Garuda - Garba Rujukan Digital

Carbon Steel Biocorrosion Inhibition by Methanol Extract Gracilaria Edulisin Seawater Dukalang, Fildzah Istiqomah; Warganegara, Fida Madayanti; Bundjali, Bunbun
West Science Interdisciplinary Studies Vol. 1 No. 08 (2023): West Science Interdisciplinary Studies
Publisher : Westscience Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58812/wsis.v1i08.149

Biocorossion in terrestrial and marinal habitats has been widely studied including the involvement of sulphate reducing bacteria which are of particular concern in the oil and gas industries. One of the sulphate reducing bacteria that triggers biocorossion is Thiobacillus ferrooxidans. This study examines the effectiveness of Gracilaria edulis macroalgae methanol extract obtained from Sayang Heulang Beach Pamengpeuk District, Garut Regency, West Java as a candidate for carbon steel biocorrosion inhibitors in seawater media. Gracilaria edulis extraction process using Folch extraction method (chloroform: methanol: phosphate buffer, 2: 1: 0.8 (v / v)). Separation of the crude methanol-water phase extract using silica column chromatography G-60 7733 with n-hexane: acetone (8: 2, 5: 5, 2: 8 (v / v)) and methanol p.a. From the results of the analysis using a UV spectrophotometer (190-400 nm) there were five combined fractions (Fraction A-Fraction E). Total content of phenolic compounds (TPC) in crude extracts of methanol-water, fraction C and fraction E respectively (410.77; 285.30; 309.51) (mg/gr GAE). The results of the qualitative test of the ability of biocorrosion inhibition of crude extracts of methanol-water phase, fraction C and fraction E by using commercial nails in solid media incubated for four days showed a decrease in the corrosion intensity on nails. Quantitative tests on the ability of biocorrosion inhibition were carried out using the weight-loss method and the results of quantitative tests showed the corrosion rate of fraction C was 12.14 ± 1.09 mpy and the fraction E was 13.94 ± 1.82 mpy for 2.5% NaCl medium while the corrosion rate of fraction C was 10.73 ± 3.63 mpy and the fraction E was 11.72 ± 0.59 mpy for seawater media. The optimum concentration test results in the inhibition showed the smallest corrosion rate fraction C was found at 0.2 mg / mL TPC levels both in 2.5% NaCl media and seawater media which each had a corrosion rate of 12.91 mpy and 8.09 mpy and the optimum concentration of fraction E was found in TPC levels of 0.4 mg / mL (2.5% NaCl media) with a corrosion rate of 14.32 mpy and TPC levels of 0.1 mg / mL (sea water medium) with a corrosion rate of 12.14 mpy. By using the optimum fraction C concentration, the results showed that the fraction C had the maximum resistance in inhibiting for 8 days (192 hours). Analysis of carbon steel plates using SEM and EDS showed that T. ferrooxidans bacterial cells formed a biofilm layer on the surface of the carbon steel plate with a localized density but mostly dominated the carbon steel plate surface and by the addition of SEM image inhibitors showed the carbon steel plate forming a layer thin that protects the metal surface and decreases the solubility of iron sulfide in water. The test results using EDS devices also detect the presence of sulfur elements and a decrease in the percentage of iron element mass on carbon steel plates without inhibitors.

Carbon Steel Biocorrosion Inhibition by Methanol Extract Gracilaria Edulisin Seawater Dukalang, Fildzah Istiqomah; Warganegara, Fida Madayanti; Bundjali, Bunbun
West Science Interdisciplinary Studies Vol. 1 No. 08 (2023): West Science Interdisciplinary Studies
Publisher : Westscience Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58812/wsis.v1i08.149

Biocorossion in terrestrial and marinal habitats has been widely studied including the involvement of sulphate reducing bacteria which are of particular concern in the oil and gas industries. One of the sulphate reducing bacteria that triggers biocorossion is Thiobacillus ferrooxidans. This study examines the effectiveness of Gracilaria edulis macroalgae methanol extract obtained from Sayang Heulang Beach Pamengpeuk District, Garut Regency, West Java as a candidate for carbon steel biocorrosion inhibitors in seawater media. Gracilaria edulis extraction process using Folch extraction method (chloroform: methanol: phosphate buffer, 2: 1: 0.8 (v / v)). Separation of the crude methanol-water phase extract using silica column chromatography G-60 7733 with n-hexane: acetone (8: 2, 5: 5, 2: 8 (v / v)) and methanol p.a. From the results of the analysis using a UV spectrophotometer (190-400 nm) there were five combined fractions (Fraction A-Fraction E). Total content of phenolic compounds (TPC) in crude extracts of methanol-water, fraction C and fraction E respectively (410.77; 285.30; 309.51) (mg/gr GAE). The results of the qualitative test of the ability of biocorrosion inhibition of crude extracts of methanol-water phase, fraction C and fraction E by using commercial nails in solid media incubated for four days showed a decrease in the corrosion intensity on nails. Quantitative tests on the ability of biocorrosion inhibition were carried out using the weight-loss method and the results of quantitative tests showed the corrosion rate of fraction C was 12.14 ± 1.09 mpy and the fraction E was 13.94 ± 1.82 mpy for 2.5% NaCl medium while the corrosion rate of fraction C was 10.73 ± 3.63 mpy and the fraction E was 11.72 ± 0.59 mpy for seawater media. The optimum concentration test results in the inhibition showed the smallest corrosion rate fraction C was found at 0.2 mg / mL TPC levels both in 2.5% NaCl media and seawater media which each had a corrosion rate of 12.91 mpy and 8.09 mpy and the optimum concentration of fraction E was found in TPC levels of 0.4 mg / mL (2.5% NaCl media) with a corrosion rate of 14.32 mpy and TPC levels of 0.1 mg / mL (sea water medium) with a corrosion rate of 12.14 mpy. By using the optimum fraction C concentration, the results showed that the fraction C had the maximum resistance in inhibiting for 8 days (192 hours). Analysis of carbon steel plates using SEM and EDS showed that T. ferrooxidans bacterial cells formed a biofilm layer on the surface of the carbon steel plate with a localized density but mostly dominated the carbon steel plate surface and by the addition of SEM image inhibitors showed the carbon steel plate forming a layer thin that protects the metal surface and decreases the solubility of iron sulfide in water. The test results using EDS devices also detect the presence of sulfur elements and a decrease in the percentage of iron element mass on carbon steel plates without inhibitors.

Carbon Steel Biocorrosion Inhibition by Methanol Extract Gracilaria Edulisin Seawater Dukalang, Fildzah Istiqomah; Warganegara, Fida Madayanti; Bundjali, Bunbun
West Science Interdisciplinary Studies Vol. 1 No. 08 (2023): West Science Interdisciplinary Studies
Publisher : Westscience Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58812/wsis.v1i08.149

Biocorossion in terrestrial and marinal habitats has been widely studied including the involvement of sulphate reducing bacteria which are of particular concern in the oil and gas industries. One of the sulphate reducing bacteria that triggers biocorossion is Thiobacillus ferrooxidans. This study examines the effectiveness of Gracilaria edulis macroalgae methanol extract obtained from Sayang Heulang Beach Pamengpeuk District, Garut Regency, West Java as a candidate for carbon steel biocorrosion inhibitors in seawater media. Gracilaria edulis extraction process using Folch extraction method (chloroform: methanol: phosphate buffer, 2: 1: 0.8 (v / v)). Separation of the crude methanol-water phase extract using silica column chromatography G-60 7733 with n-hexane: acetone (8: 2, 5: 5, 2: 8 (v / v)) and methanol p.a. From the results of the analysis using a UV spectrophotometer (190-400 nm) there were five combined fractions (Fraction A-Fraction E). Total content of phenolic compounds (TPC) in crude extracts of methanol-water, fraction C and fraction E respectively (410.77; 285.30; 309.51) (mg/gr GAE). The results of the qualitative test of the ability of biocorrosion inhibition of crude extracts of methanol-water phase, fraction C and fraction E by using commercial nails in solid media incubated for four days showed a decrease in the corrosion intensity on nails. Quantitative tests on the ability of biocorrosion inhibition were carried out using the weight-loss method and the results of quantitative tests showed the corrosion rate of fraction C was 12.14 ± 1.09 mpy and the fraction E was 13.94 ± 1.82 mpy for 2.5% NaCl medium while the corrosion rate of fraction C was 10.73 ± 3.63 mpy and the fraction E was 11.72 ± 0.59 mpy for seawater media. The optimum concentration test results in the inhibition showed the smallest corrosion rate fraction C was found at 0.2 mg / mL TPC levels both in 2.5% NaCl media and seawater media which each had a corrosion rate of 12.91 mpy and 8.09 mpy and the optimum concentration of fraction E was found in TPC levels of 0.4 mg / mL (2.5% NaCl media) with a corrosion rate of 14.32 mpy and TPC levels of 0.1 mg / mL (sea water medium) with a corrosion rate of 12.14 mpy. By using the optimum fraction C concentration, the results showed that the fraction C had the maximum resistance in inhibiting for 8 days (192 hours). Analysis of carbon steel plates using SEM and EDS showed that T. ferrooxidans bacterial cells formed a biofilm layer on the surface of the carbon steel plate with a localized density but mostly dominated the carbon steel plate surface and by the addition of SEM image inhibitors showed the carbon steel plate forming a layer thin that protects the metal surface and decreases the solubility of iron sulfide in water. The test results using EDS devices also detect the presence of sulfur elements and a decrease in the percentage of iron element mass on carbon steel plates without inhibitors.

Screening and Isolation of Thermophilic Amylase-Producing Bacteria from Ulubelu Hot Springs in Tanggamus Lampung Frima, Fina Khaerunnisa; Dewi, Fransiska Riana; Putri, Syahdila Ramadianti Eka; Ayuwulanda, Aditya; Ulpiyana, Ayra; Warganegara, Fida Madayanti
Jurnal Kartika Kimia Vol 8 No 2 (2025): Jurnal Kartika Kimia (In Progress)
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v8i2.966

Thermophilic bacteria generally thrive in hot springs and produce thermostable enzymes. These enzymes retain catalytic activity at high temperatures and play an important role in various industrial applications. This study aims to conduct screening, isolation, and characterization of thermophilic amylase-producing bacteria from the Ulubelu hot spring located in Tanggamus Regency, Lampung. Hot spring water samples from a location with a temperature of 60°C and pH 5 yielded eight bacterial isolates with amylolytic activity, as indicated by clear zones around the colonies. Among the isolates obtained, Ulb A3.7 exhibited the largest clear zone diameter and was therefore selected for further characterization. Morphological and physiological analyses revealed that Ulb A3.7 is a Gram-negative rod-shaped bacterium. The Ulb A3.7 isolate tested positive for oxidative-fermentative metabolism, oxidase activity, and glucose utilization. The crude α-amylase from Ulb A3.7 exhibited optimal activity at 80°C and demonstrated a broad activity range. The enzyme was able to retain its amylolytic activity for up to 300 minutes of incubation, indicating its potential for application in high-temperature industrial processes.

Computational Study on Apoptosis-Inducing Factor (AIF)-Mediated Modulation of Menadione using Molecular Docking and Parametrized Quantum Methods Listyawati, Muklisatum; Hildayani, Suci Zulaikha; Ledyastuti, Mia; Warganegara, Fida Madayanti; Martoprawiro, Muhamad Abdulkadir
Molekul Vol 20 No 3 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.3.12428

ABSTRACT. Apoptosis-inducing factor (AIF) is a protein that is crucial for apoptosis which its dysregulation has been connected to the emergence of cancer. Mitochondria are organelles that in healthy cells function as energy factories that are important for maintaining cell life. AIF is located in the mitochondrial intermembrane space with active sites, namely FAD and NADH. Meanwhile in the recent studies, quinone compounds have shown potential as anti-cancer agents by targeting mitochondrial function, but the mechanism is still unclear. In this study, we used computational methods, including molecular docking and the Divide-and-Conquer Density Functional Tight-Binding Molecular Dynamics (DCDFTBMD) method, a type of parametrized quantum calculation, to investigate the interaction between mitochondrial AIF and menadione, as a quinone compound with anticancer potential. From the calculation result, AIF interaction with menadione was stronger in the FAD domain than in NADH. The partial charges of the ligands before and after the interactions were calculated using the DCDFTBMD technique, and the results show that the charge in the bonding area becomes more negative. This indicates the strengthening of electrostatic interactions through polarization effects in the bonding molecule. Additionally, it was discovered through this study's data that all ligands interacted with the ser-175 residue in the AIF protein. These residues are modulated by the involvement of AIF in the cell death process. From this study, it can be concluded that menadione has the potential as an anticancer agent through the AIF modulation mechanism. Keywords: AIF, enzyme-catalyzed modulation, docking, quantum parametrization, menadione

Title

Found 5 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 5 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 5 Documents
Search