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All Journal AL KAUNIYAH Techne : Jurnal Ilmiah Elektroteknika Best Journal (Biology Education, Sains and Technology) PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Livestock and Animal Research Genbinesia Journal of Biology
Mahbubillah, M. Ainul
Universitas Muhammadiyah Lamongan
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Uji Aktivitas Antibakteri pada Ekstrak Etanolik Teripang Kering dari Ujung Pangkah Gresik M. Ainul Mahbubillah; Putri Ayu Ika Setiyowati; Muhammad Badrut Tamam; Fika Nur Fitriana Putri Bukhori
BEST Journal (Biology Education, Sains and Technology) Vol 5, No 1 (2022): Juni 2022
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v5i1.5233

Abstract

Sea cucumber is a potential fishery sub-sector, but its use still not popular because of its unfavorable physical form. However, from its unfavorable form, sea cucumbers contain many benefits as nutrition and medicine, including the presence of antimicrobial activity. The use of sea cucumbers as a nutrient is used by the people of Ujung Pangkah Gresik village as crackers. This study aims to prove the antimicrobial potential of dried sea cucumbers with crackers from Ujung Pangkah village, Gresik. The sea cucumbers have been boiled first before drying in the sun. The dried sea cucumbers were blended and then extracted using 96% ethanol. The extract was then sterilized by filtration using a 0.2 m PTFE filter. Extracts were tested using the Kirby-Bauer method on Mueller-Hinton Agar medium inoculated with Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Burkholderia cepacia, and yeast Candida albicans. The control antibiotic tetracycline 30 µg/ml and antifungal 2,5 µg/ml fluconazole were used as comparisons. The results of this study showed that all ethanolic extracts of dry sea cucumber with concentrations of 5%, 10%, and 15% did not show any clear zone. While the clear zone was shown in the control of tetracycline and fluconazole. This was caused by the loss of antimicrobial activity in the ethanolic extract of dried sea cucumbers derived from antimicrobial peptides which were damaged by heating during boiling process.
Inventarisasi Bryophyta, Pteridophyta, Gymnospermae Di Kabupaten Lamongan Dwi Zulianti; M. Ainul Mahbubillah; Nynda Ayu Nadira Savitri; Rofiatun Solekha
BEST Journal (Biology Education, Sains and Technology) Vol 4, No 1 (2021): Juni 2021
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v4i1.3596

Abstract

Indonesia has rich of plant species because of its habitat variation. Bryophyta, Pteridophyta, and Gymnospermae mostly grow on highland habitat. Height of the habitat causes change of micro-climate, especially humidity. Lamongan District of East Java Indonesia Jawa Timur is a lowland area so that difficult to find species of those three taxa. For educational purposes, data collection of the 3 taxa species that grow in Lamongan District is needed. The research was carried out by identifying plants Bryophyta, Pteridophyta, and in Lamongan District. The results of the study found that there were 3 Bryophyte species: Marchantia polymorpha, Polytrichum sp, and Riccia sp.; 3 Pteridophyte species: Pteridium aquilinum, Adiantum philippens, and Telmatoblechnum indicum; and 2 Gymnosperm species: Cycas rumphii, and Gnetum gnemon.   
Deteksi coronavirus pada kelelawar di Kabupaten Lamongan Muhammad Badrut Tamam; Aisyah Hadi Ramadani; M. Ainul Mahbubillah
Livestock and Animal Research Vol 20, No 1 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1027.802 KB) | DOI: 10.20961/lar.v20i1.56667

Abstract

Objective: To obtain information about the presence of coronavirus in bats to find potential of new disease reservoir as well as not yet reported disease reservoir in Lamongan District. This research can be used by the government as a basis for planning and evaluating disease control programs and for researchers it can be used for vaccine and drug design, viral phylogenetic, analysis of viral distribution, and viral databases.Methods: Sampling was carried out at 3 points representing different habitat types. The first point is at Pucakwangi Cave Babat Lamongan, the second is at Paciran Lamongan beach area, and the last is at the Mantup Forest Lamongan. Sampling was carried out using a mist net with the help of local workers. The netted bats were caught and put in a bag. Parameters observed included gender, morphometry, species, and detection of the presence of coronavirus. Detection of the virus using the oropharyngeal swab method, then the RNA samples were tested by RT-PCR and sequencing. The primer used to detect the coronavirus is primer forward 5′-CACGCAACTTGTTGTAATGCGT CAGAGA-3′ and primer reverse 5′- CACGTGCTTTTGCAGGCACTATACGAC-3′.Results: Five species of bats obtained from 3 sampling locations namely Taphozous melanopogon, Chaerophon sp., Hipposideros sp., Cynopterus sp., Macroglossus sp. RT-PCR test of sample G1, G6, G8, P1, P2, P3, H1, H3, and H6, shows that no coronavirus from DNA sample amplified.Conclusions: Negative result of Coronavirus detection by molecular analysis is not an indicator of no potential of species Taphozous melanopogon, Chaerophon sp., Hipposideros sp., Cynopterus sp., and Macroglossus sp. as a Coronavirus reservoir. Increasing the number of samples and expansion of study area still needed to obtain more comprehensive data.
The Quantification of Lead Heavy Metals Levels on Mujair Fish (Oreochromis mossambicus) Organs From Brantas and Bengawan Solo River, East Java Province, Indonesia Putri Ayu Ika Setiyowati; Aisyah Hadi Ramadani; Suhariyati Suhariyati; M Ainul Mahbubillah
Al-Kauniyah: Jurnal Biologi Vol 17, No 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

AbstractOreochromis mossambicus (O. mossambicus) frequently found in the Brantas and Bengawan Solo rivers, Java island, Indonesia. However, heavy metals produced from anthropogenic activities can enter the water and accumulate in organisms living in the river. This study aimed to determine the lead (Pb) heavy metal in the gills, flesh, and intestines of O. mossambicus living in the two aforementioned rivers and to measure the Pb levels in each river. The results showed that the Pb in the O. mossambicus organs in the Bengawan Solo river was as follows 3.159 mg/kg in the gills; 1.930 mg/kg in the intestine; and 2.511 mg/kg in flesh, while in the Brantas river it was follows 1.600 mg/kg in gills; 1.402 mg/kg in the intestine; and 1.455 mg/kg flesh. Pb levels in each river water were 0.568 mg/mL in the Brantas river and 0.525 mg/mL in the Bengawan Solo river. Based on the data obtained, it can be concluded that the Pb content in fish organs and river water has exceeded the quality standard for Pb levels according to the government regulation No.82 2001 (SNI 7387:2009), that is, 0.3 mg/kg in organs and 0.03 mg/L in water. The results of this study are expected to be a concern for the authorities in order to revitalize the river to restore the function and support the survival of river biota.AbstrakIkan mujair (Oreochromis mossambicus) banyak ditemukan di sungai Brantas dan Bengawan Solo, namun aktivitas antropogenik yang menghasilkan logam berat dapat masuk ke perairan sehingga terakumulasi dalam organisme yang hidup di perairan tersebut. Penelitian ini bertujuan untuk mengetahui kandungan logam berat timbal (Pb) pada insang, daging, dan usus pada O. Mossambicus yang hidup di kedua sungai tersebut serta mengukur kandungan Pb pada masing-masing air sungai. Hasil penelitian menunjukkan bahwa kandungan Pb pada organ O. mossambicus di sungai Bengawan Solo adalah sebagai berikut 3.159 mg/kg pada insang; 1.930 mg/kg di usus; dan 2.511 mg/kg pada daging, sedangkan di sungai Brantas adalah sebagai berikut 1.600 mg/kg pada insang; 1,402 mg/kg pada usus; dan 1,455 mg/kg pada daging. Kadar Pb pada masing-masing air sungai adalah 0,568 mg/mL (sungai Brantas) dan 0,525 mg/mL (sungai Bengawan Solo). Berdasarkan data yang diperoleh dapat disimpulkan bahwa kandungan Pb pada organ ikan maupun air sungai sudah melebihi baku mutu kadar Pb pada organ yaitu 0,3 mg/kg (SNI 7387:2009) dan 0,03 mg/L pada perairan (PP No.82 tahun 2001). Hasil penelitian ini diharapkan dapat menjadi perhatian pihak-pihak terkait agar dapat melakukan revitalisasi sungai guna mengembalikan fungsi dan mendukung keberlangsungan hidup biota sungai.
Diesel Fuel Degradation Activity of Hydrocarbon-Degrading Bacteria from Nusantara Fisheries Port Brondong Lamongan Mahbubillah, M. Ainul; Tamam, Muhammad Badrut; Ayuni, Rieke Dwi; Savitri, Nynda Ayu Nadira; Ramadani, Aisyah Hadi
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.10887

Abstract

The Nusantara Fisheries Port (Pelabuhan Perikanan Nusantara - PPN) in Brondong, Lamongan Regency, is a port with high ship activities. This has led to hydrocarbon pollution in the form of diesel fuel and oil in the waters of PPN Brondong. The initial effort to address this pollution is by isolating and characterizing hydrocarbon-degrading bacteria present in these waters. This research is a strategic step in managing petroleum hydrocarbon spills in the area through bioremediation processes, either via bioaugmentation or biostimulation for further study. The research method involves isolating hydrocarbon-degrading bacteria from the waters of PPN Brondong using the spread plate isolation method. Different colony characteristics are selected for the purification of bacterial isolates. The obtained bacterial isolates are then subjected to hydrocarbon degradation activity by culturing them in a minimal medium containing diesel fuel. The degradation activity is measured using total petroleum hydrocarbon (TPH) testing. The results of this study yielded 7 different isolates from the fuel oil-contaminated sites in PPN Brondong. Culturing in a minimal medium containing diesel fuel yielded results showing that all isolates possess the ability to degrade hydrocarbons, as evidenced by the reduction in TPH.
BUILDING THE CAPACITY OF ORGANIC FARMERS IN PRIJEKNGABLAK VILLAGE FOR INDEPENDENT PRODUCTION OF BIOPESTICIDES AND LIQUID ORGANIC FERTILIZERS Mahbubillah, M. Ainul; Redjeki, Endah Sri; Khakim, Arsanul; Ayuni, Rieke Dwi; Rohmah, Laila Ainur
PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Vol 4 No 1 (2024): Desember
Publisher : Fakultas Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/pakdemas.v4i1.329

Abstract

In 2012, Prijekngablak Village, Karanggeneng, Lamongan, faced crop failure due to an uncontrollable pest outbreak. This prompted the Rame Gawe II Farmers' Group to initiate organic farming, despite various social challenges. In 2022, the village government supported these efforts, enabling the community to produce liquid organic fertilizer (POC) and bio-pesticides. However, obstacles such as low public trust, poor institutional coordination, and inadequate laboratory facilities persisted. The Community Service team addressed these issues through focus group discussions (FGDs), facility improvements, laboratory management enhancements, and training on producing and applying POC and bio-pesticides. FGDs revealed that 54.3% of farmers had a strong understanding of organic farming, 62.9% moderately understood its benefits, 51.4% had practical experience, and 77.1% were willing to adopt it. Laboratory improvements and SOP implementation improved production performance. Key products included POC from water hyacinths, bio-pesticides containing Beauveria bassiana and Trichoderma sp., and PGPR isolated from local bamboo roots. Their application enhanced plant health and reduced pest and disease attacks, showcasing the potential of organic farming in overcoming agricultural challenges in the village.
Characterization of Rhizosphere Bacteria in the Rice Fields of the Sawah Tambak Rice-Fish Farming System Mahbubillah, M. Ainul; Aisyah, Neelam; Solekha, Rofiatun; Rohmah, Laila Ainur
BEST Journal (Biology Education, Sains and Technology) Vol 7, No 1 (2024): Juni 2024
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v7i1.10915

Abstract

The Sawah Tambak Rice-Fish Farming System is a land-use practice that alternates between rice cultivation and fish farming depending on the season. During the dry season, the land is used for growing rice, while in the rainy season, it functions as a fishpond. Successful rice cultivation in this system relies on beneficial rhizosphere bacteria that interact with plant roots through biological, physical, and chemical processes. This study aims to characterize bacteria isolated from the rhizosphere of rice plants (Oryza sativa L.) in this system and evaluate their ability to produce indole-3-acetic acid (IAA) and solubilize phosphate. Bacterial colony identification was performed using the streak plate method, IAA production was measured via spectrophotometry, and phosphate solubilization was assessed using the phosphate solubilization index (PSI). The results showed diverse bacterial isolates with distinct morphological, biochemical, and physiological characteristics. Several isolates exhibited strong IAA production, with B3 reaching the highest concentration (18.29 ppm) on the sixth day. Phosphate-solubilizing bacteria (PSB) were also identified, with B4 and S5 showing the highest PSI values of 6.06 and 5.3, respectively. These findings suggest that rhizosphere bacteria in the Sawah Tambak system have the potential to enhance rice growth by improving phosphorus availability and producing plant growth hormones, contributing to sustainable and environmentally friendly rice cultivation
Optimasi Durasi pada Sistem Irigasi Tetes dengan Sensor Kelembaban dan Suhu Tanah Menggunakan Logika Fuzzy Takagi-Sugeno Ardiansyah, Heri; Shodiq, Muhammad; Mahbubillah, M. Ainul; Agustina, Rohmatin
Techné : Jurnal Ilmiah Elektroteknika Vol. 24 No. 1 (2025)
Publisher : Fakultas Teknik Elektronika dan Komputer Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31358/techne.v24i1.562

Abstract

Aspek utama dari setiap budidaya tanaman adalah proses irigasi, yang harus dikelola dengan tepat. Salah satu metode penting yang banyak digunakan adalah irigasi tetes. Penelitian ini menggunakan metode logika fuzzy Takagi-Sugeno untuk mengoptimalkan durasi irigasi menggunakan sensor kelembaban tanah dan suhu pada sistem irigasi tetes. Data yang dihasilkan dari 10 set pengukuran dengan kelembaban tanah rata-rata 60,14%, suhu 32,84°C, dan durasi irigasi 321 detik. Metode logika fuzzy Takagi-Sugeno menganalisis data dan menghasilkan durasi irigasi yang optimal berdasarkan aturan fuzzy yang telah ditentukan. Hasil penelitian menunjukkan bahwa metode ini dapat menangani variabilitas kondisi lingkungan dengan menyesuaikan durasi irigasi sesuai dengan kebutuhan tanaman. Penerapan metode logika fuzzy Takagi-Sugeno pada sistem irigasi tetes otomatis ini telah meningkatkan efisiensi penggunaan air, mengurangi biaya operasional, dan mendukung keberlanjutan pertanian dalam inovasi pertanian modern. Namun, diperlukan penelitian lebih lanjut dengan data yang lebih luas dari berbagai kondisi lingkungan dan jenis tanaman untuk mendapatkan kesimpulan yang lebih akurat dan komprehensif.
In silico research of anti-CHIKF phytoconstituent-based from Physalis peruviana leaves via molecular docking and dynamics analyses Setiyowati, Putri Ayu Ika; Mahbubillah, M. Ainul; Aini, Nur Sofiatul; Rachmawati, Yuanita
Genbinesia Journal of Biology Vol. 3 No. 1 (2023): November 2023
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v3i1.62

Abstract

Chikungunya fever (CHIKF) is an infectious disease that has similar symptoms with dengue fever (DF). Several drugs have been offered to treat both dengue (DENV) and chikungunya virus (CHIKV). Investigating anti-CHIKF potential from nearby plants is one strategy to produce potential drug to reduce CHIKF in endemic countries. Physalis peruviana is one the promising object to be new anti-CHIKV drug candidate. This study aimed to analyze the anti-CHIKV agents from leaf parts of P. peruviana. Ligand and protein samples were collected from multiple sources. The phytoconstituents were evaluated their drug-likeness properties throughout SwissADME webservers. Selected ligands then docked via PyRX and measured the output by binding affinity. Visualization of the best outputs was carried out using BIOVIA Discovery Studio. CABS-flex was carried out to screen the RMSF of molecular dynamics activity of the best complex. The result showed that 1,2-benzenecarboxylic acid had the lowest binding affinity following suramin as control with -5.1 and -11.1 kcal/mol after targeting E2 domain protein of CHIKV. This led to the conclusion that 1,2-benzenecarboxylic acid could be forecast as predictive anti-CHIKF therapeutic candidate. Additional in vitro and in vivo studies are needed to validate this outcome.
Co-Authors Aini, Nur Sofiatul Aisyah Hadi Ramadani Aisyah, Neelam Ayuni, Rieke Dwi Dwi Zulianti Fika Nur Fitriana Putri Bukhori Heri Ardiansyah Khakim, Arsanul Muhammad Badrut Tamam Muhammad Badrut Tamam Muhammad Shodiq, Muhammad Nynda Ayu Nadira Savitri Redjeki, Endah Sri Rofiatun Solekha Rohmah, Laila Ainur Rohmatin Agustina Savitri, Nynda Ayu Nadira Setiyowati, Putri Ayu Ika Tamam, Muhammad Badrut Yuanita Rachmawati