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All Journal JURNAL GALUNG TROPIKA Jurnal Medik Veteriner
Yulia Yellita
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Efektifitas Herba Ciplukan (Physalis angulata L.) Sebagai Imunostimulan Kekebalan Unggas Setelah Uji Tantang Virus Newcastle Desease Nurhasanah, N; Yellita, Yulia; Sabrina, S; Ananda, A
Jurnal Galung Tropika Vol 12 No 2 (2023)
Publisher : Fakultas Pertanian, Peternakan dan Perikanan Universitas Muhammadiyah Parepare

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v12i2.1074

Abstract

This research aims to determine the effectiveness of ciplukan herb (Physalis angulata L) in extract and powder preparations as an immunostimulant against the immunity of broilers who have received vaccination and tested against the Newcastle disease virus. The materials used were 96 DOC with an average weight of 35-40 g, ciplukan herb from Luhak Nan Duo, the velogenic strain of Newcastle disease virus from Bvet Lampung, and the commercial vaccine ND Lasota. The study used a completely randomized design (CRD) with 8 treatments and 3 replications. The ciplukan herb was given for 3 days with concentrations: K(-) (without vaccine without ciplukan), K+ (vaccine only), E3 (ciplukan extract 0.35mg/10ml/head + vaccine), E2 (ciplukan extract 0.25mg/head 10ml/head + vaccine), E1 (0.15mg/10ml/head ciplukan extract + vaccine), S3 (17.5mg ciplukan powder/head + vaccine), S2 (12.5mg ciplukan powder/head + vaccine) and S1 (ciplukan powder 7.5mg/head + vaccine). The parameters observed in this study were antibody titer, morbidity, and mortality. The study showed that the administration of ciplukan herb positively affected low antibody titers through the efficiency of cellular defense mechanisms. This herb can also reduce chicken mortality and morbidity after challenge tests against the Newcastle disease virus.
Prevalence and molecular characterization of Babesia and Theileria species in cattle from West Sumatra, Indonesia Cahyaningsih, Umi; Yellita, Yulia; Putra, Eliyus; Roza; Satrija, Fadjar; Arif, Ridi; Inarsih, Dwi; Santosa, Budi; Nugraha, Arifin Budiman
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.41-51

Abstract

This epidemiological investigation conducted in three regencies of West Sumatra, Indonesia, aimed to examine the molecular prevalence and distribution of Babesia bigemina, Babesia bovis, and Theileria orientalis. A cross-sectional study employing a convenience sampling technique included 151 cattle blood samples. These samples were collected from three regencies (Agam, Limapuluh Kota, and Tanah Datar) between January 2023 and February 2024. Blood samples were examined using polymerase chain reaction (PCR) coupled with sequencing, targeting the cytochrome b gene for B. bigemina and B. bovis, and the 18S rRNA gene for Theileria spp. A total of 151 cattle blood samples from West Sumatra were examined for hemoprotozoan infections using both microscopic and molecular approaches. Based on blood smear examination, 23 (15.2%) and 118 (78.1%) samples were positive for Babesia spp. and Theileria spp., respectively. Subsequent nested PCR analysis confirmed all Babesia-positive samples as B. bigemina, while B. bovis was not detected. In contrast, 131 (86.7%) samples were molecularly identified as T. orientalis. Phylogenetic analysis of the cytochrome b gene revealed three distinct clades of B. bigemina, indicating genetic diversity likely shaped by geographical distribution, vector ecology, or host adaptation. These findings demonstrate that T. orientalis is the predominant hemoprotozoan agent in West Sumatra, while the genetic variability of B. bigemina underscores the complexity of its epidemiology. Together, these results provide critical insights for improving surveillance and guiding the formulation of effective hemoparasite management strategies in the tropical Indonesian archipelago.
Co-Authors Ananda Ananda Budi Santosa Budi Santosa Inarsih, Dwi N Nurhasanah Nugraha, Arifin Budiman Putra, Eliyus Ridi Arif Roza Sabrina, S Umi Cahyaningsih