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All Journal Indonesian Journal of Biotechnology BALANGA: Jurnal Pendidikan Teknologi dan Kejuruan
Refli
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Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Education Energy Engineering Immunology & microbiology Materials Science & Nanotechnology Other

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Expression analysis of antioxidant genes in response to drought stress in the fl ag leaf of two Indonesian rice cultivars R, Refli; Muljopawiro, Sukarti; Dewi, Kumala; Rachmawati, Diah
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.254 KB)

Abstract

The objective of this study was to analysis the expression of antioxidant genes in response to droughtstress in Indonesian rice. The malondialdehyde (MDA) content and the expression of Cu-ZnSod1, cCu-ZnSod2,MnSod1, cApxa, cApxb, chl-sApx, Cat1, Cat2, Cat3, Gr1, Gr2, and Gr3 genes were assayed in the rice fl ag leaf ofCiherang and Situ Bagendit cultivars subjected to control, mild and severe drought during the grain fi llingphase. Increase in MDA content of Ciherang treated to mild and severe drought was almost two-fold andthree-fold respectively, while MDA content in Situ Bagendit subjected to mild and severe drought increasedapproximately one-fold and two-fold as compared to the control. The semi quantitative reverse transcriptionpolymerase chain reaction (sqRT-PCR) analysis showed that the expression of cCu-ZnSod1, MnSod1, Cat2, Gr3genes of Ciherang, and cCu-ZnSod2, MnSod1, cApxa, cApxb, chl-sAPX, Cat2 and Gr1 genes of Situ Bagendit increasedin fl ag leaf of plant treated to drought. Expressions of cApxb, chl-sApx, Cat3 of Ciherang and Cu-ZnSod1 and Gr2genes of Situ Bagendit were not changed signifi cantly by drought stress. Decreased expression was shownby cCu-ZnSod2, cApxa, Cat1, Gr1 and Gr2 genes of Ciherang, and Cat1, Cat3 and Gr3 genes of Situ Bagendit. Theresults indicated that the activity of oxidative defense was regulated by four genes; cCu-ZnSod1, MnSod1, Cat2,Gr3 in Ciherang, and eight genes; cCu-ZnSod1, cCu-ZnSod2, MnSod1, cApxa, cApxb, chl-sApx, Cat2 and Gr1 in SituBagendit. Therefore, differences in the number of antioxidant genes controlling oxidative defense systemmight determine the difference of the oxidative defense capacity between both cultivars in response to droughtstress during grain fi lling.
COMMUNITY SERVICE PROGRAM (PKM): OPTIMIZATION OF 3R-BASED (REDUCE, REUSE, RECYCLE) WASTE MANAGEMENT AT THE MAPOLI WASTE BANK, KUPANG CITY Dwi Prasetyo; Alfred OM Dima; Refli; Novita Mariana Tuan; Saritha Kittie Uda
BALANGA: Jurnal Pendidikan Teknologi dan Kejuruan Vol. 13 No. 2 (2025): Journal Balanga Edisi Juli-Desember 2025
Publisher : Jurusan Pendidikan Teknologi dan Kejuruan, FKIP, Universitas Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/balanga.v13i2.23483

Abstract

The waste problem caused by low public awareness and limited waste management systems remains a crucial issue that leads to environmental degradation. This community service program was initiated as a solution to address the accumulation of unmanaged waste through the implementation of the 3R (Reduce, Reuse, Recycle) concept. Its objectives are to build community self-reliance in waste management, improve environmental quality, and empower local economic potential. Through outreach activities and intensive training, the program was implemented in Kuta Bangun Village. The results indicate the successful implementation of the program, marked by increased understanding and awareness among residents in applying proper waste management practices. In addition to raising awareness, this initiative also succeeded in establishing an independent waste-bank management group as a concrete step toward sustainability. The waste bank not only functions as a center for collecting and sorting waste but also serves as a driving force for the circular economy by converting recyclable inorganic waste into an additional source of income for residents. This success indicates that education- based interventions and community empowerment are effective in transforming behavior and creating a socially and economically sustainable waste management system at the village level.
Co-Authors Alfred OM Dima DIAH RACHMAWATI Dwi Prasetyo Kumala Dewi Novita Mariana Tuan Saritha Kittie Uda Sukarti Muljopawiro, Sukarti