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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Journal of Indonesian Tourism and Development Studies Biotropika Journal of Fisheries & Marine
ekwan nofa wiratno
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Social Sciences

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Pengaruh Gula Reduksi dan Total Nitrogen Terhadap Densitas dan Viabilitas Sel Saccharomyces cerevisiae dalam Fermentasi Etanol dari Molase wiratno, ekwan nofa; Ardyati, Tri
Biotropika: Journal of Tropical Biology Vol 1, No 1 (2013)
Publisher : Biotropika: Journal of Tropical Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.307 KB)

Abstract

Fermentasi etanol dipengaruhi oleh beberapa faktor, salah satu diantaranya adalah densitas dan viabilitas sel khamir. Penelitian ini bertujuan mengetahui pengaruh variasi total nitrogen dan gula reduksi terhadap densitas dan viabilitas sel Saccharomyces cerevisiae (SAF Instan) selama proses fermentasi etanol dari molase. Tahapan penelitian ini terdiri dari pengukuran kadar gula reduksi, total nitrogen, konsentrasi kalsium, penentuan densitas dan viabilitas sel khamir serta analisis data. Fermentasi dilakukan selama 72 jam dengan 3 ulangan. Perlakuan konsentrasi gula reduksi (GR) dan total nitrogen (N) yaitu GR 100 N 0, GR 100 N 6, GR 100 N 10, GR 125 N 0, GR 125 N 6 dan GR 125 N 10 g/l. Penghitungan viabilitas sel dilakukan setiap 24 jam. Viabilitas tertinggi adalah GR 125 N 0 (95,76 %) dan GR 100 N 6 (95,96 %). Penurunan viabilitas sel disebabkan oleh rendahnya perkembangbiakan sel khamir (tidak terjadi peningkatan jumlah sel secara signifikan (p>0,05)). Hal ini terjadi karena tingginya konsentrasi kalsium (0,21 %).
Isolation, Identification and Ethanol Production of Indigenous Yeast of Toddy Palm (Borassus Flabellifer L.) Juice From Tuban, East Java, Indonesia Wiratno, Ekwan Nofa; Rupilu, Novi Soleman
Biotropika: Journal of Tropical Biology Vol 6, No 1 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.075 KB) | DOI: 10.21776/ub.biotropika.2018.006.01.2

Abstract

This study aimed to obtain and identify the indigenous yeasts isolated from Borassus flabellifer L. juice and its potency in ethanol production. Steps of this study included isolation, enumeration, characterization, identification and ethanol production and measurement of ethanol concentrations and medium acidity during fermentation. The yeast total isolated from Borassus flabellifer L. juice was 5.06 x 105 CFU/mL and the isolate U2 was phenotypically identified as Candida tropicalis (95.7% ID and T = 1). The pH level of medium decreased during the ethanol fermentation. Yeast isolate U2 was able to produce ethanol by 140.1 g/L using 50 g/L of glucose and it was not significantly different with ethanol yield fermented by Saccharomyces cerevisiae (148.6 g/L).
Genomic and Haplotype Comparison of Butanol Producing Bacteria Based on 16S rDNA Wiratno, Ekwan Nofa; Suharjono, Suharjono; Wardani, Agustin Krisna
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.01

Abstract

High butanol demand for transportation was support to butanol development. Exploration of butanol producing bacteria using genome comparison and biogeography give role to butanol industrialization. Objective of this research are butanol production, genome comparison and haplotype analysis of butanol producing bacteria from Ranu Pani Lake sediment using 16S rDNA sequences. Highest butanol concentrations were resulted by Paenibacillus polymyxaRP 2.2 isolate (10.34 ± 0.00 g.l-1) then Bacillusmethylotrophicus RP 3.2 and Bacillusmethylotrophicus RP 7.2 isolate (10.11 ± 0.01 g.l-1 and 9.63 ± 0.01 g.l-1). Number of bases (T, C, A, G) of group 1 are similar, but different with group 2. Least G+C content is Clostridium saccharobutylicum Ox29 (51.35%) and highest is Bacillus methylotrophicus RP 7.2. Conserve region (1044 bp) of 16S rDNA higher then variative region (367 bp). The number of 319 bp is PIS whereas single tone as much as 48 bp. There are 17 conserves sequences. All of butanol producing bacterial sequences was clustered to 8 haplotype. Based on source of bacteria, there are three group of haplotype. Group A was isolated from Asia, group B was isolated from America and group C was isolated from Europe.
Post-Covid-19 Pandemic Awareness on The Use of Micro- and Nano Plastic and Efforts into Their Degradation - A Mini Review Wiratno, Ekwan Nofa; Rozdhl, Amira Azawani Mohd; Hanafi, Nafizatun Eliana Ali; Redzuan, Rabiatul Alia; Huyop, Fahrul Zaman
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.12

Abstract

Micro- and nanoplastic pollution possess a global threat and cause a future problem and needs greater attention. Its pollution is also exacerbated recently by the use of abundance of plastic polymers in efforts to prevent and handle COVID-19 pandemic at the global scale. This review covered the major concerns about the characteristic, effect and bioremediation of micro- and nanoplastics of post COVID-19. Based on size, microplastic is described as debris particles smaller than 5 mm whereas, nanoplastic is referred to any particles smaller than 100 nm. Micro- and nanoplastic are easily ingested by many aquatic organisms at different trophic levels. This ingestion caused negative health impacts to all living organisms. Microplastic direct effect on living organism for example mechanical injury, false satiation, declined growth, promoted immune response, energy loss, disrupted enzyme activity and production, decreased fecundity, production of oxidative stress, and mortality. Nanoplastic could enter the circulatory system and caused negative effects on the cellular and molecular levels. Bioremediation of microplastic by magnoliophyta, bacteria, fungus and algae on several polymer forms was previously reported, however, not many on nanoplastic biodegradation. Therefore, current review will focus on the characteristics, effect and bioremediation effort of micro- and nanoplastic.
Abundance and Phylogenetic Analysis of High-Density Polyethylene (HDPE) Biodegrading Bacteria from Brantas River, Malang City Wiratno, Ekwan Nofa; Sandra Wardani, Erika; Agustin, Nurmalita
Biotropika: Journal of Tropical Biology Vol. 12 No. 2 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.02.06

Abstract

Microplastic pollution has become a global concern after the COVID-19 pandemic. This requires efforts to resolve the various adverse effects of microplastic pollution in the aquatic environment. This study aimed to isolate, enumerate, and determine the potency of microplastic biodegrading bacteria from the Brantas River, Malang City. This research includes environmental factor measures, bacterial isolation, enumeration, biodegradation would be produced (qualitative and quantitative), DNA isolation, PCR, sequencing, phylogenetic analysis, and data analysis. The environmental conditions of the sampling locations are generally homogeneous. The river temperature observed ranged from 24.1 to 27.2 °C. Research locations that have acidity ranging from 7.35 to 8.16. The salinity of the Brantas River ranges from 205-306 ppm or 0.0205-0.306%. The conductivity of the Brantas River ranges from 410-612 µS/cm. Isolation produced six different bacterial isolates. The amount of each isolate varies in each sample. A qualitative microplastic biodegradation test was indicated by the growth of colonies in the plastic area. This result shows that the bacterial isolate uses plastic as a carbon source. All isolates grow around plastic. Quantitative tests using HDPE sheets show that not all isolates can biodegrade microplastics. Bacterial isolates capable of biodegrading were isolates K2 (5.41%), K22 (5.16%), and K5 (6.69%). Based on phylogenetic analysis, it is known that isolate K2 has a similarity of 36.4% to ON845428.1 Klebsiella sp. strain 214 37 z 3, and ON845427.1 Klebsiella sp. strain 213 37 z 1. Isolate K2 has a distance score of 1.268 to MZ642649.1 Klebsiella variicola strain PDW768. Isolate K22 has a similarity of 36.8% with KT895299.1 Enterobacter cloacae strain Jilu WG154. The distance score of isolate K22 showed values of 1.179 and 1.156 against MH796357.1 Enterobacter sp. strain 3C, and OQ813771.1 Enterobacter cloacae strain TBMAX59. Similarity and distance score values consistently showed that isolates K2 and K22 were not identified at the genus level. These two isolates have the potential to be recognized as new species.
Oxygenase Enzyme Activity and Compound Profile in Hydrocarbon Bioremediation by Pseudomonas aeruginosa and Rhodococcus erythropolis Consortium Yanuar , Adi Tiya; Amin, Abd. Aziz; Salamah , Lutfi Ni'matus; Sujadi, Frentina Murty; Wiratno, Ekwan Nofa; Dewi, Citra Satrya Utama; Kurniawan, Andi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.45528

Abstract

Abstract Hydrocarbon contamination is one of the most significant environmental problems, including in the marine environment. Several methods, such as chemical and physical remediation, have been constructed to remediate pollutants but remain side effects and have a relatively high cost. Bioremediation is an alternative method for degrading contaminants due to its eco-friendliness and low cost. During bioremediation, the enzyme oxygenase is produced by bacteria to degrade pollutants. However, the oxygenation enzyme's activity and compound profile during this bioremediation process are still sparsely explored. This study aimed to analyze oxygenation enzyme activity and compound profiles during the hydrocarbon bioremediation process by bacterial consortia of Pseudomonas aeruginosa and Rhodococcus erythopolis. The materials of this research were the oxygenase enzyme activity, compound profile, and amount of BOD and COD during the bioremediation process. The results showed that the monooxygenase enzyme has activity between 0.257 and 3.859 U/mL. Meanwhile, the dioxygenase enzyme has activity between 0.579 and 5.402 U/mL. The final incubation compound profile found that Hexadecanoic acid methyl ester (C17H34O2) decreased by up to 47.66%. Moreover, BOD and COD reductions were 68.59% and 67.51%, respectively. This study concluded that oxygenation enzymes were produced to degrade pollutant compounds during the hydrocarbon bioremediation process. Further research is needed to improve the effectiveness of bioremediation by enhancing enzyme activity. Highlight Research The potential of hydrocarbon-degrading bacteria (Rhodococcus erythropolis and Pseudomonas aeruginosa) for pollutant degradation in the marine environment. Enzyme activity of hydrocarbon-degrading bacteria and degradation profile of pollutants. The dynamics of BOD and COD during the bioremediation process.
Spatial Diversity of Macrobenthic in Ngenep Spring Due to Anthropogenic Activities Wiratno, Ekwan Nofa; Nurdiansyah, Rizky; Basyaruddin, M.; Riyanto, Slamet; Rupilu, Novie S.; Retnaningdyah, Catur
Journal of Indonesian Tourism and Development Studies Vol. 4 No. 1 (2016)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitode.2016.004.01.04

Abstract

This aims of this study are to determine the macrobenthic community structure between the drainage near residence and spring; and to determine effect of local environment on macrobenthic community structure at the Ngenep village. Field study was conducted in 3 drainage, i.e. residential drainage, spring and rice field irrigation. Macrobenthic were sampled by the surber net and hand net in three stations in each drainages. Abiotic factors were assessed at the same stations. Macrobenthic community structure and diversity was determined using Important value index, Shannon-Wiener index and Bray Curtis index. Cluster of benthics and abiotic factors were analysis using Principal Component Analysis (PCA). Abiotic data showed no statistical significance in 3 drainage (P>0.05) but showed increasing trend from spring to field irrigation and the highest at residential drainage. The 17 taxa of macrobenthic were found with high important value index are Melanoides tuberculata, larva's of Ephemeroptera and Plecoptera. Highest Shannon-Wiener index observed at irrigation (2.781). The cluster analysis of abiotic factor showed that spring was in different cluster with residential and irrigation, meanwhile, PCA analysis of macrobenthic showed each place was different cluster. Conclusion of this study is anthropogenic activities alter the diversity and community structure of macrobenthic. Keywords: Anthropogenic Activity, Macrobenthic, Ngenep.
Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiae Wiratno, Ekwan Nofa; Ardyati, Tri; Wardani, Agustin Krisna
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1123.739 KB) | DOI: 10.21776/ub.jels.2014.004.02.05

Abstract

Indonesia's oil production has declined, while demand for derivative products is increasing. Objective of this research are to understand effect of reducing sugar and total nitrogen variation to ethanol production and fermentation efficiency, cell viability, acidity, temperature, dissolved oxygen with molasses by Saccharomyces cerevisiae (SAF Instant). Step of this research consist of determination of reducing sugar, ethanol fermentation, total nitrogen determination, ethanol determination and data analysis. Treatment of reducing sugar (GR) and total nitrogen (N) (g.L-1) that are GR 100 N 0, GR 100 N 6, GR 100 N 10, GR 125 N 0, GR 125 N 6 and GR 125 N 10. Fermentation was carried out for 72 hours with three replications. Observation parameters every 24 hours are ethanol and reducing sugar concentration, temperature, acidity and dissolved oxygen. Highest ethanol resulted from GR 125 N 6 (3.68 g.L-1) and GR 100 N 6 (3.53 g.L-1). Low reducing sugar consumption inhibited by by-product of yeast metabolism and molasses chemical compound, lead leaves high sugar concentration (> 80 g.L-1). GR 100 N 6 and GR 125 N 6 have highest fermentation efficiency (69 and 57 %). There was no increase in temperature and decrease in pH significantly (α>0.05). Dissolved oxygen decreased significantly (α>0.05) at the early of fermentation and decrease until the end of fermentation. Total nitrogen 6 g g.L-1 has the highest fermentation efficiency. Keywords: ethanol, molasses, reducing sugar, Saccharomyces cerevisiae, total nitrogen
Co-Authors AGUSTIN KRISNA WARDANI Agustin, Nurmalita Amin, Abd. Aziz Andi Kurniawan Basyaruddin, M. Catur Retnaningdyah Dewi, Citra Satrya Utama Hanafi, Nafizatun Eliana Ali Huyop, Fahrul Zaman Jamhari Jamhari Redzuan, Rabiatul Alia Rizky Nurdiansyah Rozdhl, Amira Azawani Mohd Rupilu, Novi Soleman Rupilu, Novie S. Salamah , Lutfi Ni'matus Sandra Wardani, Erika Slamet Riyanto Sujadi, Frentina Murty Tri Ardyati Yanuar , Adi Tiya