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All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal
Z. Bachruddin
Faculty of Animal Science, Gadjah Mada University, Jl. Fauna 3 Kampus UGM Bulaksumur, Yogyakarta
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy

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PRODUCTION AND CHARACTERIZATION OF CRUDE INTRACELLULER PHYTASE FROM RECOMBINANT BACTERIA pEAS1AMP Nuhriawangsa, A.M.P.; Bachruddin, Z.; Sajidan, S.; Wibowo, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 34, No 4 (2009): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.34.4.265-271

Abstract

This research was aimed at producing a crude intracellular phytase characterized from recombinantbacteria. The recombinant bacteria (pEAS1AMP) was produced by way of transforming pET-22b(+)+pEAS1 into competent E. coli BL21 and E. coli BL21(DE3) cells. Crude intracellular phytaseproduction was induced using 1,5 mM Isopropyl-β-D-thiogalactopyranosid (IPTG). Recombinantbacteria product and enzyme activity test followed the Sajidan method. E. coli BL21(+)pEAS1 and E.coli BL21 (DE3)(+)pEAS1 recombinant bacteria showed growth after 20 hours and 10 hours oftransformation. Phytase activity of E. coli BL21 (DE3)(+)+pEAS1 showed higher than those of E. coliBL21(+)+pEAS1. Crude intracellular phytase of pEAS1AMP recombinant bacteria has an optimumactivity at pH 5, 40oC, incubation period of 60 minutes, substrate concentration of 2%, molecular weight(MW) of 47.3 kDa, Km = 15.91 υM and Vm = 2.41 υM/second. Mg2+ acts as a cofactor but Fe3+ (10-4M) acts as an inhibitor.
CORN SUBSTITUTION USING FERMENTED SOLID CASSAVA-WASTE ON BROILER CHICKEN Ali-Mursyid, W.M.; Bachruddin, Z.; Zuprizal, Z.; Nur-Cahyanto, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 1 (2010): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.1.9-15

Abstract

The objective of the research was to examine unfermented solid cassava-waste (USCW) andfermented solid cassava-waste (FSCW) as the substitution of corn in broiler ration. Two hundred and tenbirds of one-week-male broiler chicken were divided into seven kinds of one-way treatment ofcompletely randomized design. Five replications were used for each treatment; there were six chickensin each replication. The substitution levels of corn in broiler ration by USCW and FSCW were 0%(control), 10, 20, and 30%. The result showed that corn substitution up to 20% using USCW and FSCWdid not affect the digestibility of feed (DF), digestibility of soluble protein (DSP) and crude protein(DCP), and digestibility of starch (DS). Substitution up to 30% using FSCW did not affect the DSP andDCP. However, substitution of USCW at the same level decreased DSP, DCP, DF, and DS. Cornsubstitution up to 20% using USCW and FSCW did not affect the feed intake (FI), body weight gain(BWG) and feed conversion ratio (FCR). Substitution up to 30% using FSCW did not affect the BWGand FCR. However, substitution of USCW at the same level decreased BWG and increased FCR. FSCWcan be used as a substitute for the corn up to 30% without affecting the performance and the values ofthe nutrient digestibility, meanwhile USCW is only able to substitute corn up to 20%.
THE INFLUENCE OF KAPOK (Ceiba pentandra) SEED OIL SUPPLEMENTATION ON CELLULOLYTIC ENZYME AND RUMEN MICROBIAL FERMENTATION ACTIVITY OF LOCAL SHEEP Widiyanto, W.; Soejono, M.; Bachruddin, Z.; Hartadi, H.; Surahmanto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 2 (2010): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.2.129-133

Abstract

This research was conducted to study the influence of kapok seed oil (KSO) supplementation oncellulolytic enzyme and microbial fermentation activity. Sheep rumen fluid was used as enzyme sourceand inoculant, whereas carboxymethylcellulose (CMC) was used as the substrate. There were 4 levels ofKSO supplementation as treatment, i.e. : 0% (T0), 5% (T1), 10% (T2), and 15% (T3). Two measuredvariables were reduced sugar production rate and gas fermentation production. The data were analyzedby analysis of variance in completely randomized design. The result showed that reduced sugarproduction rate in T0, T1, T2 and T3 treatment groups were 2.58; 2.93; 2.08 and 1.58 mg/gCMC/minute, respectively, whereas gas production were : 15.97; 13.26; 10.54 and 7.57 mg/g CMC,respectively. Kapok seed oil supplementation up to 5% DM of cellulose substrate (CMC) did notinfluence the ruminal cellulolytic enzyme activity. The KSO supplementation level 10% - 15%decreased the ruminal cellulolytic enzyme activity.
ISOLATION AND LIGNOCELLULOLYTIC ACTIVITIES OF FIBER-DIGESTING BACTERIA FROM DIGESTIVE TRACT OF TERMITE (Cryptothermes sp.) Tampoebolon, B.I.M.; Bachruddin, Z.; Yusiati, L.M.; Margino, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.224-234

Abstract

The objectives of this study were to obtain the fiber-digesting bacteria isolates from termitedigestive tract and to determine the optimum conditions of growth and production of cellulase, xylanaseand ligninase enzyme of isolate. The first study was conducted to isolate and select the fiber-digestingbacteria from the digestive tract of termites based on the highest activity of cellulolytic (S), xylanolytic(X) and lignolytic (L). The second study was optimation of the growth conditions of bacteria and theenzyme production due to effect of rice straw substrate and nitrogen. The material used were dry woodtermites, rice straw, and culture medium. The design used was a completely randomized factorial design,in which the first factor was rice straw substrate (1, 2, and 3% W/V), while the second factor wasnitrogen (0.1, 0.2 and 0.3% W/V). Variables measured were cellulase, xylanase and ligninase activities.Results of the first sudy showed that the isolates obtained consisted of 3 types, those were cellulolyticbacteria (S1, S2, and S3), 3 types of bacteria xylanolytic (X1, X2, and X3) and 3 types of bacteria lignolytic(L1, L2, and L3). Meanwhile, results of the second study showed that isolates of S2, X3, and L1 had thehighest activity, those were 1.894 U/mL, 1.722 U/mL and 0.314 U/mL, respectively. In conclusion, the addition of 1% level of rice straw substrate and 0.3% of nitrogen showed the highest enzyme activity oncellulase, xylanase and ligninase.
POTENCY OF LIGNOCELLULOSE DEGRADING BACTERIA ISOLATED FROM BUFFALO AND HORSE GASTROINTESTINAL TRACT AND ELEPHANT DUNG FOR FEED FIBER DEGRADATION Wahyudi, A.; Cahyanto, M.N.; Soejono, M.; Bachruddin, Z.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 1 (2010): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.1.34-41

Abstract

Lignin is limiting factor for cellulose and hemicellulose degradation in rumen. Isolation andselection bacteria from buffalo and horse gastrointestinal tract and elephant dung could be foundbacteria that have superiority to degrade lignin, xylan, and cellulose. Those animals were chosenbecause they were herbivores that consume low quality crude fiber as their main energy sources.Lignocellulose degrading bacteria were isolated by Hungate selective media, by using lignin (tannicacid), xylan, and cellulose as selective substrates. The morphological identification used an enrichmentmedia by measuring color, colony size, diffusion zone, clear zone, and biochemical identification usingproduction of ligninase, xylanase, and cellulase enzymes. The best lignocellulose degrading bacteriathen was determined by the morphological and biochemical character. This study showed thatlignocellulose degrading bacteria could be found in gastrointestinal tract of buffalo and horse, andelephant dung. Highest number colony was found in samples from buffalo's colon (376), followed byhorse's cecum (203), elephant’s dung (46), buffalo’s cecum (23), buffalo's rumen (9) and horse’s colon(7). The highest isolates activity of lignolytic, xylanolytic, and cellulolytic were reached by buffalo’scecum (7.64), horse's cecum (6.27), and buffalo’s colon (2.48). Meanwhile the highest enzymesproductivities were: buffalo’s cecum (0.0400 µmol), horse’s cecum (1.3912 µmol) and buffalo’s colon(0.1971 µmol). Based on morphologycal character and biochemical test, it could be concluded thatlignolytic from buffalo’s cecum, xylanolytic from horse’s cecum, and cellulolytic from buffalo’s colonwere the superior isolates and they were 99% analyzed as Enterococcus casseliflavus/gallinarumspecies.
Chemical and Physical Quality, Fermentation Characteristics, Aerobic Stability, and Ruminal Degradability of Sorghum Silage Inoculated with Lactiplantibacillus plantarum and Limosilactobacillus fermentum Fitriani, D.; Ardiansyah, M.; Kurniawati, A.; Bachruddin, Z.; Paradhipta, D. H. V.
Tropical Animal Science Journal Vol. 47 No. 4 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.4.483

Abstract

This study was carried out to determine the effect of homo (Lactiplantibacillus plantarum FNCC 0020) and hetero (Limosilactobacillus fermentum BN21) fermentative lactic acid bacteria on chemical compositions, fermentation characteristics, aerobic stability, and ruminal digestibility of sorghum (Sorghum bicolor L. Moench) silage. The sorghum forage was harvested at the milk ripening phase with a dry matter content of 25.6% and fermented for 100 days with different inoculants: treatments without inoculant (CON), L. plantarum (LP), L. fermentum (LF) as well as a mixture of LP and LF at a ratio of 1:1 (MIX). The experiment was conducted using a completely randomized design with 5 replications per treatment, and all inoculants were applied at 105 cfu/g of fresh forage. The results showed that LF silage caused a 66.3% reduction in cyanide acid content, the lowest mold count, and longer aerobic stability compared to LP and CON. The lowest pH (p<0.05) and highest organic matter digestibility (p<0.05) were obtained on LP silage, while the CON silage showed no significant difference. The LP and LF silage showed the highest total volatile fatty acid (p<0.05), while there was no significant between CON and others. The LF silage had the highest acetate and the lowest propionate (p<0.05). These results showed that L. fermentum was more effective in decreasing cyanide acid content and increasing the aerobic stability of sorghum silage, while L. plantarum was able to lower pH and reduce ammonia concentration.
Co-Authors A. Kurniawati A. Wahyudi A. Wibowo A.M.P. Nuhriawangsa B.I.M. Tampoebolon Fitriani, D. H. Hartadi L.M. Yusiati M. Ardiansyah M. Nur-Cahyanto M. Soejono M.N. Cahyanto Paradhipta, D. H. V. S. Margino, S. S. Surahmanto Sajidan Sajidan W.M. Ali-Mursyid Widiyanto Widiyanto Zuprizal (Zuprizal)