan pertumbuhan yang normal di lapang tanpa mengalami perubahan karakter fenotipe. Hal ini menunjukkan bahwa penggunaan media pertumbuhan minimal yang terdiri dari MS + manitol 40 g/l dan MS + paclobutrazol 2 mg/l, tidak menimbulkan dampak yang buruk terhadap tanaman. Dengan demikian, media ini dapat direkomendasikan untuk konservasi in vitro plasma nutfah talas.
Co-Authors A.A. Ketut Agung Cahyawan W Andari Risliawati, Andari Chaerani Chaerani Dwi N Susilowati DWINITA WIKAN UTAMI Hakim Kurniawan, Hakim Heryanti, Andini Dwi Isyanto, H. Puji K Mulya, K Mulya Mamik Setyowati, Mamik Muhammad Adhimawan Wijaya Muhammad Rizqi Mulya, K Nurwita Dewi PUJI LESTARI Sabda, Muhamad Said, Yasir Sutoro Sutoro Tasliah Tasliah TRI JOKO SANTOSO