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Agama dan Pro-Kontra Gerakan Dakwah Radikal di Indonesia Miftakun Niam; Muhammad Bimo Ginantoko; Sahrul Ramadhani; Wahyu Pratiwi; Tafsir Tafsir; Ismail Ismail; Ahmad Fauzan Hidayatullah
Jurnal Ilmu Agama: Mengkaji Doktrin, Pemikiran, dan Fenomena Agama Vol 23 No 1 (2022): Jurnal Ilmu Agama : Mengkaji Doktrin, Pemikiran, dan Fenomena Agama
Publisher : Fakultas Ushuluddin dan Pemikiran Islam Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/jia.v23i1.13025

Abstract

Radicalism does not only revolve around religious ideology, but has also been incarnated in social, political and cultural life. That is, any ideology or thought that has a negative impact (effect) causes a person to become militant and fanaticism can be included in radicalism. Religious radicalism has recently emerged, inviting someone to adapt to other people. This concerns the practice of life (mu'amalah) and worship (ubudiyah), especially regarding the different views on their religion. Different interpretations see that religious law is exacerbated by selfish reasons that lead to the breakdown of harmony in society. What is considered not in accordance with the same understanding, is considered to deviate from the true teachings of Islam. Later, many influential people asked the person to return to the true religious teachings. He views hat religious teachings must deviate from the practice of life. Unfortunately, this true teaching is only based on their own understanding. For him, the teachings he received were considered pure and represented the true and legitimate teachings of Islam. If things like this continue, then surely the internal division of religious communities will be wide open and it turns out that the mass organization movement that smells of radicalism in the name of religion does not only happen to Islam, but also to others. religion.
PERBANDINGAN PROFIL TOTAL DNA DURIAN (Durio zibethinus Murr) VARIETAS MENOREH KUNING HASIL ISOLASI DENGAN METODE DETERGEN DAN KIT KOMERSIAL Tara Puri Ducha Rahmani; Budi Setiadi Daryono; Ismail Ismail
Teknosains Vol 16 No 2 (2022): Mei-Agustus
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v16i2.29385

Abstract

Menoreh Kuning Durian (Durio zibethinus Murr) cultivar is originated from Dusun Promasan, Banjaroyo Village, Kalibawang Subdistrict, Kulonprogo Regency, Province of Yogyakarta, Indonesia. Durian seller usually claimed their durian to be Menoreh Kuning. Therefore, an accurate method is needed to identify that cultivar. One of the most accurate methods is DNA isolation as the first step. This research was aimed to compare the total DNA profile of Menoreh Kuning Durian using detergent and commercial kit (NucleonTM PhytopureTM Genomic DNA Extraction Kits) qualitatively and quantitatively. The method used in this research was DNA isolation method using detergent and commercial kit (NucleonTM PhytopureTM Genomic DNA Extraction Kits). DNA source came from leaves Menoreh Kuning Durian. DNA pellet then was dissolved in 50 µL TE, after that each of 5 µL sampel was electrophoresized in 1 % of agarose for 30 minutes. The result of eletcrophoresis then was stained with ethidium bromide and finally was observed under transluminator-UV. The results of both isolation methods were compared based on the total DNA profil after electrophoresis, total mass of DNA pellet, and the result of DNA Optical Density. The research result had shown that the DNA of Menoreh Kuning Durian isolated using detergent and commercial kit can be observed under transluminator-UV. The mass of DNA pellet isolated using detergent was 0,004 gram while using kit was 0,0004 gram. The Optical Density results for DNA isolated using detergent were 2,658 AU of Absorbance, Ratio of 1,057, Concentration of 6645,6 µ gr/mL, and Protein of 93,9 milligram/mL. As for the DNA isolated using commercial kit had shown the Optical Density results of 2,760 AU for Absorbance, Ratio of 1,339, concentration of 6900,6 µ gr/mL, and Protein of 54,8 milligram/mL. Based on the research result, can be concluded that the DNA of Menoreh Kuning Durian can be isolated using detergent and commercial kit. The DNA profile isolated using commercial kit had shown a better result than the DNA profile isolated using detergent.