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All Journal BIOMA : Jurnal Biologi Makassar Martabe : Jurnal Pengabdian Kepada Masyarakat BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains KLOROFIL: Jurnal Ilmu Biologi dan Terapan Bioma : Jurnal Biologi dan Pembelajaran Biologi Best Journal (Biology Education, Sains and Technology) KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Rizki Amelia Nasution
Universitas Islam Negeri Sumatera Utara
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Public Health Social Sciences

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Uji Aktivitas Antimikroba Ekstrak Daun Randu (Ceiba pentandra L.) Terhadap Pertumbuhan Pseudomonas aeruginosa dan Candida albicans Misriani Ani; Kartika Manalu; Rizki Amelia Nasution
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.13740

Abstract

One of the traditional plants used by the community is the kapok plant (Ceiba pentandra L.) apart from being cultivated, parts of the kapok plant are also used as building materials, stuffing for mattresses and traditional medicine. The purpose of this study was to determine the activity of randu leaf extract on the growth of Pseudomonas aeruginosa and Candida albicans. This study used a completely randomized design (CRD) with 6 treatment groups, namely the positive control group with chloramphenicol and ketoconazole. The negative control group used 10% DMSO, and the extract concentration group was 70%, 80%, 90% and 100%. The steps carried out in this study included the extraction of kapok leaves using 96% ethanol as solvent, phytochemical screening and antimicrobial testing using the Kirby-Bauer Disc Diffusion method. Analysis of research data using SPSS 21. From the results of this study, it was found that randu leaf extract contained alkaloids, saponins and tannins. Antimicrobial testing with the kapok leaf extract group resulted in an average inhibition zone of 7.57 mm, 8.0 mm, 8.7 mm, and 9.87 mm against Pseudomonas aeruginosa and 4.67 mm, 5.07 mm, 5.32 mm, and 5.65 mm against Candida albicans. The results of the One Way ANOVA test show that Ftable < Fcount which proves that the leaf extract of Randu provides antimicrobial activity in inhibiting the growth of Pseudomonas aeruginosa and Candida albicans.
UJI AKTIVITAS MINYAK ATSIRI DAUN EKOR KUCING (Acalypha hispida) SEBAGAI DISINFEKTAN ALAMI TERHADAP BAKTERI Bacillus subtilis DAN Klebsiella pneumoniae SECARA IN- VITRO Nurlayly Zaini; Ulfayani Mayasari; Rizki Amelia Nasution
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 1 (2024): Bioma : Januari - Juni 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Bakteri Bacillus subtilis dan Klebsiella pneumoniae merupakan bakteri yang tersebar di udara, air dan tanah yang dapat menyebabkan penyakit infeksi meningitis. Penggunaan disinfektan alami minyak atsiri daun ekor kucing (Acalypha hispida) yang mengandung senyawa antibakteri flavonoid dan tanin akan menghambat pertumbuhan bakteri. Penelitian ini bertujuan untuk mengetahui aktivitas disinfektan alami minyak atsiri daun ekor kucing (Acalypha hispida) terhadap pertumbuhan bakteri Bacillus subtilis dan Klebsiella pneumoniae secara In-Vitro. Hasil uji aktivitas disinfektan alami minyak atsiri daun ekor kucing (Acalypha hispida) menggunakan konsentrasi 2,5%, 5%, 7,5%, dan 10%, diperoleh hasil diameter zona hambat pada bakteri Bacillus subtilis yaitu 17,54, 14,13, 13,66, dan 11,7 sedangkan hasil uji diameter zona hambat pada bakteri Klebsiella pneumonia yaitu 19,00, 16,57, 14,47, dan 12,59. Berdasarkan hasil dari rata-rata aktivitas antibakteri zona hambat minyak atsiri daun ekor kucing (Acalypha hispida) dapat dijadikan sebagai disinfektan alami terhadap bakteri Bacillus subtilis pada konsentrasi efektif 5%, sedangkan bakteri Klebsiella pneumoniae pada konsentrasi efektif 7,5%. Kata kunci: Minyak atsiri, disinfektan, Bacillus subtilis dan Klebsiella pneumoniae
Isolasi dan Identifikasi Bakteri Asam Laktat dari Hasil Fermentasi Limbah Organik Kulit Buah (Eco-Enzyme) Linda Syafitri Siagian; Kartika Manalu; Rizki Amelia Nasution
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 1 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i1.9967

Abstract

The aim of this research is the isolation and identification based on the morphological characteristics of lactic acid bacteria in the fermentation of organic waste from fruit peels. Isolation of Lactic Acid Bacteria was carried out using the spread plate method on de Man, Rogosa, and Sharpe Agar (MRSA) media. The culture was incubated at 37 °C for 48 hours. The growing colonies were observed for morphological characteristics, and bacterial Gram staining was carried out. The results of the research showed that 10 bacterial isolates were obtained from the isolation of BAL from ecoenzymes on de Man, Ragosa, and Sharpe Agar (MRSA) media, namely E-BAL 01, E-BAL 02, E-BAL O3, E-BAL 04, E-BAL 05, E-BAL 06, E-BAL 07, E-BAL 08, E-BAL 09, and E-BAL 10, and 10 isolates were characterized as Gram-positive with a bacillary cell form. In conclusion, 10 isolates were found with characteristics that supported MRSA media, namely isolates of lactic acid bacteria of the genus Lactobacillus. This is confirmed by the results of biochemical tests, namely that it is immobile, does not produce the catalase enzyme, and does not have the ability to produce gas. Keywords: Lactic Acid Bacteria (LAB), Ecoenzymes, Fruit Skin
Isolasi dan Identifikasi Bakteri Endofit Penghasil Hormon IAA (Indole Acetic Acid) dari Akar Tanaman Mangrove Avicennia marina Suci Ramadani Pohan; Kartika Manalu; Rizki Amelia Nasution
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 1 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i1.9968

Abstract

This research aims to determine the genus and potential isolates of endophytic bacteria producing IAA hormone from the roots of mangrove plants in Tanjung Rejo village, Percut Sai Tuan subdistrict. The research methods used include isolating endophytic bacteria using the plant method in NA (Nutrient Agar) media then culturing them in NB (Nutrient Broth) media then adding L-tryptophan and incubating, then identification is carried out based on morphological characterization, gram staining and biochemistry. The results of the research showed that 13 isolates of endophytic bacteria were found that had the potential to produce IAA hormones ranging from 0.047-0.050 ppm which were identified as the genus Enterobacter, Pseudomonas, Bacillus, Azosprilium, Micrococcus. However, the isolates BEAM PA 2 of the genus Enterobacter and BPAM PE 6 of the genus Micrococcus had the highest value of 0.050 ppm. The conclusion from this research is that the endophytic bacteria obtained as a whole have the ability to produce the hormone IAA. Keywords: Endophytic Bacteria, IAA (Indole Acetic Acid), Mangrove Avicennia marina
Co-Authors Almadina Septiyah Asti Hanipah Kartika Manalu Linda Syafitri Siagian Misriani Ani Nur Ade Erma Nia Nurlayly Zaini Riris Silvia Tampubolon S.Pd. M Kes I Ketut Sudiana . Suci Ramadani Pohan Suci Ramadhani Ulfayani Mayasari Zahratul Idami Zulita Mega Anggriany