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Journal : Jurnal Serambi Engineering

Ekplorasi dan Karakterisasi Biokimia Bakteri Resisten Timbal (Pb) dari Sungai Tallo Makassar Fahruddin Fahruddin; Nur Haedar; Slamet Santosa; Sri Wahyuni
Jurnal Serambi Engineering Vol 5, No 3 (2020)
Publisher : Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32672/jse.v5i3.2133

Abstract

Industrial progress and waste of urban domestic activities have an impact on the Pb heavy metal pollution on the environment, this will have an impact on human health. Pb metal can be overcome with biological methods by utilizing bacteria in reducing Pb metal. The study aimed to obtain the species  of resistant bacterial isolates of Pb metal and determine its characteristics. Sediment and water samples were obtained from the Tallo River. Isolation and selection of Pb metal resistant bacteria were carried out on nutrient agar media is 10 ppm added of PbAgNO3, afterwards the colonies that grew and differed were characterized by morphological morphology and several biochemical tests. The characterization results obtained 8 isolates of Pb metal resistant bacteria consisted of 8 isolates from sediment samples and 3 isolates from water samples. The characteristics of each bacterial isolate on cell morphology are the same and biochemical tests show different results.
Isolasi dan Karakteristik Bakteri Pendegradasi Selulosa dari Limbah Pusat Industri Mebel Antang Makassar Fahruddin Fahruddin
Jurnal Serambi Engineering Vol 5, No 2 (2020)
Publisher : Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32672/jse.v5i2.1922

Abstract

Antang Furniture Center in Makassar is one of the wood industries and is still traditionally. The wood processing produces waste containing cellulose that is difficult to degrade. This study aims to isolate and observe the characteristics of cellulose degrading bacteria. This research was conducted at the Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar from March 2019 to November 2019. The method used was an experimental laboratory consisting of Carboxyl Methyl Cellulose (CMC) media for bacterial selection and characterization including microscopic morphology with Gram staining and morphology macroscopic with colony structure. The results showed that cellulose degrading bacteria were characterized by the formation of clear zones formed on CMC media in petri dishes. Based on macroscopic and microscopic morphological characteristics, eleven types of cellulose degrading bacteria isolates were obtained. The isolates obtained can be a source of bacterial isolates for further application in wood waste or other wastes that contain of cellulose.