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deden rosid waltam
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Other

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Journal : Jurnal Bioteknologi

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MUTATION OF LIPASE-PRODUCING BACTERIAL ISOLATE FROM PALM OIL EFFLUENT FOR FAT HYDROLYSIS ON POME dadang suhendar; Ika Rahmatul Layly; Gabriela Christy Sabbathini; Deden Rosid Waltam; Edi Wahjono; Catur Sriherwanto; Haniyya Haniyya
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Lipase is applied to hydrolyzethe residual oil or fat in palm oil mill effluent (POME), which serves as a feedstock for biogas production. A bacterial isolate (Bacillus velezensis) exhibiting lipase activity, previously obtained from Malimping, Pandeglang, Banten, Indonesia, underwentsequential treatments of gamma irradiation (1, 2, and 3 kGy), EMS (ethyl methane sulfonate) mutagenesis (0.5% v/v), and NTG (N-methyl N-nitrosoguanidine) (1 mg mL-1). Following each mutation, lipase activities of the mutant colonies were measured and compared to the wild-type strain. The results revealed that all mutated B. velezensiscolonies exhibited lipase activity values (7.78 ± 0.80 –9.05 ± 0.23 U mL-1) that were not significantly different from the wild-type isolate (8.31 ± 0.01 U mL-1), indicating strong bacterial resistance against the mutagenic treatments. The crude lipase preparation effectively hydrolyzed POME, resulting in a reduction of the COD value (from 131,450 ppm to 88,450 ppm) and O&G content (from 41,400 mg L-1to 5,770 mg L-1) within a 72-hour timeframe.
Co-Authors ahsonul anam Bambang Herlambang Bambang Herlambang, Bambang Budi Kaliwanto Catur Sriherwanto Dadang Suhendar Edi Wahjono Gabriela Christy Sabbathini Haniyya Haniyya Heri Hermansyah Ika Rahmatul Layly Kaliwanto, Budi Siswa Setyahadi Trismilah Trismilah Wawan Budiarto