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All Journal HAYATI Journal of Biosciences Acta Biochimica Indonesiana Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Journal of Microbial Systematics and Biotechnology 3BIO: Journal of Biological Science, Technology and Management
Azzania Fibriani
Department of Biology, School of Life Sciences and Technology, Bandung Institute of Technology, Bandung 40132, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience

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Journal : HAYATI Journal of Biosciences

 1 
Implementation of Dimer-based Screening System in Escherichia coli BL21(DE3) for Selection of Actinomycetes Compounds as Anti-HIV Candidate Kenia Permata Sukma; Putri Cahya Destiani; Azzania Fibriani
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.192-203

Abstract

Actinomycetes are reported to have inhibitory activity against several types of Human Immunodeficiency Virus proteases, enzyme with major role in the process of maturation of the virus thus it can infect new cells. Therefore, exploration of Indonesia’s actinomycetes species is expected to be a breakthrough for HIV treatment. In this study, selection of anti-HIV candidate compounds was conducted using a dimer-based screening system on recombinant Escherichia coli BL21(DE3). The construct includes the fusion of the AraC DNA binding domain + HIV-1 protease as the regulator and the green fluorescence protein as the reporter. Confirmation of the plasmid construct was carried out by PCR which showed size of ~1,076 bp. Sequencing analysis proved 100% similarity and identity between construct used in this study and one previously designed. SDS-PAGE showed the presence of band in the size of ~24 kDa equal to the size of the fusion protein. Compounds BLH 1-12 (2) EA, MAE 1-13 EA, BLH 1-1 EA, BLH 7-5 MetA, LC 98 (1) EA, exhibited consistent and significant protease-HIV inhibitory activity at certain concentrations. Thus, in this study, dimer-based screening system is considered to be able to detect actinomycetes as a new anti-HIV candidate for the protease inhibitor group.
Successful Primer Picking and Pooling for the Design of Multiplex PCR Primers Specific to Pork, Beef, Chicken, and Rat DNA Kusumawaty, Diah; Faridah, Nurul; Fibriani, Azzania; Priyandoko, Didik; Dzikrina, Hanina; Puspitasari, Diah; Tallei, Trina Ekawati; Aryani, Any
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.678-686

Abstract

DNA markers and Multiplex-PCR have emerged as methods for species detection in processed meat products. The primary objective of this study is to design multiplex primer sequences for pork, rat, beef, and chicken, generating distinguishable amplicons through agarose gel electrophoresis for halal detection in processed meat products. Primer design involved utilizing mitochondrial genomic data and the NCBI-Primer BLAST site to obtain specific pork and beef primer sequences. In silico simulations, including single and multiplex-PCR, were conducted using Primer Pooler. In vitro validation encompassed Single-PCR and Multiplex-PCR annealing temperature optimization, using samples of chicken, beef, pork, and rat as well as processed meat products like meatballs, sausages, and nuggets. In vitro validation demonstrated that the halal marker gene's multiplex primer efficiently amplified the target sequence, specifically at the optimal annealing temperature of 58°C. Amplicons from beef (1,217 bp), pork (860 bp), rat (622 bp), and chicken (272 bp) primers could be distinguished on a 1.5% agarose gel. The study's results can aid in cost-effective and rapid halal testing and authentication of processed meat products, offering advantages over PCR with a single primer.
Co-Authors Andre Hendrawan Any Aryani Arsyam Mawardi Cut Nur Cinthia Alamanda Cut Nur Cinthia Alamanda Diah - Kusumawaty Diah Puspitasari Didik Priyandoko Dzikrina, Hanina Ema Rahmawati Euniche R.P.F. Ramandey Feraliana Feraliana Gusti Ayu Prani Pradani Gusti Ayu Prani Pradani I Dewa Agung Panji Dwipayana Ida Parwati Irin Annisa Evitayani Isak Solihin Kenia Permata Sukma Luhung Budiailmiawan Masduki, Fifi Fitriyah Nurul Faridah, Nurul Putri Cahya Destiani Rebecca Stephanie Reza Aditama Reza Aditama Rifky Waluyajati Rachman Rini Robiani Riyanti Weni Syafitri Rosalia Rani Ryan Bayusantika Ristandi Ryan Bayusantika Ristandi Sony Suhandono TRINA EKAWATI TALLEI Yana Maolana Syah Yeni Hotimah