Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Universa Medicina
M. Aris Widodo
Bagian Farmakologi, Fakultas Kedokteran Universitas Brawijaya Malang
Agriculture, Biological Sciences & Forestry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
Decreased osteoblasts and increased osteoclasts in rats after coal dust exposure Akbar, Izaak Zoelkarnain; Kania, Nia; Setiawan, Bambang; Nurdiana, Nurdiana; Widodo, M. Aris
Universa Medicina Vol 30, No 2 (2011)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2011.v30.73-79

Abstract

Bone remodeling is a physiological process of cortical and trabecular bone reconstruction, with initial bone resorption, by osteoclasts and concurrent bone formation by osteoblasts. Oxidative stress due to coal dust exposure is not only found in the lungs, but also in the circulation or systemically. The aim of this study was to determine the effect of oxidative stress from coal dust exposure on the number of osteoblasts and osteoclasts in rats. In this experimental study, four groups were evaluated: control; coal dust exposure at 6.25 mg/m3 for 28 days; coal dust exposure at 12.5 mg/m3 for 28 days; coal dust exposure at 25 mg/m3 for 28 days (all exposures were given daily for one hour). Circulatory oxidative stress was measured by malondialdehyde level. Osteoblast and osteoclast numbers were counted by light microscopic examination of distal femoral cross-sections stained with hematoxylin eosin. This study showed that malondialdehyde levels were significantly increased in coal dust exposure groups, in comparison with the control group (p<0.05). There were also significantly decreased numbers of osteoblasts (p<0.05) and significantly increased numbers of osteoclasts (p<0.05) numbers in coal dust exposure groups, as compared with the control group. No correlations were found between malondialdehyde levels (oxidative stress) and respective numbers of osteoblasts and osteoclasts in all coal dust exposure groups (p>0.05). Coal dust exposure increased malondialdehyde level and osteoclast numbers, and decreased osteoblast numbers, but no correlation was found between oxidative stress (caused by coal dust exposure) and osteoblast and osteoclast numbers.
Ambon Banana-Tree Sap stimulated Endothelial Cell Migration in Human Umbilical Vein Endothelial Cells (HUVECs) Induced with Inflammatory Mediator IL-1 Wulan, Provisia Marthalita Yuning; Dorothy, Maria Juliana; Alinda, Resha; Sofiana, Kristianingrum Dian; Prihardina, Bunga; Permaasari, Nur; Widodo, M. Aris
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4517.627 KB)

Abstract

Ambon banana-tree (Musa paradisiaca var.sapientum) sap (ABT sap) contains active substances that can stimulate the wound healing process such as: flavonoid, saponin, ascorbic acid. Those substances are needed in an important phase of wound healing called angiogenesis through activation of Vascular Endothelial Growth Factor (VEGF) pathway that stimulates actin reorganization in endothelial cell migration and proliferation. The aim of this study is to evaluate the effectiveness of banana-tree sap in stimulating cells migration, VEGF and nitric oxide (NO) level in Human Umbilical Vein Endothelial Cells (HUVECs) culture that induced by pro-inflammatory cytokine IL-1 (Interleukin-1). The ABT sap was obtained by pressing a fresh Ambon banana stem, then was mixed with culture medium into 0.125%, 0.25% and 0.5% concentration. The ABT sap then was used for treating HUVECs cell that induced by IL-1 200 ng/mL for 48-hours. The result of this study shows that Ambon banana-tree sap increased HUVECs cell migration. However, after 48-hours treatment with ABT sap showed no difference in the VEGF and NO level in the culture media. These results suggested ABT sap able to increase migration to HUVECs that promising for wound healing agent. Therefore, the further research to elucidate the detailed mechanism and identify the active compound warrant to be done.
ENZIM LIPOPROTEIN LIPASE SUATU ALTERNATIF PEMERIKSAAN GANGGUAN METABOLISME LEMAK PADA PENDERITA DM TIPE 2 IN VITRO Handayani, Dian; Am, Aulani’; Soeadmadji, Djoko W.; Widodo, M. Aris
Jurnal Kedokteran Brawijaya Vol 19, No 2 (2003)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.256 KB) | DOI: 10.21776/ub.jkb.2003.019.02.3

Abstract

ABSTRACT The biggest population of Diabetes Mellitus is type 2 diabetes. Dyslipidemia is frequently found in type 2 diabetic patients, which is characterized by increased TG plasma levels and decreased HDL. Increased TG levels may be due to LPL activity disturbance, which then  lead to impaired lipoprotein metabolism, such as decreased hydrolyzing TGs in VLDL and Chylomicron. LPL is an enzyme which activities are influenced by several factors such as temperature, pH, incubation period, and substrate concentration. LPL attached to vascular endothelium, and can be removed from it by giving heparin intravenous. Heparin acts as lipotropic agent which promote plasma lipid transfer to lipid deposit by secreting lipolitic enzyme such as LPL. After giving information  about the objective of this study on the possible site effect of the blood obtaining technique, the patient was asked  to give her voluntary consent. Heparin IV 60 IU/Kg BB was given 15 minute before blood sample was drawn. Obtain from aid type 2 diabetes individual and aid from non diabetic control subject. To determine optimal LPL action pH variations (6, 6.4, 6.8 and 7), temperature  (30, 32, 35 and 37)oC,
Effect of Tetrodotoxin from Crude Puffer Fish (Tetraodon fluviatilis) Liver Extract on Intracellular Calcium Level and Apoptosis of HeLa Cell Culture Untario, Natanael; Dewi, Titik Cinthia; Widodo, M. Aris; Rahaju, Pudji
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.04

Abstract

Cervical cancer is the third most commonly diagnosed cancer and fourth leading cause of women death with 8% of total death caused by cancer in women in 2008. Tetrodotoxin (TTX) is a potent neurotoxin found in inner organs puffer fish, with the specific mechanism of sodium channel blocking, and widely used for research purposes. Previous reports claimed that TTX has the capability of inhibiting the metastatic process of cancer and apoptotic effect. Studies also show that apoptosis is a process involving the increase of intracellular calcium level, yet the connection between TTX and increase of intracellular calcium level, therefore triggering apoptosis, has not been established. This is an experimental study with post test only control group design, carried out by exposing HeLa cell culture to a crude liver extract of a puffer fish species, Tetraodon fluviatilis. Crude puffer fish liver extract is administered into HeLa cell culture well in different concentrations 10-4, 10-2, and 10-1. Intracellular calcium level and apoptosis were then measured after 18 hours of incubation. Measurements of intracellular calcium level were done by using CLSM with Fura-2AM staining, and apoptosis by using flowcytometry with Annexin V/PI.  The result shows that there is a significant difference between samples both in intracellular calcium (p < 0.05) and apoptosis (p < 0,05). Both intracellular calcium and apoptosis levels are proportional to liver fish extract concentration. Pearson’s correlation test shows correlation between treatment and intracellular calcium levels (p = 0.000), between treatment and apoptosis (p = 0.002), but not between intracellular calcium and apoptosis (p = 0.05). These results suggest that TTX induces an increase in intracellular calcium level and apoptosis, but calcium pathway is not the sole cause of the apoptosis. 
Perubahan Gambaran Histopatologi Paru pada Paparan Debu Batubara Memakai Alat Model 2010 Kania, Nia; Setiawan, Bambang; Nurdiana, Nurdiana; Widodo, M. Aris; Kusuma, HMS Chandra
Majalah Kedokteran Bandung Vol 43, No 3
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tambang batubara di permukaan tanah mendorong pembuatan alat paparan debu batubara skala laboratorium. Optimasi alat paparan debu batubara model 2010 dilakukan untuk menentukan lokasi akumulasi debu batubara serta perubahan histopatologi paru. Penelitian eksperimental dilakukan pada kontrol (K), paparan debu batubara 14 hari (BB1), dan debu batubara 28 hari (BB2). Karakterisasi debu batubara dilakukan dengan scanning electron microscope (SEM) dan X-ray fluorescence (XRF) di Laboratorium Fisika Universitas Negeri Malang. Pemeriksaan histopatologis paru dilakukan di Laboratorium Patologi Anatomi RSUD Ulin Banjarmasin. Penelitian dilakukan periode Agustus−November 2010. Scanning electron microscope paru menunjukkan diameter partikel <10 mm dan akumulasi partikel di alveolus. Parenkim paru berupa struktur alveolus tipis (K), penebalan alveolus dan edema epitel edematous (BB1), peningkatan edematous dan penyempitan rongga alveolus (BB2). Epitel bronkus dilapisi epitel silindris, epitel goblet, sel radang yang minimal (K), perpanjangan epitel silindris, hiperplasia epitel goblet dan mukus (BB1). Sel epitel menjadi menipis, lebih banyak mukus dan morfologi epitel menjadi tidak jelas (BB2). Epitel bronkoalveolus dilapisi epitel silindris, minimal sel goblet dan sel radang (K). Hiperplasia epitel goblet yang mendominasi disertai mukus (BB1). Epitel silindris dengan proliferasi sel goblet, mukus, taburan sel radang, dan fibrosis (BB2). Simpulan, alat paparan model 2010 memicu akumulasi debu batubara di alveolus serta perubahan histopatologi berupa inflamasi, hiperplasia sel goblet, dan fibrosis. [MKB. 2011;43(3):127–33]. Kata kunci: Alveolus, debu batubara, histopatologi, paruLung Histopatology Changed in Coal Dust Exposure with Model 2010EquipmentCoal mining on surface ground stimulate to create coal dust exposure equipment on laboratory scale. Optimation of model 2010 coal dust exposure equipment focus on coal dust accumulation and histopathologic of lung. This experimental study was done in control (K), coal dust exposure for 14 days (BB1), and coal dust exposure for 28 days (BB2). Coal dust characterization was done by scanning electron microscope (SEM) and X-ray fluorescence in Physics Laboratory Malang State of University. Histopathologic analysis was done in Pathologic Laboratory Ulin General Hospital. Research was done August−November 2010. Lung SEM showed particle diameter 10 mm and particle accumulated in alveolus. Lung parenchym showed thin alveolus structure (K), thickenning of alveolus and edematous epithelial (BB1), increased edematous and narrower alveolus space (BB2). Epithelial in bronchus layering by cylindrical and goblet epithelial, inflammation cell (K), elongation of cylindrical epithelial, hyperplasia goblet epithelial and mucous (BB1). Epithelial became thick, more mucous, and epithelial morphology became unclear (BB2). Bronchoalveolus epithelial layering by cylindrical epithelial, minimal goblet cell and inflammation cell (K). Goblet cell hyperplasia and mucous (BB1). Cylindrical epithelial with goblet cell proliferation, mucous inflammation cell, and fibrosis (BB2). In conclusions, coal dust exposure with model 2010 equipment trigger coal dust accumulation in avelous and histopathologic changes of inflamation, goblet cell hyperplasia, and fibrotic. [MKB. 2011;43(3):127–33].Key words: Alveolus, coal dust, histopathologic, lung DOI: http://dx.doi.org/10.15395/mkb.v43n3.58
Paparan Debu Batubara Subkronik pada Peroksidasi Lipid dan Kadar Gula Darah Tikus Diabetes Melitus Yuwono, Agus; Setiawan, Bambang; Kania, Nia; Widodo, M. Aris
Majalah Kedokteran Bandung Vol 43, No 4
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Di Kalimantan Selatan, prevalensi penderita diabetes melitus sebesar 11,1%. Kalimantan Selatan merupakan provinsi dengan tambang batubara tersebar di seluruh wilayah. Hal ini menjadikan penderita diabetes melitus terpapar debu batubara. Penelitian ini bertujuan untuk mengukur peroksidasi lipid akibat paparan debu batubara subkronik dan pengaruhnya pada kadar gula darah tikus diabetes melitus. Kelompok penelitian meliputi tikus Wistar diabetes melitus (P1), kelompok diabetes melitus + paparan debu batubara dosis 12,5 mg/m3 1 jam/hari selama 28 hari (P2), dan kelompok diabetes melitus + paparan debu batubara dosis 25 mg/m3 1 jam/hari selama 28 hari (P3), masing-masing 6 ekor. Penelitian dilakukan mulai Agustus–Oktober 2010. Uji analysis of variance (ANOVA) terhadap kadar malondialdehid (MDA) plasma tidak didapatkan peningkatan secara bermakna antara kelompok perlakuan (p>0,05). Penurunan kadar gula untuk P1, P2, dan P3 berturut-turut sebesar 23,6%, 16,9%, dan 9,3%. Analisis uji t tidak berpasangan terhadap kadar gula darah tidak didapatkan perbedaan bermakna sebelum dan setelah perlakuan pada semua kelompok (p>0,05). Tidak terdapat korelasi antara kadar MDA plasma dan kadar gula darah setelah perlakuan pada berbagai kelompok (p>0,05). Disimpulkan bahwa paparan debu batubara subkronik tidak meningkatkan peroksidasi lipid yang mempengaruhi kadar gula darah pada tikus diabetes melitus dan tidak ada korelasi antara kadar MDA dan glukosa darah. [MKB. 2011;43(4):189–92].Kata kunci: Debu batubara, diabetes melitus, hiperglikemia, peroksidasi lipidSubchronic Coal Dust Exposure on Lipid Peroxidation and Blood Glucose Level in Diabetes Mellitus RatIn South Kalimantan, prevalence of diabetes mellitus was 11.1%. South Kalimantan is a province with coal mine spread in all districts. This condition induce coal dust exposure on diabetes mellitus patients. Aim of this study was to measure lipid peroxidation by subchronic coal dust exposure and its effect on glucose level in Wistar rats model of diabetes mellitus. Group included diabetes mellitus Wistar rats (P1), diabetes mellitus + coal dust exposure at dose 12.5 mg/m3 1 hour/day for 28 days (P2) and diabetes mellitus + coal dust exposure at dose 25 mg/m3 1 hour/day for 28 days (P3) on 6 rats, respectively. This research was done from August–October 2010. Analysis of variance (ANOVA) test concluded no significant differently on increased plasma malondialdehyde (MDA) level between all groups (p>0.05). Percentage of blood glucose level decreased 23.6%, 16.9% and 9.3% for P1, P2, P3 group, respectively. Unpaired t test concluded that blood glucose level were not significant differently between pre and post treatment in all groups (p>0.05). There was no correlation between plasma MDA level and blood glucose level in all groups of exposure (p>0.05). In conclusions, that subchronic coal dust exposure doesn’t increase lipid peroxidation and no effect on blood glucose level in diabetes mellitus rats and no correlation between MDA dan blood glucose level. [MKB. 2011;43(4):189–92].Key words: Coal dust, diabetes mellitus, hyperglycemia, lipid peroxidation DOI: http://dx.doi.org/10.15395/mkb.v43n4.68
Co-Authors Agus Yuwono Alinda, Resha Aulani’ Am Bambang Setiawan Dewi, Titik Cinthia Dian Handayani Djoko W. Soeadmadji Dorothy, Maria Juliana HMS Chandra Kusuma Izaak Zoelkarnain Akbar, Izaak Zoelkarnain Nurdiana Nurdiana Permaasari, Nur Prihardina, Bunga Pudji Rahaju, Pudji Sofiana, Kristianingrum Dian Untario, Natanael Wulan, Provisia Marthalita Yuning Yundari, Yundari