Adi Winarto
Bagian Anatomi, Histologi dan Embriologi Fakultas Kedokteran Hewan Institut Pertanian Bogor

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MORFOLOGI KELENJAR AKSESORIS KELAMIN BIAWAK AIR (Varanus salvator bivittatus) JANTAN Mahfud .; Adi Winarto; Chairun Nisa
JURNAL KAJIAN VETERINER Vol 3 No 2 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i2.1046

Abstract

The study was aimed to describe the macromorphological and micromorphological aspect of the accessories gland of Male Varanus salvator bivittatus. Two adult male lizards with 45.60 cm SVL were used in this experiment. The animals were sacrificed by exsanguination under deep anesthetized and fixed in 4 % paraformaldehyde through perfusion and then its visceral site was observed. Histomorphological evaluation was obtained by paraffin preparation with section thickness of 3-4 μm then stained in Hematoxylin-Eosin (HE), Alcian Blue (AB) pH 2.5 and Periodic Acid Schiff (PAS). The result showed that accessories gland which was found at the dorsal of cloaca, specifically at the caudal end of deferent duct that was a hemipenile bulge. Microscopically, the accessories gland was identified as tubular mucous type gland which was resemble to bulbourethral gland in mammals. The cytoplasm of the secretory cells showed weak reaction, while strong reaction was observed in the luminal mucus which was stained by AB staining. In contrast, luminal mucus showed a weak reaction to PAS staining. In conclusion, V. salvator bivittatus has only one accessory gland and its microanatomical structure resembled the bulbourethral glands.