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All Journal Prosiding Seminar Nasional MIPA Indonesia Chimica Acta Chemistry Indonesian Journal of Chemical Research
Hanapi Usman
Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Hasanuddin
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science

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Isolasi, Karakterisasi, dan Uji Bioaktivitas Metabolit Sekunder dari Hydroid Aglaophenia cupressina Lamoureoux Sebagai Bahan Dasar Antimikroba Johannes, Eva; Usman, Hanapi; Ahmad, Ahyar
Indonesia Chimica Acta Volume 2 No 1 - June 2009
Publisher : Indonesia Chimica Acta

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Abstract

Isolation, structure elucidation, activity test against Salmonella thypi, Staphylococcus aureus of secondary metabolite from hydroid Aglaophenia cupressina Lamoureoux, were conducted. This study was aimed to identify and isolate the compound with antibacterial properties of hydroid Aglaophenia crupessina Lamoureoux. This study used stepwise method with the following sequences: extraction, isolation, purification, structure elucidation, and bioactivity test. Study results indicated three compounds found: (1)carboxylate acid compound: hexadecanoic acid and has antibacterial properties; (2) alkaloidcompound which was considered as a new compound: aglaounhas and has antimicrobialproperties; (3) steroid compound: β-sitosterol with no antimicrobial properties.Keywords: Isolation, characterization, secondary metabolite, Aglaophenia cupressina, Antibacterial.
PEMANFAATAN MEDIUM ARS-CHAT PADA PRODUKSI BIOMASSA FITOPLANKTON LAUT YANG POTENSIAL SEBAGAI BAHAN BAKU BIOFUEL JENIS BIOETANOL Kasim, Syahruddin; Sjahrul, M; Usman, Hanapi
Prosiding Seminar Nasional MIPA 2013: PROSIDING SEMINAR NASIONAL MIPA UNDIKSHA 2013
Publisher : Prosiding Seminar Nasional MIPA

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Abstrak   Tujuan utama yang ingin dicapai pada penelitian ini adalah mengidentifikasi sejumlah fitoplankton laut untuk produksi biomassa dan penentuan kadar karbohidratnya. Fitoplankton laut dikulturkan dalam medium “Ars-chat”. Dalam media kultur massal dilakukan isolasi dan identifikasi jenis fitoplankton laut berdasarkan ukuran besaran dan cirri-ciri tertentu. Juga dalam media dilakukan pengukuran terhadap suhu, salinitas dan pH larutan. Kepadatan fitoplankton terpilih, ditentukan berat biomassanya dengan metoda gravimetri dan dilakukan analisis kandungan karbohidratnya dengan metode penentuan gula reduksi dengan cara Luff Schoorl. Hasil isolasi diperoleh tujuh jenis fitoplankton laut yaitu : Chlorella sp., Dunaliella sp., Tetraselmis chuii, Spirulina sp, Chaetoceros calcitrans, C. gracilis, and C. Isocrysis galbana. Kandungan Biomassa  tertinggi 0,39 g/L pada Chlorella sp. dan terendah 0,19 g/L pada C. Isocrysis galbana. Kadar karbohidrat juga bervariasi, yang tertinggi 32,97% dijumpai pada Dunaliella sp. dan terendah 6,17% pada C.gracilis.  
Oleanan Derivate Compounds From Melochia umbellata (Houtt) Stapf var. Degrabrata K Bark And Their Bioactivity Usman, Usman; Soekamto, Nunuk H.; Usman, Hanapi; Ahmad, Ahyar
Indonesian Journal of Chemical Research Vol 2 No 1 (2014): Edisi Bulan Juli (Edition For July)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2014.2-usm

Abstract

Oleanan derivated 3-acetyl-12-oleanen-28-oat has been isolated from the n-hexane fraction of M. umbellata(Houtt) Stapf var. degrabrata K. bark. The molecular structure were determined by IR spectroscopy, 1D and 2DNMR (1H, 13C, DEPT, COSY, HMQC and HMBC) data analysis. Bioactivity tested results indicated that thecompound was toxic to A. salina with LC50 value was 361.93 mg/mL. At the concentration of 1000 mg/mL 3acetyl-12-oleanen-28-oat gave the highest inhibition to bacteria B. subtilis and the fungus C. albicans with the inhibited zone diameter was 15.8 mm and 15.2 mm, res.
Co-Authors Ahyar Ahmad Eva Johannes M Sjahrul Nunuk H. Soekamto Syahruddin Kasim Usman Usman