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All Journal BIOMA : Jurnal Biologi Makassar Martabe : Jurnal Pengabdian Kepada Masyarakat BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains KLOROFIL: Jurnal Ilmu Biologi dan Terapan Bioma : Jurnal Biologi dan Pembelajaran Biologi Best Journal (Biology Education, Sains and Technology) KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal IPTEK Bagi Masyarakat Jurnal Teknik dan Science (JTS)
Rizki Amelia Nasution
Universitas Islam Negeri Sumatera Utara
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Education Electrical & Electronics Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Physics Public Health Social Sciences

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Uji Aktivitas Antimikroba Ekstrak Daun Randu (Ceiba pentandra L.) Terhadap Pertumbuhan Pseudomonas aeruginosa dan Candida albicans Misriani Ani; Kartika Manalu; Rizki Amelia Nasution
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.13740

Abstract

One of the traditional plants used by the community is the kapok plant (Ceiba pentandra L.) apart from being cultivated, parts of the kapok plant are also used as building materials, stuffing for mattresses and traditional medicine. The purpose of this study was to determine the activity of randu leaf extract on the growth of Pseudomonas aeruginosa and Candida albicans. This study used a completely randomized design (CRD) with 6 treatment groups, namely the positive control group with chloramphenicol and ketoconazole. The negative control group used 10% DMSO, and the extract concentration group was 70%, 80%, 90% and 100%. The steps carried out in this study included the extraction of kapok leaves using 96% ethanol as solvent, phytochemical screening and antimicrobial testing using the Kirby-Bauer Disc Diffusion method. Analysis of research data using SPSS 21. From the results of this study, it was found that randu leaf extract contained alkaloids, saponins and tannins. Antimicrobial testing with the kapok leaf extract group resulted in an average inhibition zone of 7.57 mm, 8.0 mm, 8.7 mm, and 9.87 mm against Pseudomonas aeruginosa and 4.67 mm, 5.07 mm, 5.32 mm, and 5.65 mm against Candida albicans. The results of the One Way ANOVA test show that Ftable < Fcount which proves that the leaf extract of Randu provides antimicrobial activity in inhibiting the growth of Pseudomonas aeruginosa and Candida albicans.
UJI AKTIVITAS MINYAK ATSIRI DAUN EKOR KUCING (Acalypha hispida) SEBAGAI DISINFEKTAN ALAMI TERHADAP BAKTERI Bacillus subtilis DAN Klebsiella pneumoniae SECARA IN- VITRO Nurlayly Zaini; Ulfayani Mayasari; Rizki Amelia Nasution
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 1 (2024): Bioma : Januari - Juni 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bakteri Bacillus subtilis dan Klebsiella pneumoniae merupakan bakteri yang tersebar di udara, air dan tanah yang dapat menyebabkan penyakit infeksi meningitis. Penggunaan disinfektan alami minyak atsiri daun ekor kucing (Acalypha hispida) yang mengandung senyawa antibakteri flavonoid dan tanin akan menghambat pertumbuhan bakteri. Penelitian ini bertujuan untuk mengetahui aktivitas disinfektan alami minyak atsiri daun ekor kucing (Acalypha hispida) terhadap pertumbuhan bakteri Bacillus subtilis dan Klebsiella pneumoniae secara In-Vitro. Hasil uji aktivitas disinfektan alami minyak atsiri daun ekor kucing (Acalypha hispida) menggunakan konsentrasi 2,5%, 5%, 7,5%, dan 10%, diperoleh hasil diameter zona hambat pada bakteri Bacillus subtilis yaitu 17,54, 14,13, 13,66, dan 11,7 sedangkan hasil uji diameter zona hambat pada bakteri Klebsiella pneumonia yaitu 19,00, 16,57, 14,47, dan 12,59. Berdasarkan hasil dari rata-rata aktivitas antibakteri zona hambat minyak atsiri daun ekor kucing (Acalypha hispida) dapat dijadikan sebagai disinfektan alami terhadap bakteri Bacillus subtilis pada konsentrasi efektif 5%, sedangkan bakteri Klebsiella pneumoniae pada konsentrasi efektif 7,5%. Kata kunci: Minyak atsiri, disinfektan, Bacillus subtilis dan Klebsiella pneumoniae
Isolasi dan Identifikasi Bakteri Asam Laktat dari Hasil Fermentasi Limbah Organik Kulit Buah (Eco-Enzyme) Linda Syafitri Siagian; Kartika Manalu; Rizki Amelia Nasution
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 1 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i1.9967

Abstract

The aim of this research is the isolation and identification based on the morphological characteristics of lactic acid bacteria in the fermentation of organic waste from fruit peels. Isolation of Lactic Acid Bacteria was carried out using the spread plate method on de Man, Rogosa, and Sharpe Agar (MRSA) media. The culture was incubated at 37 °C for 48 hours. The growing colonies were observed for morphological characteristics, and bacterial Gram staining was carried out. The results of the research showed that 10 bacterial isolates were obtained from the isolation of BAL from ecoenzymes on de Man, Ragosa, and Sharpe Agar (MRSA) media, namely E-BAL 01, E-BAL 02, E-BAL O3, E-BAL 04, E-BAL 05, E-BAL 06, E-BAL 07, E-BAL 08, E-BAL 09, and E-BAL 10, and 10 isolates were characterized as Gram-positive with a bacillary cell form. In conclusion, 10 isolates were found with characteristics that supported MRSA media, namely isolates of lactic acid bacteria of the genus Lactobacillus. This is confirmed by the results of biochemical tests, namely that it is immobile, does not produce the catalase enzyme, and does not have the ability to produce gas. Keywords: Lactic Acid Bacteria (LAB), Ecoenzymes, Fruit Skin
Isolasi dan Identifikasi Bakteri Endofit Penghasil Hormon IAA (Indole Acetic Acid) dari Akar Tanaman Mangrove Avicennia marina Suci Ramadani Pohan; Kartika Manalu; Rizki Amelia Nasution
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 1 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i1.9968

Abstract

This research aims to determine the genus and potential isolates of endophytic bacteria producing IAA hormone from the roots of mangrove plants in Tanjung Rejo village, Percut Sai Tuan subdistrict. The research methods used include isolating endophytic bacteria using the plant method in NA (Nutrient Agar) media then culturing them in NB (Nutrient Broth) media then adding L-tryptophan and incubating, then identification is carried out based on morphological characterization, gram staining and biochemistry. The results of the research showed that 13 isolates of endophytic bacteria were found that had the potential to produce IAA hormones ranging from 0.047-0.050 ppm which were identified as the genus Enterobacter, Pseudomonas, Bacillus, Azosprilium, Micrococcus. However, the isolates BEAM PA 2 of the genus Enterobacter and BPAM PE 6 of the genus Micrococcus had the highest value of 0.050 ppm. The conclusion from this research is that the endophytic bacteria obtained as a whole have the ability to produce the hormone IAA. Keywords: Endophytic Bacteria, IAA (Indole Acetic Acid), Mangrove Avicennia marina
PEMBUATAN MEDIA PERTUMBUHAN BAKTERI ESCHERICHIA COLI DENGAN MENGGUNAKAN UMBI UBI JALAR PUTIH IPOMOEA BATATAS Ita Hasanah Br Karo; Rasyidah Rasyidah; Rizki Amelia Nasution
Jurnal Teknik dan Science Vol. 3 No. 3 (2024): Oktober: Jurnal Teknik dan Science
Publisher : Asosiasi Dosen Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56127/jts.v3i3.1894

Abstract

Nutrient Agar (NA) is a growth medium for heterotrophic microorganisms made from meat extract, peptone and agar, but commercially it is relatively expensive. As an alternative, researchers innovated using white sweet potatoes (Ipomoea batatas) which are rich in carbohydrates, protein, fat and natural fiber as a growth medium for Escherichia coli bacteria. The aim of this research is to examine the use of white sweet potato flour as a growth medium for Escherichia coli bacteria, and compare it with a medium that is added with meat broth. This research used the experimental research method twice with three repetitions of sweet potato flour, namely 10gr (w/v), 15gr (w/v), 20gr (w/v) and NA control. After conducting research on white sweet potato flour, it was found that the water content test results met the standards of around 8.56–9.66%. The panelists' organoleptic test stated that white sweet potato flour had a smooth texture, yellowish white color, slightly sweet aroma. The solubility test proved that white sweet potato flour does not dissolve in water, and the pH test showed a value of 6.8, the percentage of carbohydrates contained was 74.32%, and protein 1.36%. The results obtained by the number of bacterial colonies with the best treatment without broth were F3 (20 gr white sweet potato flour) 25.5 x 106 CFU and the best treatment with the addition of broth was F2 (sweet potato flour + 15 gr broth) 28.7 x 106 while the NA control showed results of 25.6 x 106 CFU. It can be concluded that white sweet potato flour meets all test standards so that it can be used as an alternative growth medium for bacteria, especially Escherichia coli.
Formulasi Sediaan Sheet Mask Ekstrak Daun Murbei (Morus alba L.) sebagai Anti Acne Terhadap Bakteri Propionibacterium acnes Nurul Aina Harahap; Ulfayani Mayasari; Rizki Amelia Nasution
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 7 No. 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.13000

Abstract

This study aimed to determine the antibacterial activity of mulberry leaf extract and formulate Sheet Masks containing the extract at different concentrations of 4%, 6%, 8%, and 10%. The method involved antibacterial activity testing using the disk diffusion method with positive control (clindamycin and Wardah Sheet Mask), negative control (DMSO), and three replications. Organoleptic and physical evaluations of the Sheet Mask formulation were also conducted. The results showed that mulberry leaf extract inhibited the growth of Propionibacterium acnes, with inhibition zones of 6.0 mm at 4%, 9.45 mm at 6%, 12.57 mm at 8%, and 13.95 mm at 10%. For the Sheet Mask formulations, the inhibition zones were 10.69 mm for the positive control (clindamycin), 0.1 mm for F0, 9.78 mm for F1 (8%), and 10.29 mm for F2 (10%). Organoleptic evaluation revealed that the most preferred formulations in terms of color, aroma, and texture were F0, F2, and F1, respectively. Physical evaluation confirmed that the Sheet Mask formulations met quality standards. In conclusion, mulberry leaf extract demonstrated antibacterial activity against Propionibacterium acnes, with the most effective concentrations being 8% and 10%. Keywords: antibacterial, acne, Sheet Mask, mulberry, Propionibacterium acnes
Efektivitas Salep Ekstrak Etanol Cengkeh (Syzygium aromaticum L) dalam Menghambat Pertumbuhan Bakteri Penyebab Infeksi Kulit Vara Nabila; Rizki Amelia Nasution; Ulfayani Mayasari
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 7 No. 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.13002

Abstract

This study aims to determine whether clove ethanol extract can be formulated into ointment preparations, evaluate the effectiveness of the ointment in inhibiting bacterial growth causing skin infections, and identify the effective concentration of clove ethanol extract ointment formulation against Streptococcus pyogenes and Pseudomonas aeruginosa. The methods used were disk diffusion and well diffusion with clove ethanol extract concentrations of 10%, 20%, and 30%. Chloramphenicol served as a positive control, and distilled water as a negative control. The ointment formulation was tested at effective doses of 10% and 30% as per Pharmacopeia guidelines. The positive control used bactoderm ointment, and the negative control was an ointment base without clove extract. The results showed that clove ethanol extract could be formulated into an ointment after undergoing phytochemical screening. The clove ethanol extract ointment effectively inhibited bacterial growth causing skin infections. The inhibition zones for Streptococcus pyogenes were 12.16 mm and 14.06 mm, while for Pseudomonas aeruginosa they were 10.16 mm and 9.3 mm. The effective concentrations of clove ethanol extract ointment in inhibiting bacterial growth were 10% and 30%. Keywords: Bacteria, Skin Infection, Clove Ointment
Digitalizing Green Fashion Design via Service-Learning to Empower Fashion SMEs Rizki Amelia Nasution; M. Faisal Afiff Tarigan; Mohammed Aquil Mirza
Jurnal IPTEK Bagi Masyarakat Vol 5 No 2 (2025)
Publisher : Ali Institute of Research and Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55537/j-ibm.v5i2.1369

Abstract

This community service research introduces a model of digitalizing green fashion through a service-learning approach implemented at Rumah Jahit Nila, a fashion MSME in Medan. The intervention includes intensive training on 3D design software such as CLO 3D and Marvelous Designer, the use of AI-assisted tools like Repsketch for pattern optimization, and production mentoring throughout a 12-month project cycle. Measurements show an increase in students' average technical competency scores from 55.1 to 76.9, representing a gain of 21.8 points. Partner evaluation was conducted through satisfaction questionnaires and field observations, both of which indicated positive acceptance of digital patterns and user guides. Claims regarding waste reduction and material efficiency are currently indicative, as direct quantitative metrics such as percentage reduction in fabric use or paper pattern sheets are not yet available; thus, further measurement is recommended. This study offers a replicable digital technology-based community service model for empowering sustainable fashion MSMEs.
Co-Authors Almadina Septiyah Asti Hanipah Ita Hasanah Br Karo Kartika Manalu Linda Syafitri Siagian M. Faisal Afiff Tarigan Misriani Ani Mohammed Aquil Mirza Nur Ade Erma Nia Nurlayly Zaini Nurul Aina Harahap Rasyidah Riris Silvia Tampubolon S.Pd. M Kes I Ketut Sudiana . Suci Ramadani Pohan Suci Ramadhani Ulfayani Mayasari Vara Nabila Zahratul Idami Zulita Mega Anggriany