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Mitochondrial COI Haplotype Diversity of Rhynchobatus australiae Collected from Ketapang Fish Port, Bangka Belitung Islands Novita Permata Putri; Irwani Irwani; Munasik Munasik; Ranny Ramadhani Yuneni; Faqih Akbar Alghozali; Angka Mahardini
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 2 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.2.133-140

Abstract

Rhynchobatus australiae is a member of the Rhinidae family and can be found in the Indo Pacific. This species is categorized as Critically Endangered according to The International Union for Conservation of Nature and Natural Resources (IUCN) Red List and listed as Appendix II Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), due to population declines. Sustainable fisheries management is urgently needed. Information related to genetic diversity is one of the most important aspects of information for appropriate sustainable fisheries management. Therefore, this research aims to investigate the genetic diversity of Rhynchobatus australiae collected from Ketapang Fish Port Bangka Belitung Islands. Total 21 samples were collected at Ketapang Fish Port. DNA extraction was carried out using the 10% chelex method and amplification was done through PCR method on the mitochondrial DNA using Fish BCL and Fish BCH primer. Sequences in size of 653 base pairs were successfully obtained from all fish samples showing the existence of 2 clades from the construction of the phylogeny tree with close genetic distance. Results showed high haplotype diversity (Hd: 0.733 ± 0.082) and low nucleotide diversity (π: 0.00176± 0.00036) with 6 polymorphic sites (S) from 7 unique haplotypes (h). This research provides basic information of genetic diversity of Rhynchobatus australiae collected from Ketapang Fish Port and complements other information to better understand the status of the threatened Rhynchobatus australiae population.
Coral Reef Substrate Coverage in Taka Bonerate National Park Sanova, Aulia Seto Sandhi; Nugroho, Hanityo Adi; Mahardini, Angka; Agus, Elsa Lusia; Rahman, Saleh; Sutanto, Steven; Retawimbi, Aditano Yani
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8575

Abstract

Coral reef monitoring using Reef Check Method has been widely applied for conservation acts due to its handy protocols. This study aims to evaluate the coral reef conditions in the zonal areas of Taka Bonerate National Park by analyzing the substrate composition of its waters. Conducted in November 2023 using the Reef Check method, the research focused on four stations: Tinabo Besar, Tinabo Kecil, Tinanja, and Taka Lasalimu. The substrate composition was categorized into living and non-living substrates at depths of 5 and 10 meters. At 5 meters depth, the highest percentage of living substrate cover, specifically hard coral (HC), was recorded at Tinanja (74%), while the highest non-living substrate cover, dead coral/rubble (RB), was observed at Tinabo Kecil (38%). At 10 meters depth, Tinanja again showed the highest living substrate cover (HC, 40%), whereas Tinabo Kecil had the highest non-living substrate cover (RB, 41%). The findings indicate that the coral reef Substrate in the TBNP zonal area is in good condition, although some locations show damage suspected to be caused by environmentally unfriendly fishing practices. This can serve as a reference for the future management of coral reefs in TNTBR. Further research is needed to comprehensively complete the data on coral reef health, such as data on reef fish and associated invertebrates.
Short Communication: Lack of differentiation within the bigeye tuna population of Indonesia NI PUTU DIAN PERTIWI; BUDI NUGRAHA; RIRIK KARTIKA SULISTYANINGSIH; IRWAN JATMIKO; ANDRIANUS SEMBIRING; ANGKA MAHARDINI; NI KADEK DITA CAHYANI; AJI WAHYU ANGGORO; HAWIS H. MADDUPPA; PAUL H. BARBER; GUSTI NGURAH MAHARDIKA
Biodiversitas Journal of Biological Diversity Vol. 18 No. 4 (2017)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d180416

Abstract

Pertiwi NPD, Nugraha B, Kartika R, Sulistyaningsih RK, Jatmiko I, Sembiring A, Mahardini A, Cahyani NKD, Anggoro AW, Madduppa HH, Ambariyanto A, Barber PH, Mahardika GN. 2017. Short Communication: Lack of differentiation within the bigeye tuna population of Indonesia. Biodiversitas 18: 1406-1413. All highly migratory tuna and tuna-like species have vast feeding grounds and spawning grounds. Indonesia’s tuna catch is the largest in the world. However, genetic diversity in the population structure within particular tuna species in Indonesia is very limited. Here we provide genetic data for bigeye tuna (Thunnus obesus) covering fishing grounds and local fish markets throughout Indonesia. A fragment of mitochondrial DNA in the D-loop control region was amplified from samples collected across Indonesia in the biennium 2012-2013. The results showed high haplotype diversity and low nucleotide diversity in our samples. Little differentiation occured between the eleven diverse sampling locations, nor was any separation detected between general regions of Indonesia, nor between samples from fishing grounds and samples from fish markets.
PENYADARTAHUAN MASYARAKAT TERHADAP PERAN EKOLOGIS AKAR BAHAR SEBAGAI UPAYA PERLINDUNGAN EKOSISTEM TERUMBU KARANG BERBASIS MASYARAKAT DI TAMAN NASIONAL KARIMUNJAWA Aulia Seto Sandhi Sanova; Aditano Yani Retawimbi; Angka` Mahardini; Mualim Tahar; Muhammad Najwan Kamil; Muhammad Yusuf; Nenik Kholilah
Jurnal Abdi Insani Vol 13 No 7 (2026): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v13i7.3981

Abstract

The success of coral reef ecosystem conservation depends heavily on the active participation of local communities as key stakeholders. Black coral is crucial for providing habitat for numerous marine species, but is vulnerable to human exploitation due to its slow growth. Although Indonesia has legal protection for black coral, its commercialization as a souvenir continues unchecked in Karimunjawa National Park, primarily due to low public awareness. This community service activity aimed to increase understanding of black coral ecology, its vulnerability, and population status, while also fostering dialogue among community members to promote conservation adherence. The approach used included field surveys, stakeholder coordination, oral presentations, and focus group discussions (FGDs) with 15 participants from diverse backgrounds in Karimunjawa. The program's effectiveness was assessed through pre- and post-tests, demonstrating a significant increase in knowledge—from 57% correct answers initially to 92% after the intervention. Participants also expressed their hopes for addressing conservation issues in their communities. Overall, targeted education proved effective in raising awareness, laying the foundation for community-led ecosystem protection. This deeper understanding is expected to strengthen future marine conservation efforts.