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All Journal Jurnal Ilmu Pertanian Indonesia Journal of Tropical Crop Science
Purwanto, Oky Dwi
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Agriculture, Biological Sciences & Forestry

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Evaluasi Mutu Fisikokimia Beras Hasil Persilangan antara Empat Padi Varietas Unggul dan Padi Liar (Oryza glaberrima dan O. rufipogon) Yuriyah, Siti; Purwanto, Oky Dwi; Afza, Higa; Dadang, Ahmad; Masyuri, Agus; Galurina, Rina Siti; Utami, Dwinita Wikan
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 3 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.3.397

Abstract

The physicochemical quality of rice is evaluated to ensure that it is fit for consumption and meets established quality standards. This study aims to analyze the physicochemical properties of rice strains resulting from crosses between four high-yielding varieties of rice and two wild rices so that the appropriate genotype is obtained for the development of quality rice. The research was conducted from November 2019 to March 2020 at the Quality Laboratory, Muara Experimental Garden, Rice Research Center, Bogor. The rice genotypes used were 22 strains resulting from crosses between high-yielding varieties of rice (Ciherang, Inpari 13, Inpari 10, and Situ Bagendit), and wild rice (Oryza glaberrima and O. rufipogon), 4 comparison varieties of crossbred elders, and two comparison varieties of organoleptic tests (Memberamo and IR42). Physical and chemical qualities were observed in terms of grain moisture content, grain density, broken skin rice yield, milled rice yield, head rice yield, rice length, rice shape, and rice liming, amylose content, gelatinization temperature, and organoleptic. The results showed that the strains 44-F-C21, 58-F-C21, and 59-F-C21 provided fairly good rice quality with head rice yields above 80% and higher than their respective elders (Situ Bagendit and Ciherang), and water content below 14%. These strains had characteristic characteristics, namely medium rice form, amylose content of 26.38%, and alkali score of 2-3. Most of these genotypes also exhibited high gelatinization temperatures, medium rice texture, and savory rice flavors. The genotype selected from the results of crosses between high-yielding varieties of rice and wild rice has the potential to be developed because there are genetic improvements from its elders on several physicochemical characteristics of rice. Keywords: amylose content; gelatinization temperature; physicochemical properties; rice lines; wild rice
Genetic Diversity and DNA Barcoding Construction of Tropical Soybean Advanced Lines Based on SSR Markers Wibisono, Kunto; Dyah, Rosliana Purwaning; Utari, Ratna; Suparjo, Suparjo; Umar, Umar; Rijzaani, Habib; Hakim, Lukman; Suhendar, Ace; Purwanto, Oky Dwi; Satyawan, Dani; Witjaksono, Witjaksono; Mastur, Mastur; Lestari, Puji; Tasma, I Made
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 2 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.2.293

Abstract

Soybean cultivation in tropical regions, such as Indonesia, is often constrained by photoperiod sensitivity, resulting in low yield. Using long juvenile traits in short photoperiod tropical areas resulted in lines with late flowering time and high yield. Genetic diversity analysis of soybean lines using molecular markers is a critical step for breeding high-yielding soybean lines. This study aimed to analyze genetic diversity and construct DNA barcodes for 44 tropical soybean superior lines based on 17 SSR markers. Genetic materials used were the high-yielding F5 soybean lines developed for their adaptation to short day-length of low latitude tropical regions. SSR markers used were those that distributed well across the soybean genome and proven their usefulness for soybean genetic diversity analyses. Results showed that the SSR demonstrated distinctive polymorphism among the 44 lines. A total of 377 alleles were detected with an average of 22.8 alleles per SSR locus. Polymorphism information content (PIC) values varied from 0.77 to 0.96 with an average of 0.90. Phylogenetic analysis showed that the 44 soybean genotypes were divided into 2 main clusters. Five markers, i.e., satt009, satt646, satt147, satt431, and satt191, with a polymorphism information content value of ≥0.94, were found to be informative and suitable for DNA barcode construction. Each of the 44 lines was assigned with specific barcodes. The barcodes constructed from this study should be useful for DNA fingerprinting as well as protection purposes of the specific superior soybean lines analyzed in this study. Keywords: DNA fingerprint, molecular markers, photoperiod, plant breeding, soybean
Growth and Yield Response of Superior Rice Varieties under Different Biofertilizer Rates in Lowland Rice Fields Purwanto, Oky Dwi; Nugraha, Dedi; Patriyawaty, Nia Romania; Wardana, I Putu
Journal of Tropical Crop Science Vol. 12 No. 03 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.03.627-638

Abstract

Efforts to increase rice productivity through a sustainable and environmentally friendly approach are using biofertilizers. The biofertilizer contains beneficial microorganisms that play an essential role in promoting plant growth and increasing rice yield. This study aimed to investigate the potential of biofertilizers and evaluate the performance of superior rice varieties by applying several rates of biofertilizers in lowland rice fields for enhancing growth and yield. A split-plot design was laid out using a randomized complete block design with three replications. The main plot consisted of superior rice varieties, including “Inpari 30”, “Inpari 32”, “Inpari 36”, and “Inpari 42”, while the subplot involved biofertilizer rates of 0, 400, and 800 g.ha-1. The utilization of biofertilizer contributed to the improvement of seedling height and root length. Applying biofertilizer increased plant height, panicle length, the number of grains per panicle, the number of filled grains per panicle, and the percentage of f illed grains, while decreasing the number of unfilled grains per panicle. The application of biofertilizer at a rate of 400 g.ha-1 significantly improved grain yields for “Inpari 30”, “Inpari 32”, and “Inpari 36”. However, “Inpari 42” required a higher biofertilizer rate of 800 g.ha-1 to produce high grain yields. The grain yield of superior rice varieties increased by 10%-21.6% with the application of biofertilizer. The findings of this study may provide recommendations for applying biofertilizers to enhance grain yields of several superior rice varieties in specific lowland rice field agroecosystems.
Co-Authors Ahmad Dadang Dani Satyawan Dedi Nugraha DWINITA WIKAN UTAMI Dyah, Rosliana Purwaning Galurina, Rina Siti HABIB RIJZAANI Higa Afza, Higa I Made Tasma I Putu Wardana, I Putu KUNTO WIBISONO Lukman Hakim Mastur Mastur Masyuri, Agus Patriyawaty, Nia Romania PUJI LESTARI Siti Yuriyah Suhendar, Ace Suparjo Umar Umar Utari, Ratna Witjaksono Witjaksono