Tita Widjayanti
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The Influence of Trap and Refugia Plants on Populations and Attacks of Leaf-Miner Flies Liriomyza sp. on Shallots Cultivation Setiawati, Lia; Mudjiono, Gatot; Widjayanti, Tita
Journal of Tropical Plant Protection Vol. 4 No. 1 (2023)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2023.004.1.1

Abstract

Leaf miners (Liriomyza sp.) are one of the pests affecting shallot plants. Attacks by Liriomyza sp. on shallot plants lead to tuber rot and leaves drying out, resembling burn damage. One control method is to utilize trap plants and refugia. Trap plants are specific species that attract pests, diverting them from the main crop. Refugia, on the other hand, are areas where pests can seek shelter, reducing their impact on the main crop. This study aimed to assess the impact of trap plants and refugia on the population and attacks of Liriomyza sp. in shallot crops. The research was conducted from June to September 2021 at the experimental field of the Faculty of Agriculture in Jatimulyo Village, Lowokwaru District, Malang City, East Java. Observation parameters included the population of Liriomyza sp., damage intensity, population of natural enemies, plant height, and number of leaves. The results indicated that different types of trap plants significantly influenced the population of Liriomyza sp. and damage intensity compared to the control treatment. However, different trap plant types did not significantly affect the population of natural enemies. The natural enemies identified included Vespidae, Coenosia sp., and Opius sp. Additionally, various trap plant types did not significantly impact plant height and the number of leaves.
The Effectiveness Test of Trichoderma harzianum, Mycorrhiza and Bacteria B8 on the Growth of Phytophthora nicotianae in Pineapple Plants (Ananas comosus L. Merr) at PT.Great Giant Pineapple Larasati, Candra Violita; Syamsulhadi, Mochammad; Widjayanti, Tita
Journal of Tropical Plant Protection Vol. 4 No. 2 (2023)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2023.004.2.5

Abstract

This study aims to determine the potential of biological agents to inhibit the pathogen P.  nicotianae that causes liver rot and the effect of biological agents on the growth of pineapple plants at PT. Great Giant Pineapple. The research was carried out at the Experimental Field and Research and Development Protection Laboratory of PT. Great Giant Pineapple, Terbanggi Besar, Central Lampung from September to November 2022. The research was carried out in several stages, namely the preparation stage and the implementation stage. The preparation stage includes land preparation, breeding and application of T. harzianum, preparation of liquid culture of T. harzianum and B8 bacteria, dipping crowns, and selection of crowns and tagging. While the implementation phase includes planting, application of T. harzianum, mycorrhiza, and B8 bacteria, observation of disease occurrence level, antagonist test, observation of new leaf growth, and plant observation. The data obtained were analyzed using the T-test, which compared the control plot with the treatment plot (T. harzianum, mycorrhiza, and B8 bacteria). The results showed that the application of the biological agent T. harzianum had a significant effect on suppressing the pathogen P. nicotianae that causes heart rot in pineapple plants in vitro. In addition, the application of T. harzianum, mycorrhiza, and B8 bacteria significantly affected the growth of the number of tillers, d-Leaf height, and d-Leaf index.
The Effectiveness of Three Strains of Beauveria bassiana (Balsamo) Vuillemin Isolate with Different Densities and Tobacco Leaf Extract on Plutella xylostella Linnaeus (Lepidoptera: Plutellidae) Hadi, Mochammad Syamsul; Siringoringo, Intan; Widjayanti, Tita; Setiawan, Yogo
Plantropica: Journal of Agricultural Science Vol. 8 No. 1 (2023)
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/10.21776/ub.jpt.2023.008.1.9

Abstract

This study aims to determine the pathogenicity and feeding inhibition of three B. bassiana isolate strains with different densities and concentrations of EDT (Tobacco Leaf Extract) in controlling P. xylostella. Research conducted in the Lab. Brenjonk Biological Agency and Lab. BPTPH Pasuruan from October to December 2022. This study used a Randomized Block Design (RAK) to examine the mortality and feeding inhibition of B. basiana and EDT strain isolates against P. xylostella. And probit analysis to find out the LC50 value and LT50 value. In this study, there were 17 treatments consisting of 15 larvae in each treatment and 4 repetitions were carried out. The results showed that the highest mortality percentage of P. xylostella was found in B. bassiana isolates of Bojonegoro and Jatisari strains at densities of B. bassiana 108 and 1010. The lowest LC50 value of B. bassiana isolates was the Bojonegoro strain and the EDT LC50 value was 43.79 ml/lt. Meanwhile, the lowest LT50 value was found in the EDT treatment at 28.40 hours. Based on the test results, it was also known that B. bassiana and EDT had potent feeding inhibition against P. xylostella. However, the use of EDT in controlling P. xylostella was less effective and efficient than the application of B. bassiana.
Exploration of Symbiont Bacteria Potentially Degrading Chlorpyrifos Insecticide in the Digestive Tract of Beet Armyworm (Spodoptera exigua) in Kediri Regency Widjayanti, Tita; Hadi, Mochammad Syamsul; Tantri, Zalfa Meiska
Plantropica: Journal of Agricultural Science Vol. 8 No. 2 (2023)
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpt.2023.008.2.10

Abstract

Spodoptera exigua Hubner (Lepidoptera: Noctuidae) is an important pest on shallot crops and cosmopolitan with a fast life cycle. In controlling S. exigua farmers use chemical insecticides such as chlorpyrifos. Intensive application of insecticides causes resistance of S. exigua. Resistance can occur due to several factors, one of which is biological factors. In biological factors, there is a symbiosis between pests and beneficial bacteria called symbiont bacteria. It is suspected that symbiont bacteria symbiotic with S. exigua can degrade chlorpyrifos. The research was conducted from December 2022 to May 2023. The research began with a site survey and sampling in three sub-districts in Kediri Regency, namely Plemahan District, Kepung District, and Badas District. Furthermore, samples of S. exigua were studied at the Pesticide Toxicology Laboratory, Brawijaya University. The research conducted includes (1) exploration, isolation, and purification of symbiont bacteria in the digestive tract of S. exigua, (2) bioassay test of symbiont bacteria that have the potential to degrade chlorpyrifos, and (3) characterization of bacteria that have the potential to degrade chlorpyrifos. The results of the exploration of symbiont bacteria in the digestive tract of S. exigua obtained as many as 45 isolates of bacteria capable of growing in NA + chlorpyrifos media. Furthermore, from bioassay testing, 14 isolates of bacteria that have the potential to degrade chlorpyrifos insects. The characterization results showed that bacterial isolates that have the potential to degrade chlorpyrifos insecticides come from the genera Pantoea, Erwinia, and Coryneform.