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S Syekhfani
Department of Soil Science, Faculty of Agriculture, Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Characterization of phosphate solubilizing bacteria isolated from Pb contaminated soils and their potential for dissolving tricalcium phosphate Susilowati, L E; Syekhfani, S
Journal of Degraded and Mining Lands Management Vol. 1 No. 2 (2014)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2014.012.057

Abstract

Phosphorus solubilizing bacteria (PSB) enhances P availability in soils through dissolving inorganic P pools. The characteristics of PSB and the potential of three Pb tolerant phosphate solubilizing rhizobacteria, i.e. Pseudomonassp, Bacillus sp., and Actinomycites sp. were evaluated. PSB were isolated from soil samples contaminated with 300 and 500 mg Pb/kg after incubation for 30 days. Phosphate solubilizing bacteria were screened on for phosphate solubilisation ability in Pikovskaya agar medium (PA). In addition, two of the three indentified PSB strains (Pseudomonas sp. and Bacillus sp.) were characterized for their ability to solubilize tricalcium phosphate in Pikoskaya broth (PB) and also were examined their growth during culture medium incubation. The isolates exhibited different phosphate solubilization index, ranging from 1.87 to 2.98. Pseudomonas sp. had the highest ability to solubilize tricalcium phosphate: 9.82 mg P/ L and 12.23 mg P/ Lin Pikoskaya broth following the addition of 4 mg Pb/L and 2 mg /Pb L, respectively. 
Effects of application of groundnut biomass compost on uptake of phosphorus by maize grown on an Ultisol of South Sulawesi Kasifah, K; Syekhfani, S; Nuraini, Y; Handayanto, E
Journal of Degraded and Mining Lands Management Vol. 1 No. 4 (2014)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2014.014.159

Abstract

Low crop production is acid dryland area of South Sulawesi is due to low availability of P in the soils. One of alternatives that can be performed to overcome the problems of acid soils having high level of exchangeable Al, is through the addition of organic material. In the upland areas in South Sulawesi, crop rice, maize and groundnut crop residues are readily available, but the crop residues are generally only used as animal feed or even burned. This study was aimed to elucidate the effects of groundnut compost on P uptake by maize in Ultisol of Moncongloe, South Sulawesi. Eight kilograms of air dried soil was mixed with compost according to the following treatments; 0, 10, 15, 20, 25, 30, 35 and 40 t compost/ha. All pots received 200 kg/ha KCl and 300 kg Urea/ha as basal fertilizers. Two maize seeds were planted in each pot and thinned to one plant per pot after one week. At harvest maize shoot dry weight and maize root dry weight, length of maize cop, cob weight, cob diameter, weight grains per cob, P uptake by maize, P content in maize grain, soil available P were measured. Results of the study showed that groundnut compost has the ability to improve the availability of P in the soil and increase P uptake by maize grown on an Ultisol of South Sulawesi. Application of 25 t groundnut compost/ha was the optimal rate that can be used to increase P availability in an Ultisol of South Sulawesi.
The potential of phosphate solubilizing bacteria isolated from sugarcane wastes for solubilizing phosphate Atekan, A; Nuraini, Y; Handayanto, E; Syekhfani, S
Journal of Degraded and Mining Lands Management Vol. 1 No. 4 (2014)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2014.014.175

Abstract

Most of P in agricultural soils is in unavailable forms for plant growth. Phosphate solubilizing bacteria can increase soil P availability. This study was aimed to isolate phosphate solubilizing bacteria from sugarcane waste compost and to test ability of the isolated bacterial to dissolve phosphate. The bacteria were isolated from three types of sugarcane waste, i.e. filter cake compost, bagasse compost, and a mixture of filter cake + bagasse + trash biomass compost. The potential colony was further purified by the Pikovskaya method on selective media. Eight isolates of phosphate solubilizing bacteria were obtained from all wasted studied. Amongst them, T-K5 and T-K6 isolates were superior in dissolving P from Ca3(PO4)2 in the media studied. The two isolates were able to solubilize P with solubilizing index of 1.75 and 1.67 for T-K5 and T-K6, respectively. Quantitatively, T-K6 isolate showed the highest P solubilization (0.74 mg / L), followed by T-K5 isolate (0.56 mg / L), while the lowest P solubilization (0.41 mg / L) was observed for T-K4 isolate. The increase of soluble P was not always followed by the decrease in pH.
Effects of humic acid-based cation buffer on chemical characteristics of saline soil and growth of maize Mindari, W; Aini, N; Kusuma, Z; Syekhfani, S
Journal of Degraded and Mining Lands Management Vol. 2 No. 1 (2014)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2014.021.259

Abstract

Humic acid is believed to maintain the stability of the soil reaction, adsorption / fixation / cationic chelate, thereby increasing the availability of water and plant nutrients. On the other hand, the dynamics of saline soil cation is strongly influenced by the change of seasons that disrupt water and nutrient uptake of plants. This experiment was aimed to examine the characteristics of the humic acid from compost, coal, and peat and its function in the adsorption of cations (K+ and NH4+), thus increasing the availability of nutrients and growth of maize. The treatments tested were combination of three sources of humic acid (compost, peat and coal), two types of cation additives (K+ and NH4+), and three doses of humic acid-based buffer: 10, 20, and 30 g / 3kg. The treatments were evaluated against changes in pH, EC, cation exchange, chlorophyll content, plant dry weight and plant height. The experimental results showed that the addition of K+ and NH4+ affected the value of pH, CEC, K+, NH4+ and water content of the buffer. Application of humic acid-based buffer significantly affected exchangeable Na and exchangeable K. Humic pH dropped from > 7 to about 6.3, EC soil was about 0.9 mS / cm, exchangeable Ca, exchangeable Mg, exchangeable Na, and exchangeable K, respectively, were around 15.57-20:21; 1.76-6.52; 0.40-0.56 and 0.05-0.51 me / 100g soil. Plant growth (plant height, chlorophyll content, leaf area, and stem weight) at 35 days after planting increased with increasing dose of humic acid. The dose of 20g peat humic acid + NH4+ or 30g peat humic acid + K+ per 3 kg of saline soil gave the best results compared to the growth of maize from other treatments.
Effects of application of marine mud and manure on growth and yield of Capsicum annum L. on an Ultisol of Maluku Matulessy, F; Wardiyati, T; Syekhfani, S; Aini, N
Journal of Degraded and Mining Lands Management Vol. 2 No. 4 (2015)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2015.024.391

Abstract

Marine mud and manure are potential for improving ultisol conditions, especially soil acidity, CEC, base saturation, organic acid, soil structure, soil nutrient retention, aeration, soil humidity, water holding capacity, infiltration, and supply of phosphorus for plant growth and development. Two treatments, namely planting media with 200 t marine mud /ha + 30 t manure / ha, and 400 t marine mud /ha + 30 t manure / ha were able to increase pH from 4.6 to 5.6. A significant decrease of exchangeable Alsolubility of about 0.03 meq/100 g was found in M1O3; M2O1; M2O3 and M3O1 treatments. An increase of available phosphorus about 5.02 mg/kg was found at 200 t marine mud/ha + 30 t manure/ha treatment. There was a significant increase in plant height of about 62.42 cm in the media without marine mud and 30 t manure/ha. Application of 30 t manure/ hayielded plant with leaf size of about 9552 cm2 /plantand fresh fruit of about 9.81 t/ha. 
Co-Authors A Atekan E Handayanto F Matulessy, F kasifah L E Susilowati N Aini T Wardiyati W Mindari Y Nuraini Z Kusuma