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All Journal Biogenesis: Jurnal Ilmiah Biologi Teknosains: Media Informasi Sains dan Teknologi Prosiding Seminar Biologi Journal of Natural Sciences and Mathematics Research Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi AL-HAYAT: Journal of Biology and Applied Biology Berkala Ilmiah Biologi
Tara Puri Ducha Rahmani
UNIVERSITAS ISLAM NEGERI WALISONGO SEMARANG
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Veterinary

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Menilik potensi biologi sebagai peluang usaha di masa pandemi Riski Apriliyani; Septi Lailia Suknia; Tara Puri Ducha Rahmani
Prosiding Seminar Biologi Vol 7 No 1 (2021): PROSIDING BIOLOGI ACHIEVING THE SUSTAINABLE DEVELOPMENT GOALS WITH BIODIVERSITY I
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v7i1.24913

Abstract

Dampak kebijakan pemerintah bagi masyarakat mengenai adanya pandemi COVID-19 salah satunya adalah pemutusan hubungan kerja. Tujuan dilakukan penelitian ini yaitu untuk mengetahui potensi biologi sebagai peluang usaha di masa pandemi dan jenis usaha yang dapat dilakukan sesuai dengan ilmu biologi. Metode dalam penelitian ini adalah metode deskripsi yang menjabarkan setiap potensi usaha yang dapat dilakukan sesuai dengan keilmuan biologi.  Usaha bidang biologi atau disebut dengan bioentrepreneurship menjadi jawaban dari permasalahan ekonomi ini, beberapa usaha yang dapat dilakukan dan menguntungkan diantaranya, usaha budikdamber (budidaya ikan dalam ember) berbasis aquaponik dimana usaha ini mencakup dua aspek yaitu budidaya ikan dan sayuran, usaha tanaman hias dapat dijadikan salah satu usaha yang termasuk dalam hortikultura tanaman hias atau disebut florikultur, usaha obat-obatan herbal untuk meningkatkan imun, usaha makanan berbasis biologi yang mudah untuk dilakukan, serta usaha pembuatan pupuk kompos dari limbah rumah tangga. Pembuatan kompos ini selain bermanfaat untuk tanaman sekaligus mengurangi sampah di lingkungan tempat tinggal.
Kajian budidaya tanaman anggrek Dendrobium sp. menggunakan teknik kultur meristem serta pengaruh penambahan berbagai ekstrak terhadap pertumbuhannya Syifara Chika; Feby Kurniawati; Tara Puri Ducha Rahmani
Prosiding Seminar Biologi Vol 7 No 1 (2021): PROSIDING BIOLOGI ACHIEVING THE SUSTAINABLE DEVELOPMENT GOALS WITH BIODIVERSITY I
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v7i1.24915

Abstract

Produksi budidaya anggrek Dendrobium sp di Indonesia relatif lambat, sedangkan peminatnya selalu meningkat pada setiap tahunnya. Perbanyakan tanaman anggrek dengan cara konvensional memerlukan waktu yang cukup lama untuk memperoleh hasil anakan tanaman dalam jumlah yang banyak, oleh karena itu dibutuhkan alternatif budidaya menggunakan teknik kultur jaringan. Kultur jaringan merupakan teknik perbanyakan sel, jaringan atau organ tanaman dengan ditumbuhkan pada medium buatan (in vitro) secara aseptik. Salah satu teknik dalam kultur jaringan tanaman adalah kultur meristem. Kultur meristem ini menggunakan eksplan yang terdapat pada jaringan meristem. Pada teknik kultur meristem dapat menggunakan meristem tunas aksilar atau meristem pada pucuk terminal. Pemilihan ekstrak suplemen pertumbuhan yang tepat sangat berpengaruh terhadap pertumbuhan planlet. Tujuan penelitian ini adalah untuk mengkaji mengenai pengaruh penambahan ekstrak ragi, ekstrak jagung (Zea mays L.), ekstrak air kelapa (Cocos nucifera L.), ekstrak tomat (Solanum lycopersicum L.) pada pertumbuhan kultur anggrek Dendrobium sp. Metode yang dilakukan yaitu studi literatur dan pengumpulan data dari berbagai sumber artikel terdahulu. Dari hasil studi literatur, didapatkan bahwa pemberian ekstrak ragi yang mengandung nutrisi dapat memberikan pengaruh pada jumlah daun dan tinggi planlet. Pada penambahan ekstrak jagung terdapat pengaruh pada jumlah daun, tinggi planlet dan jumlah tunas. Pemberian air kelapa memberi pengaruh yang efektif pada pertumbuhan jumlah tunas planlet, tetapi tidak mempengaruhi banyaknya jumlah daun dan tinggi planlet. Sedangkan pada penambahan ekstrak tomat memberikan hasil terbaik untuk meningkatkan tinggi planlet, jumlah daun, dan pertumbuhan tunas. Pemilihan bahan organik untuk regenerasi tanaman anggrek Dendrobium sp dipilih berdasarkan jenis tanaman anggrek Dendrobium sp yang akan ditanam dengan tujuan untuk mendapatkan jumlah tunas planlet yang diinginkan. Hal tersebut disebabkan karena penggunaan bahan organik tertentu memberikan hasil yang spesifik dan bisa berbeda hasilnya saat diaplikasikan pada jenis anggrek Dendrobium sp lainnya.
Parasitic Protozoa found in the skin, gills, and intestines of Patin Catfish (Pangasius hypophthalmus) and Common Carp (Cyprinus carpio) Veenu Kumar; Arifin Budiman Nugraha; Umi Cahyaningsih; Tara Puri Ducha Rahmani
Journal of Natural Sciences and Mathematics Research Vol 7, No 2 (2021): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.2021.7.2.11493

Abstract

The parasitic protozoa found in "Ikan Patin" or a species of Catfish (Pangasius hypophthalmus) and "Ikan Mas" or Common Carp (Cyprinus carpio) are Trichodina sp., Ichthyophthirius sp. and Myxobolus sp. These protozoa have the potential to cause death in fish and economic losses to fish farmers and sellers. This study was conducted to determine the presence of parasitic protozoa on the skin, gills, and intestines of Catfish and Carp. Samples were taken from the Bursa Ikan Hias Laladon or Laladon Ornamental Fish Market in Bogor, Indonesia. Each species was sampled for as many as 30 fish. The skin, gills, and intestines were examined using the native examination method and Lugol staining. Parasitic protozoa were identified based on their morphology, namely size, and shape. The types of parasitic protozoa found on the skin, gills, and intestines of Catfish and goldfish were Trichodina sp., Ichthyophthirius sp., and Myxobolus sp. The parasites were found in skin organs in as many as 29 samples (96.7%) from Catfish and 30 samples (100%) from Carp. The Genera of Ichthyophthirius sp. were found in the gills of 2 samples (6.7%) in Catfish and 1 sample (3.3%) from positive Carp. Myxobolus sp. was found in the intestines of 11 samples (36.7%) from Catfish and two samples (6.7%) from Carp. The species of Trichodina sp. found based on morphology was Trichodina giurusi.©2021 JNSMR UIN Walisongo. All rights reserved.
Simple Feasibility Analysis Of Nitrogen-Fixing Cereals Project Tara Puri Ducha Rahmani; Dian Aruni Kumalawati; Dian Ayuning Tyas; Dian Triastari Armanda; Rusmadi Rusmadi
Al-Hayat: Journal of Biology and Applied Biology Vol 3, No 2 (2020)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v3i2.6082

Abstract

Nitrogen does not directly have advantages in human physiology system, but it holds one of the most critical roles in plants’ life cycle and productivity. Even though Nitrogen is the most abundant elements in the atmosphere, it is also the most deficient essential nutrients in plants. The proposed idea of the nitrogen-fixing GM crops, particularly wheat, is aimed to overcome those stated cons of the traditional diculture and nitrogen fertilizer. This analysis focus on the overview as well as the pro and cons of the genetically modified nitrogen-fixing plants in providing a better agricultural method. The genetically modifying method to generate a nitrogen-fixing non-legumes carries a significant chance of failure results and hindrance. The multilevel implication occurs when we need to modify the plants that not normally produce nodules in their roots to form the nodules and to modify the Nitrogen-fixing microbes to live in the nodules of non-legumes, which are not their natural dwelling places.In conclusion, the genetically modified crops project to fix their Nitrogen is feasible, but the difficulties and the funds needed still outweigh the benefits obtained in the future. With all of those limitations, the target goal to erase famine in 2050 just by funding the nitrogen-fixing wheat alone seems to be too high to be reached. The funds and efforts should be better spent on other factors and farming methods.
The potency of bacteria isolated from the hydroponic rockwool of field mustard (Brassica rapa L.) for nitrogen fixation and indole acetic acid (IAA) production Anindita Riesti Retno Arimurti; Vella Rohmayani; Nurhidayatullah Romadhon; Tara Puri Ducha Rahmani; Lee James Watson; Kenari Sri Wahyuni; Nova Ulumiya
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 1 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i1.28451

Abstract

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PERBANDINGAN PROFIL TOTAL DNA DURIAN (Durio zibethinus Murr) VARIETAS MENOREH KUNING HASIL ISOLASI DENGAN METODE DETERGEN DAN KIT KOMERSIAL Tara Puri Ducha Rahmani; Budi Setiadi Daryono; Ismail Ismail
Teknosains Vol 16 No 2 (2022): Mei-Agustus
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v16i2.29385

Abstract

Menoreh Kuning Durian (Durio zibethinus Murr) cultivar is originated from Dusun Promasan, Banjaroyo Village, Kalibawang Subdistrict, Kulonprogo Regency, Province of Yogyakarta, Indonesia. Durian seller usually claimed their durian to be Menoreh Kuning. Therefore, an accurate method is needed to identify that cultivar. One of the most accurate methods is DNA isolation as the first step. This research was aimed to compare the total DNA profile of Menoreh Kuning Durian using detergent and commercial kit (NucleonTM PhytopureTM Genomic DNA Extraction Kits) qualitatively and quantitatively. The method used in this research was DNA isolation method using detergent and commercial kit (NucleonTM PhytopureTM Genomic DNA Extraction Kits). DNA source came from leaves Menoreh Kuning Durian. DNA pellet then was dissolved in 50 µL TE, after that each of 5 µL sampel was electrophoresized in 1 % of agarose for 30 minutes. The result of eletcrophoresis then was stained with ethidium bromide and finally was observed under transluminator-UV. The results of both isolation methods were compared based on the total DNA profil after electrophoresis, total mass of DNA pellet, and the result of DNA Optical Density. The research result had shown that the DNA of Menoreh Kuning Durian isolated using detergent and commercial kit can be observed under transluminator-UV. The mass of DNA pellet isolated using detergent was 0,004 gram while using kit was 0,0004 gram. The Optical Density results for DNA isolated using detergent were 2,658 AU of Absorbance, Ratio of 1,057, Concentration of 6645,6 µ gr/mL, and Protein of 93,9 milligram/mL. As for the DNA isolated using commercial kit had shown the Optical Density results of 2,760 AU for Absorbance, Ratio of 1,339, concentration of 6900,6 µ gr/mL, and Protein of 54,8 milligram/mL. Based on the research result, can be concluded that the DNA of Menoreh Kuning Durian can be isolated using detergent and commercial kit. The DNA profile isolated using commercial kit had shown a better result than the DNA profile isolated using detergent.
Potential of biological agents (Pseudomonas sp.) in plastic waste biodegradation process Aisyah Chofifawati; Robbi Mauizzatul Hikmah; Nilana Izzati; Lathifah Nurul Fauzi; Tara Puri Ducha Rahmani; Arnia Sari Mukaromah
Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 5, No 2 (2022): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v5i2.1192

Abstract

Pseudomonas sp. can degrade plastics because it has an inducible operon system that produces certain enzymes (esterase, serine, hydrolase, and lipase) in the process of carbon source metabolism. The purpose of this study was to determine the potential of Pseudomonas sp. as a plastic biodegradation agent. This research method is a systematic literature review which is carried out by collecting, understanding, analyzing and concluding as many as 30 national and international journal articles published from 2008 to 2022 from several search engines such as Google Scholar, Ncbi, Sinta, and Plos one with the keywords degrading microorganisms plastic. The steps of this research were made samples to be used, tested samples with bacterial isolation, filtered the bacteria, bacterial bacteria and biodegradation within a specified time period by looking at the % loss of plastic before treatment. The results of the literature review analysis showed that Pseudomonas sp. able to degrade plastic around 2-19% by utilizing plastic as a carbon source which will convert addition and condensation polymers into simple molecules such as monomers, dimers and oligomers during the inventory period within 1-3 months. Thus, the microorganisms in the form of Pseudomonas sp. can decompose plastic waste naturally and faster than other methods and does not cause adverse effects.
The role of indigenous lactic acid Bacteria Genus Lactobacillus in the fermentation process of Durian (Durio zibethinus) for Tempoyak production Yunita Ayu Ardilla; Krisna Wahyu Anggreini; Tara Puri Ducha Rahmani
Berkala Ilmiah Biologi Vol 13 No 2 (2022): Jurnal Berkala Ilmiah Biologi
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v13i2.4619

Abstract

Durian (Durio zibethinus) is a type of fruit included in the climacteric fruit, so it needs processing to have a longer shelf life. One of the processed durian fruits is tempoyak. Tempoyak is a traditional fermented durian food well known around Kalimantan and Sumatra. Tempoyak is made from durian, which usually has a good level of maturity. The distinctive aroma and semi-solid texture of tempoyak are produced by the fermentation process by bacteria. Tempoyak processing has involved adding a bit of salt and indigenous lactic acid bacteria, one of which is the bacteria genus Lactobacillus, such as Lactobacillus plantarum and Lactobacillus curvatus. This study aimed to determine the role of lactic acid bacteria from the genus Lactobacillus in the fermentation process of durian into tempoyak, to determine the potential of bacteria from the genus Lactobacillus in other respects, and to determine the health benefits of tempoyak. This research was conducted using a literature study method with several stages, namely article searching, article selection, data synthesis, and ending with report generation. From the literature search, it can be seen that indigenous bacteria from the genus Lactobacillus are found in fermented durian fruit. Lactobacillus plantarum and Lactobacillus curvatus are species of the genus Lactobacillus that can ferment sugar into lactic acid and are usually used in fermented agricultural products and livestock products. Lactobacillus plantarum producing lactic acid is amylolytic because it can directly convert starch to form lactic acid. Lactobacillus curvatus has various functions related to carbohydrate utilization and bacteriocin production, enabling fermentative and antibacterial properties. Tempoyak can be beneficial for digestive health and has the potential to increase the body's immune system. The results obtained prove that the indigenous bacteria of the genus Lactobacillus from tempoyak play a role in the durian fermentation process and have the potential as probiotics that are good for health.
Isolation and characterization of Bacteria in the ancient Javanese Pegon manuscript: “Layang Watu Gunung” Tara Puri Ducha Rahmani; Nur Ahmad; Anasom Anasom; Salsabiela Pertiwi; Muhammad Yusrun Niam; Hamdan Hadi Kusuma; Lee James Watson
Journal of Natural Sciences and Mathematics Research Vol. 9 No. 2 (2023): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.v9i2.22679

Abstract

This research is one of the efforts and the first step in the preservation of historical ancient manuscripts with an interdisciplinary scientific approach. The Unity of Sciences involved in this research are historical science, archaeology, philology combined with natural sciences such as microbiology and biotechnology approaches. The ancient manuscript whose microorganisms were isolated and observed in this study is an ancient manuscript with Pegon letters titled "Layang Watu Gunung" which dates from the 19th century. Over time, microorganisms can contaminate the paper on the manuscript which can cause damage or biodeterioration. Efforts to preserve historical manuscripts can be started by identifying in advance what types of microorganisms are contaminating the manuscript. From the results of the study, it was found that the microorganisms that contaminate the Layang Watu Gunung Ancient Manuscript are types of bacteria and fungi. For bacteria, the results of the Gram staining test result for contaminant bacteria are Gram Negative Bacteria. This initial research will be the basis for further preservation efforts, the results will be more relevant to be analyzed, especially for other historical manuscripts that are in similar temperature and humidity conditions, namely on the island of Java.
Co-Authors Aisyah Chofifawati Anasom Anasom Anindita Riesti Retno Arimurti Arnia Sari Mukaromah BUDI SETIADI DARYONO Chika, Syifara Dian Aruni Kumalawati Dian Ayuning Tyas Dian Triastari Armanda Feby Kurniawati Hamdan Hadi Kusuma Ismail Ismail Kenari Sri Wahyuni Krisna Wahyu Anggreini Lathifah Nurul Fauzi Lee James Watson Lee James Watson Muhammad Yusrun Niam Nilana Izzati Nova Ulumiya Nugraha, Arifin Budiman Nur Ahmad Nur Hidayatullah Romadhon Riski Apriliyani Robbi Mauizzatul Hikmah Rusmadi Rusmadi Salsabiela Pertiwi Septi Lailia Suknia Umi Cahyaningsih Veenu Kumar Vella Rohmayani Yunita Ayu Ardilla