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All Journal Jurnal Biologi dan Pembelajarannya (JBP) Biology, Medicine, & Natural Product Chemistry BIOMA : Jurnal Biologi Makassar Martabe : Jurnal Pengabdian Kepada Masyarakat BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains KLOROFIL: Jurnal Ilmu Biologi dan Terapan Bioma : Jurnal Biologi dan Pembelajaran Biologi Best Journal (Biology Education, Sains and Technology) KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Teknik dan Science (JTS) Journal of Pharmaceutical and Sciences.
Rizki Amelia Nasution
Universitas Islam Negeri Sumatera Utara
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences

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Uji Aktivitas Antimikroba Ekstrak Daun Randu (Ceiba pentandra L.) Terhadap Pertumbuhan Pseudomonas aeruginosa dan Candida albicans Misriani Ani; Kartika Manalu; Rizki Amelia Nasution
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.13740

Abstract

One of the traditional plants used by the community is the kapok plant (Ceiba pentandra L.) apart from being cultivated, parts of the kapok plant are also used as building materials, stuffing for mattresses and traditional medicine. The purpose of this study was to determine the activity of randu leaf extract on the growth of Pseudomonas aeruginosa and Candida albicans. This study used a completely randomized design (CRD) with 6 treatment groups, namely the positive control group with chloramphenicol and ketoconazole. The negative control group used 10% DMSO, and the extract concentration group was 70%, 80%, 90% and 100%. The steps carried out in this study included the extraction of kapok leaves using 96% ethanol as solvent, phytochemical screening and antimicrobial testing using the Kirby-Bauer Disc Diffusion method. Analysis of research data using SPSS 21. From the results of this study, it was found that randu leaf extract contained alkaloids, saponins and tannins. Antimicrobial testing with the kapok leaf extract group resulted in an average inhibition zone of 7.57 mm, 8.0 mm, 8.7 mm, and 9.87 mm against Pseudomonas aeruginosa and 4.67 mm, 5.07 mm, 5.32 mm, and 5.65 mm against Candida albicans. The results of the One Way ANOVA test show that Ftable < Fcount which proves that the leaf extract of Randu provides antimicrobial activity in inhibiting the growth of Pseudomonas aeruginosa and Candida albicans.
UJI AKTIVITAS MINYAK ATSIRI DAUN EKOR KUCING (Acalypha hispida) SEBAGAI DISINFEKTAN ALAMI TERHADAP BAKTERI Bacillus subtilis DAN Klebsiella pneumoniae SECARA IN- VITRO Nurlayly Zaini; Ulfayani Mayasari; Rizki Amelia Nasution
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 1 (2024): Bioma : Januari - Juni 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bakteri Bacillus subtilis dan Klebsiella pneumoniae merupakan bakteri yang tersebar di udara, air dan tanah yang dapat menyebabkan penyakit infeksi meningitis. Penggunaan disinfektan alami minyak atsiri daun ekor kucing (Acalypha hispida) yang mengandung senyawa antibakteri flavonoid dan tanin akan menghambat pertumbuhan bakteri. Penelitian ini bertujuan untuk mengetahui aktivitas disinfektan alami minyak atsiri daun ekor kucing (Acalypha hispida) terhadap pertumbuhan bakteri Bacillus subtilis dan Klebsiella pneumoniae secara In-Vitro. Hasil uji aktivitas disinfektan alami minyak atsiri daun ekor kucing (Acalypha hispida) menggunakan konsentrasi 2,5%, 5%, 7,5%, dan 10%, diperoleh hasil diameter zona hambat pada bakteri Bacillus subtilis yaitu 17,54, 14,13, 13,66, dan 11,7 sedangkan hasil uji diameter zona hambat pada bakteri Klebsiella pneumonia yaitu 19,00, 16,57, 14,47, dan 12,59. Berdasarkan hasil dari rata-rata aktivitas antibakteri zona hambat minyak atsiri daun ekor kucing (Acalypha hispida) dapat dijadikan sebagai disinfektan alami terhadap bakteri Bacillus subtilis pada konsentrasi efektif 5%, sedangkan bakteri Klebsiella pneumoniae pada konsentrasi efektif 7,5%. Kata kunci: Minyak atsiri, disinfektan, Bacillus subtilis dan Klebsiella pneumoniae
Isolasi dan Identifikasi Bakteri Asam Laktat dari Hasil Fermentasi Limbah Organik Kulit Buah (Eco-Enzyme) Linda Syafitri Siagian; Kartika Manalu; Rizki Amelia Nasution
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 1 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i1.9967

Abstract

The aim of this research is the isolation and identification based on the morphological characteristics of lactic acid bacteria in the fermentation of organic waste from fruit peels. Isolation of Lactic Acid Bacteria was carried out using the spread plate method on de Man, Rogosa, and Sharpe Agar (MRSA) media. The culture was incubated at 37 °C for 48 hours. The growing colonies were observed for morphological characteristics, and bacterial Gram staining was carried out. The results of the research showed that 10 bacterial isolates were obtained from the isolation of BAL from ecoenzymes on de Man, Ragosa, and Sharpe Agar (MRSA) media, namely E-BAL 01, E-BAL 02, E-BAL O3, E-BAL 04, E-BAL 05, E-BAL 06, E-BAL 07, E-BAL 08, E-BAL 09, and E-BAL 10, and 10 isolates were characterized as Gram-positive with a bacillary cell form. In conclusion, 10 isolates were found with characteristics that supported MRSA media, namely isolates of lactic acid bacteria of the genus Lactobacillus. This is confirmed by the results of biochemical tests, namely that it is immobile, does not produce the catalase enzyme, and does not have the ability to produce gas. Keywords: Lactic Acid Bacteria (LAB), Ecoenzymes, Fruit Skin
Isolasi dan Identifikasi Bakteri Endofit Penghasil Hormon IAA (Indole Acetic Acid) dari Akar Tanaman Mangrove Avicennia marina Suci Ramadani Pohan; Kartika Manalu; Rizki Amelia Nasution
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 1 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i1.9968

Abstract

This research aims to determine the genus and potential isolates of endophytic bacteria producing IAA hormone from the roots of mangrove plants in Tanjung Rejo village, Percut Sai Tuan subdistrict. The research methods used include isolating endophytic bacteria using the plant method in NA (Nutrient Agar) media then culturing them in NB (Nutrient Broth) media then adding L-tryptophan and incubating, then identification is carried out based on morphological characterization, gram staining and biochemistry. The results of the research showed that 13 isolates of endophytic bacteria were found that had the potential to produce IAA hormones ranging from 0.047-0.050 ppm which were identified as the genus Enterobacter, Pseudomonas, Bacillus, Azosprilium, Micrococcus. However, the isolates BEAM PA 2 of the genus Enterobacter and BPAM PE 6 of the genus Micrococcus had the highest value of 0.050 ppm. The conclusion from this research is that the endophytic bacteria obtained as a whole have the ability to produce the hormone IAA. Keywords: Endophytic Bacteria, IAA (Indole Acetic Acid), Mangrove Avicennia marina
PEMBUATAN MEDIA PERTUMBUHAN BAKTERI ESCHERICHIA COLI DENGAN MENGGUNAKAN UMBI UBI JALAR PUTIH IPOMOEA BATATAS Ita Hasanah Br Karo; Rasyidah Rasyidah; Rizki Amelia Nasution
Jurnal Teknik dan Science Vol. 3 No. 3 (2024): Oktober: Jurnal Teknik dan Science
Publisher : Asosiasi Dosen Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56127/jts.v3i3.1894

Abstract

Nutrient Agar (NA) is a growth medium for heterotrophic microorganisms made from meat extract, peptone and agar, but commercially it is relatively expensive. As an alternative, researchers innovated using white sweet potatoes (Ipomoea batatas) which are rich in carbohydrates, protein, fat and natural fiber as a growth medium for Escherichia coli bacteria. The aim of this research is to examine the use of white sweet potato flour as a growth medium for Escherichia coli bacteria, and compare it with a medium that is added with meat broth. This research used the experimental research method twice with three repetitions of sweet potato flour, namely 10gr (w/v), 15gr (w/v), 20gr (w/v) and NA control. After conducting research on white sweet potato flour, it was found that the water content test results met the standards of around 8.56–9.66%. The panelists' organoleptic test stated that white sweet potato flour had a smooth texture, yellowish white color, slightly sweet aroma. The solubility test proved that white sweet potato flour does not dissolve in water, and the pH test showed a value of 6.8, the percentage of carbohydrates contained was 74.32%, and protein 1.36%. The results obtained by the number of bacterial colonies with the best treatment without broth were F3 (20 gr white sweet potato flour) 25.5 x 106 CFU and the best treatment with the addition of broth was F2 (sweet potato flour + 15 gr broth) 28.7 x 106 while the NA control showed results of 25.6 x 106 CFU. It can be concluded that white sweet potato flour meets all test standards so that it can be used as an alternative growth medium for bacteria, especially Escherichia coli.
Formulasi Sediaan Sheet Mask Ekstrak Daun Murbei (Morus alba L.) sebagai Anti Acne Terhadap Bakteri Propionibacterium acnes Nurul Aina Harahap; Ulfayani Mayasari; Rizki Amelia Nasution
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 7 No. 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.13000

Abstract

This study aimed to determine the antibacterial activity of mulberry leaf extract and formulate Sheet Masks containing the extract at different concentrations of 4%, 6%, 8%, and 10%. The method involved antibacterial activity testing using the disk diffusion method with positive control (clindamycin and Wardah Sheet Mask), negative control (DMSO), and three replications. Organoleptic and physical evaluations of the Sheet Mask formulation were also conducted. The results showed that mulberry leaf extract inhibited the growth of Propionibacterium acnes, with inhibition zones of 6.0 mm at 4%, 9.45 mm at 6%, 12.57 mm at 8%, and 13.95 mm at 10%. For the Sheet Mask formulations, the inhibition zones were 10.69 mm for the positive control (clindamycin), 0.1 mm for F0, 9.78 mm for F1 (8%), and 10.29 mm for F2 (10%). Organoleptic evaluation revealed that the most preferred formulations in terms of color, aroma, and texture were F0, F2, and F1, respectively. Physical evaluation confirmed that the Sheet Mask formulations met quality standards. In conclusion, mulberry leaf extract demonstrated antibacterial activity against Propionibacterium acnes, with the most effective concentrations being 8% and 10%. Keywords: antibacterial, acne, Sheet Mask, mulberry, Propionibacterium acnes
Efektivitas Salep Ekstrak Etanol Cengkeh (Syzygium aromaticum L) dalam Menghambat Pertumbuhan Bakteri Penyebab Infeksi Kulit Vara Nabila; Rizki Amelia Nasution; Ulfayani Mayasari
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 7 No. 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.13002

Abstract

This study aims to determine whether clove ethanol extract can be formulated into ointment preparations, evaluate the effectiveness of the ointment in inhibiting bacterial growth causing skin infections, and identify the effective concentration of clove ethanol extract ointment formulation against Streptococcus pyogenes and Pseudomonas aeruginosa. The methods used were disk diffusion and well diffusion with clove ethanol extract concentrations of 10%, 20%, and 30%. Chloramphenicol served as a positive control, and distilled water as a negative control. The ointment formulation was tested at effective doses of 10% and 30% as per Pharmacopeia guidelines. The positive control used bactoderm ointment, and the negative control was an ointment base without clove extract. The results showed that clove ethanol extract could be formulated into an ointment after undergoing phytochemical screening. The clove ethanol extract ointment effectively inhibited bacterial growth causing skin infections. The inhibition zones for Streptococcus pyogenes were 12.16 mm and 14.06 mm, while for Pseudomonas aeruginosa they were 10.16 mm and 9.3 mm. The effective concentrations of clove ethanol extract ointment in inhibiting bacterial growth were 10% and 30%. Keywords: Bacteria, Skin Infection, Clove Ointment
Trichoderma sp. Mushroom Optimization with the Addition of Biostimulants to Reduce TPH (Total Petroleum Hydrocarbons) Levels in Soil Polluted by Palm Oil Mill Waste Intan Nurhaliza Pane; Rizki Amelia Nasution
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1617-1623

Abstract

Benzene and toluene compounds in aromatic hydrocarbons can be found from the results of the liquid waste treatment process of palm oil mills that have the potential to pollute the environment in the soil ecosystem. This study aimed to optimize the fungus Trichoderma sp. by adding biostimulants to reduce TPH levels in soil contaminated with palm oil mill waste. The method used in reducing TPH levels was carried out by gravimetric analysis with the addition of biostimulant concentrations in the form of P0 (control), P1 (Trichoderma sp. 15%), P2 (Trichoderma sp. 15% + 35 gr rice husks), P3 (Trichoderma sp. 15% + 100 gr cow dung), P4 (Trichoderma sp. 15% + 35 gr rice husks + 100 gr cow dung) on 300 gr of soil contaminated with oil palm mill liquid waste,  nitrogen content test using the Kjeldahl method, phosphorus content test using Spectrophotometry method, potassium content test using AAS method, and pH test. The results showed that the optimization of the fungus Trichoderma sp. with the addition of 35 grams of rice husk stimulant degraded TPH levels by 64.11%, the addition of 100 grams of cow manure stimulant degraded TPH levels by 66.92%, the combined addition of 35 grams of rice husk stimulant and 100 grams of cow manure showed a degradation of TPH levels by 76.02%, and treatment without stimulants degraded TPH levels by 44.90%. Optimization of the fungus Trichoderma sp. with the addition of stimulants in the form of rice husks and cow manure has a significant impact on the reduction of TPH levels.
Analisis Kualitas Yogurt Santan Kelapa Dengan Penambahan Tepung Kolang Kaling Selama Penyimpanan Rizki Amelia Nasution; Efrida Pima Sari Tambunan
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1298

Abstract

The addition of flour from kolang kaling in this research aims to act as a stabilizer and improve the quality of fermented drinks (yoghurt) made from coconut milk. This research aims to analyze the quality of coconut milk yogurt with the addition of kolang kaling flour by testing its physicochemical properties. Experimental research with coconut milk yogurt samples with the addition of 1% kolang kaling flour and without the addition was stored at 40C for 14 days and analyzed for pH, viscosity and total LAB, as well as testing the research samples for water content using the distillation method (thermovolumetric), protein using the method Kjeldahl, fat using Soxhlet extraction, carbohydrate (starch) content testing using the UV Visible spectrophotometric method, and total acid using the titration and organoleptic method with 9 hedonic scales. Research shows that the results of adding palm fruit flour have an effect on the analysis parameters and are in accordance with SNI, namely pH reaches 5-3.94, carbohydrates decrease during H14 fermentation by 20.1%, protein reaches 6.5%, fat around 3%, water content is very low, namely 49%, The viscosity is very low, namely 2.8cP, the acid number is sufficient according to SNI in H14, namely 2%, %, as well as the hedonic results during storage which show statistical test results which are significantly different (P<0.05) on day 7 and day 14. The research can be concluded based on physicochemical analysis and conformity with SNI that the quality of coconut milk yogurt with the addition of kolang kaling flour during storage is good and in accordance with food standards.
Isolasi dan Identifikasi Bakteri Fiksasi Nitrogen Dari Akar Padi Hidroponik Dan Akar Padi Konvensional Pinkhan Aliffia Sekar Andini; Kartika Manalu; Rizki Amelia Nasution
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 11 No 2 (2024): Oktober 2024
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v11i2.22304

Abstract

Nitrogen fixation bacteria are bacteriathat are able to convert free nitrogen into nitrogen that can be used by theplants. This study aims to determine the presence of bacteria fixationnitrogen fixation bacteria in hydroponic and conventional rice roots and can identify the bacteriaidentify the bacteria. Isolation was done from the roots of rice (Oryza sativa) with the scatter blot method on NA (Nutriet Agar), and then tested for thethen tested for the ability to fix nitrogen using mineral-free nitrogen media added with Brontomimineral-free nitrogen medium added with Brontomiul Blue and glucose, which was incubated for 24 hours, thenincubated for 24 hours and then biochemical tests. From the results of the isolation there are 2 isolates that are able to bind nitrogen and have the ability ofHOS 833 ppm and KOS 1,128 ppm. The results of the characteristics and biochemistrycharacteristics and biochemistry showed that the isolate HOS is the genusBacillus genus and KOS is Azotobacter genus.
Co-Authors Almadina Septiyah Asti Hanipah Efrida Pima Sari Tambunan Intan Nurhaliza Pane Ita Hasanah Br Karo Kartika Manalu Linda Syafitri Siagian Misriani Ani Nur Ade Erma Nia Nurlayly Zaini Nurul Aina Harahap Pinkhan Aliffia Sekar Andini Rasyidah Riris Silvia Tampubolon S.Pd. M Kes I Ketut Sudiana . Suci Ramadani Pohan Suci Ramadhani Ulfayani Mayasari Vara Nabila Zahratul Idami Zulita Mega Anggriany