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All Journal Jurnal Ilmu Alam dan Lingkungan
Andi Ilham Latunra
Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Hasanuddin, Makassar 90245 Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Veterinary

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Efektivitas Ekstrak Daun Eceng Gondok Eichornia crassipes Sebagai Bahan Antikanker pada Sel Tumor MCF-7 dengan Metode In Vitro dan In Silico Eva Johannes; Andi Ilham Latunra; Mustika Tuwo; Sry Sukmawaty
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 1 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i1.13095

Abstract

The use of water hyacinth Eichhornia crassipes bioactive compounds has been developed, including as an anti-cancer base material. This study aims to examine the potential of water hyacinth leaf extract on MCF-7 tumor cells with the MTT method and the affinity of the water hyacinth leaf compound using molecular docking methods. The results showed the effect of water hyacinth leaf extract on MCF-7 cancer cell death due to the active compounds stigmasterol and phytol, while the docking results showed the best binding affinity value on stigmasterol and its receptor 1QR1 which was -9.4.
Analisis Kandungan Kafein Kopi (Coffea arabica) Pada Tingkat Kematangan Berbeda Menggunakan Spektrofotometer UV-VIS Andi Ilham Latunra; Eva Johannes; Besse Mulihardianti; Ophirtus Sumule
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 1 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i1.13096

Abstract

A study recently conducted with the title Analysis of caffeine content in arabica coffee (Coffea arabica) based on its maturity level by using Uv-Vis Spectrophotometer. This study aimed to determine the levels of caffeine in Arabica coffee based on its level of maturity, either young, medium and old. Moreover, this study aimed to measure the highest and lowest caffeine levels of Arabica coffe from. Retrieval of young coffee fruit is characterized by green skin fruit, half-old / medium coffee, yellow to orange skin fruit and dark coffee with dark-red skin fruit. The Fruit of Arabica coffee is processed starting from drying, roasting and grinding into coffee grounds. Determination of caffeine content using 2 methods, namely a qualitative test with the parry method and a quantitative test using a UV- Vis spectrophotometer. The results obtained in the qualitative test with the Parry method showed that all three coffee powder samples contained caffeine, this was indicated by a change in color into green. Quantitative test results using UV-Vis spectrophotometer obtained the results of each gram of coffee containing caffeine namely young arabica coffee 11.15 mg or 1.151%, half old / medium arabica 12.85 mg or 1.285 % and old arabica coffee 12.01 mg or 1.201 %. The highest level of caffeine is found in the level of maturity of half-old / moderate coffee and the lowest level of caffeine is found in Arabica coffee with a young age of maturity.
Co-Authors Besse Mulihardianti Eva Johannes Mustika Tuwo Ophirtus Sumule, Ophirtus Sry Sukmawaty