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The Effect of Red Fruit Oil (Pandanus conoideus) to the Histophysiology of Rat (Rattus norvegicus) Liver Exposed to Cigarette Smoke Kio, Albertina Dhiu; Saraswati, Tyas Rini; Yuniwarti, Enny Yusuf Wachidah
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12822

Free radicals in cigarette smoke may interfere the balanced of body molecules and cause the damage of hepatic cells. This issues may be prevented by using red fruit oil (Pandanus conoideus L) due to its antioxidant content. This study aimed to evaluate the structure and physiological conditions of liver when the red fruit oil given to the rat that exposed by cigarette smoke. This study carried out for 35 days with female white rats as the sample. The samples were 20 female white rats divided into four groups: P0 (negative control), P1 (positive control), P2 (exposed by cigarette smoke with administration of red fruit oil 0,1 ml/BB), P3 (exposed by cigarette smoke with administration of red fruit oil 0,2 ml/weight). The data of ALT, AST, cell diameter, and hepatic weight were analyzed by using analysis of variants (ANOVA). The results showed that administration of red fruit oil containing antioxidants can reduce levels of ALT, AST, hepatocyte diameter, and increase liver weight, although the difference is not significant compared to the control group and does not affect the eating and drinking behavior so as not to affect the bodys metabolism. The conclusion of red fruit oil can reduce ALT, AST, hepatocyte diameter and increase hepatic weight due to exposure to cigarette smoke, although the difference is not significant to the control group, and does not affect the eating and drinking behavior, so good for consumption because it gives a beneficial effect on health liver organ.

Sex Determination in Male and Female Melopsittacus undulates using a Morphometric Method Baehaqi, Ibnu; Saraswati, Tyas Rini; Yuniwarti, Enny Yusuf Wachidah
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Parakeets (Melopsittacus undulates) are classified parrot order Psittaciformes. Parakeets are monomorphic birds whose sex is difficult to be distinguished. Sex identification is very important for breeding efforts in order to increase the parakeet population. External morphology was determined to identify the sex of the birds. This research was conducted to determine the sex of parakeets (male and female). This study used five male and five female parakeets aged 4 months as the study objects. This study used the quantitative and qualitative method. Results of this study was verified by performing surgery to determine the sex of parakeet based on their reproductive organs. The t-test results of morphometric characteristics showed no significant different in the length of body, upper bill, lower bill, wing, tail, femur, tibial-tarsus, tarsometatarsus and digits as well as the body weight between male and female parakeets. The color of the cere was useful to accurately determine sex in parakeets (blue in males and white in females). This research provided information to the public about the differences between male and female parakeets for the selection of good broodstock in order to increase their population in captivity.

Antihyperglycemic Activity of Aqueous Extract of Insulin Leaves (Tithonia diversifolia) on Hyperglycemic Rats (Rattus norvegicus) Sari, Anisa Rachma; Saraswati, Tyas Rini; Yuniwarti, Enny Yusuf Wachidah
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Insulin leave (Tithonia diversifolia (Hemsl.) A. Gray) empirically used by people as antihyperglycemic drugs. The study was aimed to evaluate antihyperglycemic activity as well as to determine the most optimum dose of T. diversifolia to reduce blood glucose levels. Hyperglycemic condition was induced to male Rattus norvegicus rats by intraperitoneal injection of alloxan at a dose of 150 mg/kg bw (body weight). In this study, completely randomized design was performed with three treatment groups and five times repetition. Group P1 received glibenclamide 10 mg/kg bw, P2 received aqueous extract of T. diversifolia at a dose of 150 mg/kg bw, P3 received aqueous extract of T. diversifolia at a dose of 300 mg/kg bw. Administration of both glibenclamide and aqueous extract of T. diversifolia was conducted orally for 28 days. Data were analyzed using Anova and Duncan’s test with 95% confidence level (α = 0.05). The results showed that the mean percentage of decrease in blood glucose levels, drink intake and body weight of all treatment groups were not significantly different (p>0.05). Data of feed intake showed that P1 was significantly different from P2 (p<0.05), but P2 was not significantly different from P3 (p>0.05). Based on the result of this research, it was found that the low doses of aqueous extract of T. diversifolia has the same ability to decrease blood glucose level compared to glibenclamide. Futhermore, this study provide some information that can be used as preclinical analysis to determine effective doses of aqueous extract of T. diversifolia to decrease blood glucose levels.

Profile of SGPT and SGOT on Male Rats (Rattus norvegicus) Hyperglycemic After Giving Insulin Leaf Extract (Tithonia diversifolia) Yuneldi, Rizki Fitrawan; Saraswati, Tyas Rini; Yuniwarti, Enny Yusuf Wachidah
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

The leaves of insulin (Tithonia diversifolia (Hemsl.) A. Gray) are native bush plants from Central America. This plant in Indonesia is often called paitan or kembang bulan. The aim of this research was to know the profile of SGPT and SGOT of male white rat (Rattus norvegicus L.) hyperglycemic after giving insulin leaf extract (Tithonia diversifolia (Hemsl.) A. Gray). This study used 20 male Wistar rats which were divided randomly into 5 groups of treatment. Those were P0 as a group of rats which was not given alloxan and insulin leaf extract, P1 as a group of rats which was given alloxan without insulin leaf extract, P2 as a group of rats which was given alloxan and glibenclamide 2 mg/Body Weight (BW)/day, P3 as a group of rats which was given alloxan and insulin leaf extract 30 mg/BW/day, P4 as a group of rats which was given alloxan and insulin leaf extract 60 mg/BW/day. Each treatment was repeated as many as 4 replications. The results of this study indicated that there was no significant differences in all variables, namely levels of SGPT, SGOT, liver weight, body weight and Hepatosomatic index (HSI), so it can be concluded that giving insulin leaf extract (Tithonia diversifolia (Hemsl.) A. Gray) of 30 mg/BW/day and 60 mg/BW/day could improve liver function of hyperglycemic male white rats (R. norvegicus L.).

Effects of Kersen Juice and Lakum Leaf Extract on Lipid Profile of White Rats With Hyperlipidemia Sopandi, Didin; Saraswati, Tyas Rini; Yuniwarti, Enny Yusuf Wachidah
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20813

Kersen fruit and Lakum leaf contain substances that are potential as an antihyperlipidemic. The aim of this research was to analyze the effects of Kersen juice (Mutingia calabura L.) with dosage 0.2 ml/200 g BW and lakum leaf extract (Cayratia trifolia L.) with dosage of 40 mg/200 g BW on lipid profile of white rats (Rattus norvegicus L.) with hyperlipidemia. This study used a Complete Randomized Design (CRD). This study used twenty adult male Wistar rats which were divided into five groups : P0 as a control group, P1 as a High Feed Diet (HFD) control group, P2 as a HFD group treated with Kersen juice with dosage of 0.2 ml/200 g BW, P3 as a HFD group treated with Lakum leaf extract with dosage of 40 mg/200 g BW and P4 as a HFD group treated with simvastatin. The treatments were given orally for 28 days. The parameters of this study were the level of cholesterol, HDL, LDL and triglyceride, as well as body weight, and food consumption. One-way ANOVA test was performed to determine the significant differences p<0.05 between groups followed by Duncan test with a 95% significant level. The results showed that compared to Kersen juice with dosage of 0.2 ml/200 g BW, administration of Lakum leaf extract with dosage of 40 mg/200 g BW was more effective in lowering cholesterol level, triglyceride and increasing HDL level, however, they were not effective in lowering LDL in rats with hyperlipidemia. This result shows that kersen and lakum leaf could be an alternative to prevent and avoid further effects of high cholesterol in people with hyperlipidemia.

Rasio Otot-Tulang Ekstremitas Posterior Ayam Pedaging Setelah Pemberian Teh Kombucha Dalam Air Minum Kumala, Amelia Setya Nur; Djaelani, Muhammad Anwar; Yuniwarti, Enny Yusuf Wachidah
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Kombucha tea beverage obtained by fermenting sweetened green tea for 12 days with Acetobacter xylinum and Saccharomyces which produce various kinds of organic acids, vitamins, and acts as a probiotic. Kombucha tea role as growth promoter is to enhance the metabolic processes in the digestive system of broiler, so that the nutrients can be absorbed and fulfilled properly for optimal growth and development. The research was conducted from September to October 2014 in the Laboratory of Biological Structure and Function, Diponegoro University. Posterior limb (femur-tibiotarsus) meat-bone ratio analysis was used to investigate the effects of kombucha tea on the productivity of broiler chicken. A total of 20 broiler chickens were randomly divided into 4 treatment concentrations ( 0%, 10%, 20%, and 40% of kombucha tea in drinking water) for 32 days. Statistical analysis using ANOVA at 0,05 significance level and Duncanâ€™s Multiple Range Test showed significantly different results on body weight and water intake, while feed intake, meat weight, bone weight and meat-bone ratio showed no significant results. It can be concluded from this study that the decrease of broiler chicken body weight due to consumption of 40% kombucha tea concentration in drinking water, do not cause a decrease in posterior limb meat and bone weight so that the posterior limb meat-bone ratio were not significantly different.Â Key words: Kombucha tea, broiler chicken, posterior limb, meat-bone ratio.

Efek Pemberian Virgin Coconut Oil Dan Olive Oil Dalam Pakan Terhadap Pertumbuhan Tulang Femur Tikus Putih Jantan (Rattus norvegicus) Suhartatik, S; Yuniwarti, Enny Yusuf Wachidah; Djaelani, Muhammad Anwar
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

This research aimed to annalyse the effects of virgin coconut oil and olive oil on the length, diameter and weight of the femur bone in white male rat (Rattus norvegicus). This research used 25 white male rats and consists of 5 treatments with 5 replications. This research used the treatment P0: as a control, P1: feed with an addition of 5 ml virgin coconut oil / kg feed, P2: feed with an addition of 10 ml virgin coconut oil / kg of feed, P3: feed with an addition of 5 ml olive oil / kg feed and P4: feed withÂ an addition of 10 ml olive oil / kg feed. This research used a method called Completely Randomized Design (CRD). The variables measured in this research are the weight, length and diameter of white male rats. The data was analyzed using Analysis of Variance (ANOVA) at 95% level. The results showed that the growth of femur bone in male rat does not have different response after giving virgin coconut oil and olive oil.Â Â

Perbandingan Kualitas Daging Itik Magelang, Itik Pengging Dan Itik Tegal Hidayati, Nuke Nur; Yuniwarti, Enny Yusuf Wachidah; Isdadiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Local ducks in Indonesia there was several kinds, for example Tegal ducks, Magelang ducks and Pengging duck each having morphology different of duck. Research on the quality meat of ducks that includes ash content, water content, carbohydrate content, fat content, protein content, and muscle cell diameter of femoral ducks important to know quality meat of each duck. This study aimed to analyzed the differences types of ducks on the chemical content of meat and femoral muscle histology of diameter cell at Tegal ducks, Magelang ducks and Pengging ducks. Ducks were used in this study maintained in the Central Breeding and Raising Ducks Work Unit Non Ruminant Livestock Banyubiru, Ambarawa. The duck six month old was analyzed chemical content of meat and measuring diameter of femoral muscle cells. Duck samples taken from three different types of ducks that Tegal duck,Pengging duck and Magelang duck, of each type of sample taken many ducks as 6 tails. Data were analyzed using analysis of variance (ANOVA). If the data were analyzed contained a real difference, continued by the Least Significant Differences Test (LSDT) at 95% confidence level. The results showed that no significant difference in all variables, from the result has been concluded that differences type of ducks not affect the chemical contents of duck meat and diameter of femoral muscle cells.Â Keywords: Local Ducks in Central Java, Quality of Meat, Muscle Cell Diameter Femoral Ducks.

Mikroanatomi Hepar Tikus Putih Setelah Pemberian Berbagai Kadar Vco dan Olive Oil Yuniwarti, Enny Yusuf Wachidah; Djaelani, Muhammad Anwar
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 24, No 1 (2016): Volume 24, nomor 1, tahun 2016
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

This research aimed to determine differences in Â hepatocyte cells after feeding saturated fatty acid and unsaturated fatty acid. Elevated level of fatty acid may contribute to damage of celluar hepatocytes. This research used 25 white rat. The method applied was Completely Randomized Design in which the factor was five levels of VCO and Olive Oil namely P1, P2, P3, P4 and P5. P1was the control group, P2 was a group with 10 mL Olive Oil /kg feed. P3 was a group with 5 mL Olive Oil /kg feed. P4 was a group with 10 mL VCO /kg feed. P5 was a group with 5 mL VCO /kg feed.Â Each group repeated five times. Feed and water were given ad libitum for four weeks. The result showed that there was no significant between treatments. Â Keywords : white rat,hepar microanatomy, olive oil, vco

KADAR TRIGLISERID TIKUS PUTIH SETELAH PEMBERIAN BERBAGAI KADAR VCO DAN OLIVE OIL Yuniwarti, Enny Yusuf Wachidah
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

This research aimed to determine differences in Â triglyceride levels after feeding saturated fatty acid and unsaturated fatty acid. Elevated level of triglycerides can lead to cardiovascular disease.This research used 25 white rat. The method applied was Completely Randomized Design in which the factor was five levels of VCO and Olive Oil namely P1, P2, P3, P4 and P5. P1was the control group, P2 was a group with 5 mL VCO/kg feed. P3 was a group with 10 mL VCO/kg feed. P4 was a group with 5 mL Olive Oil/kg feed. P5 was a group with 10 mL Olive Oil/kg feed.Â Each group repeated five times. Feed and water were given ad libitum for four weeks. The result showed that there was no significant between treatments. Â Keywords : white rat, triglyceride level, olive oil, VCO

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