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All Journal BERITA BIOLOGI Jurnal Kefarmasian Indonesia
Hendig Winarno
Laboratorium Bahan Kesehatan PATIR - BATAN
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Medicine & Pharmacology

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ANTIPROLIFERATIVE ACTIVITY OF OCTADECA-8,10,12-TRIYNOIC ACID AGAINST HUMAN CANCER CELL LINES [Antiproliferasi Asam Oktadeka-8,10,12-triunoat Terhadap Galur Sel Kanker Manusia] Winarno, Hendig
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (838.129 KB) | DOI: 10.14203/beritabiologi.v9i4.2003

Abstract

Antiproliferative activity test of octadeca-8,10,12-triynoic acid isolated from parasitic plant Scurrula airopurpurea (Bl.) Dans,against four kinds of human cancer cell lines, i.e: HeLa (human cervix epitheloid carcinoma),leukemia THP1,(human peripheral blood acute monocyte).carcinoma A549 (human lung carcinoma) and lymphoma HUT78 (human cutaneous T-cell lymphoma)was carried out.The results showed that octadeca-8.10,12-triynoic acid exhibits the antiproliterative activity against four kinds of human cancer cell lines with the 1C5I, value of 0.66. 0.86, 0.99 and 2.36 mg/nil for HeLa.leukemia THP1, lung carcinoma A549,and lymphoma HUT78,respectively, lower than 4 mg/ml. which is the antiproliferative activity threshold for pure isolate or compound.
Aktivitas Antiinflamasi Crude Extract Fukoidan dari Sargassum crassifolium pada Sel RAW 264.7 yang Diinduksi LPS Hikariastri, Pangartika; Winarno, Hendig; Kusmardi, Kusmardi; Laksmitawati, Dian Ratih; Abdillah, Syamsudin
Jurnal Kefarmasian Indonesia VOLUME 9, NOMOR 2, AGUSTUS 2019
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v9i2.1547

Abstract

Inflammation is known as the basic mechanism underlying various chronic diseases. Macrophage activation by inflammatory stimulus induces the release of inflammatory mediators, thus the mediators becoming a promising target of anti-inflammatory drug development. Previous studies indicated that fucoidan has anti-inflammatory activity by inhibiting the release of proinflammatory mediators. The aim of this study is determining anti-inflammatory activity of fucoidan crude extract form Sargassum crassifolium Garut waters by observing its effect on proinflammatory cytokines TNF-α, IL-1β, dan IL-6. Fucoidan is a polysaccharide substance which has various characteristics, depending on the source and the extraction method which is influencing its bioactivity. Sargassum crassifolium collected from Garut-West Java is extracted using diluted HCl 0,1 M and precipitated with ethanol to obtain fucoidan crude extract. The crude extract is tested on LPS-induced RAW 264.7 cells to evaluate its effect on TNF-α, IL-1β, and IL-6 level using ELISA method. The result showed that fucoidan crude extract decreased the level of TNF-α by the dose of 25 and 50 μg/ml, and decreased the level of IL-1β and IL-6 by the dose of 25 μg/ml. The dose of 50 μg/ml failed to inhibit IL-1β and IL-6 production. This study showed that fucoidan crude extract derived from S. crassifolium has anti-inflammatory activity to RAW 264.7 cells by the dose of 25 μg/ml.
Co-Authors . Syamsudin Dian Ratih Laksmitawati Ermin Katrin Hikariastri, Pangartika Kusmardi Kusmardi Rini Mardikawati