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All Journal The Journal of Experimental Life Sciences (JELS) Food Science and Technology Journal (Foodscitech) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal Of Aquaculture Development and Environment JURNAL AGROSAINS : Karya Kreatif dan Inovatif
Sugiono Sugiono
Departement Fisheries Agrobusiness, Faculty Of Agriculture, Universitas Islam Madura, Pamekasan 69351, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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ISOLASI DAN KARAKTERISASI FUKOIDAN DARI ALGA COKLAT Sargassum sp Sugiono Sugiono
JURNAL AGROSAINS : Karya Kreatif dan Inovatif Vol 2 No 1 (2015): Juni 2015
Publisher : Universitas Islam Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31102/agrosains.2015.2.1.97-107

Abstract

Fukoidan dari alga coklat Sargassum sp. dengan ekstraksi satu tahap degradasi awal gelombang ultrasonik.Optimasi ekstraksi dengan rancangan komposit pusat dari RSM.Kondisi degradasi awal gelombang ultrasonic pada amplitude 80% selama 15 menit.Alga coklat dilarutkan dalam larutan 0,03 M HCl ratio 1:20 (b/v) pada suhu 70-90oC waktu 3-5 jam, dievaluasi selama proses untuk mendapatkan kondisi ekstraksi yang maksimal. Tujuan penelitian ini adalah untuk mendapatkan kondisi ekstraksi yang optimal respon rendemen fukoidan Sargassum sp.Hasil penelitian didapatkan semua faktor ekstraksi berpengaruh nyata tehadap rendemen fukoidan (p<0,05). Kondisi ekstraksi optimal terjadi pada suhu 81,0oC dan waktu 4,04 jam. Pada titik optimal ekstraksi didapatkan rendemen fukoidan 7,15±5,58%.Karakterisasi fukoidan berdasarkan spektrum infra merah didapatkan fukoidan Sargassum sp. tersusun dari gugus sulfat.
EKSTRUDER ULIR GANDA: PENGARUH KONDISI PROSES TERHADAP VISKOSITAS INTRINSIC ALGINAT DARI Sargassum cristaefolium Sugiono Sugio; Teti Estiasih; Masruri Masruri; Simon Bambang W.
JURNAL AGROSAINS : Karya Kreatif dan Inovatif Vol 3 No 1 (2016): Juni 2016
Publisher : Universitas Islam Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31102/agrosains.2016.3.1.278-285

Abstract

Tujuan penelitian ini untuk menentukan kondisi proses ekstrusi yang memberikan nilai maksimum respon viskositas intrinsic. Rancangan factorial 2k digunakan untuk mengetahui pengaruh suhu, kec. ulir dan pH terhadap viskositas intrinsik dan menentukan ketepatan model polynomial orde pertama. Perlakuan suhu, kec. ulir dan pH ekstraksi alginat proses ekstrusi memberikan pengaruh signifikan terhadap viscositas intrinsik. Nilai maksimum viskositas intrinsik alginat 416.16 ml/g terjadi pada kondisi proses ekstrusi suhu 60oC, kec. ulir 75 rpm dan pH 10. Model polinomial orde pertama berbentuk kuadratik,perencanaan titik pusat penelitian suhu 60oC, kec. Ulir 75 rpm dan pH 10 adalah tepat. Keywords:Alginat, Ekstruder ulir ganda, viscositas instrinsik, Sargassum cristaefolium
Extraction of Sequential Fucoidan and Alginate Biorefinery for Pre-extraction Acid Treatment of Intrinsic Alginate Viscosity of Brown Algae Doni Ferdiansyah; Sugiono Sugiono
Journal of Aquaculture Development and Environment Vol 3, No 1 (2020): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v3i1.2355

Abstract

Efek Penggunaan Pelarut Berulang pada Proses Pre-Ekstraksi Terhadap Mutu Alginat dari Sargassum cristaefolium Sugiono Sugiono; Alfan Nur Abadi; Sulfiatus Zannuba; Alvin Taufiky; Matheus Nugroho
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): Desember 2022
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v17i2.804

Abstract

Pre-ekstraksi alginat dari alga coklat dengan pelarut asam membutuhkan reaktan dan air dalam jumlah banyak, serta menghasilkan mengeluarkan banyak limbah. Penelitian ini bertujuan untuk mengoptimalkan penggunaan pelarut asam dengan melakukan penggunaan pelarut secara berulang dan mengetahui pengaruhnya terhadap mutu alginat, reduksi kebutuhan air dan reaktan, serta reduksi limbah pelarut. Penggunaan kembali pelarut asam pada proses pre-ekstraksi dilakukan dengan variasi penggunaan pelarut ke 0, 1, 2, 3, dan 4. Rancangan acak lengkap dengan tiga kali ulangan digunakan untuk menentukan pengaruh penggunaan pelarut berulang terhadap mutu alginat. Parameter yang diamati meliputi rendemen, viskositas, berat molekul alginat, kebutuhan air dan HCl, serta limbah pelarut asam. Hasil penelitian menunjukkan bahwa penggunaan pelarut berulang dapat menghasilkan alginat dengan rerata rendemen 26,7%. Penggunaan pelarut berulang ke 0-2 dapat menghasilkan alginat kualitas sedang dengan viskositas 270,9-360,0 mPa.s dan berat molekul alginat 122,04-175,44 kDa. Pre-ekstraksi makrolaga dengan pelarut berulang ke 0-2 dapat menurunkan kebutuhan air 22,2%, HCl 66,7%, dan limbah pelarut menurun 66,7%. Pre-ekstraksi makroalga dengan pelarut berulang mempunyai potensi efisiensi dan perlu dilakukan penelitian lebih lanjut pada skala yang lebih besar. AbstractPre-extraction of alginate from brown algae with acidic solvents requires a lot of reactants and water that produce a lot of solvent wastes. This study aimed to optimize the use of acidic solvents by using solvents repeatedly and to determine the effect on alginate quality, water consumption and reactant, and acid solvent waste. Repeated use of solvents on pre-extraction stage was carried out with variations of 0, 1, 2, 3, and 4 times. A Completely Randomized Design (CRD) with three replications was used to determine the effect of repeated use of solvents on the alginate quality. Parameters observed were yield, viscosity, molecular weight of alginate, water consumption, HCl requirement, and the amount of acid solvents waste. The result showed that the repeated use of solvents in the pre-extraction stage produced alginate with an average yield of 26.7%. Repeated use of solvents of 0 to 2 times produced medium quality of alginate with a viscosity of 270.9-360.0 mPa.s and alginate molecular weight of 122,04-175,44 kDa. Pre-extraction of macroalgae by repeated use of solvents can reduce water consumption 22,2%, HCl 66,7%, and waste solvents 66,7%. Pre-extraction of macroalgae by repeated use of acid solvents was potential for efficiency, and further research is needed for a larger scale extraction process.
ANALISIS PENGELOLAAN PELABUHAN PERIKANAN PANTAI PASONGSONGAN KABUPATEN SUMENEP UNTUK PENINGKATAN PRODUKSI PERIKANAN TANGKAP Ruhah Ruhah; Sugiono Sugiono; Doni Ferdiansyah
JURNAL AGROSAINS : Karya Kreatif dan Inovatif Vol 7 No 2 (2022): JURNAL AGROSAINS : Karya Kreatif dan Inovatif
Publisher : Universitas Islam Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31102/agrosains.2022.7.2.95-106

Abstract

Iincreased capture fisheries production at UPT. P3 Pasongsongan is supported by an increase in the number of visiting ships. This in crease in productionmust bebalanced whit proper management. The porpose of this study was to determine the condition of the facilities at UPT. P3 Pasongsongan, knows the management of activities and facilities at UPT. P3 Pasongsongan, and analyzes the right strategy to be applied in the management of P3 Pasongsongan, so that it can provide useful outputs for all aspects related to P3 Pasongsongan. The method used in this research is descriptive method with SWOT analysis to identify various factors in order to formulate a good and appropriate management strategy.based on the results of the analysis that the existing facilities at UPT. P3 pairs are in good condition and still suitable for use, likewise the activities starting from fish marketing, fish processing, and fish marketing are going well, the only constraints are that TPI is not good and ther is no coold storage that can hinder activities in UPT. P3 Psongsongan. While the SWOT anlysis obtained is the result of imlementing the S-O strategy (Streght-Opportunity) which in its applications uses strength to take advantage of opportunities.
Microwave-Assisted Extraction in A Sequential Biorefinery of Alginate and Fucoidan From Brown Alga Sargassum Cristaefolium Sugiono Sugiono; Mohammad Taufiq Hidayat; Faruk Alrosyidi; Alfan Nur Abadi; Khairul Anam; Sulfiatus Zannuba; Alvin Taufiky; Emrin Hoiriyah; Matheus Nugroho
Food Science and Technology Journal (Foodscitech) Vol. 5 No. 2 (2022)
Publisher : Universitas Dr Soetomo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.492 KB) | DOI: 10.25139/fst.vi.4976

Abstract

The brown alga has bioactive alginate and fucoidan, a potential for raw materials the biorefinery industry with core processing of fucoidan and alginate extraction. The research on sequential biorefinery of fucoidan and alginate was conducted in conventional and hydrothermal methods using a smart pressure-cooker, but the yield of fucoidan is low. The improvement was carried out by extracting fucoidan and alginate using microwave-assisted extraction (MAE). This study aimed to obtain the MAE processing conditions that produced the maximum yield of fucoidan and alginate. The factorial design of 2k was used to determine the effect of MAE processing conditions, i.e. temperature, time, and alga/water ratio on fucoidan and alginate yields. The parameters observed were fucoidan and alginate yield. Data analysis and prediction of first-order model and model accuracy was a regression modelling using a design expert program. The result showed that the elevate of temperature MAE processing to 80 oC,for 20 minutes, and alga/water ratio of 2:20 (w/v) increased fucoidan (4.5%) and alginate (40.6%) yield, but it decreased the yield at 100 oC, for 30 minute, and alga/water ratio of 3:30 (w/v). The equation of the first-order model was quadratic, by wich the model test in curvature was found significant at α=0.05. The MAE sequential biorefinery extraction improved fucoidan and alginate yield and had a potential efficiency for commercialization. The maximum response of fucoidan and alginate yield was obtained from a MAE sequential biorefinery at 80 oC,for 20 minutes,and alga/water ratio 2:20 (w/v); further research is needed to optimizing the process.
Shelf Life and Quality Stability Determination of Drying Shredded Dumbo Catfish (Clarias Gariepinus) Nugroho, Matheus; Susanti, Eli; Sugiono, Sugiono
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.06

Abstract

The purpose of this study was to determine the stability of the quality and shelf life of dried shredded African catfish (Clarias gariepinus). The method used in this study was quantitative research using an experimental method using a completely randomized design with three different drying treatments. The stability of the drying quality of African catfish shredded (Clarias Gariepinus) was the color of African catfish shredded with varying methods of drying, giving panelist scores ranging from 2.36 to 4.4; organoleptic value of African catfish floss ranged from 2.88 to 4.16; panelists' assessment of the texture of African catfish shredded ranged from 2.12 - 4.56; the taste of African catfish floss ranges from 2.08 to 4.44. Shredded African catfish with different drying durations that were most in demand by panelists was sample A3 shredded African catfish steamed and dried for 4 minutes in a spinner machine with a color score of 4.4, aroma 4.16, texture 4.56, and taste 4.44.  The results of measurements using distribution turnover explained that the shelf life of shredded catfish could last up to 7 months and was reduced to 6 months to anticipate product damage earlier. Keywords: quality, shelf life, shredded dumbo catfish.
Integrated and Sequential Enzyme-Assisted Extraction of Fucoidan and Alginate from Sargassum echinocarpum Sugiono, Sugiono; Hidayat, Mohammad Taufiq; Wahyuni, Endang Tri; Syafii, Syafii; Fikriyatullatifah, Anisa; Mumtazah, Lutfia Anis
The Journal of Experimental Life Science Vol. 15 No. 2 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.02.05

Abstract

The sequential extraction biorefinery process can use brown algae as its feedstock, offering a sustainable approach to maximizing the utilization of marine biomass for high-value bioactive compounds. This method enables the recovery of multiple bioactive components from a single raw material, increasing process efficiency and reducing waste. Fucoidan and alginate, two valuable polysaccharides in brown algae, have attracted significant attention due to their diverse applications in the pharmaceutical, nutraceutical, and food industries. This research aims to develop an enzyme-assisted extraction (EAE) process that maximizes the fucoidan yield of Sargassum echinocharpum in the integrated alginate and fucoidan extraction biorefinery process. The effect of the parameter process, including temperature, time, and solvent-to-algae ratio, was evaluated to maximize fucoidan and alginate yield and determine the correct equation model in the first order of the experiment. The study showed that temperature, time, and the ratio of solvents all had a real effect on how quickly the fucoidan and alginate yields increased and then decreased after reaching their highest point. The temperature of 50°C, time of 24 hours, and the solvent-to-algae ratio of 30:1 (v/w) resulted in the highest fucoidan yield of 4.25% and alginate yield of 35.3%,  along with an intrinsic viscosity of 78.91 mL.g-1, molecular weight of 73.58 kDa, and an M/G ratio of 0.35.  Statistical analysis revealed a quadratic relationship, suggesting the presence of an optimal extraction condition. These findings highlight the potential of enzyme-assisted biorefinery techniques for improving fucoidan and alginate extraction. This extraction process demonstrates significant potential for industrial-scale application in the food, pharmaceutical, and nutraceutical industries due to its efficiency and sustainability.  
Co-Authors Alfan Nur Abadi Alfan Nur Abadi Alvin Taufiky Alvin Taufiky Doni Ferdiansyah Eli Susanti Emrin Hoiriyah Endang Tri Wahyuni Faruk Alrosyidi Fikriyatullatifah, Anisa Khairul Anam Masruri Masruri Matheus Nugroho Mohammad Taufiq Hidayat Mohammad Taufiq Hidayat Mumtazah, Lutfia Anis Ruhah Ruhah Simon Bambang W. Sulfiatus Zannuba Sulfiatus Zannuba Syafii . Teti Estiasih