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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Cropsaver : Journal of Plant Protection
I PUTU WIRYA SUPUTRA
Magister Of Agricultural Biotechnology, Faculty Of Agriculture, Udayana University, Denpasar Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Identifikasi Jamur Patogen Penyebab Penyakit Layu Pucuk pada Tanaman Adenium spp. di Kota Denpasar dan Potensi Pengendaliannya dengan Jamur Antagonis DWI SUGIARTA; I PUTU SUDIARTA; NI WAYAN SUNITI; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Identification of Pathogenic Fungi that cause Wilting Diseases on Adenium spp. Shoot in Denpasar City and the Potential Control with Antagonistic Fungi Ornamental plants of Adenium, known as kamboja Jepang have various types of flowers and stem shapes and have become unique ornamental plants in Indonesia. Adenium plants that grow in Denpasar City showed the symptoms of pathogenic fungi like wilting on the shoots. This study aims to identify the pathogenic fungi that cause major diseases in Adenium spp. in the area of ??Denpasar City. The activities carried out in this study were (1) sampling, (2) isolation of the pathogenic fungi from symptomatic Adenium plant, (3) pathogenicity test, (4) morphological identification of pathogenic fungi, and (5) inhibition test of the antagonistic fungi against the pathogenic fungi. This research was conducted in Denpasar City and the Plant Disease Laboratory, Faculty of Agriculture, Udayana University. The results of this study were successful in identifying Fusarium sp. as a pathogen that causes wilting of the shoots or dieback. The potential for biological control by utilizing antagonistic fungi showed that Gliocladium sp. can suppress the growth of pathogenic fungi Fusarium sp. up to > 87% and Trichoderma sp. up to > 88 %.
New Report of Distribution of Entomopathogenic Fungi Aschersonia sp. Infected Whitefly in Bali I PUTU WIRYA SUPUTRA; I PUTU SUDIARTA; GUSTI NGURAH ALIT SUSANTA WIRYA; I KETUT SUMIARTHA
Agrotrop : Journal on Agriculture Science Vol 9 No 2 (2019)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.94 KB) | DOI: 10.24843/AJoAS.2019.v09.i02.p09

Abstract

Laporan Baru tentang Distribusi Jamur Entomopatogenik Aschersonia sp. yang Menginfeksi Kutu Putih di Bali. Penelitian tentang distribusi jamur entomopatogenik Aschersonia sp. dilakukan untuk mengetahui persebaran Aschersonia yang menginfeksi kutu putih di seluruh Bali. Penelitian tersebut dilakukan di Kabupaten Gianyar, Bangli dan Buleleng pada centra perkebunan jeruk dimulai tahun 2014 sampai tahun 2018. Aschersonia pertama kali ditemukan pada tahun 2014 di Desa Sekaan Kintamani Bangli dan Desa Kerta Payangan Kabupaten Gianyar, dan pada tahun 2018 ditemukan di Desa Gobleg Sukasada Kabupaten Buleleng. Distribusi jamur tersebut ditemukan di dataran tinggi dan tidak ditemukan di dataran rendah. Hal ini sangat terkait dengan keadaan iklim terutama kelembaban dan suhu. Jamur Aschersonia sangat baik tumbuh pada suhu 20°C yang berada pada dataran tinggi.
Identification and Characterization of Soft Rot Bacterial Pathogens on Phalaenopsis Orchid in Bali I Putu Wirya Suputra; Gusti Ngurah Alit Susanta Wirya; Nyoman Bintang Kartika Sari; I Gede Rai Maya Temaja; Ni Luh Putu Citra Innosensia
CROPSAVER Vol 5, No 1 (2022)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v5i1.39284

Abstract

The moth orchid (Phalaenopsis sp.) is one of the most popular orchids due to the various colors with distinctive shapes of the flowers. Soft rot disease caused by Pectobacteriaceae (SRP) family is commonly found infected this plant. The infected orchid showed pale-colored to blackish slimy rot. This research was conducted in three locations namely Denpasar, Badung, and Karangasem, and resulted in 10 candidates for pathogenic bacteria. The pathogenicity test of the pathogen candidates was carried out by injecting bacterial suspension into the orchid leaf tissue with the result of 6 bacterial isolates showing soft rot symptoms. Moreover, two specific primers Dda1F-Dda1R and Pcc3F-Pcc3R for Dickeya spp. and Pectobacterium spp. consecutively were used for the PCR test. The electrophoresis result of the PCR product identified the bacteria isolated from infected plants as Dickeya spp. Dickeya spp. showed white to yellowish-white colony color, with convex and circular colony form on PDA medium.
THE EFFECTIVENESS OF RHIZOBACTERIA AS BIOPROTECTANTS TO MITIGATE FUSARIUM WILT DISEASE AND AS BIOSTIMULANTS TO IMPROVE THE GROWTH OF CHILI (Capsicum annuum) Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya; I Putu Wirya Suputra; Ni Luh Putu Citra Innosensia
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p04

Abstract

The aims of this study were to examine the potential of Rhizobacteria as bioprotectants and biostimulants on chili. The potential as bioprotectants was tested by the ability of these Rhizobacteria to inhibit the growth of the pathogenic fungus Fusarium oxysporum f.sp. capsici in vitro. Moreover, as biostimulants was tested by the ability of these rhizobacteria to produce IAA compounds and their ability to increase the plant growth parameter of chili. Four rhizobacteria were challenged for antagonistic activity against F. oxysporum f.sp. capsici. Chili seeds in this experiment were soaked in four different Rhizobacteria suspension for 15 minutes. For control, seeds were soaked with sterile water instead of Rhizobacteria suspension. Results of this experiment showed that four isolates of Rhizobacteria showed strong inhibitory activity against F. oxysporum f.sp. capsici on PDA medium. Percentage of inhibitory activity varied from 77,33% to 89,79%. The application of four isolates recorded significantly increased the plant growth parameters of chili. The plant height, number of leaves, leaf area, root length, shoot dry mass, root dry mass, and chlorophyll content on treated plants significantly higher than those of untreated control plants according to the Duncan’s multiple range test (P<0.05%). These results indicate that the tested Rhizobacteria can be used as bioprotectants because they can inhibit the growth of F. oxysporum f.sp. capsici and simultaneously as a biostimulant for the ability to produce IAA compounds and stimulate the growth of chili.
Identifikasi Penyebab Penyakit Bercak Merah pada Tanaman Stroberi (Fragaria sp.) di Bali dan Potensi Pengendaliannya dengan Jamur Antagonis Secara In Vitro I GUSTI AYU ARI SANTIKADEWI; GUSTI NGURAH ALIT SUSANTA WIRYA; TRISNA AGUNG PHABIOLA; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Identification of Pathogenic Fungi causes Red Leaf Spot on Strawberry Plants (Fragaria sp.) in Bali and Control Potential with Antagonist Fungi Strawberry (Fragaria sp.) is a subtropical fruit plant that was first discovered in Chile, America and has high economic value. Strawberry plants growing in Bali show symptoms of pathogenic fungi attack. Symptoms include red spots on the leaf surface. The purpose of this study was to identify pathogenic fungi that cause disease in strawberry plants in the center of strawberry cultivation in Bali. The activities carried out in this study were (1) sampling, (2) isolation of pathogenic fungi from symptomatic strawberry plant parts, (3) pathogenicity test, (4) morphological identification of pathogenic fungi, and (5) potential inhibition test of antagonistic fungi. against pathogenic fungi. This research was conducted in Pancasari Village, Buleleng Regency and Candi Kuning and Kembang Mertha Villages, Tabanan Regency and continued at the Plant Disease Laboratory, Faculty of Agriculture, Udayana University, Denpasar. The results of this study succeeded in identifying Pestalotiopsis sp. as a pathogen causingred spot disease on strawberry leaves in Bali and the potential for biological control using antagonistic fungi in vitro showed that Gliocladium sp. and Trichoderma asperellum can suppress the growth of pathogenic fungi on strawberry plants by > 80%, Trichoderma viride up to > 90% and Trichoderma koningii and Trichoderma harzianum up to > 50%.
Identifikasi Penyebab Penyakit Bercak Daun Mycosphaerella pada Tanaman Stroberi (Fragaria sp.) di Bali dan Potensi Pengendaliannya dengan Jamur Antagonis secara In Vitro GUSTI AYU DWITA ANDRAWINA; GUSTI NGURAH ALIT SUSANTA WIRYA; NI WAYAN SUNITI; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Identification of Pathogenic Fungi causes Mycosphaerella Leaf Spot on Strawberry Plants (Fragaria sp.) in Bali and Control Potential with Antagonist Fungi Strawberry (Fragaria sp.) is a fruit cultivation plant that develops in Indonesia and has attractiveness and high economic value. Strawberry plants that grow at the center of strawberry production in Bali show symptoms of pathogenic fungus attack. Symptoms caused by the presence of brownish purple spots with a white center on the leaves. The purpose of this study was to identify pathogenic fungi that caused Mycosphaerella leaf spot disease on strawberry plants located at the center of strawberry production in Bali. The activities carried out in this study were (1) sampling and calculating the percentage of Mycosphaerella leaf spot disease on strawberry plants, (2) isolation of pathogenic fungi from symptomatic leaves of strawberry plants, (3) pathogenicity testing, (4) identification of pathogenic fungi, and (5) test the potential inhibition of antagonistic fungi against pathogenic fungi. This research was conducted in Candi Kuning and Br Kembang Merta Village, Tabanan Regency and Pancasari Village, Buleleng Regency, and continued at the Plant Disease Laboratory, Faculty of Agriculture, Udayana University. The results of this study were successful in identifying Mycosphaerella fragariae as a pathogen causing leaf spot disease on strawberry plants in Bali and the potential for biological control by utilizing antagonistic fungi in vitro showed that Trichoderma viride, Trichoderma asperellum, Gliocladium sp. can suppress the growth of pathogenic fungi in strawberry plants of > 90%, Trichoderma harzianum and Trichoderma koningii up to >80%.
Metode Isolasi Jamur Patogen Serangga (Aschersonia placenta) Menggunakan Media Water Agar dan Potato Sucrose Agar NI MADE SAVITA RASJMAN; I PUTU SUDIARTA; TRISNA AGUNG PHABIOLA; GUSTI NGURAH ALIT SUSANTA WIRYA; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Isolation Method of Insect Pathogenic Fungi (Aschersonia placenta) using Water Agar Media and Potato Sucrose Agar Bali Province is one of the provinces that is famous for its superior agricultural commodities. One of the leading agricultural commodities is citrus. The production of citrus fruits in Bali Province in 2020 is 4,903,341 quintals based on data from the Central Statistics Agency for Bali Province 2021. However, in some citrus-producing areas, citrus fruit production has decreased compared to the previous year. The decline in citrus fruit production is caused by several factors, one of which is the attack of whitefly pests. Whitefly pests can be controlled by utilizing the insect pathogenic fungus Aschersonia placenta. This study aims to determine the isolation method of insect pathogenic fungi Aschersonia placenta using water agar media and potato sucrose agar. The study began with sampling, isolated the fungus and identifying the fungus by morphological characteristics. The results of this study showed that isolation method of A. placenta using water agar media and potato sucrose agar effective for multiply the fungus.
Co-Authors DWI SUGIARTA GUSTI AYU DWITA ANDRAWINA Gusti Ngurah Alit Susanta Wirya I G. R. M. TEMAJA I GUSTI AYU ARI SANTIKADEWI I KETUT SUMIARTHA I Putu Sudiarta Khamdan Khalimi Ni Luh Putu Citra Innosensia NI MADE SAVITA RASJMAN NI WAYAN SUNITI Nyoman Bintang Kartika Sari TRISNA AGUNG PHABIOLA