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Rachmad Bayu Kuncara
Poltekkes Kemenkes Semarang
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The Effect Of Various Sun Time Under Exposure To Ultraviolet Light Of The Sun On Interleukin 10 Cytokine Rachmad Bayu Kuncara
Jurnal Biosains Pascasarjana Vol. 24 No. 1 (2022): VOL 24, NO 1 (2022): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1.2022.29-33

Abstract

Sunbathing activities are often found to occur during the severe acute respiratory syndrome coronavirus 2 (SARS CoV2) pandemic that causes Corona Virus Disease 2019 (COVID 19). IL-10 is an inhibitory cytokine for macrophages and dendritic cells that play a role in controlling non-specific and cellular immune reactions. The purpose of this study was to explain the immunosuppression by the cytokine interleukin 10 which is often found to occur due to sun exposure. This research method is an experimental study with a completely randomized design (CRD). The research design used was a randomized post test only control group design. Data were analyzed using the Kruskal Wallis test with statistical test results obtained p value 0.550 (> 0.05) meaning there was no difference in the levels of cytokine interleukin 10 in each treatment group.
THE EFFECT OF CHOLECALCIFEROL (VITAMIN D3) LEVELS ON VARIOUS TIMES AND DURATIONS OF EXPOSURE TO ULTRAVIOLET LIGHT (SUN EXSPOSURES) Rachmad Bayu Kuncara; Roni Afriansya
Jurnal Biosains Pascasarjana Vol. 25 No. 2 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i2.2023.117-122

Abstract

Sunbathing triggers the vitamin D production, which functions to increase the immune system. Vitamin D is very beneficial for the health of the body, exposure to sunlight in sufficient time the body will get adequate vitamin D. The purpose of this study is to explain the level of vitamin D3 levels that are often found to occur due to exposure to ultraviolet rays of the sun (Sun Exposures). This research method is an experimental study with a completely randomized design (CRD). The research design used was a randomized post test only control group design. Data were analyzed using the Kruskal Wallis test with statistical test results obtained levels of Cholecalciferol (Vitamin D3) with p value = 0.675 (> 0.05) meaning there was no difference in each treatment group.
Perbedaan Kadar Vitamin D3 pada Penderita Tuberkulosis Paru Sebelum dan Sesudah Satu Bulan Terapi Obat Anti Tuberkulosis Rachmad Bayu Kuncara; Dewina Zumrotin Najiah
Jaringan Laboratorium Medis Vol 6 No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.11379

Abstract

Pulmonary tuberculosis is an infectious disease caused by infection with the bacteria Mycobacterium tuberculosis. Patients diagnosed with pulmonary tuberculosis will receive Anti-Tuberculosis Drug (OAT) therapy for six months. In the first two months or what is called the intensive phase, the patient will be given a combination of OAT in the form of rifampicin, isoniazid, ethambutol and pyrazinamide. Several types of anti-tuberculosis drugs have the potential to affect vitamin D3 metabolism, either by interfering with absorption, metabolism or changing the action of vitamin D3. The long-term treatment process for pulmonary tuberculosis also results in a decrease in vitamin D3 levels in the blood. This study aims to determine the difference in vitamin D3 levels in pulmonary tuberculosis sufferers before and after one month of anti-tuberculosis drug therapy. This type of research is descriptive observational with a prospective cohort design without comparison. The total sample was 16 newly diagnosed pulmonary tuberculosis sufferers at the Bangetayu, Kedungmundu and Tlogosari Wetan Health Centers, Semarang City. Vitamin D3 levels in serum samples were examined using the ELISA method. The mean vitamin D3 level before OAT therapy was 19.00 ± 10.45 ng/mL, the level decreased after OAT therapy, namely 18.51 ± 4.74 ng/mL. These results indicate that administration of anti-tuberculosis drugs may have an effect on vitamin levels.
Derajat Aglutinasi pada Pemeriksaan Golongan Darah Metode Tabung Berdasarkan Masa Simpan Test Sel A dan Test Sel B Hari Ke-0, Ke-2, Ke-4, Ke-6 dan Ke-8 Karinta Syifa Zatalini; Rachmad Bayu Kuncara; Anung Sugihantono
Jaringan Laboratorium Medis Vol 6 No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.11733

Abstract

Blood group examination is an examination that aims to determine the type of blood group. Cell test is a blood group examination reagent used to detect antibodies in the serum being examined. The long shelf life of cell tests that can only last for two days is considered less effective for agencies with a high level of blood services. The purpose of this study was to describe the degree of agglutination in blood group examination with cell test A and B stored on the 0th, 2nd, 4th, 6th, and 8th days. This study is a descriptive study with a Quasi Experimental research design. Test cell A and test cell B were made from red blood cell specimens of 3 blood type A and 3 blood type B respectively. Test cells are stored in a refrigerator with a temperature of 2-6° C. Test cells were then examined on the 0th, 2nd, 4th, and 6th day of storage. Calculation of samples and repetitions using the Federer formula with the number of treatments in this study is 5 treatments. Based on the calculation, one sample of test cell A and test cell B was obtained with five repetitions of each examination. The results showed that on the 0th, 2nd, 4th, and 6th day of cell test storage, the results of blood type examination were obtained, namely the degree of agglutination 4+ with erythrocytes in the cell test clumping into one bond, cells forming large agglutination with clear supernatant. On the 8th day of storage, the result of agglutination degree is 3+ with erythrocytes in test cells not clumping perfectly, there are erythrocyte granules and cloudy supernatant. Based on the results of the study, it is concluded that test cell A and test cell B can be used optimally until day 6 storage.
Perbedaan Kadar TNF-α pada Penderita Tuberkulosis Paru Sebelum dan Sesudah Satu Bulan Terapi Obat Anti Tuberkulosis Rachmad Bayu Kuncara; Devi Etivia Purlinda; Yoki Setyaji; Wiwit Sulistyasmi
Jaringan Laboratorium Medis Vol 7 No 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12789

Abstract

Pulmonary tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Patients diagnosed with pulmonary tuberculosis undergo Anti-Tuberculosis Drug (OAT) therapy for six months. In the first two months, known as the intensive phase, patients receive a combination of OATs such as isoniazid, rifampicin, ethambutol, and pyrazinamide. TNF-α is a pro-inflammatory cytokine that plays a crucial role in granuloma formation to contain the spread of Mycobacterium tuberculosis. This study aims to determine the difference in TNF-α levels in pulmonary tuberculosis patients before and after one month of OAT therapy. The study used an observational analytic design with a prospective cohort approach without comparison. A total of 17 newly diagnosed pulmonary tuberculosis patients at Bangetayu, Kedungmundu, and Tlogosari Wetan Health Centers, Semarang City, were included. TNF-α levels in serum samples were measured using the ELISA method. The mean TNF-α level before OAT therapy was 110.09±100,48 pg/mL, while after one month of therapy, it decreased to 90,05±60,12 pg/mL. The statistical analysis using the Mann-Whitney test yielded a p-value of 0.228 (p > 0.05), indicating that there was no statistically significant difference in TNF-α levels before and after one month of anti-tuberculosis drug (OAT) therapy. However, the mean TNF-α levels showed a decreasing trend, which, although not statistically significant, may suggest an initial immunological response to the treatment. The researcher recommends further studies with a larger sample size and longer observation period—such as until the end of the intensive phase or the completion of therapy—in order to obtain more representative and statistically significant results regarding TNF-α dynamics during tuberculosis treatment.
Co-Authors Anung Sugihantono Devi Etivia Purlinda Dewina Zumrotin Najiah Karinta Syifa Zatalini Roni Afriansya Wiwit Sulistyasmi Yoki Setyaji