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Use of pregnant mareâs sera gonadotropin (PMSG) in media in vitro maturation of cow oocytes Udin, Zaituni; ., Jaswandi; Afriani, Tinda; E, Leonardo
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

It is known that hormone addition in media helps in vitro maturation of oocyte. This research was aimmed to determine the effect of PMSG in media to maturation rate and nucleous developvement of cow oocyte. Ovaries were obtainned from local slaughterhouse. The media used for in vitro maturation of oocyte was TCM- 199 and the treatment was 3 levels of PMSG: 0, 10 and 20 mg/ml. Result of this research showed that the dose of PMSG in maturation media was significantly affected (P<0.05)Â nucleolus development of oocytes and maturation rate. The average of germinal vesicle (GV) stage in 3 levels of PMSG 0, 10 and 20 mg/ml were 38.33; 12.64 and 9.64%, respectivelly. There was no germinal vesicle breakdown (GVBD) found in 3 levels of PMSG addition. The nucleous development of metaphaseâI (M-I) were 7.64; 20.2 and 22.00%, but the average of maturation rate (M-II)Â was 16.32; 48.10 and 35.34% for 3 levels of PMSG: 0, 10 and 20 mg/ml, respectivelly. It is concluded that 10 mg/ml PMSG in media of in vitro maturation resuls in the highest maturation rate of cow oocyte. Key Words: Oocyte, Maturation, In vitro, Hormone, PMSG

Use of pregnant mare’s sera gonadotropin (PMSG) in media in vitro maturation of cow oocytes Zaituni Udin; Jaswandi .; Tinda Afriani; Leonardo E
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

It is known that hormone addition in media helps in vitro maturation of oocyte. This research was aimmed to determine the effect of PMSG in media to maturation rate and nucleous developvement of cow oocyte. Ovaries were obtainned from local slaughterhouse. The media used for in vitro maturation of oocyte was TCM- 199 and the treatment was 3 levels of PMSG: 0, 10 and 20 mg/ml. Result of this research showed that the dose of PMSG in maturation media was significantly affected (P<0.05) nucleolus development of oocytes and maturation rate. The average of germinal vesicle (GV) stage in 3 levels of PMSG 0, 10 and 20 mg/ml were 38.33; 12.64 and 9.64%, respectivelly. There was no germinal vesicle breakdown (GVBD) found in 3 levels of PMSG addition. The nucleous development of metaphase–I (M-I) were 7.64; 20.2 and 22.00%, but the average of maturation rate (M-II) was 16.32; 48.10 and 35.34% for 3 levels of PMSG: 0, 10 and 20 mg/ml, respectivelly. It is concluded that 10 mg/ml PMSG in media of in vitro maturation resuls in the highest maturation rate of cow oocyte. Key Words: Oocyte, Maturation, In vitro, Hormone, PMSG

Pengaruh Kawin Pertama Pascapartum Sapi Potong terhadap Angka Kebuntingan di Kodya Padang Zaituni Udin
Buletin Peternakan Vol 29, No 4 (2005): Buletin Peternakan Vol. 29 (4) November 2005
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v29i4.1183

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Waktu dan Kemerahan Vulva Saat Inseminasi Buatan Merupakan Faktor Penentu Angka Kebuntingan Sapi di Sumatera Barat (TIME AND REDDISH SIGN OF VULVA DURINGARTIFICIAL INSEMINATION AS A DETERMINANT FACTORS ON CONCEPTION RATE OF COW IN WEST SUMATERA) Zaituni Udin; Ferdinal Rahim; Hendri Hendri; Yulia Yellita
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.181 KB)

An experiment was conducted to evaluate determinant factors on conception rate of local and crossbreedcow at West Sumatera. There were 162 cows of four AI center at parity-0 to 4TH parity (P-4), inseminatedusing frozen semen of four different bulls (simental,brahman, limousine and Bali cattle). All fed similarityat four AI centers and location to observation of sign of estrus in high land and low land in West Sumatera.All data were analyzed using analyzed of variance in Randomized Block Design (RBD) in factorial as afactor A was time of inseminated; parity;sign of vulva,breed and two location as a replication. Resultsofthis study showed that there were no interaction between time of insemination, breed, parity and thereddish sign of vulva (P>0.05) on conception rate. On the contrary,there were significantly different(P>0.05) on conception rate by time of insemination. The highest conception rate wasfound at the end ofestrus 13–18 h (68.91%),local cow (71.82%), P-3 (67.19%) and reddish sign of vulva (74.25%). It wasconcluded that the effects,both inseminationtime and reddish sign of vulva might be the important factorto get the highest conception rate of the cows.

Suplementasi Mineral Lokal untuk Perbaikan Nutrisi dan Reproduksi Sapi Peranakan Simmental Dara pada Peternakan Rakyat di Jorong Sibaladuang, Kabupaten Limapuluh Kota . Khalil; . Andri; Zaituni Udin
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 5 No. 3 (2019): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.5.3.202-209

This community service was aimed to evaluate beneficial effect and constrains of mineral supplementation onfeed of Simmental heifers that be raised by smallholders. Mineral were formulated by using locally available materials (rock flour, limestone, fresh water oyster shell meal) and prepared in loose and lick-block forms. The local mineral formulas were fed to 24 Simmental heifers raised by 17 smallholders for 14 weeks in four treatments: P0 (no supplementation, control), P1 (supplemented with commercial mineral premix), P2 (supplemented with local mineral feed in loose meal), and P3 (supplemented with local mineral feed in form). Each treatment consisted of 6 heifers as replication. Parameters measured included: blood minerals (Ca, P, Mg) and blood hematology (hemoglobin (Hb), mean corpuscular Hb concentration (MCHC), total red blood cell (RBC), white blood cell (WBC), hematocrit concentration (HCT)), total protein, and estrus onset. Results showed that heifers supplemented with local minerals (P2 and P3) were detected earlier estrus onset than the control (P0) and that supplemented with commercial mineral premix (P1). Local mineral supplementation gave also positive effect on blood mineral, protein and hematological profiles, even though there were no statistically difference. It was concluded that mineral supplementation gave positive effect of reproduction performances and nutritional status of Simmental heifers. Local mineral offered in lick-block had better effect than that in loose form.

Pengaruh Pemberian Capriglandin dan Lutalyse Terhadap Service Perconception, Conception Rate dan Ovarium pada Sapi Simmental Bahagia, Sari; Hendri, Hendri; Udin, Zaituni
Journal of Livestock and Animal Health Vol. 5 No. 2 (2022): August
Publisher : Politeknik Pertanian Negeri Payakumbuh

Penelitian ini bertujuan mengetahui pengaruh pemberian PGF2 (Capriglandin dan Lutalyse) terhadap service perconception, conception rate dan ovarium, folikel, dan corpus luteum pada induk sapi simmental. Penelitian menggunakan 24 ekor induk sapi Simmental yang tidak bunting di BPTUHPT Padang Mengatas, Sumatera Barat dengan kriteria tidak estrus, siklus estrus normal dan tidak mengalami gangguan reproduksi. Metode eksperimen dengan Rancangan Acak Lengkap Pola Faktorial 2 x 2 x 6 bila terdapat hasil berbeda nyata maka dilanjutkan uji lanjutan DMRT. Faktor A adalah preparat hormon prostaglandin (Capriglandin dan Lutalyse). Faktor B adalah dosis (3 ml dan 5 ml). Perlakuan A1B1 = Capriglandin 3 ml; A1B2 = Capriglandin 5 ml; A2B1 = Lutalyse 3 ml; dan A2B2 = Lutalyse 5 ml yang masing-masing terdapat enam ulangan. Baik Capriglandin dan Lutalyse diberikan secara single dosis intra muskuler. Parameter yang diukur service perconception, conception rate dan ukuran diameter panjang ovarium. Hasil analisis antara preparat hormon Capriglandin dan lutalyse terhadap jumlah dosis 3 ml dan 5 ml menunjukan hasil berbeda nyata (P kecil dari 0.05) terhadap conception rate dan ovarium. Hasil analisis tidak berbeda nyata (P besar dari 0,05) terhadap service perconception. Prostaglandin dengan zat aktif dinoprost tromethamine hanya efektif bila ada CL fungsional, yaitu antara hari ke-7 sampai hari ke-18 siklus estrus. Penggunaan terbaik pada pemberian Capriglandin dengan dosis 5 ml.

QUALITY DIFFERENCES OF BOER LIQUID SEMEN DURING STORAGE WITH ADDITION SWEETORANGEESSENTIAL OIL IN TRIS YOLK AND GENTAMICIN EXTENDER Sitepu, Sukma Aditya; Udin, Zaituni; Jaswandi, Jaswandi; Hendri, Hendri
Journal of Community Research and Service Vol 1, No 2: July 2017
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jcrs.v1i2.9341

AbstractThe purpose of this study was to know the quality of Boer liquid semen during storage by adding sweet orange essential oil and gentamicin into the tris yolkextender.The semenlongevity test was carried out by storing semen in a closed tube at room temperature and refrigerator, and evaluated motility and Viability every 3 hours at room temperature and 12 hours in refrigerator. The results showed that the characteristics of the liquid semen, with the addition of 1% of essential oils showed percentage of motility and Viability significantly higher than 0.5% and without the addition of essential oil, either stored at room temperature or in the refrigerator. This may be related to the content of essential oils of sweet orange peel containing flavonoids and antibacterials that are capable of maintaining liquid semen quality of Boer Goat.In addition, gentamicin contains antibacterials capable of suppressing the growth of bacteria which can damage and durabilitythe spermatozoa.Keywords: Boer Goat,Gentamicin, Liquid semen, Sweet orange

QUALITY DIFFERENCES OF BOER LIQUID SEMEN DURING STORAGE WITH ADDITION SWEETORANGEESSENTIAL OIL IN TRIS YOLK AND GENTAMICIN EXTENDER Sitepu, Sukma Aditya; Udin, Zaituni; Jaswandi, Jaswandi; Hendri, Hendri
Journal of Community Research and Service Vol. 1 No. 2: July 2017
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jcrs.v1i2.9341

AbstractThe purpose of this study was to know the quality of Boer liquid semen during storage by adding sweet orange essential oil and gentamicin into the tris yolkextender.The semenlongevity test was carried out by storing semen in a closed tube at room temperature and refrigerator, and evaluated motility and Viability every 3 hours at room temperature and 12 hours in refrigerator. The results showed that the characteristics of the liquid semen, with the addition of 1% of essential oils showed percentage of motility and Viability significantly higher than 0.5% and without the addition of essential oil, either stored at room temperature or in the refrigerator. This may be related to the content of essential oils of sweet orange peel containing flavonoids and antibacterials that are capable of maintaining liquid semen quality of Boer Goat.In addition, gentamicin contains antibacterials capable of suppressing the growth of bacteria which can damage and durabilitythe spermatozoa.Keywords: Boer Goat,Gentamicin, Liquid semen, Sweet orange

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