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All Journal HAYATI Journal of Biosciences Tropical Medicine Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) WMJ (Warmadewa Medical Journal) Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) INDONESIAN JOURNAL OF PHARMACY JFM (Jurnal Farmasi Malahayati) PUBLIKASI PENELITIAN TERAPAN DAN KEBIJAKAN Molluca Journal of Chemistry Education (MJoCE)
Mahardika Agus Wijayanti
Departement Of Parasitology, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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PERBEDAAN GEJALA KLINIS DAN EFEK SAMPING PENGOBATAN PADA MALARIA FALCIPARUM DAN VIVAX Santoso, Santoso; Supargiyono, Supargiyono; Wijayanti, Mahardika Agus
Publikasi Penelitian Terapan dan Kebijakan Vol 6 No 2 (2012): Jurnal Pembangunan Manusia
Publisher : Badan Penelitian dan Pengembangan Daerah Provinsi Sumatera Selatan

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Abstract

Malaria masih menjadi masalah kesehatan masyarakat di Indonesia. Daerah endemis malaria terus meningkat yang diiringi dengan peningkatan kasus resistensi terhadap obat anti malaria. Kabupaten Ogan Komering Ulu (OKU) merupakan salah satu daerah endemis malaria di wilayah Provinsi Sumatera selatan dengan Annual Malaria Insidence (AMI) tahun 2008 sebesar21,79 per mil. Jenis malaria yang sering berkembang menjadi malaria berat adalah malaria falsiparum dengan gejala umum yang sering dijumpai diantaranya demam, menggigil dan berkeringat. Gejala klinis ini sering tidak dijumpai pada penderita malaria vivak sehingga penderita malaria vivaks seringkali tidak ditemukan. Sesuai dengan kebijakan Depkes maka sejak tahun2004 pengobatan malaria falsiparum menggunakan obat baru kombinasi artemisinin. Sedangkan untuk pengobatan malaria vivaks baru dimulai tahun 2008. Kegiatan pengobatan malaria dengan menggunakan obat kombinasi artemisinin sering menimbulkan efek samping sehingga menimbulkan pengobatan malaria seringkali tidak sesuai dosis. Berdasarkan hal tersebut maka dilakukan penelitian yang bertujuan untuk mengetahui perbedaan gejala klinis dan efek samping pemberian obat anti malaria pada penderita malaria falciparum dan malaria vivax. Jumlah penderita malaria yang ditemukan sebanyak 35 orang, yaitu 23 penderita malaria falciparum dan 12 orang penderita malaria vivax. Gejala klinis yang ditemukan pada penderita malaria berupa menggigil, sakit kepala, pusing, anoreksia dan nyeri otot. Gejala klinis awal sebelum terapi lebih banyak ditemukan pada penderita malaria falciparum (91%) dibandingkan pada penderita malaria vivax (50%). Hasil uji statistik menunjukkan ada perbedaan yang bermakna antara penderita malaria falciparum dengan penderita malaria vivax terhadap munculnya gejala klinis awal. Efek samping artesdiakuin yang ditemukan berupa gatal, pusing, mual, muntah dan nyeri lambung.
HUBUNGAN ANTARA FAKTOR EKOLOGI TERHADAP KEPADATAN PUPA AEDES AEGYPTI SEBAGAI VEKTOR PENYAKIT DEMAM BERDARAH DENGUE DI KOTA BANDAR LAMPUNG Selvi Marcellia; Sitti Rahmah Umniyati; Mahardika Agus Wijayanti
Jurnal Farmasi Malahayati Vol 2, No 1 (2019): Jurnal Farmasi Malahayati
Publisher : Jurnal Farmasi Malahayati (JFM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.105 KB) | DOI: 10.33024/jfm.v2i1.1546

Abstract

Penyakit demam berdarah dengue (DBD) merupakan penyakit yang ditularkan melalui gigitan nyamuk Aedes aegypti dan Aedes albopictus. Jumlah kasus DBD tertinggi di salah satu provinsi yang ada di Indonesia yaitu di Provinsi Lampung. Kota Bandar Lampung yang merupakan bagian dari Provinsi Lampung memiliki jumlah kasus DBD tertinggi karena jumlah penduduk yang cukup padat. Nyamuk Ae. aegypti berkembang biak dengan baik pada daerah yang memiliki ketinggian kurang dari 1000 m dpl dengan suhu antara 25o-30oC dan kelembaban optimal yang dibutuhkan antara 65%–80%. Tujuan dari penelitian ini yaitu mengetahui faktor ekologi yang memiliki kontribusi paling tinggi terhadap kepadatan pupa Ae. aegypti sebagai vektor penyakit Demam Berdarah Dengue (DBD) di Kota Bandar Lampung. Penelitian ini merupakan jenis penelitian analitik observasional dengan rancangan studi cross sectional dengan pengamatan yang dilakukan one point in time. Populasi dalam penelitian ini adalah semua larva dan pupa Ae. aegypti yang dikoleksi dari Kelurahan Sukaraja, Kelurahan Korpri Raya, dan Kelurahan Gunung sulah yang termasuk wilayah endemis di Bandar Lampung. Kelurahan Korpri Raya di Kota Bandar Lampung memiliki faktor ekologi seperti temperatur udara, kelembaban udara, dan ketinggian tempat paling berpengaruh terhadap kepadatan populasi pupa Ae. aegypti karena nilai adjusted R2 pada Kelurahan Korpri Raya paling tinggi yaitu 20,9%. Berdasarkan nilai Standarized Coeficient Beta masing-masing wilayah sebesar 0,388; 0,255; 0,652 menunjukkan bahwa pada Kelurahan Gunung Sulah dan Kelurahan Korpri Raya variabel yang memiliki hubungan paling berpengaruh terhadap kepadatan populasi pupa Ae. aegypti adalah temperatur udara.
Taeniasis in Karangasem Regency, Bali Ni Wayan Winianti; Elsa Herdiana M; Mahardika Agus Wijayanti; Putu Sutisna; I Nengah Kapti; I Wayan Sudiarta
WMJ (Warmadewa Medical Journal) Vol 3 No 1 (2018): May 2018
Publisher : Warmadewa University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wmj.3.1.651.1-5

Abstract

Abstract Taeniasis is an infectious disease caused by the tapeworm Taenia saginata (beef tapeworm), Taenia solium (pork tapeworm), and Taenia asiatica (Asian tapeworm). Purpose of the study was to determine the relationship between meat consumption, individual characteristics, and environmental factors with taeniasis. Case control study was used to identify taeniasis among two groups: people living at Datah village (personal livestock) and people living at Karangasem village (obtained their meat from traditional market or slaughterhouse). Each group consisted of 98 people. Two subjects from the case group were positive for taenia egg both by direct smear and Willy’s Malory floatation methods. Proportion of taeniasisinthe case group was 2.04%. The type of meat consumed, methods of meat processing, frequency of consumption, ways of obtaining meat, individual characteristics, and environmental factors which may contribute to Taenia infection could not be evaluated due to limited number of positive cases. Abstrak Taeniasis merupakan penyakit infeksi yang disebabkan karena adanya cacing pita Taenia saginata (beef tapeworm), Taenia solium (pork tapeworm), and Taenia asiatica (Asian tapeworm)di dalam tubuh manusia. Tujuan penelitian ini untuk mengetahui hubungan antara konsumsi daging, karakteristik individu dan faktor lingkungan dengan kejadian taeniasis. Jenis rancangan penelitian yang digunakan yaitu case control antar 2 kelompok (penduduk di Desa Datah dan Kelurahan Karangasem, Kabupaten Karangasem, Bali). Kelompok kasus yaitu penduduk Desa Datah yang memenuhi kriteria kasus (mengkonsumsi daging dari hewan ternak pribadi), sedangkan kelompok kontrol yaitu penduduk di Kelurahan Karangasem yang memenuhi kriteria untuk kontrol (mengkonsumsi daging dari membeli di pasar atau tempat pemotongan hewan). Tiap kelompok terdiri dari 98 orang. Metode pemeriksaan feses dengan metode langsung dan pengapungan. Terdapat 2 orang yang positif taeniasis dari kelompok kasus, dengan ditemukan telur Taenia sp. pada pemeriksaan langsung dan pengapungan. Hubungan jenis daging, cara pengolahan, frekuensi mengkonsumsi dan cara memperoleh daging, karakteristik individu dan faktor lingkungan dengan kejadian taeniasis tidak dapat dianalisis secara statistic.
The Effect of Pandanus conoideus Lamk Extract to the Serum Level of TNF-α, IL-10 and Parasitemia of Plasmodium berghei Infected in Mice Zeth Robeth Felle; Mahardika Agus Wijayanti; Supargiyono Supargiyono
Tropical Medicine Journal Vol 3, No 1 (2013): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1210.354 KB) | DOI: 10.22146/tmj.5839

Abstract

ABSTRACT Introduction: Study on the effects of red fruit (Pandanusconoideus Lamk) has been conducted with various result.Objectives: In this study, the effect of red fruit extract on the level of cytokines TNF-α, IL-10 and the parasitemia of Plasmodium berghei infected Swiss mice were evaluated.Methods: Quasi-experimental design with pre and post test only control group was applied. Sixty male Swiss mice of 8 weeks old and weighs 20-30 g, was simply randomized into four treatment groups. Group I (K1) was stimulated with the extract for 2 weeks before and 2 weeks after infection with P.berghei. K2 was stimulated with the extract for 2 weeks before infection, K3 was stimulated with the extract for 2 weeks after infection and K4, negative control, was given 0.6% tween 40. The dose of the extract was 7.8 mg/30g mice BW, intra gastric once a day. Serum level of TNF-α and IL-10 was measured by ELISA Sandwich methods and the number of parasitemia were examined microscopically. The difference level of TNF-α, IL-10 and parasitemia of each treatment group were analyzed by t-test, one way anova, honestly significant different (HSD) and multivariate analysis (manova).Results: There were significan cedifferences of parasitemia in K3 group compared to others. Parasitemia on day-3 was 18.464% and reduced to 1.054% on day-9. Parasitemia of K2 group was 13.204% on day-3 and 32.455% on day-9. Parasitemia of negative control group was 27.304% on day-3 and 78.506% on day-9. The TNF-α level of K3 group decreased along with the infection, it was 26.985 pg/Ml on day-0 and 22.244 pg/mL on day-6. The IL-10 level increased at all groups and the highest level was on K3 group.Conclusion: Effect of red fruit extract on P.berghei infected mice was reduced both parasitemia and TNF-α level but increased of IL-10 level.Keywords : Pandanus conoideus Lamk, Malaria, Parasitemia, TNF-α, IL-1
Prevalence and risk factors of intestinal protozoan infection among child students with disabilities in Bantul District, Yogyakarta Special Region, Indonesia Fahriana Azmi; Elsa Herdiana Murhandarwati; Mahardika Agus Wijayanti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 53, No 2 (2021)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.087 KB) | DOI: 10.19106/JMedSci005302202109

Abstract

Children with disabilities are excluded from many aspects of life. Unfortunately, they have an increased risk of infection from many kinds of pathogens including intestinal protozoan. This study aimed to determine the prevalence of intestinal protozoan infections and to evaluate the associated factors among children with disabilities in Bantul District, Yogyakarta Special Region, Indonesia. A cross-sectional study was conducted at school with special needs between June-December, 2019. A total of 150 participants were recruited through simple random sampling. Stool samples were examined microscopically by formalin-ether concentration and Ziehl-Neelsen staining technique. Age was analyzed using the Mann-Whitney tests, while the other variables used chi-square tests. Multivariable logistic regression was conducted to identify factors associated with intestinal protozoan infections. The adjusted prevalence ratio with a 95% confidence interval at a 5% level of significance was used to measure the strength of association. Overall, there were 15 children infected by intestinal protozoan among 130 subjects with mean age of participants of 9.83 ± 3.1 years. The intestinal protozoan species were Entamoeba histolytica 7 (5.38%), Giardia lamblia 4 (3.08%), Blastocystis hominis 7 (5.38%) and Iodamoeba butschlii 1 (0.77%). Prevalence of intestinal protozoan infection among children with disabilities in Bantul District, Yogyakarta, Special Region was 11.54%. There were no significant correlations between the risk factors and intestinal protozoan infection among children with disabilities (p>0.05).
Uji Silang Digital sebagai Alternatif Uji Banding Manual Pemeriksaan Mikroskopis Malaria di Kulon Progo Elsa Herdiana Murhandarwati; Anis Fuad; Guntur Budi Herwanto; Rizqiani Amalia Kusumasari; Mahardika Agus Wijayanti; supargiyono supargiyono
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 4, No 1 (2018): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3139.128 KB) | DOI: 10.22146/jpkm.33288

Abstract

Malaria is an infectious disease in tropical countries including Indonesia which causes serious clinical manifestations and even death. Indonesia Ministry of Health targets that malaria is eliminated from this country by 2030. In Java, malaria is targeted to be eliminated by 2015. However, elimination has not been achieved because some areas, including Kulon Progo, Daerah Istimewa Yogyakarta often experiences outbreaks (Kejadian Luar Biasa) in recent years. As microscopic diagnosis is a gold standard diagnosis for malaria, thus accurate microscopic examination is important. Disappearance of malaria cases in malaria pre-elimination areas causes laboratory workers/microscopists are less exposed to experience in identifying malaria. As part of quality assurance, continuous quality control is expected to maintain the accuracy of malaria diagnosis in endemic areas. However, the cross-check sistem as a part of this quality control strategy still has constraints, such as: transportation costs, limited human resources and irregularity. Ibm team of Faculty of Medicine UGM has successfully developed a digital microscopic cross-check sistem. This method is able to bridge the distance and time constraint also transportation costs of slide submissions to be cross-checked to the referral laboratory. Our study partners were microscopists of Kulon Progo Primary Health Centers (Puskesmas) facilitated with digital devices installed in a microscope. Partners were trained to: 1. take microscopic images of suspected malaria parasites and 2. familiarize with digital cross-check sistem. The successful implementation of digital malaria cross-check is expected to improve the accuracy of malaria diagnosis and assist the Government in malaria elimination program especially in Kulon Progo. This system has been registered for Intellectual Property Right no. 091306 obtained from Department of Justice and Human Right of Republic of Indonesia.
In Vitro Antiplasmodial Activity and Cytotoxicity of Active Subfractions of Harmsiopanax aculeatus Leaves Rachel Turalely; Mahardika Agus Wijayanti; Triana Hertiani; Musthofa Mustofa
Indonesian Journal of Pharmacy Vol 31 No 1, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss1pp51

Abstract

Harmsiopanax aculeatus leaves, a medicinal plant with locally named kapur, have been used traditionally to treat malaria in Maluku, Indonesia. However, the scientific information of this plant is still limited. In our previous study, the methanol extract of this plant leaves have been proven to possess in vitro antiplasmodial activity. This study was conducted to evaluate in vitro antiplasmodial activity and cytotoxicity of  subfractions of the plant leaves. Fractionation was performed using a column chromatography with Sephadex LH-20 as the  stationary phase and methanol as the mobile phase. The subfractions obtained were then tested for in vitro antiplasmodial activity on a chloroquine-resistant FCR3 strain of Plasmodium falciparum using a visual method. Cytotoxicity was evaluated by using MTT assay. The in vitro antiplasmodial activity and cytotoxicity were expressed as IC50, calculated using probit analysis with SPSS 16 for windows. The results showed that the four subfractions tested have a high antiplasmodial activity with IC50 values of 0.09; 0.18; 0.01; and 0.77 µg.mL-1, respectively. In addition, these subfractions had IC50 values of >400 µg.mL-1 against Vero cells indicating that they were non-toxic. In conclusion, the subfractions of H. aculeatus leaves are very active and selective against P. falciparum. Further study will be conducted to isolate the active compounds.
SINTESIS DAN UJI AKTIVITAS SENYAWA (1)-N-(n-BUTIL)- DAN (1)-N-(t-BUTIL)-1,10-FENANTROLINIUM SEBAGAI SENYAWA POTENSIAL ANTIMALARIA BARU Ruslin Hadanu; Sabirin Mastjeh; Mustafa Mustafa; Eti Nurwening Sholikhah; Mahardika Agus Wijayanti
Molluca Journal of Chemistry Education (MJoCE) Vol 1 No 1 (2011): Molluca Journal of Chemistry Education (MJoCE)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/MJoCEvol1iss1pp45-53

Abstract

The synthesis of (1)-N-(n- buthyl)- dan (1)-N-(t-buthyl)-1,10-phenanthrolinium with 1,10-phenanthroline monohydrate as starting material through two steps has been carried out. The first step of reaction is chlorination and bromination of n-buthyl-alcohol/t-buthyl alcohol using HCl and HBr, respectively. The result of reaction is nbuthyl bromide 2 (colourless liquid, 70.92%) and t-buthyl chloride 4 (colourless liquid, 92.36%), respectively. The second step of reaction is alkylation of 1,10-phenanthroline 5 using n-buthyl bromide and t-buthyl chloride reagents that its was refluxed for 21 and 23 h, respectively. The results of reaction are (1)-N-(n- buthyl)-1,10fenantrolinium bromida 6 and (1)-n-(t-buthyl)-1,10-fenantrolinium chloride 7 in yield from 84.70% and 78.16%, respectively. The results of testing in in vitro antiplasmodial activity at chloroquine-resistant P. falciparum FCR3 strain to (1)-N-(n-buthyl)- and (1)-N-(t-buthyl)-1,10- phenanthrolinium obtained that (1)-N-(n-buthyl)-1,10phenanthrolinium bromide 6 has higher antimalarial activity (IC50 : 0.03±0.01 µM) than antimalarial activity of (1)-n-(t-buthyl)-1,10-phenanthrolinium chloride 7 (IC50 : 2.09±0.08 µM). While, the results of testing in in vitro antiplasmodial activity at chloroquine-resistant P. falciparum D10 strain to (1)-N-(n-buthyl)- and (1)-N-(t-buthyl)1,10-f phenanthrolinium obtained that (1)-N-(n-butil)-1,10- phenanthrolinium bromide 6 has higher antimalarial activity (IC50 : 1.40±0.82 µM) than antimalarial activity of (1)-n-(t-buthyl)-1,10- phenanthrolinium chloride 7 (IC50 : 2.24±0.05 µM
SINTESIS DAN UJI AKTIVITAS ANTIMALARIA SENYAWA (1)-N-BENZIL-1,10- FENANTROLINIUM BROMIDA Ruslin Hadanu; Sabirin Mastjeh; Jumina Jumina; Mustafa Mustafa; Eti Nurwening Sholikhah; Mahardika Agus Wijayanti
Molluca Journal of Chemistry Education (MJoCE) Vol 1 No 2 (2011): Molluca Journal of Chemistry Education (MJoCE)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/MJoCEvol1iss2pp92-102

Abstract

A synthetic methods was employed to prepare (1)-N-benzyl)-1,10-phenanthroliniumbromide 4 from 1,10phenanthroline 3 and benzylchloride 1 through substitution and alkylation reactions. The compound (1)-Nbenzyl)-1,10-phenanthroliniumbromide 4 was tested through antiplasmodial test. The benzylbromide 2 was synthesized through substitution reaction of benzylchloride 1 and NaBr which has a yield of 74.25%. The (1)-N-benzyl)-1,10-phenanthroliniumbromide 4 was synthesized from 1,10-phenanthroline 3 using benzylbromide 2 reagents which refluxed for 8 hours with 84.04% yields. The results of testing in in vitro antiplasmodial activity at chloroquine-resistant Plasmodium falciparum FCR3 strain to (1)-N-benzyl)-1,10- phenanthroliniumbromide 4 has high antimalarial activity (IC50 : 0.10±0.04 µM)
Antiplasmodial Activity, Cytotoxicity, and Active Compounds Analysis of Active Fraction of Harmsiopanax aculeatus Leaves from Maluku, Indonesia Turalely, Rachel; Mustofa; Hertiani, Triana; Wijayanti, Mahardika Agus
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.879-895

Abstract

The leaves of the H. aculeatus plant are known as a traditional antimalarial medicinal plant in Maluku. Several studies have reported on the leaf activity of this plant, but studies on in vitro and in vivo antiplasmodial activity and the compounds in the active fraction (F7) of H.aculeatus leaf have not been reported. This study aims to determine the antiplasmodial activity of the F7 of H. aculeatus leaves and to analyze the compounds contained therein. In vitro, antiplasmodial activity was tested on Plasmodium falciparum strain FCR3 using a microscopic method. A cytotoxicity test was performed on Vero cells using an MTT assay. In vivo, the antiplasmodial activity of F7 was carried out using the 4-day suppressive test method by treating Swiss mice infected with P. berghei. Analysis of the compounds in an F7 was done using spray reagent and UV Vis DAD and HPLC DAD-MS with a UV detector. The results showed that the F7 was very active in vitro (IC50 of 0.7 µg.ml-1) and in vivo (ED50 of 2.49 mg.kg BW-1.d-1) also selectively (SI of 8159.94) inhibited the growth of Plasmodium. The F7 contains a group of essential oils, triterpenoids, phenolic compounds, and flavonoids after being analyzed using spray reagents. Based on the analysis results, five compounds were identified: fomoxanthone, cyclopentene, microspherone, indole 3-carbaldehyde, and naamine. In addition, seven compounds were not identified.
Co-Authors Anis Fuad Azmi, Fahriana E. Elsa Herdiana Murhandarwati Elsa Herdiana M Eti Nurwening Sholikhah Herwanto, Guntur Budi I Nengah Kapti I Wayan Sudiarta Jumina Jumina Kusumasari, Rizqiani Amalia Mustafa Mustafa Musthofa Mustofa Mustofa Mustofa Ni Wayan Winianti Putu Sutisna Rachel Turalely Rachel Turalely Ruslin Hadanu Sabirin Mastjeh SANTOSO SANTOSO Selvi Marcellia Sitti Rahmah Umniyati Supargiyono Supargiyono supargiyono supargiyono Supargiyono Supargiyono Triana Hertiani Zeth Robeth Felle