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All Journal Jurnal Ilmu Pertanian Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BERITA BIOLOGI JURNAL BIOLOGI INDONESIA AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Makara Journal of Science
DWINITA WIKAN UTAMI
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Journal : BERITA BIOLOGI

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IDENTIFIKASI GEN / QTL (Quantitative Trait Loci) SIFAT TOLERAN CEKAMAN ALUMINIUM PADA GALUR-GALUR PADI GOGO [Identification of Gene/QTL (Quantitative Trait Loci) for Aluminium Stress Tolerant in Upland Rice Lines] Utami, Dwinita Wikan; Rosdianti, I; Yuriyah, S; Ambarwati, AD; Hanarida, I; Suwarno, Suwarno; Miftahudin, Miftahudin
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2276

Abstract

Program on the ability of agriculture to provide rice as a staple food tends to decrease with the reduced land area used for rice cultivation. improvement rice production should be directed to use  marginal land  as dry and acid land, where poisoning aluminium was seen as a constrain factor. Development of Aluminium (Al) tolerant variety is needed to increase rice production. This technology was environmentally friendly, cheaper and also easier for farmers adopted. The rice lines with broad genetics background which tolerant to Al toxicity has been produced by breeding program. The diversity of Indonesian local rice germplasm is a basic foundation on development of Al toxicity tolerant rice varieties. The purpose of this research was to analyze the genotype variety using 384 SNPs markers on selected Al tolerant rice lines based on bioinformatics approach. Based on the 384 SNPs markers, the total 26 selected  genotypes were clustered into 5 groups. Each group was having varied Relative Root Length (RRL), which was an Al tolerant parameter of phenotype. These groups were indicated for having different genes or QTL that were related to the Al tolerant character. The association analysis obtained 9 significant SNPs markers that can be applied as the molecular markers for assisting selection in breeding  program.
SELEKSI BERBANTUKAN MARKA MOLEKULER UNTUK PEMBENTUKAN CALON VARIETAS PADI TURUNAN ESENSIAL SITU PATENGGANG Yuriyah, Siti; Utami, Dwinita Wikan; Nurani, Siti; Nasution, Anggiani; Lestari, Puji; Dadang, Ahmad; Suwarno, Suwarno
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5588.936 KB) | DOI: 10.14203/beritabiologi.v18i1.3233

Abstract

The utilization of molecular marker technology for Blast (Pyricularia grisea) resistance in rice breeding could accelerate and improve the precision of selection. This study aimed to identify and to select the BC3F2 progenies from Situ Patenggang and the opted Blast Monogenic Lines based on their resistance to blast disease in green house and field using molecular characterization. A total of 200-300 BC3F2 population strains resulted in 4 crosses between Situ Patenggang varieties and Monogenic Varieties IRBLta2-Re, IRBLkp-k60, IRBLi-F5 and IRBLa-A were used as total genetic material in the study. Blast resistance assay was initially carried out in a greenhouse and further selection was conducted in the endemic blast area, Sukabumi. The selected Molecular marker  was STS (Sequence Taq Sites) marker for foreground selection and 384-SNPs (Single Nucleotide Polymorphism) chip for background selection. The number of lines from each cross was selected by greenhouse assay i.e: 25 lines derived from Situ Patenggang/IRBLta and Situ Patenggang/IRBLkp, 21 plants for crossing Situ Patenggang/IRBLi, and 22 plants for Situ Patenggang/IRBLa-A. The results of field  experiment revealed that blast resistance response of the selected lines was varied from  0 to 5. Lines of foreground were successfully selected using STS markers specified for Pii, Pita, Pikp and Pia genes. As for the background selection by SNPs markers, some lines carried the recurrent parent genetic background, Situ Patenggang. Overall, 20 resistance lines that harbored the target genes and Situ Patenggang background were obtained. Further observation was entailed to these twenty selected lines in order to attain promising lines candidate for blast resistance.    
Co-Authors A.A. Ketut Agung Cahyawan W Ahmad Dadang Akhdya, Alina Ambarwati, AD Andari Risliawati, Andari Budi Martono Chaerani Chaerani Galurina, Rina Siti Hanarida, I Heri Syahrian, Heri Higa Afza, Higa Hilman Maulana Ida Rosdianti, Ida Indradewa, Rhian M. Khais Prayoga, M. Khais Masyuri, Agus Miftahudin . Nasution, Anggiani Nurul Hidayatun PUJI LESTARI Purwanto, Oky Dwi Rosdianti, I Shabri Shabri, Shabri Siti Nurani Siti Yuriyah Suwarno Suwarno TRI JOKO SANTOSO UTUT WIDYASTUTI Vitria Puspitasari Rahadi windarsih, gut Yuriyah, S