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All Journal HAYATI Journal of Biosciences Tropical Medicine Journal Molluca Journal of Chemistry Education (MJoCE) Indonesian Journal of Pharmacology and Therapy
Rachel Turalely
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Agriculture, Biological Sciences & Forestry Chemistry Earth & Planetary Sciences Education Immunology & microbiology Medicine & Pharmacology

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UJI AKTIVITAS ANTIPLASMODIUM FRAKSI 12 (FG12) EKSTRAK METANOL DAUN KAPUR (HARMSIOPANAX ACULEATUS HARMS) Turalely, Rachel
Molluca Journal of Chemistry Education (MJoCE) Vol 8 No 2 (2018): Molluca Journal of Chemistry Education (MJoCE)
Publisher : Prodi Pendidikan Kimia FKIP Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/MJoCEvol8iss2pp71-75

Abstract

Daun kapur (Harmsipanax aculeatus Harms) merupakan tanaman tradisoanal dari Maluku yang digunakan secara turun temurun untuk mengobati malaria. Secara ilmiah, ekstrak daun kapur telah diujikan aktivitas antiplasmodium secara in vivo dan in vitro. Ekstrak metanol daun kapur secara in vivo maupun in vitro telah terbukti memiliki aktivitas antiplasmodium sehingga berpotensi dikembangkan untuk mencari senyawa aktif antimalaria.  Ekstrak metanol daun kapur telah dipisahkan lebih lanjut dengan vacuum liquid chromatography dan dimonitoring dengan thin layer chromatography sehingga diperoleh 12 fraksi. Fraksi 12 ekstrak metanol  larut dalam metanol. Namun aktivitas sebagai antiplasmodium belum pernah dilaporkan. Penelitian ini menjelaskan tentang aktivitas antiplasmodium FG12 ekstrak metanol. Uji aktivitas antiplasmodium dilakukan secara in vitro dan persentase penghambatan parasitemia yang diperoleh kemudian dianalisis dengan analisis regresi probit menggunakan SPSS sehingga dapat diperoleh nilai IC50.  Hasil penelitian menunjukkan bahwa nilai IC50  FG12 sebesar 3,88 µg/mL. Nilai IC50 ini menunjukkan bahwa FG12 ekstrak metanol daun kapur memiliki aktivitas antiplasmodium paling baik dan berpotensi dikembangkan untuk mencari dan menemukan senyawa aktif antimalaria dari daun kapur.
In vivo Antiplasmodial of the Most Active Fraction and Its Compound of Kapur Leaves (Harmsiopanax aculeatus Harms) Extract Against Plasmodium berghei Rachel Turalely; Ratna Asmah Susidarti; Mahardika Agus Wijayanti
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (992.339 KB) | DOI: 10.22146/tmj.4575

Abstract

Introduction : The rising of Plasmodium resistance towards chloroquine and other antimalarial drugs have encouraged to discover and develop new drugs mainly derived from natural products. Harmsiopanax aculeatus (kapur plant) has traditionally used by people of in Maluku Province to treat malaria.Objectives: The aims of this study were to identify antiplasmodial activity and its chemical constituents of the most active fraction of kapur leaves.Methods: The dried powder of Kapur leaves (1.3 kg) were extracted successively by maceration with n-hexane, ethyl acetate and methanol. After removal the solvents the hexane 15.6 g (1.2%), ethyl acetate 53.3 g (4.1%) and methanol 61.1 g (4.7%) extracts were obtained. Those extracts were assayed for their in vivo antiplasmodial activities by using 4-days suppressive test in Swiss mice infected with Plasmodium berghei, HPIA and identified the compound by GC-MS.Results: The ED50 of hexane, ethyl acetate and methanol extracts were 467.58, 2074.02 and 16.16 mg/kgBW, respectively. Fractionation of the methanol extract gave 18 combined fractions (FG1 – FG18). FG8 was the most active fraction with the IC50 HPIA of 18.22 μg/ml. Phytochemical test of this fraction using spray reagent showed the existence of essential oils, triterpenoids, and phenolic compounds. Separation of FG8using pressed chromatography gave 19 combined fractions (FG8.1-FG8.19). The fraction containing intense blue fluorescent spot (FG8.5) was further separated by PLC fourthly eluted with chloroform. Seven major components with the percentage of compotition more than 3.11% were identified as eugenol (tr = 12.692; 18.22%), isoprophyl myristate (tr = 16.333; 3.99%); bis(2-methylpropyl) phtalat (tr = 16.939; 7.15%); methyl palmitic (tr = 17.442; 3.11%); palmitic acid (tr = 17.883; 25.72%); butyl 2-methylpropyl phtalat (tr = 17.957; 9.37%) and bis(2-ethylhexyl) phtalat (tr = 23.258; 23%).Conclusion: Methanol extract of H. aculeatus was the most potential in vivo antiplasmodial activity. Combined fraction 8 which contain 7 compounds was the most active fraction.Keywords: Harmsiopanax aculeatus Harms, in vivo antiplasmodial, HPIA, PLC, GC-MS
Antiplasmodial Activity, Cytotoxicity, and Active Compounds Analysis of Active Fraction of Harmsiopanax aculeatus Leaves from Maluku, Indonesia Turalely, Rachel; Mustofa; Hertiani, Triana; Wijayanti, Mahardika Agus
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.879-895

Abstract

The leaves of the H. aculeatus plant are known as a traditional antimalarial medicinal plant in Maluku. Several studies have reported on the leaf activity of this plant, but studies on in vitro and in vivo antiplasmodial activity and the compounds in the active fraction (F7) of H.aculeatus leaf have not been reported. This study aims to determine the antiplasmodial activity of the F7 of H. aculeatus leaves and to analyze the compounds contained therein. In vitro, antiplasmodial activity was tested on Plasmodium falciparum strain FCR3 using a microscopic method. A cytotoxicity test was performed on Vero cells using an MTT assay. In vivo, the antiplasmodial activity of F7 was carried out using the 4-day suppressive test method by treating Swiss mice infected with P. berghei. Analysis of the compounds in an F7 was done using spray reagent and UV Vis DAD and HPLC DAD-MS with a UV detector. The results showed that the F7 was very active in vitro (IC50 of 0.7 µg.ml-1) and in vivo (ED50 of 2.49 mg.kg BW-1.d-1) also selectively (SI of 8159.94) inhibited the growth of Plasmodium. The F7 contains a group of essential oils, triterpenoids, phenolic compounds, and flavonoids after being analyzed using spray reagents. Based on the analysis results, five compounds were identified: fomoxanthone, cyclopentene, microspherone, indole 3-carbaldehyde, and naamine. In addition, seven compounds were not identified.
Antiplasmodial activity and cytotoxicity of kapur (Harmsiopanax aculeatus) leaf extracts traditionally used for the treatment of malaria in Maluku Turalely, Rachel; Mustofa; Mahardika Agus Wijayanti; Triana Hertiani
Indonesian Journal of Pharmacology and Therapy Vol 1 No 1 (2020)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.512

Abstract

Harmsiopanax aculeatus leaf, locally name kapur, has been use traditionally to treat malaria in Maluku, Indonesia. However, the scientific evidences that support its use are still limited. This study aimed to investigate antiplasmodial activity of H. aculeatus leaf extract and its cytotoxicity on cancer cells line. Three extracts i.e. methanolic, n-hexanic and ethyl acetate extracts were evaluated for their in vitro activity against Plasmodium falciparum FCR3 strain using microscopic method. Cytotoxicity of the extracts on T47D, HeLa and Vero cells lines were determined using MTT assay method. The inhibitory concentration 50% (IC50) against P. falciparum or the cells lines growth was determined using probit analysis. Furthermore, their selectivity index (SI) were determined. The results showed that the methanolic extract was the most active extract with an IC50value of 13.82 μg/mL and the most selective with a SI value of 172.84. The three extracts tested exhibited weak or no cytotoxicity against the cells lines used with IC50 values ranged 101-2388.69 μg/mL. Further study will be conducted to isolate active antiplasmodial compounds from the methanol extract.
Co-Authors Mahardika Agus Wijayanti Mahardika Agus Wijayanti Mustofa Mustofa Ratna Asmah Susidarti Triana Hertiani