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All Journal Best Journal (Biology Education, Sains and Technology) Livestock and Animal Research
Muhammad Badrut Tamam
Universitas Muhammadiyah Lamongan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Public Health Veterinary

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Deteksi coronavirus pada kelelawar di Kabupaten Lamongan Muhammad Badrut Tamam; Aisyah Hadi Ramadani; M. Ainul Mahbubillah
Livestock and Animal Research Vol 20, No 1 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1027.802 KB) | DOI: 10.20961/lar.v20i1.56667

Abstract

Objective: To obtain information about the presence of coronavirus in bats to find potential of new disease reservoir as well as not yet reported disease reservoir in Lamongan District. This research can be used by the government as a basis for planning and evaluating disease control programs and for researchers it can be used for vaccine and drug design, viral phylogenetic, analysis of viral distribution, and viral databases.Methods: Sampling was carried out at 3 points representing different habitat types. The first point is at Pucakwangi Cave Babat Lamongan, the second is at Paciran Lamongan beach area, and the last is at the Mantup Forest Lamongan. Sampling was carried out using a mist net with the help of local workers. The netted bats were caught and put in a bag. Parameters observed included gender, morphometry, species, and detection of the presence of coronavirus. Detection of the virus using the oropharyngeal swab method, then the RNA samples were tested by RT-PCR and sequencing. The primer used to detect the coronavirus is primer forward 5′-CACGCAACTTGTTGTAATGCGT CAGAGA-3′ and primer reverse 5′- CACGTGCTTTTGCAGGCACTATACGAC-3′.Results: Five species of bats obtained from 3 sampling locations namely Taphozous melanopogon, Chaerophon sp., Hipposideros sp., Cynopterus sp., Macroglossus sp. RT-PCR test of sample G1, G6, G8, P1, P2, P3, H1, H3, and H6, shows that no coronavirus from DNA sample amplified.Conclusions: Negative result of Coronavirus detection by molecular analysis is not an indicator of no potential of species Taphozous melanopogon, Chaerophon sp., Hipposideros sp., Cynopterus sp., and Macroglossus sp. as a Coronavirus reservoir. Increasing the number of samples and expansion of study area still needed to obtain more comprehensive data.
Diesel Fuel Degradation Activity of Hydrocarbon-Degrading Bacteria from Nusantara Fisheries Port Brondong Lamongan M. Ainul Mahbubillah; Muhammad Badrut Tamam; Rieke Dwi Ayuni; Nynda Ayu Nadira Savitri; Aisyah Hadi Ramadani
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.10887

Abstract

The Nusantara Fisheries Port (Pelabuhan Perikanan Nusantara - PPN) in Brondong, Lamongan Regency, is a port with high ship activities. This has led to hydrocarbon pollution in the form of diesel fuel and oil in the waters of PPN Brondong. The initial effort to address this pollution is by isolating and characterizing hydrocarbon-degrading bacteria present in these waters. This research is a strategic step in managing petroleum hydrocarbon spills in the area through bioremediation processes, either via bioaugmentation or biostimulation for further study. The research method involves isolating hydrocarbon-degrading bacteria from the waters of PPN Brondong using the spread plate isolation method. Different colony characteristics are selected for the purification of bacterial isolates. The obtained bacterial isolates are then subjected to hydrocarbon degradation activity by culturing them in a minimal medium containing diesel fuel. The degradation activity is measured using total petroleum hydrocarbon (TPH) testing. The results of this study yielded 7 different isolates from the fuel oil-contaminated sites in PPN Brondong. Culturing in a minimal medium containing diesel fuel yielded results showing that all isolates possess the ability to degrade hydrocarbons, as evidenced by the reduction in TPH.
Co-Authors Aisyah Hadi Ramadani Mahbubillah, M. Ainul Nynda Ayu Nadira Savitri Rieke Dwi Ayuni