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Uji Aktivitas Antibakteri Ekstrak Etanol Kulit Kayu Laban (Vitex pubescens Vahl) terhadap bakteri Escherichia coli Talitha Cresentia Rahma; Dyan Fitri Nugraha
Jurnal Pelayanan Kefarmasian dan Sains Vol 1 No 1 (2020): Journal Of Pharmaceutical Care and Science
Publisher : LPPM Universitas Sari Mulia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.297 KB) | DOI: 10.33859/jpcs.v1i1.36

Abstract

Background: In the world, 800.000 children die every year due to diarrheal diseases. Chronic diarrhea is usually caused by a bacterial infection, one of which is E. coli. Empirically, Dayaknese in Sampit, Central Kalimantan consuming Laban bark to treat diarrhea and dysentery. Through this research, people get scientific information about Laban wood as an alternative herbal medicine for treating diarrhea. Objective: To find out whether Laban bark can inhibit Escherichia coli bacteria and to determine the minimum inhibitory concentration in Laban bark extract that can inhibit Escherichia coli bacteria. Methods: This type of research used true experimental design with research post test only control group design. The research subjects were Escherichia coli cultured on Muller Hinton Agar (MHA) media and Laban bark extract with 3 different concentrations using the disc diffusion method. This study had 2 control groups and 3 treatment groups, DMSO as negative control, ciprofloxacin as positive control and ethanol extract of Laban bark with concentrations of 25%, 50%, and 75%. Inhibition zone diameter results were analyzed quantitatively in 3 replications. Results: The results showed that the concentrations of 25%, 50% and 75% of the extract had bacterial inhibition, in the very strong antibacterial category. Conclusion: The ethanol extract of Laban bark can inhibit the growth of Escherichia coli with an minimun inhibitory concentration 25%, which is in the very strong inhibition category. Keywords: Disc diffusion, Escherichia coli, Laban bark (Vitex pubescens Vahl)
Etnofarmakologi: Pengetahuan Khusus Yang Umum Dyan Fitri Nugraha; Kunti Nastiti; Faisal Rahman
Jurnal Pelayanan Kefarmasian dan Sains Vol 2 No 2 (2022): Journal of Pharmaceutical Care and Sciences (JPCS)
Publisher : LPPM Universitas Sari Mulia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (761.509 KB) | DOI: 10.33859/jpcs.v2i2.189

Abstract

Background: The use of traditional medicine in the community is increasing every year. Along with the development of information technology and the urgency of a pandemic, making people realize the need for traditional medicine. The development of information circulating on social media regarding the identification, efficacy, and processing of traditional medicines is not entirely correct. Objective: The high consumption and flow of information in cyberspace, especially by young people, causes the need for an analysis of the level of truth of knowledge related to traditional medicine. This is the aim of this research. Methods: Using a survey method with quantitative descriptive analysis based on online questionnaires via google form to young students (18-24 years old). The survey includes the identification, efficacy and processing of traditional medicines. Results: The average level of knowledge on identification, efficacy, and processing of traditional medicines with different levels of popularity of traditional medicines was <60%. The majority of respondents only know about traditional medicines that are commonly known, while information on the potential of other local natural resources is still not well distributed. Conclusion: The low level of respondents' knowledge of traditional medicine is a challenge for regulators, academics, and practitioners to be able to ground information about ethnopharmacology. In other words, disseminating special knowledge so that it becomes common.
Uji Aktivitas Antibakteri Ekstrak Etanol Kulit Kayu Laban (Vitex pubescens Vahl) terhadap bakteri Escherichia coli Talitha Cresentia Rahma; Dyan Fitri Nugraha
Jurnal Pelayanan Kefarmasian dan Sains Vol 1 No 1 (2020): Journal Of Pharmaceutical Care and Science
Publisher : LPPM Universitas Sari Mulia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33859/jpcs.v1i1.36

Abstract

Background: In the world, 800.000 children die every year due to diarrheal diseases. Chronic diarrhea is usually caused by a bacterial infection, one of which is E. coli. Empirically, Dayaknese in Sampit, Central Kalimantan consuming Laban bark to treat diarrhea and dysentery. Through this research, people get scientific information about Laban wood as an alternative herbal medicine for treating diarrhea. Objective: To find out whether Laban bark can inhibit Escherichia coli bacteria and to determine the minimum inhibitory concentration in Laban bark extract that can inhibit Escherichia coli bacteria. Methods: This type of research used true experimental design with research post test only control group design. The research subjects were Escherichia coli cultured on Muller Hinton Agar (MHA) media and Laban bark extract with 3 different concentrations using the disc diffusion method. This study had 2 control groups and 3 treatment groups, DMSO as negative control, ciprofloxacin as positive control and ethanol extract of Laban bark with concentrations of 25%, 50%, and 75%. Inhibition zone diameter results were analyzed quantitatively in 3 replications. Results: The results showed that the concentrations of 25%, 50% and 75% of the extract had bacterial inhibition, in the very strong antibacterial category. Conclusion: The ethanol extract of Laban bark can inhibit the growth of Escherichia coli with an minimun inhibitory concentration 25%, which is in the very strong inhibition category. Keywords: Disc diffusion, Escherichia coli, Laban bark (Vitex pubescens Vahl)