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All Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Kamsinah Kamsinah
Fakultas Biologi Unsoed
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Pertumbuhan Stek Krisan (Chrysanthemum morifolium (L.) Ramat) pada Berbagai Media Kultur In Vitro Adven Kristianti; Kamsinah Kamsinah; Murni Dwiati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.2.207

Abstract

Chrysanthemum (Chrysanthemum morifolium (L.) Ramat is one of ornamental plant commodities with high aesthetic value. As a consequence of increasing demand, the availability of seeds in adequate number is needed with method in vitro. This study aims to find out the effect of various in vitro culture media on the growth of chrysanthemum explants and to determine alternative media other than MS for in vitro growth of chrysanthemum explants. Experimental method arranged in a Randomized Complete Block Design (RCBD) with four treatments and four blocks is applied. The treatments are Murashige and Skoog  (MS), Vacint and Went  (V&W), Knudson  and   modified media, while node position serves as block. Time of shoot emergence, shoot length, number of leaves on each shoot, and number of roots are measured. Data are analyzed using Analysis of Variance (F test) with confidence intervals of 95% and 99%. Least Significant Difference (LSD) test is applied when significant difference among variables is observed. Culture media shows significant effect on chrysanthemum explant in vitro growth. Knudson media is found as an alternative media besides MS sufficiently suitable to increase chrysanthemum explants in vitro growth.
Respon Pemberian Hormon 2,4-D dan BAP terhadap Pertumbuhan Subkultur Kalus Kedelai (Glycine max (L.) Merrill) secara In Vitro Kiki Ayuningrum; Iman Budisantoso; Kamsinah Kamsinah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.296

Abstract

The purpose of this study was to evaluate the response of administration of a combination of 2,4-D and BAP on the growth of soybean callus subculture and determine the combination of 2,4-D and BAP most good for the growth of soybean callus subculture. The study design used completely randomized design (CRD) with a pattern factorials. The factor one e.i 2,4-D consists of 4 levels, namely: 0, 5, 10, and 15 ppm. A factor of 2 e.i BAP consists of 4 levels, namely: 0, 2, 4, dan 6 ppm. Every combination treatment repeated three times. Parameters measured include the percentage is growing callus, type of callus, dry weight and wet weight of soybean callus. The results showed that administration of the hormone 2,4-D and BAP can spur the growth of soybean callus subculture, the combination of BAP 2 ppm and 10 ppm of 2,4-D is the best combination for a percentage of callus and growing callus types, whereas the wet weight and the weight dried callus is not driven by a combination of the hormone 2,4-D and BAP
Co-Authors Adven Kristianti Iman Budisantoso Kiki Ayuningrum Murni Dwiati