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All Journal Indonesian Journal of Forestry Research Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Indonesian Journal of Forestry Research
VIVI YUSKIANTI
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan. Jl. Palagan Tentara Pelajar Km 15 Purwobinangun, Pakem, Sleman, DI Yogyakarta 55582, Indonesia Telp: 0274-895954/0858-6869-2221, Fax: 0274-896080
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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DETECTION OF POLLEN FLOW IN THE SEEDLING SEED ORCHARD OF Acacia mangium USING DNA MARKER Yuskianti, Vivi; Isoda, Keiya
Indonesian Journal of Forestry Research Vol 10, No 1 (2013): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

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Abstract

Pollen pattern dispersal in seedling seed orchard (SSO) is an essential part of a tree-improvement program. Two SSOs of Acacia mangium in South Kalimantan and South Sumatra that represent similar resources in different environments were used in this study.  Genotypes of all trees and seeds from a subset of 10 mother trees in each orchard were determined for 12 microsatellite loci, and parentage analysis was carried out. The results shows that the pollen dispersal pattern in both SSOs decrease with distance from mother tree. Patterns of pollen dispersal, dispersal distance and cumulative frequency of pollen dispersal distance were similar in both SSOs. Random pollen dispersal were found in both SSOs. About 80% of all crosses were found within a 40-m distance range with the most frequent pollination distance between mother tree and male male parents was 0-10 m. No self-pollinated seed was detected. Application of all these aspects found in this study such as random pollen dispersal and the effective pollen dispersal distance can be useful for establishing seedling seed orchard, clonal seed orchard and in other tree improvement activities of A. mangium.
DETECTION OF POLLEN FLOW IN THE SEEDLING SEED ORCHARD OF Acacia mangium USING DNA MARKER Yuskianti, Vivi; Isoda, Keiya
Indonesian Journal of Forestry Research Vol 10, No 1 (2013): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2013.10.1.31-41

Abstract

Pollen pattern dispersal in seedling seed orchard (SSO) is an essential part of a tree-improvement program. Two SSOs of Acacia mangium in South Kalimantan and South Sumatra that represent similar resources in different environments were used in this study.  Genotypes of all trees and seeds from a subset of 10 mother trees in each orchard were determined for 12 microsatellite loci, and parentage analysis was carried out. The results shows that the pollen dispersal pattern in both SSOs decrease with distance from mother tree. Patterns of pollen dispersal, dispersal distance and cumulative frequency of pollen dispersal distance were similar in both SSOs. Random pollen dispersal were found in both SSOs. About 80% of all crosses were found within a 40-m distance range with the most frequent pollination distance between mother tree and male male parents was 0-10 m. No self-pollinated seed was detected. Application of all these aspects found in this study such as random pollen dispersal and the effective pollen dispersal distance can be useful for establishing seedling seed orchard, clonal seed orchard and in other tree improvement activities of A. mangium.
Eksplorasi Materi Genetik Kayu Merah (Pterocarpus indicus Willd) di Pulau Sumbawa, Nusa Tenggara Barat. Yuskianti, Vivi; Ismail, Burhan; Yuliah, Y
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2017: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Informasi habitat dan materi genetik yang dikoleksi dari suatu sebaran alami merupakan informasi penting untukmembantu upaya penyelamatan/konservasi suatu species. Kayu merah (Pterocarpus indicusWilld) dikenal sebagaijenis yang bernilai ekonomi tinggi telah dikategorikan oleh The IUCN Red List of Threatened Species sebagai jenisyang kondisinya mulai rawan (Vulnerable (VU A1d)). Upaya penyelamatan dapat dilakukan salah satunya denganpembangunan plot konservasi eks situ yang berisi koleksi materi genetik dari berbagai sebaran alami di Indonesia.Salah satu wilayah yang menjadi sebaran alami kayu merah adalah Pulau Sumbawa propinsi Nusa Tenggara Barat.Penelitian ini oleh karena itu bertujuan untuk mendapatkan informasi habitat dan materi genetik hasil eksplorasikayu merah dari sebaran alaminya di Pulau Sumbawa. Hasil penelitian menunjukkan bahwa kayu merah atau lebihdikenal oleh masyarakat lokal sebagai kayu nara dapat ditemukan di dua kabupaten di Pulau Sumbawa yaituKabupaten Bima dan Kabupaten Dompu. Sebaran kayu merah di Kabupaten Bima berada pada ketinggian tempatyang lebih tinggi (99-113 m dpl) dibandingkan dengan Kabupaten Dompu, sekitar pantai sampai dengan areaperbukitan (6,1-91 m dpl). Lokasi ditemukannya kayu merah juga umumnya berada pada kemiringan lahan di atas50o, yang cukup menyulitkan saat eksplorasi di lapangan. Dimensi pohon pohon induk yang dikoleksi materigenetiknya juga bervariasi dengan diameter terbesar sebanyak 95 cm dan tinggi bebas cabang antara 2-12 m. Hasileksplorasi di lapangan juga menunjukkan kondisi yang memprihatinkan karena keberadaan pohon kayu merahyang mulai terbatas akibat maraknya penebangan liar (illegal logging).
GENETIC DIVERSITY AND GENETIC RELATIONSHIP OF SENGON (Falcataria moluccana) REVEALED USING SINGLE NUCLEOTIDE POLYMORPHISM (SNP) MARKERS Yuskianti, Vivi; Shiraishi, Susumu
Indonesian Journal of Forestry Research Vol. 4 No. 2 (2017): Indonesian Journal of Forestry Research
Publisher : Association of Indonesian Forestry and Environment Researchers and Technicians

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59465/ijfr.2017.4.2.85-94

Abstract

Producing seeds in seed orchards that retain a broad genetic diversity is important for the continued development of a species. The aim of this study was to find out the genetic diversity of, and genetic relationships between several populations of sengon (Falcataria moluccana) in the Candiroto seed orchard in Indonesia. Analysis using twelve single nucleotide polymorphism (SNP) markers showed that the total population had a high level of genetic diversity (He = 0.359±0.128); the Wamena population in Papua had the highest level. The close genetic relationship between this and the population from East Java suggested that the latter may have been introduced from Wamena. There was also a close genetic relationship between the populations from Central and West Java and that from Mindanao Island in The Phillipines. The high genetic diversity of sengon in the Candiroto seed orchard provides a good basis for selection and breeding.
Co-Authors Burhan Ismail, Burhan KEIYA ISODA SUSUMU SHIRAISHI Yuliah, Y