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L.M. Yusiati
Faculty of Animal Science, Gadjah Mada University, Jl. Fauna No.3, Bulaksumur, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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COMPARATIVE STUDY ON ANGIOTENSIN CONVERTING ENZYME INHIBITORY ACTIVITY OF HYDROLYSATE OF MEAT PROTEIN OF INDONESIAN LOCAL LIVESTOCKS Jamhari, J.; Yusiati, L.M.; Suryanto, E.; Cahyanto, M.N.; Erwanto, Y.; Muguruma, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 1 (2013): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.1.27-33

Abstract

The experiment was conducted to investigate the angiotensin converting enzyme (ACE) inhibitoryactivity of hydrolysate in meat protein of Bali cattle, Kacang goat, native chicken, and local duck. Themeats of Bali cattle, Kacang goat, native chicken, and local duck were used in this study. The meatswere ground using food processor added with aquadest to obtain meat extract. The meat extracts werethen hydrolyzed using protease enzymes to obtain hydrolysate of meat protein. Protein concentration ofmeat extract and hydrolysate of meat protein were determined, and were confirmed by sodium dodecylsulfate - poly acrylamide gel electrophoresis (SDS-PAGE). ACE inhibitory activity of hydrolysate ofmeat protein derived from Bali cattle, Kacang goat, native chicken, and local duck was also determined.The results showed that protein concentration of hydrolysate of meat protein of Bali cattle, Kacang goat,native chicken, and local duck meat was significantly higher than their meat extracts. SDS-PAGEanalysis indicated that hydrolysate of meat protein of Bali cattle, Kacang goat, native chicken, and localduck had more peptides with lower molecular weight, compared to their meat extracts. Hydrolysate ofmeat protein of Bali cattle, Kacang goat, native chicken, and local duck had potencies in inhibiting ACEactivity, so it will potentially reduce blood pressure.
PURIFICATION OF ANGIOTENSIN CONVERTING ENZYME INHIBITORY PEPTIDE DERIVED FROM KACANG GOAT MEAT PROTEIN HYDROLYSATE Jamhari, J.; Yusiati, L.M.; Suryanto, E.; Cahyanto, M.N.; Erwanto, Y.; Muguruma, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 4 (2013): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.4.239-246

Abstract

The objective of this study was to identify the Angiotensin Converting Enzyme (ACE) inhibitorypeptide derived from Kacang goat meat protein hydrolysate. Kacang goat meat loin section washydrolyzed with pepsin, trypsin and chymotrypsin. Protein hydrolysate of Kacang goat meat was thentested the protein concentration and ACE inhibitory activity. ACE inhibitory peptide of the proteinhydrolysate was purified through several steps of purification by column SEP-PAK Plus C18 Cartridgeand RP-HPLC using a Cosmosil column 5PE-SM, 4.6 x 250　mm. The sequence of amino acid of ACEinhibitory peptide was identified by amino acid sequencer. The results showed that amino acidssequence of ACE inhibitory peptide derived from protein hydrolysate of Kacang goat meat was leu-thrglu-ala-pro-leu-asn-pro-lys-ala-arg- asn-glu-lys. It had a molecular weight (MW) of 1581 and occurredat the position of 20th to 33rd residues of b-actin of goat meat protein (Capra hircus). The ACE inhibitoryactivity (IC50) of the peptide was 190 mg/mL or 120 mM.
ISOLATION AND LIGNOCELLULOLYTIC ACTIVITIES OF FIBER-DIGESTING BACTERIA FROM DIGESTIVE TRACT OF TERMITE (Cryptothermes sp.) Tampoebolon, B.I.M.; Bachruddin, Z.; Yusiati, L.M.; Margino, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.224-234

Abstract

The objectives of this study were to obtain the fiber-digesting bacteria isolates from termitedigestive tract and to determine the optimum conditions of growth and production of cellulase, xylanaseand ligninase enzyme of isolate. The first study was conducted to isolate and select the fiber-digestingbacteria from the digestive tract of termites based on the highest activity of cellulolytic (S), xylanolytic(X) and lignolytic (L). The second study was optimation of the growth conditions of bacteria and theenzyme production due to effect of rice straw substrate and nitrogen. The material used were dry woodtermites, rice straw, and culture medium. The design used was a completely randomized factorial design,in which the first factor was rice straw substrate (1, 2, and 3% W/V), while the second factor wasnitrogen (0.1, 0.2 and 0.3% W/V). Variables measured were cellulase, xylanase and ligninase activities.Results of the first sudy showed that the isolates obtained consisted of 3 types, those were cellulolyticbacteria (S1, S2, and S3), 3 types of bacteria xylanolytic (X1, X2, and X3) and 3 types of bacteria lignolytic(L1, L2, and L3). Meanwhile, results of the second study showed that isolates of S2, X3, and L1 had thehighest activity, those were 1.894 U/mL, 1.722 U/mL and 0.314 U/mL, respectively. In conclusion, the addition of 1% level of rice straw substrate and 0.3% of nitrogen showed the highest enzyme activity oncellulase, xylanase and ligninase.
MICROBIOLOGICAL CHARACTERISTIC AND FERMENTABILITY OF KING GRASS (Pennisetum hybrid) SILAGE TREATED BY LACTIC ACID BACTERIAYEAST INOCULANTS CONSORTIUM COMBINED WITH RICE BRAN ADDITION Sofyan, A.; Yusiati, L.M.; Widyastuti, Y.; Utomo, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 4 (2011): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.4.265-272

Abstract

This research was conducted to evaluate the effect of inoculants consortium consisted ofLactobacillus plantarum (Lp) and Saccharomyces cerevisiae (Sc) and combined with rice bran additionon microbiological characteristic and fermentability of king grass (Pennisetum hybrid) silage. Effectivityof treatments was assessed by addition of inoculants (control, Lp, Lp+Sc) and level of rice bran (0, 5 and10%) which were arranged on Completely Randomized Design with Teatments Factors (3x3). Thevariables measured were total colonies of microbes (lactic acid bacteria, yeast and clostridia), total gasproduction, volatile fatty acids (VFA) and ammonia (NH3). Results showed that clostridial coloniescounted on silage treated by Lp and Lp+Sc around 37.5% and 68.7% in which lower than control.Addition of inoculants and rice bran increased fermentability of silage significantly (P<0.05), howevertreatment had no affected (P>0.05) on production of VFA and NH3. Gas production from silagefermentable fraction with inoculation of Lp (49.5 ml) and Lp+Sc (47.9 ml) higher than untreated silage(46.6 ml). It was concluded that the use of inoculants consortium consisted of L. plantarum and S.cerevisiae with rice bran addition improved fermentability and reduced clostridial colony in king grasssilage.
EFFECT OF SAPONIN AS DEFAUNATING AGENT ON IN VITRO RUMINAL FERMENTATION OF FORAGE AND CONCENTRATE Hanim, C.; Yusiati, L.M.; Alim, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 34, No 4 (2009): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.34.4.231-235

Abstract

nin level in fermentation medium (0, 0.1, 0.2, or 0.3 mg/ml), and many kinds of feed (king grass,rice bran, and king grass:rice bran, 60:40 w/w) on protozoa numbers, ammonia concentration, microbialprotein, pH and cellullase activity. Each treatment was consisted of three replicates. Fermentation wasdone in syringe and used in vitro gas production medium. The data obtained were analyzed by varianceanalysis using factorial design (4x3). The differences between mean values were analyzed by Duncan’snew multiple range test (DMRT). The result showed that protozoa numbers decreased 17.22, 42.73 and49.57% (P<0.01) for 0.1, 0.2, and 0.3 mg/ml saponin, respectively from 8.19×103/ml in the control. Theaddition of 0.1 mg/l saponin increased ammonia concentration from 33.04 mg/100 ml (without saponin)to 37.12 mg/100 ml (P<0,01), whereas the addition of 0.2 and 0.3 mg/ml saponin decreased ammoniaconcentrations by 1.69 and 16.50% (P<0.01) compared to the control. Microbial protein, cellullaseactivity and pH were not affected neither by saponin nor kind of feed. Protozoal numbers and ammoniaconcentration in the rumen were lower (P<0.01) with king grass as substrat than that with rice bran, orking grass: rice bran. In general, no interactions between saponin and kind of feed were observed, exceptfor ammonia concentration. It can be concluded that level of 0.2 mg/ml saponin have antimicrobialproperties, particularly in suppressing protozoa, which may prove beneficial to ruminal fermentation andmay lead to lower ruminal ammonia concentration, but it did not have negative effect on pH, microbialprotein and cellullase activity. King grass as a substrate decreases protozoa numbers and ammoniaconcentration.
Co-Authors A. Sofyan B.I.M. Tampoebolon C. Hanim E. Suryanto J. Jamhari M. Muguruma M.N. Cahyanto R. Utomo S. Alim S. Margino, S. Y. Erwanto Y. Widyastuti Z. Bachruddin