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All Journal Jurnal Matematika & Sains Tumbuhan : Publikasi Ilmu Sosiologi Pertanian dan Ilmu Kehutanan
Tatang Hernawan
Dept. of Pharmacy, Faculty of Mathematics and Natural Sciences, ITB
Agriculture, Biological Sciences & Forestry Mathematics

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Isolation and Purification of Dehalogenase From Pseudomonas cepacia UK7WS1 strain Tatang Hernawan; S. Soemitro S. Soemitro; H. Dewayani H. Dewayani; Tetih Tetih; W. T. Ismaya
Jurnal Matematika & Sains Vol 4, No 2 (1999)
Publisher : Institut Teknologi Bandung

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Abstract

Intracellular dehalogenase enzyme had been isolated from Pseudomonas cepacia UK7WS1 strain which was grown in MSM broth containing yeast extract (0.18 g/L) and MCPA (0,02 g/L) and incubated for 22-24 h at 30 oC. Enzyme purification was carried out by ammonium sulphate fractionation, DEAE-cellulose column chromatography and Sephadex G-75 gel filtration column chromatography. Protein purify factor achieved was 12.4 times and protein yield was 10 % w/w of the crude cell extract protein. The enzyme purity of each purification stage was monitored by SDS-polyacrylamide gel electrophoresis and resulted in one protein band for each denatured enzyme (dimeric protein of two identical subunits) and non-denatured enzyme (monomeric protein subunit).
Pengamatan Struktur Internal Organ Reproduksi Tumbuhan Gymnospermae dan Angiospermae pada Bunga Bakung (Hippeastrum sp.), Pinus (Pinus merkusii), serta Pengamatan Preparat Awetan Organ Reproduksi Jagung (Zea mays) Salma Humairo; Saskia Asila Zahwa; Tatang Hernawan; Tifani Siti Fatiah; Yasmin Pajrin Maulani; Ita Fitriyyah
Tumbuhan : Publikasi Ilmu Sosiologi Pertanian Dan Ilmu Kehutanan Vol. 2 No. 1 (2025): Januari : Tumbuhan : Publikasi Ilmu Sosiologi Pertanian Dan Ilmu Kehutanan
Publisher : Asosiasi Riset Ilmu Tanaman Dan Hewan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/tumbuhan.v2i1.192

Abstract

Seed plants or spermatophytes are divided into two, namely angiosperms (open seed plants) and gymnosperms (closed seed plants). These seeds are a means of generative development of spermatophytes. This research aims to observe the internal structure of the reproductive organs of seed plants and observe the phases of embryo development in seed plants.  The method used is observing the structure of internal plant organs and is supported by literature studies related to the research carried out. The objects of this research are lilies (Hippeastrum sp.), pine (Pinus merkusii), and preserved corn (Zea mays).
Co-Authors H. Dewayani H. Dewayani Ita Fitriyyah S. Soemitro S. Soemitro Salma Humairo Saskia Asila Zahwa Tetih Tetih Tifani Siti Fatiah W. T. Ismaya Yasmin Pajrin Maulani