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All Journal Jurnal Kimia Riset Food Technology and Halal Science Journal
Laurent Octaviana
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Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

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Kajian Fitokimia dan Aktivitas Antioksidan Jamur Tiram Putih (Pleurotus ostreatus) Vritta Amroini Wahyudi; Laurent Octaviana; Sutrisno Sutrisno
Food Technology and Halal Science Journal Vol. 3 No. 1 (2020): Januari
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.309 KB) | DOI: 10.22219/fths.v3i1.13062

Abstract

Processed products derived from mushrooms have been found and enjoyed by the community. One of the edible mushrooms used as traditional medicinal ingredients andalso, functional food is oyster mushrooms. Although oyster mushrooms have often been studied even using sophisticated instruments, some chemical laboratories that do not yet have sophisticated instruments still need literature data in the process of separation until pure isolates are obtained. This research was conducted to be the solution. The study uses descriptive methods by collecting data from the results of extraction, characterization, identification, and testing of antioxidant activity. Characterization includes retention factor (Rf) from elution of thin-layer chromatography (TLC), determination of melting point (to determine the purity of isolates), and solubility test (to determine the nature of polarity). The identification of isolates was done by analyzing the results of Infrared and UV-Vis spectra. Phytochemical studies of white oyster mushrooms can be done based on secondary metabolite screening, separation by extraction, and fractionation using organic solvents. Based on phytochemical screening, white oyster mushrooms are known to positively contain flavonoids, alkaloids, and terpenoids. The crude extract had an absorbance of 0.669 at 274 nm, the chloroform fraction had an absorbance of 0.827 at 267 nm, and pure isolates had an absorbance of 0.628 at 282 nm. A spectrum with a maximum wavelength of> 210 nm indicates a conjugated system. The longer the conjugated system, the more inactive in the UV region of 200- 360 nm (UV) but more active in the 360-729 nm (Visible) region (Worsfold et al, 2019). UV-Vis spectrum of methanol extract, chloroform fraction, and pure isolate showed wave crests in the region> 210 nm so that all three were shown to have a conjugated system.
Identification of Species and Fatty Acid Composition of Microalgae from Raas Beach Sendang Biru Malang and Their Potential as Biodiesel Feedstock Eli Hendrik Sanjaya; Alifah Novitasari; Parlan Parlan; Laurent Octaviana; Dermawan Afandy; Sitoresmi Prabaningtyas
Jurnal Kimia Riset Vol. 2 No. 1 (2017): Juni
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.015 KB) | DOI: 10.20473/jkr.v2i1.4449

Abstract

Biodiesel is one of the best renewable energy made from triglycerides. Microalgae has a high lipid content include triglycerides, so it can be used as raw material for biodiesel production. Malang has many of beaches which are potent to become sources of microalgae, several beaches are surrounding Sendang Biru. The aims of this research were to identify the species of microalgae from Raas Beach Sendang Biru, determine their yield of lipid and fatty acid composition, and analyse their potential to became feedstock of biodiesel production. This research consists of five stages: identification of microalgae, cultivation, harvesting, determination of the yield of microalgae lipid by soxhlet extraction method, and analysis of the fatty acid composition of lipid by GC-MS. The results showed that the species of microalgae are dominated by Euglena sp and small amount of Gloeocapsa. The lipid yield of dried microalgae is 7.5% and the fatty acids composition are dodecanoic acid (1.6%), tetradecanoic acid (1.3%), hexadecanoic acid (16.6%), 9,12-octadecadienoic acid (25.2%), 9-octadecenoic acid (29.8%), and octadecanoic acid (6.7%). Based on this results, it can be concluded  that the microalgaes from Raas Beach Sendang Biru are potential to become biodiesel feedstock but it still need optimization.
Co-Authors Alifah Novitasari Dermawan Afandy Eli Hendrik Sanjaya Parlan . Sitoresmi Prabaningtyas Sutrisno Sutrisno Vritta Amroini Wahyudi