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All Journal Jurnal Veteriner Microbiology Indonesia
Bibiana W Lay
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Kebuntingan Hasil Transfer Blastosis Mencit yang Dibekukan dengan Metode Vitrifikasi Kriolup I Wayan Batan; I Ketut Suatha; Ita Djuwita; Nining Handhayani; Wahono Esti Prasetyaningtyas; Ketut Adnyane Mudite; Bibiana W Lay; Supar -; Arief Boediono
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.731 KB)

Abstract

The aim of the study was to assess the viability of vitrified embryo using cryoloop as a carrier ofembryo. The blastocyst stage embryos were collected from superovulated mice. Embryos were frozenusing vitrification method and vitrified embryos were loaded on copper filament cryoloop before dipped inliquid nitrogen. The viability of vitrified embryos was assess in vitro by medium cultered and in vivo bytransfered them to recipient mice. The result shows the viability of vitrified embryos was 85,7% after 24hours cultured and the embryos were born from two pregnant recipient mice out of nine (22%) or fouroffspring out of 63 trasfered embryos (6%). In conclusion, vitrified blatocyst stage embryos using cryoloopas a carrier could keep the viability of the embryos and they could be transfered to the recipient mice andwere born normally.
Isolation and Characterization of New Antibiotics from Indonesian Coastal Marine Bacteria VERONICA VERONICA; BIBIANA W LAY; STELLA MAGDALENA
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.82 KB) | DOI: 10.5454/mi.8.3.1

Abstract

Antibiotics are organic compounds produced by various microorganisms and have the ability to inhibit the growth or kill other microorganisms. However, the irrational application of antibiotics lead to resistance of microorganisms so that they become ineffective. The objectives of this study were to isolate and characterize new antibiotics from Indonesian coastal marine bacteria. In this study, a total of 141 isolates consisting of seven Streptomyces sp. isolates and 134 isolates of other marine bacteria, were obtained from Indonesian coastal regions. Based on antimicrobial activity assay, four Streptomyces sp. and five marine bacteria isolates showed antimicrobial activity towards Bacillus cereus and Staphylococcus aureus with the diameter of inhibition of 3-12 mm. Further, antimicrobial compounds were produced successfully extracted with six organic solvents, such as 1-butanol, dichloromethane, n-hexane, chloroform, and toluene. The best solvent to extract antimicrobial compounds from Streptomyces sp. isolates was 1-butanol, while the best solvent to extract antimicrobial compounds from other marine bacteria isolates could not be specified. Antimicrobial compounds were successfully separated by thin layer chromatography with mobile phase used were 1-butanol, acetic acid, and water at a ratio of 4:1:2 and retention values obtained at 0.50 and 0.63.
Lactic Acid Bacteria from Tempeh and Their Ability to Acidify Soybeans in Tempeh Fermentation TATI BARUS; GABRIELA GIOVANIA; BIBIANA W LAY
Microbiology Indonesia Vol. 14 No. 4 (2020): December 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (974.342 KB) | DOI: 10.5454/mi.14.4.4

Abstract

Tempeh is the most famous traditional fermented food in Indonesia. Tempeh fermentation consists of two stages. In the first stage, the acidification of soybeans used bacteria around 24 hours. Lactic acid bacteria are found in tempeh. Therefore, this study is aimed to investigate the diversity of LAB from tempeh based on 16S rRNA gene sequences and to study their function in tempeh fermentation. In this study, twenty-two LAB isolates were obtained from tempeh. The isolates were closely related to Lactobacillus agilis, Lactobacillus fermentum, Weissella confusa, and Lactobacillus delbrueckii. L. fermentum (13 isolates) were the most abundant in tempeh, followed by L. agilis (7 isolates). It was found LAB important for the acidification of soybeans which the pH of soybean soaking water decreased from pH 7 to pH 4.4-4.9. Key words:  diversity, LAB, Lactobacillus,t empeh, Weissella
Co-Authors Arief Boediono GABRIELA GIOVANIA I Ketut Suatha I Wayan Batan Ita Djuwita Ketut Adnyane Mudite Nining Handhayani Stella Magdalena Supar - Tati Barus VERONICA VERONICA Wahono Esti Prasetyaningtyas