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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Pelita Perkebunan Journal of Degraded and Mining Lands Management Jurnal Ilmu Kelautan Spermonde BIOMA : Jurnal Biologi Makassar Indonesia Chimica Acta International Journal of Applied Biology Jurnal Ilmu Alam dan Lingkungan
Eva Johannes
Biology Dept. MIPA Universitas Hasanuddin, Makassar 90245
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Veterinary

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AKTIVITAS UJI ANTIMITOTIK SENYAWA ASAM HEKSADEKANOAT ISOLAT DARI HYDROID Aglaophenia cupressina Lamoureoux PADA CLEAVAGE BULU BABI Tripneustes gratilla Linn Sjafaraenan, Sjafaraenan; Johannes, Eva
BIOMA : Jurnal Biologi Makassar Vol 1, No 1 (2016)
Publisher : Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan alam, Universitas Hasanuddin

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Abstract

AbstrakStudi tentang aktivitas antimitotik senyawa asam heksadekanoat isolat dari hydroid Aglaopehenia cupressina Lamoureoux pada cleavage bulu babi Tripneustes gratilla Linn. Penelitian ini bertujuan untuk mengetahui pengaruh senyawa bioaktif asam heksadekanoat dari hydroid Aglaopehenia cupressina Lamoureoux pada cleavage bulu babi Tripneustes gratilla Linn. Metode yang digunakan adalah metode uji aktivitas antimitotik senyawa asam heksadekanoat pada cleavage bulu babi Tripneustes gratilla Linn. pada konsentrasi 1, 10, dan 100 µg/ml dengan kontrol positif vinkristin pada konsentrasi 0.01, 0.1, dan 1 µg/ml setelah fertilisasi penghambatan tertinggi terjadi pada konsentrasi 100 µg/ml yaitu 66,66 %. Kemudian setelah dihitung dengan analisis probit, asam heksadekanoat memiliki IC50 sebesar 22,076 µg/ml yang memiliki kesamaan sifat antimitotik dengan vinkristin yang memiliki IC 50 sebesar 0,219 µg/ml. Sehingga asam heksadekanoat isolat dari hydroid Aglaopehenia cupressina Lamoureoux berpotensi untuk dijadikan bahan dasar anti kanker
Isolasi, Karakterisasi, dan Uji Bioaktivitas Metabolit Sekunder dari Hydroid Aglaophenia cupressina Lamoureoux Sebagai Bahan Dasar Antimikroba Johannes, Eva; Usman, Hanapi; Ahmad, Ahyar
Indonesia Chimica Acta Volume 2 No 1 - June 2009
Publisher : Indonesia Chimica Acta

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Isolation, structure elucidation, activity test against Salmonella thypi, Staphylococcus aureus of secondary metabolite from hydroid Aglaophenia cupressina Lamoureoux, were conducted. This study was aimed to identify and isolate the compound with antibacterial properties of hydroid Aglaophenia crupessina Lamoureoux. This study used stepwise method with the following sequences: extraction, isolation, purification, structure elucidation, and bioactivity test. Study results indicated three compounds found: (1)carboxylate acid compound: hexadecanoic acid and has antibacterial properties; (2) alkaloidcompound which was considered as a new compound: aglaounhas and has antimicrobialproperties; (3) steroid compound: β-sitosterol with no antimicrobial properties.Keywords: Isolation, characterization, secondary metabolite, Aglaophenia cupressina, Antibacterial.
Combining biochar with sediment in the treatment for the effectiveness of sulfate and heavy metal Pb reduction of acid mine drainage Fahruddin, Fahruddin; Syahri, Yolanda Fitria; Fauziah, St.; Samawi, Muhammad Farid; Johannes, Eva; Tambaru, Elis; Tuwo, Mustika; Abdullah, As’adi
Journal of Degraded and Mining Lands Management Vol. 11 No. 4 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.114.6329

Abstract

The increasing mining activities have led to the problem of acid mine drainage (AMD) pollution. A method that combines biochar treatment as an adsorbent with wetland sediment treatment as a source of sulfate-reducing bacteria is used to address AMD effectively. This research aimed to determine the ability of biochar in combination with wetland sediment treatment to reduce sulfate and heavy metal content in acid mine drainage wastewater. This research was conducted on a laboratory scale in an AMD wastewater treatment reactor with the following treatments of biochar mixed with wetland sediment. Observations included sulfate content, pH, and heavy metal content. Scanning electron microscope (SEM) analysis was also performed on the biochar. SEM observations revealed the presence of small, dense, and irregularly shaped pores on the surface of the biochar. The results on day 30 showed that biochar mixed with wetland sediment was able to reduce sulfate concentration by 74.19% and reduced Pb by 73.79%, compared with treatment sediment only to 64.81% sulfate concentration and reduced Pb by 53.85%, treatment biochar only had reduced sulfate of 46.90% and reduced Pb by 58.67% and control 1.79% sulfate concentration and reduced Pb by 1.87%.
Pengaruh Edible Coating Berbasis Pati Ubi Kayu Manihot esculenta Crantz dengan Penambahan Ekstrak Jahe Merah Zingiber officinale var. Rubrum Terhadap Umur Simpan Buah Potong Wortel Daucus carota L. Johannes, Eva; Tuwo, Mustika
Jurnal Ilmu Alam dan Lingkungan Vol. 15 No. 1 (2024): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

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Edible coating menjadi salah satu usaha yang dikembangkan untuk mengatasi busuk lunak pada buah potong seperti pada buah dan sayuran. Penelitian ini bertujuan mengkaji pengaruh edible coating dengan penambahan senyawa bioaktif yang bersifat alami terhadap umur simpan wortel Daucus carota L. Metode yang digunakan untuk uji antibakteri menggunakan metode difusi agar, uji antioksidan menggunakan metode DPPH, perendaman atau pengemasan menggunakan metode edible coating. Untuk pelapisan adalah edible coating berbasis pati dengan penambahan ekstrak jahe merah Zingiber officinale var. Rubrum sebagai senyawa bioaktif yang memiliki sifat antibakteri dan antioksidan. Perendaman sampel dengan bahan akuades, larutkan CMC (Carboxy Methyl Cellulose) 0.4% dan gliserol 5%, penambahan ekstrak jahe merah 0.1%. Perlakuan terdiri dari kontrol (tanpa perlakuan), pelapisan dengan edible coating, dan edible coating dengan penambahan ekstrak jahe merah, lama penyimpanan (3 hari, 6 hari, 9 hari, 12 hari). Selama masa simpan dilakukan pengujian susut bobot dan tekstur. Hasil uji edible coating dengan penambahan ekstrak jahe bersifat bakterisida terhadap bakteri Erwinia carotovora pv. carotovora, memiliki sifat antioksidan yang kuat dengan nilai IC50 sebesar 72.40 ppm. Memiliki susut bobot terendah pada perlakuan edible coating dengan penambahan ekstrak jahe sebesar 4.42% pada hari ke 12. Tekstur yang terbaik pada perlakuan edible coating dengan penambahan ekstrak jahe sebesar 1.53 N pada hari ke 12. Hasil tersebut menunjukkan edible coating dengan penambahan ekstrak jahe merah 0.5% mampu meningkatkan umur simpan wortel selama 12 hari pada suhu kamar.
Uji Awal Aktivitas Sitotoksik Ekstrak Benalu Jati Dendropthoe pentandra (L.) Miq. Sebagai Kandidat Antikanker Menggunakan Metode BSLT Johannes, Eva; Sjafaraenan, Sjafaraenan; Tuwo, Mustika
Jurnal Ilmu Alam dan Lingkungan Vol. 16 No. 1 (2025): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

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Benalu Dendrophthoe pentandra (L.) Miq. yang tumbuh pada pohon jati dikenal dalam pengobatan tradisional memiliki potensi sebagai antikanker. Penelitian ini bertujuan untuk mengevaluasi aktivitas sitotoksik dari ekstrak etanol daun benalu jati sebagai kandidat antikanker dengan menggunakan metode Brine Shrimp Lethality Test (BSLT). Daun benalu dikeringkan, diekstraksi menggunakan pelarut etanol 96%, dan diuji terhadap larva Artemia salina untuk menilai tingkat toksisitas awal. Hasil pengujian menunjukkan bahwa ekstrak memiliki nilai LC₅₀ sebesar XXX µg/mL, yang menunjukkan potensi toksik yang signifikan terhadap larva uji. Semakin rendah nilai LC₅₀, semakin tinggi potensi sitotoksik yang dimiliki oleh ekstrak tersebut. Uji BSLT digunakan sebagai pendekatan awal untuk menyaring senyawa bioaktif yang berpotensi sebagai antikanker sebelum dilakukan pengujian lebih lanjut pada sel kanker manusia. Penelitian ini mendukung pemanfaatan D. pentandra sebagai sumber kandidat senyawa alami antikanker dan menjadi dasar untuk studi lanjutan, termasuk isolasi senyawa aktif dan uji in vitro terhadap sel kanker spesifik. Hasil ini juga menegaskan pentingnya eksplorasi tumbuhan parasit yang sering diabaikan sebagai sumber daya bioaktif yang potensial.
Antibacterial and Antifungal of β-sitosterol Isolated from Hydroid Aglaophenia cupressina Lamoureoux Against Xanthomonas campestris and Fusarium oxysporum Johannes, Eva; Manguntungi, Baso; Tuwo, Mustika; Litaay, Magdalena; Mustopa, Apon Zaenal; Vanggy, Leggina Rezzy
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 30, No 2 (2025): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.30.2.192-202

Abstract

This study aimed to isolate and characterize β-sitosterol from the marine hydroid Aglaophenia cupressina Lamoureoux and evaluate its antibacterial and antifungal activities against Xanthomonas campestris and Fusarium oxysporum. β-Sitosterol was extracted, purified, and identified using spectroscopic techniques, including infrared and nuclear magnetic resonance spectroscopy. Antimicrobial activity was assessed through agar diffusion method to determine its inhibitory effects on bacterial and fungal growth at varying concentrations. β-sitosterol is a crystalline compound, with a melting point of 138-139°C, consistent with the reported range for pure β-sitosterol, indicating high purity. Infrared (IR) spectroscopy revealed key functional groups, including a hydroxyl group at 3433 cm⁻¹, C-O stretching at 1050 cm⁻¹, and aliphatic hydrocarbon chain vibrations at 2956, 2938, and 2869 cm⁻¹. The C=C stretching at 1634 cm⁻¹ and C-H bending at 1465 cm⁻¹ confirmed its unsaturated sterol structure. ¹H NMR spectroscopy further confirmed the structure with characteristic methyl and olefinic proton signals. The antibacterial activity of β-sitosterol against Xanthomonas campestris showed a concentration- and time-dependent effect, with the highest efficacy observed at 60 ppm, demonstrating potential as a natural antibacterial agent. Additionally, its antifungal activity against Fusarium oxysporum revealed both fungistatic and fungicidal effects, with lower concentrations exhibiting fungistatic behavior and higher concentrations displaying fungicidal activity, thereby offering versatility for both fungal inhibition and eradication. This dual action, combined with its well-characterized molecular structure, positions β-sitosterol as a promising candidate for further development as an antimicrobial compound. The findings underscore the accuracy of the identification process and highlight β-sitosterol's potential in pharmaceutical and agricultural applications, particularly in combating bacterial and fungal infections.
Callus formation of Coffea canephora induced with 2,4 dichlorophenoxyacetic acid and tomato extract supplements Fahira, Nurul Rifqah; Latunra, Andi Ilham; Johannes, Eva
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 39 No. 3 (2023)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v39i3.565

Abstract

Robusta coffee (Coffea canephora) is Indonesia's essential leading trade commodity. Robusta coffee is preferred for its bitter taste. However, robusta coffee productivity decreased due to bad agriculture practice. Tissue culture is an alternative to cultivation with various advantages. The plant growth regulators (PGRs) influence tissue culture’s success. Due to its stability, 2,4 dichlorophenoxyacetic acid has become a common synthetic PGRs. PGRs can be obtained from natural ingredients, such as tomatoes. This study aimed to analyze the effect of 2,4-dichlorophenoxyacetic acid and tomato extract addition and its appropriate concentration for callus induction of robusta coffee in vitro. This research was conducted at the Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University. This study used a Complete Randomized Design with two factors. The first factor was the concentration of tomato extract (0%; 7.5%; 10%; and 12.5%). Meanwhile, the second factor was the concentration of 2,4 dichlorophenoxyacetic acid (0 ppm; 1 ppm; 2 ppm; and 3 ppm). Observation parameters include the percentage of callus formation, callus growing time, callus fresh weight, callus color, and callus texture. The quantitative data were analyzed by the Kruskal-Wallis test followed by the Mann-Whitney Test to compare the effect of each treatment. The results showed that among other treatment combinations, treatment with the addition of 2 ppm 2,4-D and 10% tomato extract (T2D2) and treatment with the addition of 3 ppm 2,4-D and 10% tomato extract (T2D3) had the best effect in inducing the callus of robusta coffee.
Cytotoxic Activity of Bioactve Compounds from Hydroid Aglophenia cupressina Lamoureoux against Hela Tumor Cells Sjafaraenan, Sjafaraenan; Johannes, Eva; Herwin, Herwin
International Journal of Applied Biology Vol. 8 No. 1 (2024): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Hydroids are marine invertebrates living on sponges, which belong to the phylum Coelenterata. They also contain several chemical compounds, including alkaloids, steroids, terpenoids, and histamine, which can be used as medicinal raw materials, such as antimicrobials and anticancer. Therefore, this study aimed to assess the working mechanism of active compounds obtained from the extract and the fraction of Hydroid Aglaophenia cupressina Lamoureoux against Hela ttumor cell line, as a solvent used n-hexane.  The cytotoxic activity was carried out by using MTT method.  The results showed both extract and fraction activities against Hela tumor cells. They were also categorized as moderate cytotoxic activity based on the IC50 values of the extract and fraction, namely 0.31726 µg/mL and 0.32712 µg/mL, respectively.
Biodiversity of Marine Tunicates at Samalona Waters, Sangkarang Archipelago, Indonesia Litaay, Magdalena; Santosa, Slamet; Johannes, Eva; Agus, Rosana; Moka, Willem; Darmansyah Tanjung, Jennyta Dhewi
Jurnal Ilmu Kelautan SPERMONDE VOLUME 4 NUMBER 1, 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v4i1.3616

Abstract

The study aims to know the biodiversity and community structure of marine tunicate of Samalona waters. The present study is part of biodiversity assessment for marine resources of Sangkarang Archipelago SW Makassar Indonesia. Field campaign was conducted from October to November 2016. Sample collection was done at depth of 3 m and 5 m by using Line Intersection Transect (LIT) method combination plot. Two transects size of 50 m were placed parallel to a shore line at three stations (sta.) at Samalona waters. Plot size 2.5 x 2.5 m was placed side by side of transect and all tunicate inside plot was noted, identified, counted and photographed. Sample collection was collected by using SCUBA and under water camera. Environmental parameters including water temperatue, salinity, dissolved oxygen, clarity, current speed, and wind speed were measured in situ. Data were analysed using ecological indices including species composition and density, Shanon Wienner species diversity, Evenness, and Morisita Indices. The result indicates that there are 18 species of tunicates present at depth of 3 m and 7 meters of Samalona waters, respectively. The result of the ecological analysis shows that species diversity is categorized moderate and no dominant species. Environment parameters indicatess that water quality at Samalona waters is in good condition to support tunicates.
BIODIVERSITY OF MARINE TUNICATES IN SAMALONA WATERS, SANGKARANG ARCHIPELAGO, INDONESIA Litaay, Magdalena; Santosa, Slamet; Johannes, Eva; Agus, Rosana; Moka, Willem; Tanjung, Jennyta Dhewi Darmansyah
Jurnal Ilmu Kelautan SPERMONDE VOLUME 4 NUMBER 1, 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v4i1.3920

Abstract

The study aims to know the biodiversity and community structure of marine tunicate in Samalona waters. The present study is part of biodiversity assessment for marine resources of Sangkarang Archipelago SW Makassar Indonesia. Field campaign was conducted from October to November 2016. Sample collection was done at 3 and 7 m depth by using Line Intersection Transect (LIT) method combined with a quadrat (plot). Two 50 m transects were placed parallel to a shore line at three stations (sta.) at Samalona waters. A quadrat (plot) (2.5 m x 2.5 m) was placed side by side of the line transect and all tunicates in the transect was recorded, identified, counted and photographed. Samples were collected by using SCUBA and under water camera. Environmental parameters including water temperatue, salinity, dissolved oxygen, clarity, current and wind speed, were measured in situ. Data were analysed using ecological indices including species composition and density, Shanon Wienner species diversity, Evenness, and Morisita Indices. The result indicates that there are 18 species of tunicates present at 3 m as well as 7 m depth of Samalona waters.. Result of the ecological analysis shows that species diversity can be categorized as moderate and there were no dominant species. Environmental parameters indicates that water quality at Samalona waters was in good condition to support tunicates.
Co-Authors Abdullah, As’adi Ahyar Ahmad Andi Ilham Latunra, Andi Ilham Apon Zaenal Mustopa Darmansyah Tanjung, Jennyta Dhewi Elis Tambaru Fahira, Nurul Rifqah Fahruddin Fahruddin Hanapi Usman Herwin Herwin, Herwin Magdalena Litaay Manguntungi, Baso Moka, Willem Mustika Tuwo, Mustika Rosana Agus Samawi, Muhammad Farid Sjafaraenan Sjafaraenan, Sjafaraenan Slamet Santosa St. Fauziah Syahri, Yolanda Fitria Tanjung, Jennyta Dhewi Darmansyah Vanggy, Leggina Rezzy