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All Journal International Journal of Biosciences and Biotechnology Cropsaver : Journal of Plant Protection
Ni Luh Putu Citra Innosensia
Tokyo University of Agriculture and Technology
Biochemistry, Genetics & Molecular Biology Environmental Science

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Identification and Characterization of Soft Rot Bacterial Pathogens on Phalaenopsis Orchid in Bali I Putu Wirya Suputra; Gusti Ngurah Alit Susanta Wirya; Nyoman Bintang Kartika Sari; I Gede Rai Maya Temaja; Ni Luh Putu Citra Innosensia
CROPSAVER Vol 5, No 1 (2022)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v5i1.39284

Abstract

The moth orchid (Phalaenopsis sp.) is one of the most popular orchids due to the various colors with distinctive shapes of the flowers. Soft rot disease caused by Pectobacteriaceae (SRP) family is commonly found infected this plant. The infected orchid showed pale-colored to blackish slimy rot. This research was conducted in three locations namely Denpasar, Badung, and Karangasem, and resulted in 10 candidates for pathogenic bacteria. The pathogenicity test of the pathogen candidates was carried out by injecting bacterial suspension into the orchid leaf tissue with the result of 6 bacterial isolates showing soft rot symptoms. Moreover, two specific primers Dda1F-Dda1R and Pcc3F-Pcc3R for Dickeya spp. and Pectobacterium spp. consecutively were used for the PCR test. The electrophoresis result of the PCR product identified the bacteria isolated from infected plants as Dickeya spp. Dickeya spp. showed white to yellowish-white colony color, with convex and circular colony form on PDA medium.
THE EFFECTIVENESS OF RHIZOBACTERIA AS BIOPROTECTANTS TO MITIGATE FUSARIUM WILT DISEASE AND AS BIOSTIMULANTS TO IMPROVE THE GROWTH OF CHILI (Capsicum annuum) Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya; I Putu Wirya Suputra; Ni Luh Putu Citra Innosensia
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p04

Abstract

The aims of this study were to examine the potential of Rhizobacteria as bioprotectants and biostimulants on chili. The potential as bioprotectants was tested by the ability of these Rhizobacteria to inhibit the growth of the pathogenic fungus Fusarium oxysporum f.sp. capsici in vitro. Moreover, as biostimulants was tested by the ability of these rhizobacteria to produce IAA compounds and their ability to increase the plant growth parameter of chili. Four rhizobacteria were challenged for antagonistic activity against F. oxysporum f.sp. capsici. Chili seeds in this experiment were soaked in four different Rhizobacteria suspension for 15 minutes. For control, seeds were soaked with sterile water instead of Rhizobacteria suspension. Results of this experiment showed that four isolates of Rhizobacteria showed strong inhibitory activity against F. oxysporum f.sp. capsici on PDA medium. Percentage of inhibitory activity varied from 77,33% to 89,79%. The application of four isolates recorded significantly increased the plant growth parameters of chili. The plant height, number of leaves, leaf area, root length, shoot dry mass, root dry mass, and chlorophyll content on treated plants significantly higher than those of untreated control plants according to the Duncan’s multiple range test (P<0.05%). These results indicate that the tested Rhizobacteria can be used as bioprotectants because they can inhibit the growth of F. oxysporum f.sp. capsici and simultaneously as a biostimulant for the ability to produce IAA compounds and stimulate the growth of chili.
Co-Authors Gusti Ngurah Alit Susanta Wirya I G. R. M. TEMAJA I PUTU WIRYA SUPUTRA Khamdan Khalimi Nyoman Bintang Kartika Sari