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Effects of bovine seminal plasma on frozen semen quality of swamp buffaloes (Bubalus bubalis Amin, Muhammad Rizal; Toelihere, Mozes R; Yusuf, tuty L; Situmorang, Polmer
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 3 (1999)
Publisher : Indonesian Animal Sciences Society

Semen of two healthy swamp buffalo bulls were collected twice a week using artificial vagina. After initial evaluation, semen was divided into two parts, 1/3 for control (PK) and 2/3 for treatment (PS) and centrifugated at 3,000 rpm for 20 minutes. Seminal plasma of the second part was removed and changed with bovine seminal plasma for 0 minute (PS0) and 5 minutes (PS5) before semen was diluted with lactose extender containing 7% glycerol. Mean percentage of motility after thawing for PS5 (55.71%) was significantly higher (P<0.01) than PK (41.43%), but not significantly different with PS0 (52.43%). PS0 was significantly higher than PK. Mean percentage of live sperm and intact plasma membrane for PS5 (63.43% and 64.71%) were significantly higher (P<0.01) than PK (55.71% and 53.57%), but not significantly different with PS0 (61.14% and 59.28%). There was no significantly difference between PS0 and PK for mean percentage of live sperm and intact plasma membrane parameters. Mean percentage of intact acrosomal for PS5 (53.57%) was significantly higher (P<0.01) than PS0 (48.14%) and PK (43.14%). PS0 was significantly higher (P<0.01) than PK. Under conditions of these experiment it may be concluded that replacement of buffalo seminal plasma with bovine seminal plasma and diluted with lactose extender could improve of frozen semen quality of swamp buffaloes. Â Key words : Cattle seminal plasma, frozen semen, swamp buffalo

Effects of glycerol in tris extender on frozen semen quality of crossbred Etawah bucks Tambing, Surya Natal; Toelihere, Mozes R; Yusuf, Tuty L; Sutama, I ketut
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 2 (2000)
Publisher : Indonesian Animal Sciences Society

Bucks semen is easily damaged during freezing process, due to the formation of ice crystals. Consequently, semen quality decreases particularly the post-thaw sperm motility, viability, intack plasma membrane and intack acrosomal cap. The objectives of this research are to determine the optimum dose of glycerol in Tris extender in maintaining frozen semen quality of crossbred Etawah bucks. Four head of PE buck of 2 - 4 years old were used in this experiment. Doses of glycerol used were 5%, 6% and 7%. Semen was collected once a week using artificial vagina. Results of this experiment indicated that the mean percentage of pre-freezing motility, live sperm, sperm with intact plasma membrane and intact acrosomal cap in 5%, 6% and 7% glycerol were not significantly different (P>0.05). After freezing, the mean percentage of motility, live sperm, sperm with intact plasma membrane and intact acrosomal cap in 6% glycerol (52.60%, 65.03%, 45.63% and 47.54% respectively) were significantly higher (P<0.05) than in 5% (44.31%, 52.00%, 37.60% and 37.00% respectively) and in 7% glycerol (45.28%, 52.10%, 37.97% and 37.14% respectively). However, there were not significant differences in 5% and 7% glycerol for any parameter measured. It was concluded that supplementation of glycerol 6% in Tris extender effective to protect of sperm from various shock during the process of semen cryopreservation, so that it could maintain of frozen semen quality (sperm motility, viability, intack plasma membrane and intack acrosomal cap) that suitable used in AI program of crossbred Etawah bucks. Â Key words : Glycerol, PE bucks, semen quality

Effects of glycerol in tris extender on frozen semen quality of crossbred Etawah bucks Surya Natal Tambing; Mozes R Toelihere; Tuty L Yusuf; I ketut Sutama
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Bucks semen is easily damaged during freezing process, due to the formation of ice crystals. Consequently, semen quality decreases particularly the post-thaw sperm motility, viability, intack plasma membrane and intack acrosomal cap. The objectives of this research are to determine the optimum dose of glycerol in Tris extender in maintaining frozen semen quality of crossbred Etawah bucks. Four head of PE buck of 2 - 4 years old were used in this experiment. Doses of glycerol used were 5%, 6% and 7%. Semen was collected once a week using artificial vagina. Results of this experiment indicated that the mean percentage of pre-freezing motility, live sperm, sperm with intact plasma membrane and intact acrosomal cap in 5%, 6% and 7% glycerol were not significantly different (P>0.05). After freezing, the mean percentage of motility, live sperm, sperm with intact plasma membrane and intact acrosomal cap in 6% glycerol (52.60%, 65.03%, 45.63% and 47.54% respectively) were significantly higher (P<0.05) than in 5% (44.31%, 52.00%, 37.60% and 37.00% respectively) and in 7% glycerol (45.28%, 52.10%, 37.97% and 37.14% respectively). However, there were not significant differences in 5% and 7% glycerol for any parameter measured. It was concluded that supplementation of glycerol 6% in Tris extender effective to protect of sperm from various shock during the process of semen cryopreservation, so that it could maintain of frozen semen quality (sperm motility, viability, intack plasma membrane and intack acrosomal cap) that suitable used in AI program of crossbred Etawah bucks. Key words : Glycerol, PE bucks, semen quality

Effects of bovine seminal plasma on frozen semen quality of swamp buffaloes (Bubalus bubalis Muhammad Rizal Amin; Mozes R Toelihere; tuty L Yusuf; Polmer Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 3 (1999): SEPTEMBER 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Semen of two healthy swamp buffalo bulls were collected twice a week using artificial vagina. After initial evaluation, semen was divided into two parts, 1/3 for control (PK) and 2/3 for treatment (PS) and centrifugated at 3,000 rpm for 20 minutes. Seminal plasma of the second part was removed and changed with bovine seminal plasma for 0 minute (PS0) and 5 minutes (PS5) before semen was diluted with lactose extender containing 7% glycerol. Mean percentage of motility after thawing for PS5 (55.71%) was significantly higher (P<0.01) than PK (41.43%), but not significantly different with PS0 (52.43%). PS0 was significantly higher than PK. Mean percentage of live sperm and intact plasma membrane for PS5 (63.43% and 64.71%) were significantly higher (P<0.01) than PK (55.71% and 53.57%), but not significantly different with PS0 (61.14% and 59.28%). There was no significantly difference between PS0 and PK for mean percentage of live sperm and intact plasma membrane parameters. Mean percentage of intact acrosomal for PS5 (53.57%) was significantly higher (P<0.01) than PS0 (48.14%) and PK (43.14%). PS0 was significantly higher (P<0.01) than PK. Under conditions of these experiment it may be concluded that replacement of buffalo seminal plasma with bovine seminal plasma and diluted with lactose extender could improve of frozen semen quality of swamp buffaloes. Key words : Cattle seminal plasma, frozen semen, swamp buffalo

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