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All Journal International Journal of Advances in Applied Sciences Indonesian Journal of Geography VALENSI Jurnal Ilmu Lingkungan Jurnal Ilmu Lingkungan Jurnal Pendidikan Ekonomi (JUPE) The Journal of Pure and Applied Chemistry Research Jurnal Kimia Riset Indonesian Journal of Chemistry Jurnal Kartika Kimia Fullerene Journal of Chemistry Bantenese Jurnal Pengabdian Masyarakat Jurnal Pengendalian Pencemaran Lingkungan (JPPL) Jurnal Pengabdian Masyarakat Biologi dan Sains Jurnal MIPA dan Pembelajarannya Innovation for Community Service Journal Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) Frontier Advances in Applied Science and Engineering

Eli Hendrik Sanjaya

Universitas Negeri Malang

Author-ID : 3008158

Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Other

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POTENSI TRIMIRISTIN BIJI PALA FAKFAK (Myristica argantea warb) DAN EKSTRAKSI KULIT BUAH NAGA MERAH SEBAGAI BAHAN ADITIF SABUN DALAM PENERAPAN KIMIA HIJAU: REVIEW ANALISIS Isma Yanti Vitarisma; Atikah Atikah; Yudhi Utomo; Eli Hendrik Sanjaya
Jurnal Ilmu Lingkungan Vol 16, No 1 (2022): Jurnal Ilmu Lingkungan
Publisher : Program Pascasarjana Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jil.16.1.p.87-99

Fakfak nutmeg seeds contain 6% of essential oil and 11-34% of fat. Trimyristin contained in nutmeg seeds reaches 80%. The oil and fat content in the nutmeg seeds can be used as an ingredient for making soap, perfume and other industrial materials. Tools made of stainless steel can be used to distill the oil content in nutmeg seeds. Reflux system using ester which will then be purified using acetone and then tested again with gas chromatography to isolate and purify nutmeg essential oil. Extraction of anthocyanin pigments from red dragon fruit peel waste using maceration method using distilled water and 10% citric acid with a ratio of 1:6 which is a mixed solvent type. The test parameters used pH values and absorbance values were measured using spectrophotometry with the UV-Visible method to determine and analyze the brightness level of the anthocyanin red dye content. The results of the content of trimyristin which can be potential as a soap additive and can be tested for anti-bacterial and anti-fungal properties combined with the principle of green chemistry.

INSERSI GEN pncA KE DALAM PLASMID pGEM-T Eli Hendrik Sanjaya
Jurnal Kimia Riset Vol. 1 No. 2 (2016): Desember
Publisher : Universitas Airlangga

Abstract. Multidrug-resistant tuberculosis (MDR-TB) is among the most worrisome elements of the pandemic of antibiotic resistance. As the first line drug, pyrazinamide is often used to treat TB desease so there are many case of TB resistant to pyrazinamide. The previous research show that pncA gene of isolate L20 MDR-TB have mutated T539C. That mutation propose as the cause of resistance M. tuberculosis to pyrazimanide at the genetic level. For make sure the resistance mechanism, we have to get the pure PZAse and crystalization so the 3D structure can be determined by X-ray defraction. The first step to get the pure PZAse is cloning the pncA gene to the plasmid. The aim of this research is to know that is the pncA gene can be cloned to pGEM-T plasmid. The prosedure for cloning the pncA gene to the pGEM-T plasmid is amplification, followed by insert the pncA gene to the pGEM-T plasmid, and transformation by a selection of blue and white colony. The last step are isolation plasmid recombinant (pGEM-T-pncA) followed by electrophoresis. The result of the research showed that pncA gene from isolate L20 was successfully cloned to pGEM-T plasmid. That was showed on blue and white colony and the result of isolation and electrophoresis pGEM-T-pncA. The electrophoregram showed that the length of pGEM-T-pncA from white colony is different with pGEM-T standart abaut 0,7 kb. It is similar with the length of pncA gene (0,72 kb). Keywords: kloning, pGEM-T, pncA gene, pyrazinamide (PZA).

Identification of Species and Fatty Acid Composition of Microalgae from Raas Beach Sendang Biru Malang and Their Potential as Biodiesel Feedstock Eli Hendrik Sanjaya; Alifah Novitasari; Parlan Parlan; Laurent Octaviana; Dermawan Afandy; Sitoresmi Prabaningtyas
Jurnal Kimia Riset Vol. 2 No. 1 (2017): Juni
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Biodiesel is one of the best renewable energy made from triglycerides. Microalgae has a high lipid content include triglycerides, so it can be used as raw material for biodiesel production. Malang has many of beaches which are potent to become sources of microalgae, several beaches are surrounding Sendang Biru. The aims of this research were to identify the species of microalgae from Raas Beach Sendang Biru, determine their yield of lipid and fatty acid composition, and analyse their potential to became feedstock of biodiesel production. This research consists of five stages: identification of microalgae, cultivation, harvesting, determination of the yield of microalgae lipid by soxhlet extraction method, and analysis of the fatty acid composition of lipid by GC-MS. The results showed that the species of microalgae are dominated by Euglena sp and small amount of Gloeocapsa. The lipid yield of dried microalgae is 7.5% and the fatty acids composition are dodecanoic acid (1.6%), tetradecanoic acid (1.3%), hexadecanoic acid (16.6%), 9,12-octadecadienoic acid (25.2%), 9-octadecenoic acid (29.8%), and octadecanoic acid (6.7%). Based on this results, it can be concluded that the microalgaes from Raas Beach Sendang Biru are potential to become biodiesel feedstock but it still need optimization.

Penggunaan Karbon Aktif dari Biji Kelor dan Berbagai Biomassa Lainnya dalam Mengatasi Pencemaran Air : Analisis Review Jacky Anggara Nenohai; Zelen S. Minata; Burhanuddin Ronggopuro; Eli Hendrik Sanjaya; Yudhi Utomo
Jurnal Ilmu Lingkungan Vol 21, No 1 (2023): January 2023
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.21.1.29-35

Tujuan penelitian ini adalah untuk mengetahui (1) penggunaan karbon aktif dari berbagai bahan alami dalam penjernihan air dan (2) penggunaan biji kelor dalam penjernihan air, dan (3) cara menganalisis logam berat dari hasil penjernihan air. Metode yang digunakan dalam penelitian ini yaitu metode telaah pustaka yang sistematis, atau biasa disebut dengan Systematic Literature Review (SLR) yang dimana hasilnya menggunakan database artikel online yaitu Google Schoolar. Hasil telaah dari artikel yang berkaitan dengan topik diperoleh bahwa penggunaan karbon aktif atau arang yang terbuat dari bahan alami seperti kayu kesambi, ampas tebu, tongkol jagung, kulit buah durian dan eceng gondok sangat efektif dalam penyerapan zat terlarut air, baik organik maupun anorganik dalam menghasilkan air yang jernih. Selain itu, penggunaan biji kelor juga sangat efektif sebagai biokoagulan dan bioflokulan dalam menurunkan pH, kesadahan, kekeruhan dan total padatan tersuspensi, sehingga memungkinkan munculnya rekomendasi bahwa air hasil penjernihan dengan menggunakan karbon aktif dari berbagai bahan alami dan serbuk biji kelor dapat dijadikan air minum.

Pengaruh Sosialisasi Bahaya dan Cara Pengelolaan Limbah Batik Terhadap Tingkat Pemahaman Pembatik Desa Sumberejo Evi Susanti; Eli Hendrik Sanjaya; Retno Wulandari; Muh Ade Artasasta; Zarin Nafasari; Muhammad Reza Pahlevi; Sarif Hidayat; Shinta Yuliana
Jurnal Pengabdian Masyarakat Biologi dan Sains Vol 1, No 2 (2022): Jurnal Pengabdian Masyarakat Biologi dan Sains
Publisher : Universitas Indraprasta PGRI

Desa Sumberejo merupakan salah satu desa binaan Universitas Negeri Malang yang berada di Kecamatan Gedangan Kabupaten Malang. Desa ini terdiri dari enam dusun yakni Dusun Sumbersari, Mulyosari, Krajan, Dokosari, Tlekung dan Sumberwangi. Desa Sumberejo dikenal sebagai desa penghasil batik, dengan branding “Batik Sujo”. Produksi Batik Sujo melibatkan ibu-ibu rumah tangga yang berasal dari berbagai dusun di Desa Sumberejo. UMKM ini memproduksi beberapa lembar kain batik dalam sehari dan setiap produksi menghasilkan limbah, khususnya limbah cair yang dapat menimbulkan pencemaran pada lingkungan. Edukasi mengenai dampak dan pengelolaan limbah cair batik perlu diketahui oleh semua pembatik, sehingga perlu dilakukan sosialisasi mengenai topik tersebut. Tujuan kegiatan ini untuk mengetahui dampak sosialisasi terhadap tingkat pemahaman mengenai bahaya limbah batik dan cara pengelolaannya. Metode penelitian menggunakan Quasi Experimental dengan One Group Pretest Posttest Design, dengan pengambilan subjek menggunakan metode Quota Sampling. Hasil penelitian menunjukkan bahwa sosialisasi berpengaruh terhadap Pembatik Sujo, karena terjadi peningkatan pemahaman mengenai dampak dan pengelolaan limbah batik meningkat 15%.

Edukasi dan Pelatihan Penerapan Proses Pengolahan Batik Sujo “Zero-Waste” Melalui Pemanfaatan Mikoremediasi Evi Susanti; Muh Ade Artasasta; Sinta Yuliana; Retno Wulandari; Eli Hendrik Sanjaya; Muhammad Ali Murtadho
Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) 2023: Sinergi Perguruan Tinggi dan Masyarakat Untuk Mendukung Pencapaian Empat Pilar Pembangunan Men
Publisher : Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Desa Sumberejo merupakan salah satu desa binaan Universitas Negeri Malang yang berada di Kecamatan Gedangan Kabupaten Malang. Salah satu potensi Desa Sumberejo yang tengah dikembangkan adalah industri rumahan pembuatan kain batik, yang dikenal dengan Batik Sujo. UMKM Batik Sujo memproduksi 30 potong kain batik per bulan, dan setiap produksinya menghasilkan limbah, khususnya limbah cair. Limbah cair berpotensi tinggi mengandung senyawa organik dan logam berat, sehingga dapat menimbulkan pencemaran jika dibuang langsung ke lingkungan. Sosialisasi dan penerapan mengenai transfer teknologi bioremediasi limbah cair batik Sujo menggunakan teknik mikoremediasi perlu dilakukan. Tujuan kegiatan ini untuk mengedukasi dan memberikan pelatihan pengolahan batik sujo tanpa menghasilkan limbah cair (zero-waste) melalui pemanfaatan mikoremediasi kapang pelapuk kayu. Efisiensi bentuk pengolahan ini dapat menghasilkan produk bermanfaat seperti media tanaman hias. Metode pengabdian dilakukan melalui tiga tahap yaitu edukasi, pelatihan, evaluasi. Ketiga tahapan tersebut dihimpun melalui data primer dan sekunder, dengan subjek para Pengrajin Batik Sujo sebanyak 8 peserta. Hasil survei pengabdian menunjukkan para peserta pengrajin batik sujo desa Sumberejo dapat memahami pemanfaatan mikoremediasi untuk dapat diterapkan dalam mengolah limbah cair menjadi media tanaman hias. Selain itu, hasil survei pelatihan pemanfaatan kapang pelapuk kayu sebagai mikoremediasi limbah cair batik sujo menjadi media tanaman hias dinilai efisien dan efektif dalam menanggulangi limbah cair pembuatan batik sujo. Teknik mikoremediasi terbukti efektif dalam mengelola limbah cair batik sujo menjadi zero-waste. Namun, dibutuhkan pengembangan lebih lanjut agar teknik mikoremediasi dengan menggunakan kapang pelapuk kayu dapat diaplikasikan dengan baik di masyarakat. Kata kunci: Limbah cair; Batik Sujo; Mikoremediasi

Pengaruh Inhibitor Terhadap Aktivitas Enzim Febriana, Silvia; Anggraini, Dyas; Lakapu, Erlita; Hidayati, Elvi; Kerinandhila, Kerinandhila; Riska N.M., Nofita; Hendrik Sanjaya, Eli
Jurnal MIPA dan Pembelajarannya Vol. 3 No. 5 (2023): Mei
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um067v3i52023p223-228

Pada dasarnya semua enzim adalah protein sehingga adanya perubahan kondisi lingkungan dapat memengaruhi struktur tiga dimensinya. Aktivitas enzim dipengaruhi oleh beberapa hal antara lain pH, suhu, konsentrasi substrat, konsentrasi enzim, dan adanya inhibitor enzim. Inhibitor enzim adalah molekul yang dapat berinteraksi dengan enzim yang dapat mengurangi laju reaksi yang dikatalisis enzim atau mencegah enzim bekerja secara normal. Untuk membuktikan adanya pengaruh inhibitor kompetitif terhadap aktivitas enzim dan seberapa besarnya pengaruhnya maka perlu dilakukan penelitian ini. Tahapan yang dilakukan pada penelitian ini meliputi pangamatan pengaruh adanya malonat terhadap aktivitas enzim suksinat dehidrogenase. Hasil penelitian menunjukkan adanya malonat yang berperan sebagai inhibitor kompetitif dapat menghambat kerja enzim suksinat dehidrogenase dalam mengorversi suksinat menjadi fumarat. Besarnya pengaruh malonat ditentukan oleh konsentrasi malonat. Semakin banyak konsentrasi malonat maka semakin lama kerja enzim suksinat dehidrogenase dalam mengkatalisis suatu reaksi.

Synthesis and Characterization of Calcium Oxide Nanoparticles and Their Application in the Adsorption of Indigo Carmine Maharani, Brigitta Stacia; Khoerunnisa, Fitri; Anwar, Budiman; Hendrawan, Hendrawan; Hamidah, Ida; Nurhayati, Mita; Sanjaya, Eli Hendrik
Jurnal Kartika Kimia Vol 7 No 1 (2024): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v7i1.253

This research aims to determine the optimum conditions for the synthesis of CaO nanoparticles using the coprecipitation method, determine the characteristics of the CaO nanoparticles synthesized, and determine the effect of the performance of CaO nanoparticles as an adsorbent for indigo carmine dyes. CaO nanoparticles were successfully synthesized using the coprecipitation method at the optimum conditions of 1 M NaOH concentration and formation temperature of 400+200℃ with the highest % yield reaching 74.56%. The successful formation of CaO nanoparticles was proven by the appearance of a 2-theta diffraction peak of 23.12°; 39.2°; 57.9°; and 67.2° which is identical to the lattice structure of CaO with hkl indices (200), (311), (422) and (600) and a crystal size of up to 4.96 nm. SEM images support the formation of CaO nanoparticles with an average particle size of 98.1 nm and a varying size distribution. The IR spectrum of the formation of CaO nanoparticles with the appearance of Ca-O peaks at wavelengths of 3640, 1400, 860, and 791 cm-1. The adsorption capacity of indigo carmine on CaO nanoparticles was greatest at an adsorbent dose of 10 mg, adsorbate concentration of 100 ppm and a contact time of 50 minutes. The most appropriate adsorption isotherm model and adsorption kinetics model was the Koble-Corrigan model and the pseudo second order model, respectively.

Exploration The Candidates of Xenobiotic Degrading Indigenous Bacteria from Probolinggo City Landfill by Using Next Generation Sequencing (NGS) Qodriyah, Nur Romadhona Lailatul; Sanjaya, Eli Hendrik; Wahab, Roswanira Abdul; Susanti, Evi
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 2, November 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i2.34316

Soil bacteria from tropical environments play a significant role in resolving various environmental issues, including biodegradation. Exploratory research on biodiversity is crucial to develop and harness the potential of different types of soil bacteria that are highly abundant. The bacterial diversity in landfills is typically high due to the decomposition of organic and inorganic waste, creating a favorable medium for the growth and development of soil bacteria. This study aims to assess the candidates of xenobiotic degrading indigenous bacteria from the Probolinggo City landfill using Next Generation Sequencing (NGS) method. The research stages include: 1) sampling, 2) isolation of genomic DNA from samples using the ZymoBIOMICS DNA MiniPrep Kit from Zymo Research, 3) amplification of isolated DNA with primers 16S 27F – 1429R, 4) sequencing the results of DNA amplification with NGS, 5) downstream analysis of the results using software Pavian Krona Tools, and 6) narrative analysis review to identify the candidates of xenobiotic degrading indigenous bacteria. The results show that soil samples from the Probolinggo City landfill exhibited a high diversity of bacterial communities. Based on NGS analysis, 2400 bacterial species were identified, comprising 56 genera, 17 orders, 4 classes, and 4 phyla, with respective abundances of Proteobacteria (70%), Firmicutes (15%), Planctomycetes (2%), and Cyanobacteria (0,3%). Based on the narrative analysis review, several bacteria in the Probolinggo City landfill exhibited potential as: 1) polypropylene-degrading bacteria, including Bacillus cereus, B. licheniformis and B. thuringiensis. 2) styrofoam degrading bacteria, namely Bacillus amyloliquefaciens, Bacillus cereus, Bacillus firmus and Pseudomonas aeruginosa. 3) total ammonia nitrogen (TAN) reducing bacteria, including Bacillus megaterium. 4) pesticide degrading bacteria Profenofos and Chlorantraniliprole, including Bacillus stearothermophilus. and 5) tannic acid degrading bacteria, including Pantoea dispersa. These results indicate that the Probolinggo City landfill is a good habitat for various xenobiotic-degrading bacteria, then the isolation of specific bacteria can be designed using an appropriate selective medium.

The Effectiveness of Aerobic and Anaerobic Methods During Start-up in Biological Shrimp Pond Wastewater Treatment Sanjaya, Eli Hendrik; Amara, Aulia; Hengky Pamungkas, Yahya; Ricky Wijaya, Anugrah; Santoso, Aman; Yulistyorini, Anie; Li, Yu-You; Chen, Hong; Fadhil Md Din, Mohd
The Journal of Pure and Applied Chemistry Research Vol. 12 No. 3 (2023): September-December 2023
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2023.012.03.3309

One of the aquaculture wastes is shrimp pond wastewater (SPW). Generally, SPW comes from the rest of shrimp feed, shrimp dung, and died shrimp during the cultivation. As SPW is organic waste, biological wastewater treatment is the best choice. Biological method has low operating costs and environmentally friendly. There are two kind of biological methods, namely aerobic and anaerobic. Both methods have advantages and disadvantages. In this research, the performance of aerobic and anaerobic methods in treating SPW were investigated using batch experiment for more than 200 days. The results showed that the performance of the aerobic system in the initial phase was better than that of the anaerobic system, especially, in phase one which is the adaptation phase. In the first phase, COD, carbohydrate and protein removal in the aerobic system were 25.93%, 75.2% and 88.59%, while in the anaerobic system were 12.82%, 36.03% and 51.01%. In phases 5 to 10, both systems have similar performance with the optimum performance in phase 9 with 6 times substrate dilution. In this optimum phase, the COD removal values are 90% and 85% for aerobic and anaerobic systems, respectively. Meanwhile, the removal of carbohydrates and protein from both systems is about 95%.

Co-Authors Abrilia, Putri Riza Ahmad Munjin Nasih Alifah Novitasari Alvionita, Mieke Aman Santoso Amara, Aulia Anggraini, Dyas Anie Yulistyorini Annifah, Riri Uswatun Anugrah Ricky Wijaya Ardyansyah, Ananta Armaini, Armaini Asep Kadarohman Ashfiya, Putri Sayyida Aziz, Hafiz Aji Billah, Egi Nursari Budiman Anwar Burhanuddin Ronggopuro Deni Ainur Rokhim Dermawan Afandy Dewi, Ratna Kumala Edy, Duwi Leksono Evi Susanti Evy Damayanthi Fadhil Md Din, Mohd Fadila Abna Swari Febriana, Silvia Fitri Khoerunnisa Hakiki, A.Riyan Rahman Hayuni Retno Widarti Hendrawan Hendrawan Hengky Pamungkas, Yahya Hidayati, Elvi Hidiyah, Tabita May Hong Chen Iasya, Yurin Karunia Apsha Albaina Ida Hamidah Iklima, Ilma Kanzu Imam Alfianto Isma Yanti Vitarisma Jacky Anggara Nenohai Kerinandhila, Kerinandhila Lakapu, Erlita Laurent Octaviana Li, Yu-You Lilik Eka Radiati Listyo Yudha Irawan Maharani, Brigitta Stacia Muh Ade Artasasta Muhammad Ali Murtadho Muhammad Reza Pahlevi Muhammad Roy Asrori Mujahidah, Amiroh Nabilah Muntholib Munzil Nurhayati, Mita Parlan . Prastiwi, Mellinia Regina Putri, Nanda Regita Qodriyah, Nur Romadhona Lailatul Retno Wulandari Riska N.M., Nofita RR. Ella Evrita Hestiandari Sarif Hidayat Shinta Yuliana Shrestha, Rajendra Prasad Sinta Yuliana Siti Mutmainah Siti Sendari Sitoresmi Prabaningtyas Sofinila, Mida Fitri Agasty Suharti Suharti Sumari Sumari Syamsul Bachri Taupik Hidayat, Taupik Wahab, Roswanira Abdul Wijayanti, Chandra Yahmin Yahmin Yaya Sonjaya Yudhi Utomo YULIANA, SHINTA Zarin Nafasari Zelen S. Minata

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