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All Journal Best Journal (Biology Education, Sains and Technology) Food ScienTech Journal
Muhammad Badrut Tamam
Universitas Muhammadiyah Lamongan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Education Immunology & microbiology Public Health

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Banana (Musa × Paradisiaca) Corm as an Alternative Substrate for Nata by Acetobacter xylinum M. Ainul Mahbubillah; Fatma Atiq Khoridah; Muhammad Badrut Tamam; Fatan Mujahid
Food ScienTech Journal Vol 7, No 2 (2025)
Publisher : University of Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/fsj.v7i2.33847

Abstract

Banana corms, an underutilized by-product of banana (Musa × paradisiaca) cultivation, were investigated as a novel substrate for nata production using Acetobacter xylinum fermentation. This study evaluated the effects of varying sucrose concentrations (5%, 10%, and 15%), ammonium sulfate levels (0.5%, 1%, and 1.5%), and pH (3.5, 4, and 4.5) on nata thickness, wet weight, dry weight, water holding capacity (WHC), and organoleptic properties. The highest nata thickness (6.44 mm) and wet weight were observed in S3, A3, P3 (15% sucrose, 1.5% ammonium sulfate, pH 4.5). The highest dry weight was also obtained under these conditions. WHC varied between 6.50% and 17.00%, with S3, A2, P2 (15.00%) and S2, A3, P1 (16.00%) showing the highest values. Organoleptic analysis indicated that banana corm nata had a murky colour, slightly chewy texture, and sour taste, comparable to nata de coco but differing in acidity. The findings confirm that banana corms are a viable substrate for nata production, providing an innovative and sustainable approach to agricultural waste utilization with potential industrial applications.
Diesel Fuel Degradation Activity of Hydrocarbon-Degrading Bacteria from Nusantara Fisheries Port Brondong Lamongan M. Ainul Mahbubillah; Muhammad Badrut Tamam; Rieke Dwi Ayuni; Nynda Ayu Nadira Savitri; Aisyah Hadi Ramadani
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.10887

Abstract

The Nusantara Fisheries Port (Pelabuhan Perikanan Nusantara - PPN) in Brondong, Lamongan Regency, is a port with high ship activities. This has led to hydrocarbon pollution in the form of diesel fuel and oil in the waters of PPN Brondong. The initial effort to address this pollution is by isolating and characterizing hydrocarbon-degrading bacteria present in these waters. This research is a strategic step in managing petroleum hydrocarbon spills in the area through bioremediation processes, either via bioaugmentation or biostimulation for further study. The research method involves isolating hydrocarbon-degrading bacteria from the waters of PPN Brondong using the spread plate isolation method. Different colony characteristics are selected for the purification of bacterial isolates. The obtained bacterial isolates are then subjected to hydrocarbon degradation activity by culturing them in a minimal medium containing diesel fuel. The degradation activity is measured using total petroleum hydrocarbon (TPH) testing. The results of this study yielded 7 different isolates from the fuel oil-contaminated sites in PPN Brondong. Culturing in a minimal medium containing diesel fuel yielded results showing that all isolates possess the ability to degrade hydrocarbons, as evidenced by the reduction in TPH.
Co-Authors Aisyah Hadi Ramadani Fatan Mujahid Fatma Atiq Khoridah Mahbubillah, M. Ainul Nynda Ayu Nadira Savitri Rieke Dwi Ayuni