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All Journal BIOMA : Jurnal Biologi Makassar Journal of Development Research BIOTROPIC The Journal of Tropical Biology BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains KLOROFIL: Jurnal Ilmu Biologi dan Terapan Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Best Journal (Biology Education, Sains and Technology) BINOMIAL : Jurnal Pendidikan Biologi KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Abdi Mas Adzkia International Conference on Sciences Development and Technology
Ulfayani Mayasari
Universitas Islam Negeri Sumatera Utara
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KARAKTERISASI SIMPLISIA DAN SKRINING FITOKIMIA DAUN JERUK LEMON (Citrus limon (L.) Burm.f.) Ulfayani Mayasari; Melfin Teokarsa Laoli
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 2, No 1 (2018): Klorofil: Jurnal Ilmu Biologi dan Terapan
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v2i1.1802

Abstract

Lemon Citrus (Citrus limon (L.) Burm f.) is one of the plants that has potential as a traditional medicine, and is an excellent source of vitamin C and calcium. Lemon leaf lemon can help overcome cancer, heart and liver. The purpose of this research is to characterize simplicia leaf and to know the chemical compound group contained in leaf. Characterization of simplicia include macroscopic and microscopic examination, determinations of loss on drying, determinations of water soluble extract, determination of ethanol soluble concentration, determination of total ash content, and determination of acid ash in soluble. Phytochemical screening includes the test for alkaloids, flavonoids, steroids/triterpenoids, tannins and saponins. The result obtained from examination of lemon citrus leaf characteristic gave loss on drying 69,29%; total ash content 6,40%; acid insoluble ash 1,23%; ethanol soluble extract 17,73%; water soluble extract 24,79%. The result of phytochemical screening showed that lemon leaf simplicia extract contains alkaloids, flavonoids, steroids/triterpenoids and tannins.
UJI DAYA HAMBAT ANTIJAMUR BUAH BELIMBING WULUH (Averrhoa bilimbi L.) TERHADAP PERTUMBUHAN Pityrosporum ovale PENYEBAB KETOMBE Marliana Marliana; Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 4, No 2 (2020): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v4i2.8976

Abstract

One of the plants that can be used as medicine is Wuluh starfruit (Averrhoa bilimbi L.). Starfruit fruit has antipyretic, antiseptic, anti-inflammatory properties, treats coughs and treats rashes. Wuluh starfruit (Averrhoa bilimbi L.) contains chemical compounds of flavonoids, saponins, alkaloids, and tannins. This study aims to determine the anti-fungal inhibition of star fruit (Averrhoa bilimbi L.) on the growth of Pityrosporum ovale which causes dandruff. The method used to obtain the extract of starfruit (Averrhoa bilimbi L.) through a maceration process using ethanol 96% solvent. The anti-fungal inhibition test used the agar diffusion method by sticking disc paper on the media that had been grown by the fungus Pityrosporum ovale. The results of phytochemical screening showed that the fruit of starfruit (Averrhoa bilimbi L.) contained flavonoid, alkaloid, tannin and saponin compounds. The resulting inhibitory power was indicated by the formation of a clear zone at each concentration of 20%, 40%, 60%, 80%, and as a comparison, a positive control of 10% ketonazole was made. The average diameter obtained was 6.26 mm, 7.09 mm, 7.57 mm, 8.07 mm, and 17.29 mm. Based on the results of the study, it was concluded that the extract of starfruit (Averrhoa bilimbi L.) had an inhibitory power against the growth of Pityrosporum ovale.
KARAKTERISASI SIMPLISIA DAN SKRINING FITOKIMIA DAUN JERUK LEMON (Citrus limon (L.) Burm.f.) Ulfayani Mayasari; Melfin Teokarsa Laoli
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 2, No 1 (2018): Klorofil: Jurnal Ilmu Biologi dan Terapan
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v2i1.1802

Abstract

Lemon Citrus (Citrus limon (L.) Burm f.) is one of the plants that has potential as a traditional medicine, and is an excellent source of vitamin C and calcium. Lemon leaf lemon can help overcome cancer, heart and liver. The purpose of this research is to characterize simplicia leaf and to know the chemical compound group contained in leaf. Characterization of simplicia include macroscopic and microscopic examination, determinations of loss on drying, determinations of water soluble extract, determination of ethanol soluble concentration, determination of total ash content, and determination of acid ash in soluble. Phytochemical screening includes the test for alkaloids, flavonoids, steroids/triterpenoids, tannins and saponins. The result obtained from examination of lemon citrus leaf characteristic gave loss on drying 69,29%; total ash content 6,40%; acid insoluble ash 1,23%; ethanol soluble extract 17,73%; water soluble extract 24,79%. The result of phytochemical screening showed that lemon leaf simplicia extract contains alkaloids, flavonoids, steroids/triterpenoids and tannins.
UJI DAYA HAMBAT ANTIJAMUR BUAH BELIMBING WULUH (Averrhoa bilimbi L.) TERHADAP PERTUMBUHAN Pityrosporum ovale PENYEBAB KETOMBE Marliana Marliana; Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 4, No 2 (2020): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v4i2.8976

Abstract

One of the plants that can be used as medicine is Wuluh starfruit (Averrhoa bilimbi L.). Starfruit fruit has antipyretic, antiseptic, anti-inflammatory properties, treats coughs and treats rashes. Wuluh starfruit (Averrhoa bilimbi L.) contains chemical compounds of flavonoids, saponins, alkaloids, and tannins. This study aims to determine the anti-fungal inhibition of star fruit (Averrhoa bilimbi L.) on the growth of Pityrosporum ovale which causes dandruff. The method used to obtain the extract of starfruit (Averrhoa bilimbi L.) through a maceration process using ethanol 96% solvent. The anti-fungal inhibition test used the agar diffusion method by sticking disc paper on the media that had been grown by the fungus Pityrosporum ovale. The results of phytochemical screening showed that the fruit of starfruit (Averrhoa bilimbi L.) contained flavonoid, alkaloid, tannin and saponin compounds. The resulting inhibitory power was indicated by the formation of a clear zone at each concentration of 20%, 40%, 60%, 80%, and as a comparison, a positive control of 10% ketonazole was made. The average diameter obtained was 6.26 mm, 7.09 mm, 7.57 mm, 8.07 mm, and 17.29 mm. Based on the results of the study, it was concluded that the extract of starfruit (Averrhoa bilimbi L.) had an inhibitory power against the growth of Pityrosporum ovale.
Uji Aktivitas Antibakteri ekstrak Batang Muda Rotan Manau (Calamus manan) terhadap pertumbuhan bakteri Klebsiella pneumonia Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 1 (2022): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i1.11762

Abstract

Batang muda rotan manau dapat diolah menjadi makanan seperti makanan khas Mandailing Natal di Tapanuli Selatan Sumatera Utara yang biasa disebut dengan pakkat. Selain sebagai bahan makanan, batang muda rotan manau juga  sebagai bahan obat-obatan karna mengandung senyawa fitokimia yang mampu menjadi antibakteri. Salah satu penyakit yanhg disebabkan bakteri yaitu pneumonia yang disebabkan bakteri Klebsiella pneumonia. Tujuan penelitian ini adalah untuk mengetahui kemampuan batang muda rotan manau dalam menghambat pertumbuhan bakteri Klebsiella pneumonia. Metode yang dilakukan dalam penelitian ini adalah dengan pengujian skrining fitokimia untuk mengetahui kandungan metabolit sekunder batang muda rotan manau dan selanjutnya pengujian aktivitas antibakteri dengan metode difusi cakram dengan konsentrasi ekstrak 20%,40%, 60%, 80%. Hasil skrinig fitokimia menunjukkan batang muda rotan manau mengandung flavonoid, glikosida, saponin dan tanin. Pada uji aktivitas antibakteri diperoleh zona hambat pada masing-masing konsentrasi dari yang terendah yaitu 11,38mm , 12,30mm, 13,78mm, 14,33mm. Disimpulkan bahwa batang muda rotan manau mampu menghambat pertumbuhan bakteri Klebsiella pneumonia. Keywords: antibakteri, Rotan manau (Calamus manan), Klebsiella pneumonia
Media Alternatif Pertumbuhan Bakteri dari Daging Buah Kelapa (Cocos nucifera) Terhadap Bakteri Escherichia Coli dan Staphylococcus aureus Thoibah Sinaga; Kartika Manalu; Ulfayani Mayasari
BEST Journal (Biology Education, Sains and Technology) Vol 5, No 1 (2022): Juni 2022
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v5i1.5207

Abstract

Tujuan dari penelitian ini untuk mengetahui pemanfaatan daging buah kelapa (Cocos nucifera) sebagai media alternatif pertumbuhan bakteri. Penelitian ini menggunakan metode eksperimen dimana variabel bebas adalah konsentrasi tepung daging buah kelapa dengan variasi 16%, 18%, 20%, 22% dan 24% yang masing-masing konsentrasi diulang sebanyak lima kali. Hasil yang diperoleh jumlah populasi koloni bakteri Escherichia coli pada tepung daging buah kelapa konsentrasi 16% sebanyak 137 x 106, 18% sebanyak 119 x 106, 20% sebanyak 74 x 106, 22% sebanyak 93 x 106  dan 24% sebanyak 74 x 106. Jumlah populasi koloni bakteri Staphylococcus aureus pada tepung daging buah kelapa konsentrasi 16% sebanyak 187 x 106, 18% sebanyak 20 x 106, 20% sebanyak 65 x 106, 22% sebanyak 23 x 106 dan 24% sebanyak 34 x 106. Pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus yang paling baik adalah pada media daging buah kelapa konsentrasi 16%. Hasil data dianalisis menggunakan SPSS 25 dengan metode uji one way ANOVA. Hasil data dianalisis menggunakan SPSS 25 dengan metode uji one way ANOVA. Hasil analisis statistik menunjukkan tidak ada perbedaan yang bermakna pada setiap kelompok perlakuan ( p0,05) dan didapat Fhitung Ftabel menyatakan bahwa tidak ada pengaruh nyata antara variasi konsentrasi terhadap pertumbuhan Escherichia coli, sedangkan hasil analisis statistik pada pertumbuhan bakteri Staphylococcus aureus menunjukkan ada perbedaan yang bermakna pada setiap kelompok perlakuan ( p  0,05) dan didapat Fhitung Ftabel menyatakan bahwa ada pengaruh nyata antara variasi konsentrasi terhadap pertumbuhan bakteri Staphylococcus aureus.
Skrining Bakteri yang Bersimbiosis dengan Spons dari Pulau Bakar dalam Menghambat Pertumbuhan Bakteri Escherichia coli Ulfayani Mayasari; Rizki Amelia Nasution
BEST Journal (Biology Education, Sains and Technology) Vol 5, No 1 (2022): Juni 2022
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v5i1.5319

Abstract

 Simbiosis mutualisme spons dengan bakteri mampu menghasilkan senyawa bioaktif salah satunya ialah sebagai antibakteri. Tujuan dari penelitian ini adalah mengisolasi dan melakukan pencarian bakteri yang bersimbiosis dengan spons di Pulau Bakar yang mempunyai aktivitas antibakteri terhadap Escherichia coli. Tahapan penelitian ini yaitu isolasi dan identifikasi bakteri simbions spons selanjutnya pengujian aktivitas antibakteri dengan metode difusi cakram. Pada hasil penelitian ini ditemukan 16 jenis bakteri yang bersimbiosis dengan spons. Isolat terbaik yang mampu menghambat bakteri Escherichia coli ialah SPS4(2)B dengan diameter zona hambat yang ditimbulkan ialah  12,01 mm dan tergolong ke dalam kategori kuat. Dapat disimpulkan bahwa bakteri yang bersimbiosis dengan spons di perairan Pulau Bakaran mampu menghambat pertumbuhan bakteri Escherichia coli Kata Kunci: antibakteri, bakteri bersimbiosis,  spons, Escherichia coli
Uji Aktivitas Antibakteri ekstrak Batang Muda Rotan Manau (Calamus manan) terhadap pertumbuhan bakteri Klebsiella pneumonia Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 1 (2022): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i1.11762

Abstract

Batang muda rotan manau dapat diolah menjadi makanan seperti makanan khas Mandailing Natal di Tapanuli Selatan Sumatera Utara yang biasa disebut dengan pakkat. Selain sebagai bahan makanan, batang muda rotan manau juga  sebagai bahan obat-obatan karna mengandung senyawa fitokimia yang mampu menjadi antibakteri. Salah satu penyakit yanhg disebabkan bakteri yaitu pneumonia yang disebabkan bakteri Klebsiella pneumonia. Tujuan penelitian ini adalah untuk mengetahui kemampuan batang muda rotan manau dalam menghambat pertumbuhan bakteri Klebsiella pneumonia. Metode yang dilakukan dalam penelitian ini adalah dengan pengujian skrining fitokimia untuk mengetahui kandungan metabolit sekunder batang muda rotan manau dan selanjutnya pengujian aktivitas antibakteri dengan metode difusi cakram dengan konsentrasi ekstrak 20%,40%, 60%, 80%. Hasil skrinig fitokimia menunjukkan batang muda rotan manau mengandung flavonoid, glikosida, saponin dan tanin. Pada uji aktivitas antibakteri diperoleh zona hambat pada masing-masing konsentrasi dari yang terendah yaitu 11,38mm , 12,30mm, 13,78mm, 14,33mm. Disimpulkan bahwa batang muda rotan manau mampu menghambat pertumbuhan bakteri Klebsiella pneumonia. Keywords: antibakteri, Rotan manau (Calamus manan), Klebsiella pneumonia
UJI POTENSI BAKTERI ENDOFIT DARI DAGING BUAH SALAK PONDOH (Salacca edulis) DAN SALAK PADANGSIDEMPUAN (Salacca sumatrana) DALAM MENGHAMBAT PERTUMBUHAN Escherichia coli Rizki Amelia Nasution; Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.12354

Abstract

Salak is a fruit that contains various health benefits, including the flesh of the fruit which contains bioactive compounds. Taking bioactive compounds directly from the flesh of the salak fruit will require quite a lot of biomass. Obtaining these bioactive compounds can be streamlined by using endophytic bacteria. Endophytic bacteria able to produce compounds of  antibacterial. The aimed of this study to examine the antibacterial potential of  endophytic bacteria derived from the flesh of the Pondoh and Padangsidempuan salak fruit against pathogenic bacteria, namely Escherichia coli. Method this studies is a laboratory experiment. A total of 10 isolates consisting of 6 isolates of Pondoh salak and 4 isolates of Padangsidempuan salak used were the results of isolation from salak fruit flesh in previous studies. Based on the results of the study, ten isolates indicate of antimicrobial activity to Escherichia coli. The inhibition zone diameter of highest value was owned by SP4 isolate, which was 1.1 mm. These results indicate that endophytic bacteria from the pulp of Pondoh and Padangsidempuan salak possess the potential to produce antibacterial compounds against gram-negative pathogenic bacteria, namely Escherichia coli. 
UJI POTENSI BAKTERI ENDOFIT DARI DAGING BUAH SALAK PONDOH (Salacca edulis) DAN SALAK PADANGSIDEMPUAN (Salacca sumatrana) DALAM MENGHAMBAT PERTUMBUHAN Escherichia coli Rizki Amelia Nasution; Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.12354

Abstract

Salak is a fruit that contains various health benefits, including the flesh of the fruit which contains bioactive compounds. Taking bioactive compounds directly from the flesh of the salak fruit will require quite a lot of biomass. Obtaining these bioactive compounds can be streamlined by using endophytic bacteria. Endophytic bacteria able to produce compounds of  antibacterial. The aimed of this study to examine the antibacterial potential of  endophytic bacteria derived from the flesh of the Pondoh and Padangsidempuan salak fruit against pathogenic bacteria, namely Escherichia coli. Method this studies is a laboratory experiment. A total of 10 isolates consisting of 6 isolates of Pondoh salak and 4 isolates of Padangsidempuan salak used were the results of isolation from salak fruit flesh in previous studies. Based on the results of the study, ten isolates indicate of antimicrobial activity to Escherichia coli. The inhibition zone diameter of highest value was owned by SP4 isolate, which was 1.1 mm. These results indicate that endophytic bacteria from the pulp of Pondoh and Padangsidempuan salak possess the potential to produce antibacterial compounds against gram-negative pathogenic bacteria, namely Escherichia coli. 
Co-Authors Asfianti, Vivi Diva Mawaddah Febiyaula, Siti Septia Hafizah, Casyariadi Vana Intan Fatimah Azzahra intan Kartika Manalu Mandalika, Mandalika Marbun, Eva Diansari Marliana Marliana Melfin Teokarsa Laoli muhammad fadhil Muhammad Haris Nasution, Fadya Safira Nasution, Rizki Amelia Novita Fanny Nurlayly Zaini Raissa Amanda Putri Rasyidah Revina Yani Tarigan Revina Yani Tarigan Rizki Amelia Nasution Rizki Amelia Nasution Rizki Amelia Nasution Sapitri, Alfi Sapitri, Alfi Sintia Ariska Putri Siti Halimah Syahputri, Siti Novi Astri Thoibah Sinaga Vila Tria Putri Yardha, La Saufa Zulia Wirdani Putri Nasution